共查询到20条相似文献，搜索用时 93 毫秒
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[目的]研究用荧光分光光度法测定水中苯酚和对苯二酚的含量,为环境样品中痕量物质的分析提供测定方法。[方法]取水样,加入β-环糊精溶液、EDTA溶液及缓冲溶液后测定。[结果]在波长对为λEx/λEm=240 nm/309 nm时测定苯酚含量,线性范围为0～1×10-4mol/L,检出限为6.6×10-8mol/L;在波长对为λEx/λEm=310 nm/340 nm时测定对苯二酚含量,线性范围为0～1.5×10-5mol/L,检出限为5.2×10-9mol/L。[结论]通过三维荧光扫描来选择干扰少而测定灵敏度较高的检测波长,实现了荧光分光光度法直接测定水样中的苯酚和对苯二酚含量。 相似文献
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采用荧光猝灭光谱、同步荧光光谱和紫外-可见吸收光谱,研究了白藜芦醇与牛血清白蛋白(BSA)的相互作用。结果表明白藜芦醇对BSA有较强的荧光猝灭作用,荧光猝灭机理属于二者形成复合物所引起的静态猝灭和非辐射能量转移;根据不同温度下防己诺林碱对BSA的荧光猝灭作用,利用Stern-Volmer方程处理实验数据,得到白藜芦醇与BSA之间的结合常数KA为6.11×104(25℃)、4.46×104(35℃)和3.84×104(45℃)。根据F rster非辐射能量转移理论,求出了白藜芦醇与BSA之间的结合距离为2.36 nm(25℃)、2.73 nm(35℃)和2.48 nm(45℃)。通过计算热力学参数,可知该药物与牛血清白蛋白的相互作用是一个吉布斯自由能降低的自发过程,且二者之间的主要作用力类型为疏水作用力,同时用同步荧光光谱探讨了白藜芦醇对BSA构象的影响。 相似文献
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硒化锌量子点作探针荧光法检测农药敌磺钠 总被引：3，自引：0，他引：3
以谷胱甘肽(GSH)为稳定剂,在水相中合成了高荧光的硒化锌量子点(ZnSe QDs).实验发现,农药敌磺钠能显著猝灭ZnSe QDs的荧光,同时采用荧光光谱和吸收光谱研究了敌磺钠与ZnSe QDs相互作用的荧光猝灭机理.通过对比3个温度下体系的Stern-Volmer方程动态猝灭常数KSV发现,敌磺钠对ZnSe QDs的荧光猝灭主要为动态过程.利用二者的猝灭作用建立了对农药敌磺钠的高灵敏检测新方法,其线性范围为6.69×10-7～2.23×10-4 mol/L,相关系数为R=0.999,检出限为2.01×10-7 mol/L.用该方法对自来水中残留农药进行检测,加标回收率在95.8%～105%之间. 相似文献
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测定痕量锗的高灵敏度显色反应的研究 总被引：2，自引：0，他引：2
本文较详细地研究了锗—对二甲氨基苯基荧光酮—十六烷基三甲基溴化铵显色体系水相光度法则定痕量锗的新方法。络合物的最大吸收峰在504mm 处,表观摩尔吸光系数为1.52×10~5L·mol~(-1)·cm~(-1),Sandell 灵敏度为4.77×10~(-4)μgG_e/cm~2。锗(Ⅳ)含量在0～7μg/25mL 范围内遵守比尔定律。方法用予煤炭中痕量锗的测定,结果满意。 相似文献
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研究发现钴铁氧体纳米粒子可催化H2O2氧化对甲苯酚产生荧光物质2,2’-二羟基-4,4’-二甲基联苯,据此建立了测定H2O2的方法.在优化条件下,荧光强度与H2O2浓度在1×10-91×10-6 mol/L范围内呈现良好的线性关系,H2O2的检出限为4.9×10-10 mol/L,对浓度为1×10-7 mol/L和1×10-6 mol/L的H2O2平行测定11次,其相对标准偏差（RSD）分别为3.5%和2.1%.该方法已成功用于测定雨水、自来水和江水等水样中的H2O2. 相似文献
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鱼腥草素同系物对巨噬细胞膜蛋白荧光特性的影响研究 总被引：1，自引：0，他引：1
研究了鱼腥草素同系物(HOU-Cn)对巨噬细胞膜蛋白构象的影响.荧光分析表明鱼腥草素在浓度低于1.6×10-4 mol/L范围内,随着浓度的上升,能迅速地淬灭酪氨酸残基的发射荧光强度.每个巨噬细胞可以结合1.70×1017分子的HOU-Cn,且随着碳链的延长,结合数略有增加.结果表明HOU-Cn通过疏水作用与巨噬细胞膜蛋白结合,由此改变膜蛋白的构象和激活巨噬细胞. 相似文献
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本文以荧光法研究了四环素（TC）与-环糊精（β-CD）的包合作用,对包合物形成机理进行了研究,并利用β-CD对TC荧光的增敏作用建立了1种高灵敏度测定TC的新方法,TC含量2.7×10-7~8.1×10-6mol.L-1范围内与荧光强度呈线性,方法的检出限为1.14×10-9mol.L-1,方法用于片剂中四环素含量测定,结果满意,回收率在95%~105%之间。 相似文献
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大白菜自交不亲和基因的利用 总被引：2，自引：0，他引：2
蕾期自花授粉试验结果表明大白菜(Brassica
campestris L. ssp.Pekinesis (Lour) Olsson)品系40-9所结种子最多,达0.165 g/株.利用种子结实分析方法和荧光显微镜技术对9个大白菜品系进行自交不亲和性测定,试验采取完全随机设计.用种子结实分析方法测定时,2个品系
27-3-7和40-9表现为自交不亲和系.用荧光显微镜技术测定时,品系23-3-4,27,27-3-7,40-9和142发现为弱性自交不亲和.用4个具有自交不亲和性的自交系进行正反交生产种子(由蜜蜂授粉),结果表明,40-9做母本结籽最多,这是因为母体效应.组合40-9×142-5,
40-9×27-3-7 和40-9×23-3-4结籽较多,分别为30.16,24.63和16.96 kg/ hm2.1998年在泰国清迈大学对11个大白菜杂一代品种和3个商用品种进行比较试验,一代组合142-5×40-9结球产量最高,达38
563 kg/ hm2,高于商用品种的36.3 %.其他组合如23×27, 23×142, 27-3-7×23-3-4,
27-3-7×142-5和40-9×23-3-4也表现较好的园艺性状,产量分别为29038,34694,22906,32188和23
656 kg/ hm2.但由于结球形状稍圆,市场销售尚不理想. 相似文献
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根据荧光分析技术,对奶牛血清的荧光特性进行了研究,研究了正常和异常奶牛血清经不同的光激发后所产生的荧光及其变化情况。初步结果表明,异常奶牛血清的荧光强度比正常奶牛血清的荧光强度要弱。提出通过对奶牛血清的荧光强度进行分析,将其应用于奶牛疾病诊断这一国内外较少涉足的研究课题。 相似文献
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荧光定量PCR技术研究进展及其应用 总被引：3，自引：0，他引：3
荧光定量PCR技术不仅实现了PCR从定性到定量的飞跃,更以其特异性强、灵敏度高、重复性好、定量准确、速度快、全封闭反应等优点而成为分子生物学研究的重要工具,并用于生物特异核苷酸的检测.主要概述了荧光染料法、TaqMan探针法、分子信标法、Lux Primer 4种荧光定量技术的原理、特点以及荧光定量PCR技术在各方面的应用. 相似文献
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以百日草3～4叶期的穴盘苗为试验材料,分析了高温胁迫下不同浓度(10,20,30,40 mg／L)烯效唑(S3307)对百日草幼苗的同化产物分配、光合作用、水分利用率、叶绿素荧光参数等指标的影响。结果表明:高温胁迫下S3307处理的百日草幼苗的株高降低,茎粗增加,地上部冠干重、地下部根干重及其根冠比均大于对照,并且S3307处理还提高了百日草幼苗的净光合速率、叶片水分利用率,显著减缓了高温胁迫对百日草幼苗光合系统Ⅱ(PSⅡ)的抑制作用,使百日草幼苗PSⅡ最大光化学效率(Fv／Fm)、实际光化学效率(ΦPSⅡ)、光化学猝灭系数(qP)下降缓慢,非光化学猝灭系数(NPQ)升高幅度增大,从而使光合机构免受高温胁迫的伤害。以30 mg／L的S3307处理效果最佳。 相似文献
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以阳生植物月季、玫瑰和阴生植物酢浆草、半夏为试验材料,利用IMAGING-PAM MINI版WALZ新型调制叶绿素荧光成像系统和QE65000光谱仪对4种植物叶片的叶绿素荧光参数和荧光光谱进行了测定,分析了阴生植物与阳生植物光化学活性与荧光光谱的差异.结果表明,与阳生植物相比,阴生植物叶片测定的叶绿素荧光参数中,光合系统Ⅱ的实际光化学效率、原初光化学效率和电子传递速率较高,而调节性能量耗散的光化学效率、非调节性能量耗散的光化学效率、光化学性能指数、最大荧光值和初始荧光值均较低;4种植物在650～800nm波长处的荧光光谱图形相同,最高峰均出现在680 nm附近,在735 nm处伴有一弱峰出现,并且阳生植物的Fp680/Fp735比阴生植物低. 相似文献
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Preliminary Study on the Photosynthetic Characteristics of Super Hybrid Rice P88S/0389 总被引：2，自引：0，他引：2
ZHANG Ji-zhen MENG Wei-dong CAO Sai DENG Xiao-feng .Key Laboratory of Chinese Ministry of Education for Tropical Biological Resources Hainan University Public Experiment Center of Hainan University Center for Experimental Biotechnology of Hainan University Haikou .Research Institute of Foodstuff Crop HAAS Haikou .Biotechnology 《（《农业科学与技术》）编辑部》2008,(4)
[Objective] The aim of this study is to understand the high yield often photosynthetic mechanism of super hybrid rice P88S/0389.[Method] Chlorophyll and carotenoid contents were measured via the method of Wellburn and Lichtenthaler,and the photosynthetic rate and chlorophyll fluorescence parameters were measured using portable photosynthesis system.Through comparison of above measured indices between P88S/0389 and its parents,the photosynthetic characteristics were obtained.[Result] The pigment content of P88S/0389 lied between their parents,and detailedly was closer to its female parent at earlier growth stage and to its male parent at latter stage,respectively.The Pn,Fv/Fo,Fv/Fm and qP of the flag leaf of P88S/0389 were all higher than those of its parents,while the degradation rates of these parameters were slower than those of its parents.The qN of flag leaf was lower than those of its parents,and the increasing rate of qN of P88S/0389 was also slower than those of its parents,indicating the great heterosis of P88S/0389 to its parents in photosynthetic capacity.The data show that P88S/0389 is endowed with stronger capacities in light energy absorption,transformation and carbon fixation compared to its parents.[Conclusion] The light energy absorbed by hybrid mainly contributes to photochemical reaction,and little for heat dissipation.For ensuring high yield,the rice cultivars selected for production should synchronously possess higher photosynthetic rate and longer high value duration. 相似文献
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《（《农业科学与技术》）编辑部》2008,(4)
[Objective] The aim of this study is to understand the high yield often photosynthetic mechanism of super hybrid rice P88S/0389.[Method] Chlorophyll and carotenoid contents were measured via the method of Wellburn and Lichtenthaler,and the photosynthetic rate and chlorophyll fluorescence parameters were measured using portable photosynthesis system.Through comparison of above measured indices between P88S/0389 and its parents,the photosynthetic characteristics were obtained.[Result] The pigment content of P88S/0389 lied between their parents,and detailedly was closer to its female parent at earlier growth stage and to its male parent at latter stage,respectively.The Pn,Fv/Fo,Fv/Fm and qP of the flag leaf of P88S/0389 were all higher than those of its parents,while the degradation rates of these parameters were slower than those of its parents.The qN of flag leaf was lower than those of its parents,and the increasing rate of qN of P88S/0389 was also slower than those of its parents,indicating the great heterosis of P88S/0389 to its parents in photosynthetic capacity.The data show that P88S/0389 is endowed with stronger capacities in light energy absorption,transformation and carbon fixation compared to its parents.[Conclusion] The light energy absorbed by hybrid mainly contributes to photochemical reaction,and little for heat dissipation.For ensuring high yield,the rice cultivars selected for production should synchronously possess higher photosynthetic rate and longer high value duration. 相似文献
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超级稻P88S/0389光合特性初探(英文) 总被引：2，自引：0，他引：2
[Objective] The aim of this study is to understand the high yield often photosynthetic mechanism of super hybrid rice P88S/0389.[Method] Chlorophyll and carotenoid contents were measured via the method of Wellburn and Lichtenthaler,and the photosynthetic rate and chlorophyll fluorescence parameters were measured using portable photosynthesis system.Through comparison of above measured indices between P88S/0389 and its parents,the photosynthetic characteristics were obtained.[Result] The pigment content of P88S/0389 lied between their parents,and detailedly was closer to its female parent at earlier growth stage and to its male parent at latter stage,respectively.The Pn,Fv/Fo,Fv/Fm and qP of the flag leaf of P88S/0389 were all higher than those of its parents,while the degradation rates of these parameters were slower than those of its parents.The qN of flag leaf was lower than those of its parents,and the increasing rate of qN of P88S/0389 was also slower than those of its parents,indicating the great heterosis of P88S/0389 to its parents in photosynthetic capacity.The data show that P88S/0389 is endowed with stronger capacities in light energy absorption,transformation and carbon fixation compared to its parents.[Conclusion] The light energy absorbed by hybrid mainly contributes to photochemical reaction,and little for heat dissipation.For ensuring high yield,the rice cultivars selected for production should synchronously possess higher photosynthetic rate and longer high value duration. 相似文献
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燕辉 《中国农业科技导报》2023,25(1):58-64
为探明油菜幼苗叶绿素荧光响应低温胁迫的生理机制,将油菜幼苗进行低温胁迫处理,研究低温胁迫处理下油菜幼苗叶片的叶绿素荧光参数及其光响应曲线的变化特征。结果表明,胁迫处理后油菜幼苗叶片的最大光化学效率(Fv/Fm)与激发能捕获效率(F’v/F’m)较对照显著降低;实际光化学效率(F’q/F’m)在低温胁迫5～10 d后较对照显著降低,光化学淬灭(F’q/F’v)在低温处理1、3和10 d后较对照无显著变化,由此表明,低温胁迫使PSⅡ反应中心受损,导致反应中心捕获激发能的效率降低;此时虽然反应中心吸收的光能中用于光合电子传递的份额未发生显著变化,但光能转化效率在低温胁迫5和10 d后较对照显著降低。此外,F’q/F’v光响应曲线较对照变幅较小;但低温胁迫5～10 d后,F’v/F’m与F’q/F’m<... 相似文献
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H9亚型禽流感病毒间接免疫荧光检测方法的建立与比较 总被引：1，自引：0，他引：1
将禽流感病毒(AIV)接种犬肾上皮细胞(MDCK),24h后以鸡抗禽流感抗体和兔抗鸡荧光抗体进行间接免疫荧光试验(IFA),结果于细胞核、细胞质内观察到特异性的荧光。利用96孔细胞板培养MDCK细胞,以间接免疫荧光作为判定指标对禽流感病毒进行毒力测定,并与禽流感病毒通用荧光反转录-聚合酶链反应(荧光RT-PCR)检测方法进行比较。结果表明间接IFA、荧光RT-PCR对同一样品检测的最高稀释度分别为10-5.25TCID50和10-6,Ct<30,两种方法对禽流感病毒的检测敏感性均很高。但由于荧光RT-PCR仅对抽提的RNA进行检测,而间接IFA是对禽流感活病毒进行检测,因此在临床样品检测和动物产品检疫中间接IFA更具准确性和实用性。 相似文献