共查询到20条相似文献，搜索用时 37 毫秒
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In vitro transcription enhancement by purified derivatives of the glucocorticoid receptor 总被引：15，自引：0，他引：15
L P Freedman S K Yoshinaga J N Vanderbilt K R Yamamoto 《Science (New York, N.Y.)》1989,245(4915):298-301
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Cloning of a factor required for activity of the Ah (dioxin) receptor. 总被引：56，自引：0，他引：56
E C Hoffman H Reyes F F Chu F Sander L H Conley B A Brooks O Hankinson 《Science (New York, N.Y.)》1991,252(5008):954-958
The aryl hydrocarbon (Ah) receptor binds various environmental pollutants, such as polycyclic aromatic hydrocarbons, heterocyclic amines, and polychlorinated aromatic compounds (dioxins, dibenzofurans, and biphenyls), and mediates the carcinogenic effects of these agents. The complementary DNA and part of the gene for an 87-kilodalton human protein that is necessary for Ah receptor function have been cloned. The protein is not the ligand-binding subunit of the receptor but is a factor that is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after binding ligand. The requirement for this factor distinguishes the Ah receptor from the glucocorticoid receptor, to which the Ah receptor has been presumed to be similar. Two portions of the 87-kilodalton protein share sequence similarities with two Drosophila proteins, Per and Sim. Another segment of the protein shows conformity to the consensus sequence for the basic helix-loop-helix motif found in proteins that bind DNA as homodimers or heterodimers. 相似文献
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Negative regulation by glucocorticoids through interference with a cAMP responsive enhancer 总被引：65，自引：0，他引：65
I E Akerblom E P Slater M Beato J D Baxter P L Mellon 《Science (New York, N.Y.)》1988,241(4863):350-353
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【目的】利用正负筛选策略,构建猪肌肉生长抑素（Myostatin,MSTN）基因的双筛选标记打靶载体。【方法】以猪胎儿成纤维细胞DNA为模板,PCR扩增MSTN基因同源长、短臂;采用PCR和Overlap PCR扩增打靶载体正筛选标记嘌呤霉素和绿色荧光蛋白基因及负筛选标记单纯疱疹病毒胸苷激酶基因。以pUC57载体为骨架载体,在其多克隆位点连接一段包括Frt序列在内的酶切位点的多克隆位点序列,在2个Frt序列之间连接正筛选标记嘌呤霉素基因和绿色荧光蛋白基因,在Frt序列两侧分别连接5.7和1.9 kb的MSTN基因同源长、短臂;在同源短臂后连接负筛选标记单纯疱疹病毒胸苷激酶基因,将目的片段与载体定向连接克隆。用脂质体法将打靶载体转染猪肾细胞（PK15细胞）,用嘌呤霉素和丙氧鸟苷进行正负筛选,验证正负筛选标记基因的功能。【结果】成功克隆了猪MSTN基因同源长、短臂及正筛选嘌呤霉素和绿色荧光蛋白基因及负筛选单纯疱疹病毒胸苷激酶基因,构建了猪MSTN基因双筛选标记打靶载体,打靶载体长14 kb。在打靶载体转染的PK15细胞中,正负筛选标记基因均有生物学活性。【结论】成功构建了猪MSTN基因双筛选标记打靶载体。 相似文献
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1,25-dihydroxyvitamin D-responsive element and glucocorticoid repression in the osteocalcin gene 总被引：29，自引：0，他引：29
N A Morrison J Shine J C Fragonas V Verkest M L McMenemy J A Eisman 《Science (New York, N.Y.)》1989,246(4934):1158-1161
The active hormonal form of vitamin D3, 1,25-dihydroxyvitamin D3[1,25(OH), which regulates cellular replication and function in many tissues and has a role in bone and calcium homeostasis, acts through a hormone receptor homologous with other steroid and thyroid hormone receptors. A 1,25(OH)2D3-responsive element (VDRE), which is within the promoter for osteocalcin [a bone protein induced by 1,25(OH)2D3] is unresponsive to other steroid hormones, can function in a heterologous promoter, and contains a doubly palindromic DNA sequence (TTGGTGACTCACCGGGTGAAC; -513 to -493 bp), with nucleotide sequence homology to other hormone responsive elements. The potent glucocorticoid repression of 1,25(OH)2D3 induction and of basal activity of this promoter acts through a region between -196 and +34 bp, distinct from the VDRE. 相似文献
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Molecular cloning of the thyrotropin receptor 总被引：35，自引：0，他引：35
M Parmentier F Libert C Maenhaut A Lefort C Gérard J Perret J Van Sande J E Dumont G Vassart 《Science (New York, N.Y.)》1989,246(4937):1620-1622
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Molecular cloning of the chicken progesterone receptor 总被引：16，自引：0，他引：16
O M Conneely W P Sullivan D O Toft M Birnbaumer R G Cook B L Maxwell T Zarucki-Schulz G L Greene W T Schrader B W O'Malley 《Science (New York, N.Y.)》1986,233(4765):767-770
To define the functional domains of the progesterone receptor required for gene regulation, complementary DNA (cDNA) clones encoding the chicken progesterone receptor have been isolated from a chicken oviduct lambda gt11 cDNA expression library. Positive clones expressed antigenic determinants that cross-reacted with six monospecific antibodies derived from two independent sources. A 36-amino acid peptide sequence obtained by microsequencing of purified progesterone receptor was encoded by nucleotide sequences in the longest cDNA clone. Analysis of the amino acid sequence of the progesterone receptor deduced from the cDNA clones revealed a cysteine-rich region that was homologous to a region found in the estrogen and glucocorticoid receptors and to the avian erythroblastosis virus gag-erb-A fusion protein. Northern blot analysis with chicken progesterone receptor cDNA's indicated the existence of at least three messenger RNA species. These messages were found only in oviduct and could be induced by estrogens. 相似文献
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HTLV-I tax induces cellular proteins that activate the kappa B element in the IL-2 receptor alpha gene 总被引：91，自引：0，他引：91
D W Ballard E B?hnlein J W Lowenthal Y Wano B R Franza W C Greene 《Science (New York, N.Y.)》1988,241(4873):1652-1655
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Identification of a zinc finger protein that binds to the sterol regulatory element 总被引：36，自引：0，他引：36
T B Rajavashisth A K Taylor A Andalibi K L Svenson A J Lusis 《Science (New York, N.Y.)》1989,245(4918):640-643
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Solution structure of the glucocorticoid receptor DNA-binding domain 总被引：48，自引：0，他引：48
T H?rd E Kellenbach R Boelens B A Maler K Dahlman L P Freedman J Carlstedt-Duke K R Yamamoto J A Gustafsson R Kaptein 《Science (New York, N.Y.)》1990,249(4965):157-160
The three-dimensional structure of the DNA-binding domain (DBD) of the glucocorticoid receptor has been determined by nuclear magnetic resonance spectroscopy and distance geometry. The structure of a 71-residue protein fragment containing two "zinc finger" domains is based on a large set of proton-proton distances derived from nuclear Overhauser enhancement spectra, hydrogen bonds in previously identified secondary structure elements, and coordination of two zinc atoms by conserved cysteine residues. The DBD is found to consist of a globular body from which the finger regions extend. A model of the dimeric complex between the DBD and the glucocorticoid response element is proposed. The model is consistent with previous results indicating that specific amino acid residues of the DBD are involved in protein-DNA and protein-protein interactions. 相似文献
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The c-fos serum response element (SRE) is a primary nuclear target for intracellular signal transduction pathways triggered by growth factors. It is the target for both protein kinase C (PKC)-dependent and -independent signals. Function of the SRE requires binding of a cellular protein, termed serum response factor (SRF). A second protein, p62TCF, recognizes the SRE-SRF complex to form a ternary complex. A mutated SRE that bound SRF but failed to form the ternary complex selectively lost response to PKC activators, but retained response to PKC-independent signals. Thus, two different signaling pathways act through discrete nuclear targets at the SRE. At least one of these pathways functions by recruitment of a pathway-specific accessory factor (p62TCF). These results offer a molecular mechanism to account for the biological specificity of signals that appear to act through common DNA sequence elements. 相似文献
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D Pennica W J Kohr W J Kuang D Glaister B B Aggarwal E Y Chen D V Goeddel 《Science (New York, N.Y.)》1987,236(4797):83-88
The primary structure of human uromodulin, a 616-amino acid, 85-kilodalton glycoprotein with in vitro immunosuppressive properties, was determined through isolation and characterization of complementary DNA and genomic clones. The amino acid sequence encoded by one of the exons of the uromodulin gene has homology to the low-density-lipoprotein receptor and the epidermal growth factor precursor. Northern hybridization analyses demonstrate that uromodulin is synthesized by the kidney. Evidence is provided that uromodulin is identical to the previously characterized Tamm-Horsfall glycoprotein, the most abundant protein in normal human urine. 相似文献