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1.
人工感染新城疫病毒对雏鸡淋巴细胞功能的影响 总被引：4，自引：0，他引：4
确定了ＭＭＴ法测定鸡脾淋巴细胞转化实验的最佳条件，并用此法检测了人工感染新城疫病毒雏鸡和Ｌａ Ｓｏｔａ免疫雏鸡受到强毒攻击时脾淋巴细胞的转化率，实验表明，在两种情况下，雏鸡脾淋巴细胞对ＣｏｎＡ和ＰＨＡ的反应性与对照组比较有显著的差异，提示在新城疫感染和免疫中，淋巴细胞具有一定作用。 相似文献
2.
人工感染新城疫病毒引起LaSota疫苗免疫雏鸡细胞免疫功能及血… 总被引：4，自引：0，他引：4
以Ｌａ Ｓｏｔａ免疫雏鸡人工感染新城疫强毒前后Ｔ淋巴细胞免疫活性和血清ＩｇＧ抗体含量的动态变化进行了检测。实验表明,ＬａＳｏｔａ免疫鸡在强毒攻击前脾淋巴细胞对ＣｏｎＡ和ＰＨＡ均无反应性,但血清ＩｇＧ抗体的含量则逐渐升高。 相似文献
3.
对ＬａＳｏｔａ免疫雏鸡人工感染新城疫强毒前后Ｔ淋巴细胞免疫活性和血清ＩｇＧ抗体含量的动态变化进行了检测。实验表明，ＬａＳｏｔａ免疫鸡在强毒攻击前脾淋巴细胞对ＣｏｎＡ和ＰＨＡ均无反应性，但血清ＩｇＧ抗体的含量则逐渐升高。在受到强毒攻击后，脾淋巴细胞对ＣｏｎＡ和ＰＨＡ具有非常明显的反应性，而血清ＩｇＧ抗体起初下降，随后逐渐升高，这些结果揭示在新城疫的免疫和感染中，细胞免疫和体液免疫具有协同作用。 相似文献
4.
采用化学发光（ＣＬ）法测定雏鸡禽成髓细胞性白血病强毒（ｖＡＭＶ）感染后免疫器官淋巴细胞自由基产生水平的动态变化,结果表明雏鸡ｖＡＭＶ感染后淋巴细胞化学发光的变化不同于健康对照雏鸡,与ｖＡＭＶ致病过程直接相关。 相似文献
5.
人工感梁vMDV雏鸡IL—2活性和淋巴细胞增殖反应的变化 总被引：1，自引：0，他引：1
对１日龄ＡＡ肉用雏鸡人工感染马立克氏病强毒后,脾Ｔ淋巴细胞ＩＬ－２诱生活性和Ｔ淋巴细胞增殖反应减弱或显著减弱,;ＭＤ平均发率为３３．７５％,平均死亡率为１１．２５％。 相似文献
6.
鸡贫血病雏鸡ND免疫后局部免疫组织抗体生成细胞变化 总被引：1，自引：1，他引：0
对感染鸡贫血病毒（ＣＡＶ）雏鸡新城疫（ＮＤ）疫苗免疫及其强毒攻击后局部免疫组织——盲肠扁桃体和哈德尔腺的ＩｇＡ、ＩｇＧ和ＩｇＭ抗体生成细胞数量的动态变化进行了检测。结果发现,ＣＡＶ感染雏鸡ＮＤ免疫后７、１４、２８ｄ及新城疫强毒（ｖＮＤＶ）攻击后,上述免疫组织的三种抗体生成细胞数量均不同程度地低于未感染ＣＡＶ的ＮＤ免疫对照雏鸡。其中盲肠扁桃体弥散区的ＩｇＡ减少最为明显。表明ＣＡＶ感染雏鸡消化道和呼吸道局部免疫组织对新城疫疫苗的体液免疫应答功能降低。新城疫强毒攻击后ＣＡＶ感染ＮＤ免疫雏鸡的免疫保护率也明显低于未感染ＣＡＶ的免疫对照雏鸡。 相似文献
7.
感染网状内皮组织增殖病病毒SPF雏鸡中枢和外周免疫器官的免疫学变化 总被引：1，自引：1，他引：0
实验研究了１日龄ＳＰＦ雏鸡感染网状内皮组织增殖病病毒（Ｒｅｔｉｃｕｌｏｅｎｄｏｔｈｅｌｉｏｓｉｓｖｉｒｕｓ,ＲＥＶ）后免疫器官匀浆涂片的免疫学变化。结果发现,雏鸡感染ＲＥＶ后其免疫器官法氏囊、胸腺、脾脏中淋巴细胞数、酯酶阳性（ＡＮＡＥ＋）Ｔ细胞数以及颗粒型和弥散型ＡＮＡＥ＋Ｔ淋巴细胞数显著降低（Ｐ＜０．０５,Ｐ＜０．０１）。表明１日龄ＳＰＦ雏鸡感染ＲＥＶ后,其中枢免疫器官和外周免疫器官的细胞免疫呈现抑制。 相似文献
8.
对感染鸡传染性贫血病毒（ＣＩＡＶ）雏鸡新城疫（ＮＤ）免疫及其强毒攻击后，其免疫器官ｋｋ法氏囊、脾脏和胸腺的ＩｇＧ、ＩｇＭ和ＩｇＡ抗体生成细胞的动态变化进行了检测。结果发现，感染雏鸡ＩｇＧ抗体生成细胞于免疫后７～２８ｄ，在法氏囊和胸腺髓质及脾脏红髓和白髓区明显低于未感染的免疫对照雏鸡；ＩｇＭ生成细胞分别于免疫后７～２８ｄ、７ｄ或１４ｄ明显降低；而ＩｇＡ生成细胞于免疫后２８ｄ，仅在法氏囊髓质区明显减少，表明ＣＩＡＶ感染雏鸡免疫器官对ＮＤ免疫的体液免疫应答降低。ＮＤ强毒攻击后，ＣＩＡＶ感染ＮＤ免疫雏鸡法氏囊、脾脏、胸腺的三种抗体生成细胞均程度不同地低于对照雏鸡，其中ＩｇＧ生成细胞减少最为明显。ＣＩＡＶ感染雏鸡免疫器官抗体生成细胞减少与免疫保护率降低密切相关 相似文献
9.
本实验以马立克氏病（ＭＤ）三价苗（ＳＢ＿１＋８１４＋ＨＶＴ）和ＨＶＴ疫苗免疫１日龄肉用雏鸡，１５日龄时腹腔注射马立克氏病强毒（ｖＤＭＶ）进行攻击，在攻毒后５、２５、４５和７５天用间接ＥＬＩＳＡ法测定实验雏鸡血清、泪液、气管液、胆汁和肠液ＩｇＧ、ＩｇＭ和ｌｇＡ含量的动态变化，结果发现免疫雏鸡强毒攻击后，其血清、泪液和气管液的ＩｇＧ、ｌｇＭ和ＩｇＡ含量显著高于感染对照鸡，胆汁和肠液中ＩｇＡ以及ＩｇＧ、ＩｇＭ显著高于感染对照鸡，其中三价菌免疫鸡的３种免疫球蛋白含量明显高于ＨＶＴ免疫鸡；表明ＭＤ强毒攻击后，免疫雏鸡全身和消化道、呼吸道局部的体液免疫应答显著增强，三价苗免疫鸡的体液免疫反应明显高于ＨＶＴ免疫鸡，并与其较高的免疫保护率相关。 相似文献
10.
研究１日龄ＳＰＦ雏鸡感染网状内皮组织增殖病病毒后局部免疫组织均浆涂片的免疫学变化。结果表明：雏鸡感染ＲＥＶ后其局部免疫组织中，淋巴细胞数、ＡＮＡＥ、Ｔ细胞数以及颗粒型和弥散型ＡＮＡＥ＾＋Ｔ淋巴细胞数明显低于未感染雏鸡。说明病消化道和呼吸道相关的局部免疫组织的细胞免疫呈现抑制。 相似文献
11.
不同感染量REV对鸡免疫反应和细胞毒性作用的影响 总被引：4，自引：0，他引：4
用不同剂量网状内皮增生症病毒（REV）感染肉鸡和SPF鸡后，检测血液中T淋巴细胞对ConA的反应和NK细胞、细胞毒T细胞（CTL）的细胞毒性作用以及NDV抗体生成变化等，观察REV感染对机体非特异性、特异性细胞免疫反应和体液免疫反应的影响。结果表明无论高剂量还是低剂量REV感染均造成体液免疫和非特异性细胞免疫抑制，而且高剂量比低剂量对免疫功能的抑制作用更强，但对NK细胞和细胞毒T细胞的细胞杀伤活性却有升高趋势，在抗肿瘤方面发挥一定的作用。这种结果说明REV感染对机体的免疫抑制是有选择性的，且抑制程度与感染病毒量有关。 相似文献
12.
A recombinant fowlpox virus (rFPV) coexpressing the Newcastle disease virus (NDV) fusion and hemagglutinin-neuraminidase genes and infectious laryngothracheitis virus (ILTV) glycoprotein B gene was constructed. This virus was then evaluated for its ability to protect specific-pathogen-free (SPF) chickens against clinical symptoms and death after challenge by virulent NDV and ILTV. SPF chickens were grouped and vaccinated with the rFPV and commercial NDV (La Sota) and ILTV attenuated live vaccine (Nobilis ILT), respectively. After challenge with NDV 10 days postvaccination, 70% of chickens vaccinated with rFPV were protected from death, whereas 100% of the commercial NDV-vaccinated chickens were protected from death. In contrast, 100% of the unvaccinated chickens died after challenge. After challenge with ILTV, both the rFPV and commercial ILTV-vaccinated chickens were completely protected from death and 70% of chickens were protected from respiratory signs. In comparison, 100% of the unvaccinated chickens developed severe respiratory disease and 10% of chickens died. The protective efficacy was also measured by the antibody responses and isolation of challenge viruses. Results showed that this rFPV could be a potential vaccine for preventing NDV and ILTV by a single immunization. 相似文献
13.
本研究以新城疫病毒(NDV)V蛋白羧基端结构域(Vc)的重组蛋白为包被抗原,建立了用于检测NDV V蛋白抗体的间接ELISA方法,并采用该方法检测了鸡群免疫或接毒后血清中的V蛋白抗体水平。结果显示:两组不同NDV灭活疫苗组在免疫后的3周内检测结果均为阴性;两组灭活疫苗免疫3周后再人工感染NDV强毒的鸡群,攻毒后第7、14和21 d,NDV阳性率分别为60%、80%、70%和50%、80%、70%;两组不同的NDV弱毒疫苗免疫组鸡群,仅在免疫后第21 d阳性率分别为20%和10%。以上结果表明,NDV疫苗免疫组与强毒感染组的V蛋白抗体阳性率存在明显差异,本方法可在群体水平上区分新城疫疫苗免疫与强毒感染鸡群,为NDV血清学诊断和流行病学调查提供了一种新的检测手段。 相似文献
14.
Humorally deficient, in ovo-bursectomized (Bx) and sham-Bx chickens were vaccinated twice, 1 month apart, with Newcastle disease virus (NDV) Roakin strain and challenged with a velogenic viscerotropic NDV strain via the oronasal route. Hemagglutination-inhibition and seroneutralization tests showed that Bx chickens had reduced antibody-mediated immunity to virus infection. In contrast, they had significantly higher cell-mediated immunity (CMI) before challenge, as estimated simultaneously by determination of blastogenic capacity of peripheral blood lymphocytes induced by phytohemagglutinin and by specific antigen stimulation. After virus challenge, there was transitory inhibition of CMI based on marked reductions in levels of stimulation indices, and this impairment in CMI was supported by persistence of virus in Bx chickens for longer periods. Bx chickens resisted challenge, even though antibody titers were well below those considered predictive of resistance to challenge, suggesting that CMI provides a degree of resistance to velogenic NDV. 相似文献
15.
新城疫单抗ELISA试剂盒监测免疫鸡群中新城疫强毒 总被引：1，自引：0，他引：1
应用新城疫（ND）单抗ELISA试剂盒对2个免疫鸡群中ND强毒感染情况及个体感染强毒后排毒动态跟踪监测，并平等测定其HI抗体效价。共采集泄殖腔棉拭子和血液对应样品2317份。结果表明：HI效价2－14之间的个体均可检出强毒，其中HI效价在6以下和11以上的个体强毒检出率都较高。HI效价在6以上的个体仍角感染强毒，强毒感染导致HI抗体水平上升，且感染前低者上升速度较快，幅度亦较大；并且发现排毒个体的高水平抗体是强毒感染所致。个体感染强毒后排毒过程可长达3周，而且感染前抗体水平低者排毒时间相对较长。强毒一旦侵入鸡群便可在群内巡回传播，长期维持下来。 相似文献
16.
Naoyuki AIHARA Noriyuki HORIUCHI Nanase HIKICHI Mariko OCHIAI Yuko HOSODA Yoko ISHIKAWA Yoko SHIMAZAKI Koji OISHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(8):913-918
Infectious bursal disease (IBD) is characterized by immunosuppression due to the depletion of lymphocytes in the atrophied bursa of Fabricius (BF). We have sometimes encountered contradictory findings: chickens infected with the vaccine IBD virus (IBDV) strain have sometimes exhibited a highly atrophied BF, but not immunosuppression. In this study, chickens administered vaccine or wild-type strains of IBDV were later vaccinated with the B1 strain of the Newcastle disease virus (NDV). Bursal changes were examined histologically with a focus on the bursal follicle. The immunoreactivity to NDV was also evaluated with the hemagglutination inhibition test. In gross examination, we observed a few chickens with a severely atrophied BF in vaccine strain-administered groups (vaccine groups), and the level of severity was the same as that in the wild-type strain-administered group (wild-type group). However, these chickens retained humoral antibody responses to NDV and were revealed to possess a higher number of bursal follicles than those of the wild-type group. These results indicated that macroscopic evaluation dose not accurately reflect the immunoreactivity and degree of bursal damage in IBDV-administered chickens. We also found non-immunosuppressed chickens in the wild-type group. These non-immunosuppressed chickens retained a significantly higher number of normal follicles and total follicles according to our statistical analysis. Furthermore, a high correlation coefficient between the NDV-HI titer and the number of normal follicles was found in the wild-type group. These results implied that the retained number of normal follicles is important for the immunoreactivity of chickens infected with IBDV. 相似文献
17.
用马立克氏病病毒（MDV）感染5日龄SPF鸡后,取21日龄和35日龄鸡的淋巴细胞,运用3H-TdR掺入法检测T淋巴细胞对ConA和重组鸡白细胞介素18（rChIL-18）的反应,并用MTT法检测NK细胞和CTL对MD肿瘤细胞系CU147的杀伤活性及rChIL-18和IFN-γ对它们的作用。结果显示,SPF鸡感染MDV后淋转水平显著下降,NK细胞、CTL杀伤活性在感染后21日龄时升高,而在35日龄时NK细胞杀伤活性显著下降。rChIL-18对对照组和感染组SPF鸡的淋转水平和杀伤活性均有提高作用,同样IFN-γ也具有提高NK细胞和CTL杀伤活性的作用。 相似文献
18.
Munir K Muneer MA Tiwari A Chaudhry RM Muruganandan S 《Veterinary research communications》2007,31(7):909-929
Immunization against Angara disease virus (ADV), a serotype 4 avian adenovirus, and Newcastle disease virus (NDV), an avian
paramyxovirus serotype 1, is the mainstay of a broiler vaccination programme, while polyether ionophores usually form an essential
component of a broiler medication programme in most parts of India and Pakistan. The role of polyether ionophores in the protective
immune responses of broiler chickens vaccinated and challenged with ADV and NDV was investigated. A total of 1600 birds were
divided into eight groups of 200 birds each. First four groups were vaccinated against NDV and ADV, while the remaining four
served as unvaccinated controls. The first 3 groups of birds were administered salinomycin, monensin and cyclophosphamide
(CYP), respectively. The last group served as an untreated control. The same treatment schedule was also followed for the
next four unvaccinated groups. The post-vaccination and post-challenge serological responses to NDV and ADV, body and lymphoid
organ weight gains, post-challenge survival rate and detection of NDV and ADV in the tissues of infected birds were evaluated.
Birds administered salinomycin showed a significant stimulation of protective immune responses against both NDV and ADV as
compared to the untreated and CYP-treated birds. Monensin also enhanced the protective immune responses against both viruses
but the effect was not statistically significant. Thus, it is concluded that monensin and salinomycin augment the anti-NDV
and anti-ADV immune responses in broiler chickens, which supports their use in poultry flocks. 相似文献
19.
Specific-pathogen free (SPF) chickens were inoculated with the plasmid constructs encoding the fusion (F) and haemagglutinin-neuraminidase (HN) glycoproteins of Newcastle disease virus (NDV), either individually or in combination and challenged with velogenic NDV. The antibody level against NDV was measured using commercial enzyme linked immunosorbent assay (ELISA). In the first immunization regimen, SPF chickens inoculated twice with NDV-F or NDV-HN constructs elicited antibody responses 1 week after the second injection. However, the levels of the antibody were low and did not confer significant protection from the lethal challenge. In addition, administration of the plasmid constructs with Freund's adjuvant did not improve the level of protection. In the second immunization regimen, chickens inoculated twice with the plasmid constructs emulsified with Freund's adjuvant induced significant antibody titers after the third injection. Three out of nine (33.3%) chickens vaccinated with pEGFP-HN, five of ten (50.0%) chickens vaccinated with pEGFP-F and nine of ten (90.0%) chickens vaccinated with combined pEGFP-F and pEGFP-HN were protected from the challenge. No significant differences in the levels of protection were observed when the chickens were vaccinated with linearized pEGFP-F. The results suggested that more than two injections with both F and HN encoding plasmid DNA were required to induce higher level of antibodies for protection against velogenic NDV in chickens. 相似文献
20.
A whole-blood-culture technique was used to sequentially evaluated peripheral blood lymphocyte responses to phytohemagglutinin (PHA) and concanavalin A (Con A) of normal chickens and chickens infected at 1 day or 3 weeks of age with infectious bursal disease virus (IBDV). This method had numerous advantages over the more conventional techniques. A comparative study was made on the percentage of inhibition of responses of peripheral blood lymphocytes to PHA and Con A of 1-day- and 3-week-old IBDV-infected chickens. In both groups, there was a minimum inhibition between 3 and 4 weeks postinfection (PI) and a maximum inhibition at 6 weeks PI. A one-way mixed lymphocyte reaction (MLR) was performed using mitomycin-C-treated cells as stimulator cells obtained from chickens of genetically different strains. Lymphocytes from the experimental birds (control, 1-day-infected, and 3-week-infected groups) were used as the responder cells. The results showed that MLR response of the IBDV-infected chickens was significantly reduced compared with those of the uninfected controls. The degree of lowered response was much more severe in chickens infected at 1 day of age than in those infected at 3 weeks of age. 相似文献