共查询到18条相似文献，搜索用时 468 毫秒
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为了解沙棘种质资源的遗传多样性和品种间的亲缘关系,以24个大果沙棘品种为材料,利用随机扩增多态性DNA(RAPD)方法进行沙棘的遗传多样性分析。从120个随机引物中筛选出多态性丰富的18个引物用于扩增反应,在24个大果沙棘品种中检测到171个RAPD位点,其中多态性DNA带112条,多态位点百分比为65.5%,表明大果沙棘品种具有丰富的遗传多样性;在RAPD分析的基础上,对大果沙棘品种进行了聚类分析,结果表明,以相似系数0.58处作为分界,可将供试的24份大果沙棘种质分为4类。 相似文献
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核桃早实性相关性状的SCAR标记 总被引：4，自引：0，他引：4
用RAPD技术对新疆核桃早实特性的分子标记进行了研究。用180个10-mer随机引物分别扩增早实和晚实近等基因池DNA筛选出5个多态性引物,结果只有引物OPG15(5′-ACTGGGACTC-3′)扩增得到1条约710bp的早实核桃特异片段。对该片段进行克隆和序列分析,并根据序列分析结果将该RAPD标记转化为重复性和特异性更好的SCAR标记。研究设计出了1对早实核桃特异SCAR引物P1(5'-ACTGGGACTCCAATTGTATC-3')和P2(5'-ACTGGGACTCTCAACTAT-3'),用这对特异引物对14份材料进行PCR扩增,结果所有晚实核桃材料无任何扩增,但早实材料均扩增出了预期大小759bp的特异带。 相似文献
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以罗汉果雌雄单株各30份为试材,采用随机引物筛选法,筛选与罗汉果性别相关的RAPD标记,并根据特异片段的测序结果,将RAPD标记转化为特异性和稳定性更好的SCAR标记,以期为罗汉果的性别早期鉴定和分子辅助育种研究提供参考依据。结果表明:在330条随机引物中获得了分别与罗汉果雌性和雄性相关的RAPD引物S2124和S343。根据特异序列测序结果,对雌性特异带S2124-1K和雄性特异带S343-1.4K各自设计并合成了2对SCAR引物,经验证,SCAR引物SC2124-1K-14在62℃和SC2124-1K-8在56℃均可扩增出1 019bp的雌性特异带,SC343-1.4K-12在64℃和SC343-1.4K-4在56℃均可扩增出1 373bp的雄性特异带。这些特异的SCAR标记具有较好的应用价值,可为罗汉果的性别早期鉴定提供分子依据。 相似文献
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13个阿月浑子品种的RAPD分析 总被引：2，自引：1，他引：1
为探明引进的阿月浑子品种间的亲缘关系,采用RAPD 标记技术对引入的12 个国外品种和‘新疆优株’进行了分析。用筛选的21 条10 bp 随机引物进行PCR 扩增可扩增出137 个位点,其中多态位点122 个,多态位点比率89.05%;品种间遗传距离在0. 2015 ~ 0.5163 之间,表明各品种间存在一定的遗传差异。UPGMA 聚类分析表明,13 个阿月浑子品种在遗传距离0.40 处可划分为3 个类群,第1 类包括 ‘Kerman’、‘Larnaka’、‘M-38’、‘M-P3’、‘Ashoury’、‘N’、‘Joley’、‘Aegina’、‘Avidon’、‘B’和‘Peter’11 个品种;第2 类为‘Xinjiang select tree’品种;第3 类为‘Mateur’品种。 相似文献
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燕山板栗种质资源遗传多样性的RAPD分析 总被引：4，自引：0，他引：4
为研究燕山板栗的遗传多样性,采用随机扩增多态DNA(randomamplifiedpolymorphicDNA,RAPD)技术对36份燕山板栗种质进行了分析。分析了燕山板栗的遗传丰富度,并对包括36个燕山板栗品种和8份外来板栗品种在内的44份板栗种质进行聚类分析。结果表明,RAPD能有效地区分品种间的差异,用16个随机引物经PCR扩增共得到132个片段,其中有83个多态性片段,占62.9%;不同遗传位点之间遗传多样度最大可达0.444,最小值为0.096,平均多样度为0.187;UPGMA法聚类,将44份板栗种质聚成4个大的类群,36份燕山板栗可分为3个大的类群,外来种质聚为一类。燕山板栗明显不同于外来品种。在RAPD图谱中,找到了19个品种(类型)的特异性标记和标记组合,可作为品种(类型)分子鉴别的依据。 相似文献
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15个樱桃品种的RAPD分析 总被引：20，自引：4，他引：20
利用RAPD技术,用104条随机引物对甜樱桃的14个品种和中国樱桃的1个品种进行遗传多样性分析,其中有14条引物的多态性较好。用任意一条能出现扩增带的引物,能明显区分开中国樱桃和甜樱桃,RAPD标记能够准确地进行种间的区分;而用一个引物或两个引物的组合只能鉴定出甜樱桃的一个品种。聚类结果显示,中国樱桃和甜樱桃的遗传距离最远;黄色果肉的养老和其他红色果肉的品种遗传距离较远。RAPD分析基本能够反映甜樱桃品种间的遗传多样性,但效果不理想,鉴定品种较为困难。 相似文献
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桃品种以重阳红(花粉不育) ×大久保(花粉可育) 的52株F1代群体为试材, 应用RAPD技术, 结合集群分组分析法(Bulked Segregate Analysis, BSA) 构建花粉可育/不育基因池, 利用180个随机引物, 筛选在花粉可育基因池中稳定扩增的一个RAPD标记OPW03-900。经过重复性验证和群体单株验证, 该标记仅在花粉可育个体(重组型除去) 中出现, 与桃花粉可育/不育位点的连锁距离为5.80cM。将该特异性片段回收、克隆、测序, 并设计一对特异SCAR引物, 再对F1代个体进行PCR扩增, 发现该特异带的位点及重组型个体的数目与RAPD扩增结果一致, 片段大小为906 bp, 命名为SCW03-906,表明RAPD标记已成功转化为与桃花粉可育基因连锁的SCAR标记。该序列已被GenBank收录, 登录号为DQ659676。 相似文献
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Yoshimi Yonemoto Abul Kashem Chowdhury Hidenori Kato Mustad Malid Macha Hitoshi Okuda 《Scientia Horticulturae》2007
Floral morphology, random amplified polymorphic DNA (RAPD), and amplified fragment length polymorphism (AFLP) were used to characterize and verify genetic diversity within a white sapote cultivar collection and to develop molecular markers for germplasm identification. On the basis of floral morphology, the cultivars were classified into three types: type I included 23 cultivars with large ovaries and small anthers; type II included 13 cultivars with small ovaries and large anthers; and type III included one cultivar, named ‘Maltby’, with a large ovary and large anthers. DNA was isolated from 39 cultivars of white sapote and subjected to RAPD and AFLP analysis using 24 and 7 primers, respectively. One hundred and sixty-eight RAPD and 286 AFLP bands were used to assess genetic characterization among white sapote. Sixty percent of the RAPD and 77% of the AFLP amplification products were polymorphic among accessions. RAPD or AFLP markers differentiated all white sapote cultivars effectively. Moreover, each flower type was characterized as specially associated with two RAPD bands. UPGMA dendrograms based on RAPD and AFLP data, showed the majority of the cultivars from flower type I and flower type II clustering together. Finally 101 RAPD markers and 220 AFLP markers were used to construct a neighbor-joining dendrogram. This showed that the 37 cultivars could be classified into six distinct clusters, between which the similarity coefficient was as low as 0.00–0.55, even though the cultivars were morphologically very similar. The remaining two cultivars namely ‘Smathers’ and ‘Maltby’ were found genetically very distant from the other cultivars in RAPD, AFLP or combined RAPD and AFLP based dendrograms. The results suggested that the level of genetic variation among white sapote cultivars is diverse and the morphological and molecular data may lead to representation of the cultivar relationships as well as flower type discrimination. 相似文献
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The objectives of this study were to investigate genetic variation and relationships between Indonesia-, Australian- and European-based cultivars and to evaluate variation within Indonesia cultivars as all cultivars are open-pollinated. Eight cauliflower cultivars collected from three production regions in Indonesia and four F1 hybrids cultivars grown in Australia were evaluated using RAPD and ISSR markers. DNA polymorphisms generated from 10 polymorphic RAPD primers were used to construct a dendogram using the unweighted pair-group method with arithmetic averages (UPGMA) and to generate a fingerprinting key. ISSR marker analysis using 14 primers were attempted but DNA polymorphisms could not be clearly identified. The RAPD technique indicated that variation occurred both within and between Indonesian cultivars. Comparison between Indonesian-, Australian- and European-based cultivars showed that Indonesian cultivars have unique genotypes and would be good sources of genes for future crop improvement. 相似文献
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M. Sangeeta Kutty Lalitha Anand 《The Journal of Horticultural Science and Biotechnology》2013,88(4):774-778
SummaryRandomly amplified polymorphic DNA (RAPD) markers were used to estimate genetic diversity among 24 cultivars of short-day onions. Total genomic DNA was extracted and subjected to RAPD analysis using 90 arbitrary 10-mer primers. Of these, 15 primers were selected which yielded 137 bands, 91.24% of which were polymorphic. None of the primers produced a unique banding pattern for each cultivar. RAPD data were used to calculate a Squared-Euclidian Distance matrix which revealed a minimum genetic distance between cultivars ‘AFLR-722’ and ‘PBR-140’, and a maximum genetic distance between cultivars ‘PBR-139’ and ‘A.Kalyan’, and ‘MS-48’ and ‘A.Kalyan’. Based on the distance matrix, cluster analysis was done using a minimum variance algorithm.The dendrogram thus generated, based on Ward’s method, grouped the 24 onion cultivars into two major clusters. The first cluster consisted of cultivars from the northern region, and the second of cultivars from the southern region of India. The present study shows that there is high diversity among the onion cultivars selected and indicates the potential of RAPD markers for identification and maintenance of onion germplasm for crop improvement purposes. 相似文献
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利用RAPD标记研究部分葡萄品种的亲缘关系 总被引：4，自引：1，他引：3
对47个葡萄品种进行RAPD分析。从100条随机引物中筛选出30个多态性引物用于DNA扩增,共扩出216条DNA带,121条具有多态性,多态性比例为56.01%。通过聚类分析结果表明,供试品种分成5大类,巨峰、红瑞宝、龙宝等巨峰系品种聚为一类。其中龙宝和红瑞宝优先聚在一起。表明葡萄品种间的遗传多样性有明显差异,即使父母本相同的品种,利用RAPD技术也可以分开。聚类图显示,品种间遗传一致度与品种的遗传背景有关,如高妻与红井川,红瑞宝与龙宝优先聚在一起;有的品种虽属同一杂交组合却未能表现最大遗传一致度,如京玉与奥古斯特,在聚类图中处于相邻的位置,说明无性繁殖的葡萄在双亲基因型杂合条件下,有性杂交通过基因重组,杂种后代也可呈现出有较大遗传差异。 相似文献
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杧果种质遗传多样性的RAPD分析 总被引：15，自引：2，他引：15
对杧果不同生态型、不同胚性、不同果形与果色的38份品种和1个近缘种—扁桃进行了RAPD分析,19个引物在39份种质中共扩增出223条带,其中多态性带为196条,多态性带的百分率为87.89%,表明品种间存在着广泛的遗传基础。利用UPGMA进行聚类分析,在相似性系数0.755的水平上将38个品种分成3组,该结果与传统上以胚型为依据进行品种类群划分比较吻合。发现了多个与胚性密切相关和1个与果皮颜色密切相关的RAPD标记,并就部分品种的系谱关系、胚性和果皮颜色的遗传进行了探讨。 相似文献
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23个石榴基因型遗传多样性的SRAP分析 总被引：13，自引：1，他引：12
以23个石榴基因型为试材,利用7对SRAP引物进行PCR扩增,共扩增出1380个条带,其中多态性条带662个,多态率为48%。应用DPS分析软件构建UPGMA聚类图,23个基因型遗传距离在0.0769～0.4131。在遗传距离0.33处,可将石榴基因型分为A、B、C、D和E5个类群。4种单瓣白花基因型都聚类在A组;B组都是单瓣红花、味甜的鲜食品种;C组中鲜食品种果皮都不着色;4种不同花色或叶型的单瓣、赏食兼用基因型则聚在D组;观赏型墨石榴与其它基因型显著不同,单独聚为一类(E组)。引物对组合ME4/EM4-800bp缺失条带是青皮石榴的特异缺失标记,ME4/EM4-480bp缺失条带是峄城红和河阴软籽石榴的特异缺失标记,ME2/EM3-120bp缺失条带是峄城红、白皮酸和大青皮的特异缺失标记。研究结果表明,石榴基因型有着丰富的遗传多样性,SRAP技术可有效运用于石榴基因型的遗传分析。 相似文献
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Li-Wang Liu Li-Ping Zhao Yi-Qin Gong Ming-Xia Wang Li-Ming Chen Jin-Lan Yang Yan Wang Fan-Min Yu Long-Zhi Wang 《Scientia Horticulturae》2008
Three molecular marker systems, RAPD (random amplified polymorphic DNA), ISSR (inter-simple sequence repeat) and SRAP (sequence-related amplified polymorphism), were employed for identification and genetic diversity analysis of 35 elite late-bolting radish cultivars. Detected by 35 RAPD primers, 22 ISSR primers and 17 SRAP primer combinations, the proportions of polymorphic bands were 85.44%, 85.2% and 85.41%, respectively, and the mean genetic similarity coefficients between pairs of genotypes were 0.781, 0.787 and 0.764, respectively. Each of the three molecular marker systems can identify all the cultivars. Five sets of three-RAPD primers, 3 sets of three-ISSR primers and 16 sets of three-SRAP primer combinations were able to distinguish all the cultivars. A linear relationship was observed between Resolving power (Rp) of a primer and its ability to distinguish genotypes. The 35 cultivars were clustered into three major groups based on the RAPD, ISSR and marker combination data with UPGMA, which are in high accordance with their own origins and main characteristics. The results demonstrated that these three marker systems could be useful for identification and genetic diversity analysis of radish cultivars. 相似文献