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1.
The in vivo pediculicidal effectiveness of 1% and 2% formulations of tea tree (Melaleuca alternifolia) oil (TTO) against sheep chewing lice (Bovicola ovis) was tested in two pen studies. Immersion dipping of sheep shorn two weeks before treatment in both 1% and 2% formulations reduced lice to non detectable levels. No lice were found on any of the treated sheep despite careful inspection of at least 40 fleece partings per animal at 2, 6, 12 and 20 weeks after treatment. In the untreated sheep louse numbers increased from a mean (±SE) of 2.4 (±0.7) per 10cm fleece part at 2 weeks to 12.3 (±4.2) per part at 20 weeks. Treatment of sheep with 6 months wool by jetting (high pressure spraying into the fleece) reduced louse numbers by 94% in comparison to controls at two weeks after treatment with both 1% and 2% TTO formulations. At 6 and 12 weeks after treatment reductions were 94% and 91% respectively with the 1% formulation and 78% and 84% respectively with the 2% formulation. TTO treatment also appeared to reduce wool damage in infested sheep. Laboratory studies indicated that tea tree oil 'stripped' from solution with a progressive reduction in concentration as well as volume as more wool was dipped, indicating that reinforcement of active ingredient would be required to maintain effectiveness when large numbers of sheep are treated. The results of these studies suggest significant potential for the development of ovine lousicides incorporating TTO.  相似文献   

2.
The sampling distribution of Bovicola ovis (Schrank) on sheep was examined in two flocks, one with a light and one with a heavy infestation of lice. The derived distributions were used to calculate the sensitivity of detecting lice on individual sheep and in flocks by fleece parting regimes that varied in number of parts per animal and number of sheep per flock, different scenarios of flock sizes, proportion infested and louse density were examined. Lice were aggregated among fleece partings in the heavily infested flock and described by a negative binomial distribution with k values between 0.3 and 1.92. The distribution was indistinguishable from Poisson in the lightly infested flock. The assumed distribution had little effect on sensitivity, except when only one fleece part per animal was examined. On individual sheep where louse density was 0.5 per 10 cm part or greater, there were only marginal gains from inspecting more than 10 parts per animal. Increasing the number of sheep inspected always increased sensitivity more than increasing number of parts per sheep by an equivalent amount. This advantage was greatest in situations where a low proportion of sheep in the flock were infested with a high density of lice, and less where a low proportion of sheep were infested with a low density of lice, or a high proportion of sheep were infested with a high density of lice.  相似文献   

3.
The spatial distribution of chewing lice (Bovicola ovis) on their hosts was examined in Polypay and Columbia ewes initially artificially infested on the midside or the neck. Densities of lice were determined at 69 body sites in eight body regions at approximately monthly intervals for 2 years. In the second year, half of the ewes were mated and lice were counted at 26 body sites on the resulting lambs. Polypay ewes had higher densities of lice than Columbias at most inspections but there was little effect of infestation point or mating on either numbers or the distribution of lice. During periods of high louse numbers densities were generally greatest on the sides or the back. Densities on the head were also high at times and peaked later than overall louse densities. Shearing markedly reduced density but increased the proportion of lice found on the neck, belly and lowleg sites. The distribution of lice on the lambs was similar to that on the ewes except that fewer lice were found on the head. Comparisons of lice per part with the numbers of lice extracted from clipped patches indicated that a sheep with wool bearing area of 1 m2 and a mean count of one louse per 10 cm fleece parting carried approximately 2000 lice. At most times of the year inspections for sheep lice should be concentrated on the sides and back, but in recently shorn sheep greater attention should be paid to the lower neck and ventral regions. Implications of the observed distributions of lice for the efficacy of chemical treatments are discussed.  相似文献   

4.
An investigation of sheep flocks in the main sheep raising areas of New South Wales showed that the itchmite Psorergates ovis was frequently associated with fleece derangement. In 26 of the 41 flocks examined, P. ovis was the only ectoparasite detected. P. ovis and the sheep body louse Damalinia ovis, were found in 5 flocks. No external parasites were found on sheep examined from the 10 remaining flocks. The type of fleece derangement most frequently recorded was rubbing which in some cases was combined with areas of chewed fleece. Among flocks, there were positive relations between the prevalence of fleece derangement and prevalence of itchmite or scurf and between itchmite count and mean scurf score. Within flocks, itchmite infested sheep or sheep with scurf had higher prevalences of fleece derangement than sheep on which no mites or no scurf were found. Itchmite infested sheep had a higher prevalence of scurf than those with no detectable mite infestation. There were no significant differences in itchmite populations or fleece derangement between untreated flocks and flocks treated with synthetic pyrethroids, organophosphates or arsenic and rotenone.  相似文献   

5.
Polymer matrix ear tags containing 13.7% w/w tetrachlorvinphos or 8.5% w/w cypermethrin were applied to Merino wethers infested with lice and carrying two months wool. The cypermethrin tags reduced louse numbers by a maximum of 89% in comparison to controls at 16 weeks after treatment and by 85% at the conclusion of the experiment 38 weeks after application. Lice were not eradicated from any sheep. The number of lice counted on sheep treated with tetrachlorvinphos-impregnated tags was not significantly different from controls.  相似文献   

6.
Knowledge of the presence or absence of lice in a flock of sheep enables wool growers to make informed decisions as to the need for insecticidal treatments. However, with inapparent infestations, traditional methods of detection are not sufficiently sensitive and, as a consequence, flocks may be left untreated. Conversely, the routine application of insecticide to sheep with no sign of infestation is an unnecessary cost. The sensitivity of 3 procedures for detecting lice was evaluated in 68 mobs of sheep from 50 farms. In 24 mobs of sheep known to be lightly infested, lice were detected in 17% by either parting the fleece of 10 sheep or by the lamp test in which 8 g samples of shorn wool from 30 randomly selected fleeces were placed under lamps for 10 min to repel the lice. Twenty of 23 mobs (87%) were found to be infested by the table locks test in which a 30 g sample of locks wool was dissolved in 10% sodium hydroxide and the filtered residue examined with x 40 magnification. A screening test, in which either fleeces on 5 sheep were examined by fleece parting or lice were repelled from 30 shorn fleeces for 5 minutes, detected about 60% of lightly infested mobs. When this was followed by the table locks test 91% of lightly infested mobs were detected. Conducting any one of the tests on more than one mob, and in large mobs testing more frequently, increases the sensitivity of detection of lice within the whole flock.  相似文献   

7.
Information provided by wool growers in Queensland, Australia between 1995 and 1997 was used to assess the prevalence and spatial distribution of louse (Bovicola ovis) infestation in sheep flocks. The estimated prevalence of louse-infested flocks was 40% (95% confidence interval, 35-46%). Although the prevalence of infestation was higher in western regions (41-50%) compared to the south region of Queensland (31%), the difference was not statistically significant (P > 0.05). Significant (P = 0.02) clustering of infested flocks was detected in the south region where two foci were apparent. We conclude that Queensland sheep flocks have a moderate prevalence of louse infestation, and that clustering of infestation is not strong. The control of lice is an industry-wide issue that needs to be addressed by most wool growers in Queensland.  相似文献   

8.
Sheep of two bloodlines of Merino were artificially infested with equal numbers of lice (Bovicola ovis) and the resulting louse populations were monitored over the following 20 months. The sheep were shorn 6 and 17 months after infestation and, for analysis, the louse counts considered in 3 years separated by shearings. Nematode faecal egg counts (FECs) were assessed on faecal samples collected on five occasions, three times following natural challenge and twice after artificial challenge with 40,000 trichostrongyloid larvae (84% Trichostrongylus vitrinus). In addition, blood samples were collected and measured for B. ovis-specific immunoglobulins (predominantly IgG), B. ovis-specific IgE and serum total IgE. Bloodlines differed significantly in the size of louse populations at the end of year 2, FEC after both natural and artificial challenge and in serum levels of all three antibodies (p<0.05). There were also large variations in louse counts and FEC among sheep within bloodlines. Louse counts at inspections after louse populations had been allowed to build up were highly repeatable, both between and within years. However, correlations with counts at inspections soon after initial infestation and following shearing were lower. FEC after natural challenge was correlated with louse counts in year 2 (r=0.45, p<0.01) and year 3 (r=0.38, p<0.05), but the correlation with counts in year 1 was not significant (r=0.25, p>0.05). FEC following artificial challenge was significantly correlated with louse counts in year 3 (r=0.36, p<0.05), but not in year 2 (r=0.25, p>0.05) or year 1 (r=0.04, p>0.05). Louse counts in the 3 years were significantly correlated with anti-B. ovis antibody concentration (r=0.60, 0.48, 0.36), but not with levels of either anti-B. ovis or total serum IgE.These results suggest that sheep with greater resistance to gastrointestinal parasites also tend to be less susceptible to lice. Whether this is due to interaction of the effects of the parasites or to correlation in underlying resistance mechanisms requires clarification.  相似文献   

9.
1. This paper describes the influence of some epidemiological determinants on the prevalence of louse infestation on layers in Faisalabad (Pakistan). 2. Seventy layer farms were screened to determine the prevalence of louse infestation and its determinants including age of the bird, availability of space per bird and frequency of litter change. Prevalence of Lipeurus caponis was highest (53.2%), followed by Menacanthus stramineus (22.16%), Goniodes dissimilis (12.37%) and Goniodes gallinae (12.37%). 3. Older birds (36 to 74 weeks of age) were found with a significantly higher prevalence of louse infestation than younger birds. 4. Month-wise prevalence of louse infestation was higher during the summer months (from April to August) and at older age. 5. Birds kept at floor area allowances ranging from 0.43 to 0.55 m(2) were highly infested with lice as compared to those provided with a space of 0.61 m(2) or higher per bird. 6. Litter change frequency after every 8 weeks resulted in a reduced chance of louse infestation as compared to less frequent litter changing practice. 7. It was concluded from the study that the louse infestation is a menace for the poultry production industry in Pakistan and age, space per bird and litter change frequency are the influencing determinants. 8. Further research on the chemotherapeutic trials and resistance in poultry should be continued for an effective lice control program.  相似文献   

10.
The possibility that Damalinia ovis ingests intact epidermis could explain economically important irritation behaviour by infested sheep. To test this, feeding lice were observed on sheep by hand lens and macrophotography and on light and scanning electron microscope preparations. Aspects of feeding behaviour were described, particularly an epidermal ingestion posture. Louse ingesta were examined in paraffin and frozen sections. Lipid-covered stratum corneum squames were identified in crop, midgut, rectum and faeces. Nucleated keratinocytes from inner epidermal strata were not seen, neither were wool fibres. Vertical frozen sheep skin sections, with feeding lice cryofixed by liquid nitrogen, showed mandibles engaged in the outer stratum corneum of the epidermis. The epidermal origin of some ingesta was confirmed by confirming lice over sheep whose skin was stained with Oil Red O, whose colour was seen in crop, midgut, rectum and faeces of harvested lice. Lipase was found in louse midgut. It was concluded that sebaceous secretions may form an important component of the D. ovis diet. The relative contribution from loose scurf or from stratum corneum squames was not determined. Mechanical stimuli by feeding lice did not seem to be the source of host irritation. The role of sensitising agents in louse brei filtrates was investigated with inconclusive results. Intradermal injections of louse faeces did not elicit a demonstrable response.  相似文献   

11.
The comparative efficacy of 13 of the sheep dips currently registered in New Zealand was investigated using sheep infested with the louse Bovicola ovis and carrying wool which was about 10 cm long at the shoulder. With the exception of one synthetic pyrethroid pour-on formulation, all products were able to effect a significant reduction in louse populations, relative to untreated controls, for 37 days after treatment. Only four products proved capable of eradicating lice and preventing their re-establishment up to 37 days after treatment. Variations in manufacturers' recommendations relating to the length of wool at dipping, and mode of application of dips are discussed in relation to the results.  相似文献   

12.
The eradication of lice from Merino sheep with wool up to 65 mm long was achieved by spraying insecticide onto the tip of wool over the sides and back. The insecticides used were cyhalothrin and diazinon in small volumes and at high concentration. When infested sheep were sprayed with 100 ml of cyhalothrin 1,500 ppm or diazinon 36,000 ppm, no live lice were seen 23 days after treatment. When treated sheep were challenged with live lice 100 days after treatment an infestation did not establish. Cyhalothrin at a lower concentration of 1,000 ppm gave excellent control but at 500 ppm only fair control was obtained. Diazinon at 24,000 ppm gave probable eradication and at 12,000 ppm excellent control. If a practical method of applying the insecticide to the tip of wool can be developed, then this technique should provide an effective means of eradicating lice in sheep with long wool. This would greatly assist the sheep industry in controlling and eradicating sheep lice.  相似文献   

13.
The application of polymer matrix ear tags impregnated with 8.5% w/w cypermethrin to 6 wethers following shearing reduced lice to non-detectable levels on 4 of them at 29 weeks after tagging. At the conclusion of the study at 45 weeks the mean count of lice on tagged wethers was 3 per sheep compared to 158 on untreated wethers. In a second experiment, 6 non-infected sheep were treated at shearing with cypermethrin tags, 6 with 25 g/l cypermethrin backline formulation, 6 with tags plus backline and 6 were left untreated. Each group was exposed to 6 sheep with moderate to heavy infestations of lice. Compared to controls, all treatments delayed infestation, but cypermethrin tags gave no longer protection than backline treatment. All sheep were infested by 17 weeks after commencement of the study. At 45 weeks mean counts of lice were 38, 27, 20 and 74 respectively for sheep treated with tags, backline formulation, backline plus tags and untreated. Possible reasons for the better effect observed from applying tags to infested sheep than to sheep which were not infected at application but which were subsequently exposed to infested sheep are discussed.  相似文献   

14.
The comparative efficacy of 13 of the sheep dips currently registered in New Zealand was investigated using sheep infested with the louse Bovicola ovis and carrying wool which was about 10 cm long at the shoulder.

With the exception of one synthetic pyrethroid “pour-on” formulation, all products were able to effect a significant reduction in louse populations, relative to untreated controls, for 37 days after treatment. Only four products proved capable of “eradicating” lice and preventing their re-establishment up to 37 days after treatment.

Variations in manufacturers' recommendations relating to the length of wool at dipping, and mode of application of dips are discussed in relation to the results.  相似文献   

15.
SUMMARY An investigation of sheep flocks with unknown histories of fleece derangement, selected at random from the main sheep raising areas of New South Wales, showed that Psorergates ovis was present in 53 of the 177 flocks surveyed and In 162 of the 3495 sheep examined. Of the single causes of fleece derangement, the most prevalent was grass seed contamination (15.7%) followed by Infestation with sheep body lice, Bovicola ovis, (14.7%) and Infestation with Itch mite (7.8%). The prevalence of fleece derangement was significantly higher on sheep In which lice were the only ectoparasites found than on those In which Itch mites were the only ectoparasites present. The greatest prevalence of P ovis was In flocks In the Young, Yass, Mudgee and Tenterfield districts. In most affected flocks, more than one potential cause of fleece derangement was present. Itch mite Infestation was more prevalent in flocks that received no treatment for ectoparasites than in those that had been treated in the year preceding the survey. Our results did not support a commonly held belief among farmers that arsenical compounds control Infestations of Itch mites more effectively than other types of pesticides. There was no significant difference in prevalence of fleece derangement between flocks treated with different pesticide groups and those that received no treatment, but mean fleece derangement score was significantly lower in sheep treated with organophosphates than in untreated sheep. Infestations of lice were significantly more prevalent in sheep from flocks treated with synthetic pyrethroids than from those treated with organophosphates or those that received no treatment.  相似文献   

16.
Transmission of sheep lice is thought to occur mainly by sheep to sheep contact although the possibility of other sources of infestation is often suggested. This study investigated the period of survival of Bovicola ovis after removal from sheep under varying conditions and assessed the likelihood of new infestations arising from contaminated facilities, wool caught on fences and shearers' footwear.In laboratory studies with lice held away from sheep at 4, 20, 25 and 36.5 degrees C, adults and nymphs survived longest at 25 degrees C (LT90 of 11.7 and 24.1 days for adults and large nymphs, respectively). Nymphs survived longer than adults and lice provided with raw wool survived longer than lice provided with wool that had been degreased. Nymphal lice survived for up to 29 days on unscoured wool at 36.5 degrees C, but the LT50 was less than 9 days in most experiments. In shearing sheds in winter and early spring lice survived for up to 14 and 16 days, respectively. These periods of survival are considerably longer than previously indicated for B. ovis. Most lice dropped out of wool staples attached to a fence within 1 h and only two of a total of 225 lice were still present after 24 h, suggesting that sheep are unlikely to become infested from wool caught on fences. Adult and nymphal lice readily transferred to shearers' moccasins and survived there for up to 10 days, indicating that transmission of lice on the footwear of shearers or other sheep handlers may be a cause of new infestations. Microwaving each moccasin for 5 min killed all lice and may provide a simple method of reducing the likelihood of transmission of B. ovis between properties.  相似文献   

17.
Summary The relative efficacy of 6 shower dip chemicals most frequently used for the treatment of sheep lice (Bovicola ovis) in Western Australia was examined. Groups of 20 sheep infested with lice were treated with products containing either alphamethrin, cyhalothrin, diazinon or diazinon plus piperonyl butoxide and rotenone, formulated as emulsifiable concentrates, and with products containing either coumaphos or magnesium fluorosilicate, formulated as wettable powders. All treatments were applied through a shower dip (Sunbeam model SSD). Inspections for lice were conducted until 9 months after dipping. No lice were found on sheep treated with the 4 emulsifiable concentrate products. In contrast, treatment with the wettable powders, which contained either coumaphos or magnesium fluorosilicate as the active ingredient, did not eradicate the lice infestations. The degree to which the fleece was wetted was assessed 20 minutes after dipping and showed that the wettable powder dips penetrated the fleece less than the emulsifiable concentrate dips. Less fluid was retained by wool staples in an in-vitro test when dip wash was made with the wettable powders. It was concluded that the degree of wetting attained at dipping was an important factor in achieving eradication of sheep lice.  相似文献   

18.
Beef calves from 2 sources entering southern Alberta feedlots in the winters of 1997-98 and 1998-99, were surveyed for the presence of lice. A random sample of multiple source (MS), that is, auction market-derived, calves entering commercial feedlots and single source (SS) calves entering a backgrounding feedlot were examined for the presence of lice at entry to the feedlot. A standardized examination, which involved hair-part examination of 8 louse predilection sites, was conducted on each selected calf to determine prevalence and intensity of infestation. The long-nosed sucking louse, Linognathus vituli, was the most commonly encountered species. This species infested from 57.8% to 95.6% of the calves selected from both MS and SS calves during both winters. Louse index values, indicating intensity of infestation, for L. vituli ranged from 1 to 243 lice per animal. The chewing louse, Bovicola bovis, was present on MS and SS calves only in the winter of 1998-99. The louse index values for B. bovis ranged from 1 to 230 lice per animal. Mixed infestations of the L. vituli and B. bovis were common. The little blue cattle louse, Solenopotes capillatus, was present only on the SS calves in the winter of 1997-98. The short-nosed sucking louse, Haematopinus eurysternus, was present at very low intensities, 1-2 lice per animal, on 2.6% to 4.4% of the MS calves during both winters. Comparison of results from the current study with published literature suggests that efforts to determine the economic impact of louse infestations are confounded by the lack of a uniform method to assess louse population levels.  相似文献   

19.
Infestation of sheep with the biting louse Damalinia ovis is a potentially serious problem which can effect the quality and quantity of wool produced and may reduce weight gains. In this trial treated sheep produced 34 per cent more wool than louse-infested controls and the wool from the treated sheep was of a better quality. The mean liveweight gain in the treated groups was 18 per cent more than that in the untreated group. Treatment with a pour-on formulation of propetamphos at 25 to 50 mg kg-1 gave more than 99 per cent control of lice and protected against reinfestation for four months.  相似文献   

20.
Objective To compare the wettability and efficacy of diazinon dip wash made with and without the addition of zinc sulphate.
Design Field experiments using a shower and a plunge dip complemented by in-vitro wettability experiments.
Procedure A flock of infested sheep was divided into groups and treated in a shower dip with clear or cloudy dam water plus up to 1.5% zinc sulphate. Another infested line of sheep was treated using a plunge dip with nil or 1% zinc sulphate. In both experiments, wetting was assessed after dipping and louse counts were conducted for 9 months after treatment. Five in-vitro experiments compared the wettability of dip wash containing diazinon with up to 1.5% zinc sulphate added.
Results In the shower dipping experiment, live lice were found at 1 month after dipping in the cloudy water groups with 0.75%, 1.0% and 1.5% zinc sulphate and at 2 months in the 0.75% zinc sulphate group. No lice were found at subsequent inspections or at any time in the groups that were plunge dipped. Zinc sulphate decreased the amount of dip wash retained by wool staples in all in-vitro experiments (P < 0.05).
Conclusion Zinc sulphate should be considered as a risk factor that could cause failure to eradicate a lice infestation. The risk can be overcome by ensuring that all sheep are saturated at dipping and that the dip wash, and any holding tanks, are agitated throughout the dipping event.  相似文献   

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