共查询到18条相似文献，搜索用时 421 毫秒
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应用Sry-PCR扩增鉴定狍(Capreolus capreolus)的性别 总被引：2，自引：0，他引：2
根据人的SRY基因核心序列设计合成1对引物C1、C2，应用PCR技术对野生狍(Capreolus capreolus)Sry基因(哺乳动物Y染色体DNA雄性特异区)进行扩增．结果在野生狍雄性样本扩增出1条带(221bp)．而在雌性样本未见扩增带．显示了Sry基因的性别特异性。应用这1对引物对42个未知性别狍肌肉组织样本进行了性别鉴定．结果雄性22个，雌性20个。对Sry-PCR产物克隆测序，得到185bp的Sry基因部分核苷酸序列。本试验为狍种群性别比率及其种群动态变化机制研究提供了资料。 相似文献
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《黑龙江畜牧兽医》2015,(9)
为了提高聚合酶链式反应(PCR)在小鼠雄性胚胎性别鉴定中的应用效果,试验利用单一扩增雄性特有Sry基因法选取60枚雄性胚胎,将其中40枚平均分为2组,试验组1利用双重基因扩增法进行复检,试验组2利用两步双重基因扩增法进行复检,比较这2种方法对雄性胚胎的鉴定效果;利用两步双重基因扩增法分别以1,2,3个卵裂球模板量对其余20枚雄性胚胎进行体外扩增,确定卵裂球的适宜使用量。结果表明:利用两步双重基因扩增法进行雄性胚胎判定的准确率为100%,显著高于双重基因扩增法的准确率(85.00%)(P0.05)。以3个卵裂球作为模板时的准确率为100%,可以成功复检所有雄性胚胎。因此,利用两步双重基因扩增法可以有效对小鼠雄性胚胎进行性别鉴定。 相似文献
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伦敦消息,对雄性遗传根源的探索已经完成。英国科学家确信他们已找到哺乳动物发育过程中,使雌性转变为雄性的关键基因。从而结束了近30年对性别决定基因的探索。这是一个简单而非凡的实验:向正常的携带有一对X染色体的雌性小鼠胚胎内注入Y染色体DNA上带有Sry基因(Short for sex-determining region Y gene,简称性别决定区Y基因)的一小片段,结果这些雌性胚胎发育为具 相似文献
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火烈鸟性别鉴定的分子标记方法 总被引：1，自引：0，他引：1
由于火烈鸟在外观上雌雄极为相似,很难用常规方法对其性别进行鉴定。本试验首次确立了利用PCR技术对火烈鸟性别进行鉴定的分子标记方法。从火烈鸟血液中提取总基因组DNA,针对性染色体上的CHD基因和EE0.6序列,筛选了4对引物进行特异性扩增,其中3对引物组合成2个组(A/B和B/C)进行多重PCR。结果表明,3组引物都在雄性个体中扩增出1条片段,在雌性中扩增出2条片段;待测群体中雄性个体11只,雌性个体6只。经检验,利用3组引物都能准确鉴定火烈鸟性别,为该物种在分子水平上的性别鉴定奠定了基础。 相似文献
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《中国家禽》2021,(9)
性别决定(Sex determination)是指有性生物个体在性别决定基因的作用下,经过一系列生物发育过程后,原始性腺发育成卵巢或睾丸并使生物体发育为雌性或雄性的过程。在胚胎发育过程中,性别分化由少数的关键基因决定,当一个或多个关键基因出现功能失调时,可能会导致性腺发育异常甚至发生性别逆转的现象。近年来,大量数据证明,DMRT1和FOXL2在多个物种中被证实为与性别决定相关的关键基因,然而两者在性别调控过程中的具体作用尚不十分清楚。因此,文章对DMRT1和FOXL2基因在多个物种性别决定中的研究进展进行综述与展望,为进一步探索与研究家禽性别的分化提供新思路。 相似文献
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White-tailed deer are susceptible to heartwater (Ehrlichia [Cowdria] ruminantium infection) and are likely to suffer high mortality if the disease spreads to the United States. It is vital, therefore, to validate a highly specific and sensitive detection method for E. ruminantium infection that can be reliably used in testing white-tailed deer, which are reservoirs of antigenically or genetically related agents such as Ehrlichia chaffeensis, Anaplasma (Ehrlichia) phagocytophilum (HGE agent) and Ehrlichia ewingii. Recently, a novel but as yet unnamed ehrlichial species, the white-tailed deer ehrlichia (WTDE), has been discovered in deer populations in the United States. Although the significance of WTDE as a pathogen is unknown at present, it can be distinguished from other Ehrlichia spp. based on 16S rRNA gene sequence analysis. In this study it was differentiated from E. ruminantium by the use of the pCS20 PCR assay which has high specificity and sensitivity for the detection of E. ruminantium. This assay did not amplify DNA from the WTDE DNA samples isolated from deer resident in Florida, Georgia and Missouri, but amplified the specific 279 bp fragment from E. ruminantium DNA. The specificity of the pCS20 PCR assay for E. ruminantium was confirmed by Southern hybridization. Similarly, the 16S PCR primers (nested) that amplify a specific 405-412 bp fragment from the WTDE DNA samples, did not amplify any product from E. ruminantium DNA. This result demonstrates that it would be possible to differentiate between E. ruminantium and the novel WTDE agent found in white tailed deer by applying the two respective PCR assays followed by Southern hybridizations. Since the pCS20 PCR assay also does not amplify any DNA products from E. chaffeensis or Ehrlichia canis DNA, it is therefore the method of choice for the detection of E. ruminantium in these deer and other animal hosts. 相似文献
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Kroly Erdlyi Lszl Dencs&#x; Rbert Lehoczki Mikls Heltai Krisztina Sonkoly Sndor Csnyi Norbert Solymosi 《Veterinary microbiology》2009,138(1-2):20-26
Roe deer papillomavirus (CcPV1) infection has been identified as an endemic disease in roe deer populations of the Carpathian basin in Central Europe (Hungary, Austria and Croatia). The disease is characterised by easily recognizable skin tumours similar to deer papillomavirus infection of North American deer species. In 2006, a questionnaire study was conducted among all Hungarian game management units (GMUs) in order to assess the distribution of the disease and its major epidemiological features. Categorical information was collected about disease occurrence, trend and frequency of detection, on primarily affected age classes in both sexes, and association of lesions with mortality. Replies were received from 539 GMUs representing 50.9% of total GMU territory and disease presence was reported by 295 (54.7%) GMUs. Older age classes of both sexes were found to be more affected. Association of various environmental factors with disease occurrence was evaluated and data were collected on the occurrence of similar skin lesions in other European countries. Pathological features of CcPV1 infection were described and the localisation of both CcPV1 antigen and DNA was characterised by immunohistochemistry and in situ DNA hybridisation in skin lesions. Virus presence was also demonstrated by PCR and PCR product sequencing. 相似文献
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Tamamoto C Seino N Suzuki M Kaji K Takahashi H Inokuma H 《Veterinary parasitology》2007,150(4):370-373
Ehrlichia muris DNA was detected in the blood of sika deer (Cervus nippon yesoensis) by species-specific PCR based on the citrate synthase gene, which was shown to be more sensitive than species-specific PCR based on the 16S rRNA gene. Among 102 deer examined, one deer was positive. Deer may be a possible mammalian reservoir of E. muris. 相似文献
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Expression of MPB70 Protein and Identification the Immunogenicity of Deer Mycobacterium tuberculosis
In this study,the immunogenicity protein MPB70 gene was amplified from Mycobacterium tuberculosis genome DNA which separated from deer, and about 590 bp fragment was obtained. Then the fragment was cloned and constructed prokaryotic expression vector of pET-30a-MPB70, and the recombinant plasmid was put into E. coli BL21(DE3).Purified after IPTG induction, and analyzed by SDS-PAGE, a specificity protein band was observed at 20 ku. Using the deer serum positive of tuberculosis in Western blotting, the fusion protein could be combined with deer serum positive of tuberculosis antibody and arise specific immune response. The protein could be used as a specific antigen to test the deer tuberculosis. The study laid a foundation for further studying the deer tuberculosis appraisal method. 相似文献
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本研究以分离的鹿结核分枝杆菌DNA为模板扩增免疫原性蛋白MPB70基因,获得约590 bp片段,并将其克隆,构建原核表达载体pET-30a-MPB70,将重组质粒转入大肠杆菌BL21（DE3）,经IPTG诱导后纯化和SDS-PAGE分析,在20 ku处可见特异性蛋白条带。利用鹿结核阳性血清进行Western blotting鉴定,原核表达的融合蛋白可与鹿结核阳性血清抗体结合,并出现特异的免疫反应。该蛋白可作为特异性抗原进行鹿结核病的检测,从而为鹿结核病诊断方法的研究奠定基础。 相似文献
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Hong Li Arno Wunschmann Janice Keller D Greg Hall Timothy B Crawford 《Journal of veterinary diagnostic investigation》2003,15(1):46-49
A subacute disease presenting primarily as alopecia and weight loss occurred in 2 white-tailed deer (Odocoileus virginianus) on farms in Minnesota and in Texas. A presumptive diagnosis of malignant catarrhal fever (MCF) was made on the basis of histological lesions. Antibody against an epitope conserved among the MCF group viruses was detected in the serum of both deer. DNA samples from the deer were subjected to a variety of PCR amplifications. Alignment of the amplified sequences from the diseased animals revealed that they were 100% identical to each other and to the same DNA fragment from the newly recognized member of the MCF virus group endemic in domestic goats (Capra hircus), provisionally named caprine herpesvirus 2 (CpHV-2). A seroprevalence survey from one of the deer farms showed a high rate of subclincal infection in the deer population. This study provides further confirmation that CpHV-2 is a pathogen, at least for deer, and emphasizes the risk of loss from MCF when mixing cervids with goats. 相似文献
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牙釉质基因鉴定麇鹿性别 总被引：1，自引：0，他引：1
性别鉴定是调查野生种群雌雄性比的重要方法,对野生动物种群管理具有重要意义。而牙釉质(AMEL)基因在性染色体上具有较高的保守性,在鉴定动物性别方面得到了应用,本次试验采用北京麋鹿生态实验中心的15头糜鹿组织样本,其中11个来自雄性麇鹿鹿茸,4个来自雌性糜鹿静脉血液。对所取样本分别进行基因组DNA提取、AMEL基因片段PCR扩增、纯化、测序。得到了AMEL基因鉴定和实际雌雄性别个数差异不显著(P>0.05)。结果表明:雌性麋鹿产生1条322 bpX带和1条N带,雄性麋鹿则产生322 bpX带和277 bpY带以及1条N带,雄鹿(10/10)和雌鹿(4/4)性别鉴定结果分别都与实际性别符合,所以,使用鹿茸角和血液样本进行AMEL.基因扩增电泳分析可以对糜鹿性别鉴定。 相似文献