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1.
This paper reports on the effect of various micropropagation factors of Gmelina arborea Roxb. through multiple shoot induction. Factors like the source and age of explants, plant growth regulators （PGRs）, media composition, and carbon source affected multiple shoot-ing in the present study. Among all the explants used, only shoot tips derived from one, two, and three week old seedlings could form multiple shoots. Besides, the formation of multiple shoots depended on the con-centration and combination of PGRs. Among all the PGRs, BAP （6-benzylaminopurine） alone gave the highest regeneration efficiency. Simi-larly, IBA （Indole-3-butyric acid） was the most efficient PGR in inducing root formation in the microshoots. Media composition and carbon source also affected the regeneration efficiency. MS （Murashige and Skoog medium） proved to be the best media for regeneration followed by B5, SH （Schenk and Hilderbrandt medium） and WPM （Woody plant medium） in that order. Similarly, among sugars, only sucrose and glucose sup-ported induction of microshoots. Based on this study we recommend the use of glucose in place of sucrose in MS medium for maximum regenera-tion efficiency. 相似文献
2.
爬行卫矛下胚轴高频离体再生体系的建立(英文) 总被引：1，自引：0，他引：1
In this paper,a protocol for efficient shoot regeneration was successfully developed from hypocotyl explants of Euonymus fortunei var.radicans.Some factors that influenced shoot regeneration such as different combinations of plant growth regulators,types of medium and inoculation ways were studied in order to establish an efficient plant regeneration for transformation.The results showed that hypocotyl explants wero horizontally cultured on a basic medium composed of MS medium supplemented with 0.5 mg·L-1 BAP and 0.01 mg·L-1 NAA for induction and development of adventidous shoots.Ninety-four percent of regeneration frequency and 5.1 shoots per explants were obtmned after 30 days of culture.Regenerated shootsproliferated efficiently on a shoot multiplication medium consisting of MS medium containing 1.0 mg·L-1 BAP and 0.1 mg·L-1 NAA.Microshoots were rooted on a rooting medium made up of MS medium enriched with O.5 mg·L-1 IBA and O.5 mg·L-1IAA.After hardening,90% of plants were successfully established under greenhouse conditions.Histological observation revealed that shoot primordium originated from subepidermal cells of hypocotyl explants and directly developed into adventitious shoots without caHus formation. 相似文献
3.
以生长和生根兼优的杂种落叶松无性系日永8×长混18-10的嫩枝和休眠芽为试验材料,开展了嫩枝生长和芽增殖的离体组织培养技术研究,确定了两种试验材料的最佳灭菌条件及最适培养基。结果表明:15%的次氯酸钠溶液处理10 min,材料无菌存活率达到91.7%,灭菌效果最好;最适嫩枝生长培养基为B培养基;芽增殖过程中,最适腋芽萌发诱导培养基为BEMB培养基,单个嫩枝平均诱导萌发2个腋芽;无外源激素的改良BEMB培养基(硝态氮与铵态氮含量比为6:1),最适于腋芽成枝诱导,成枝率为36.5%;培养基MCM+1.5 mg·L-1 ZT +0.15 mg·L-1 KT,最适用于休眠芽不定芽诱导,不定芽诱导率为39.1%,增殖倍数为2.6。 相似文献
4.
A protocol was developed for shoot proliferation and plantlet formation of Khaya senegalensis, an important medicinal and timber plantation species introduced to Australia and southern Asia from western and central
Africa. We assessed effects of the plant growth regulators, benzyladenine, kinetin, naphthalene acetic acid and gibberellic
acid, on shoot proliferation and subsequent plantlet conversion. Shoot proliferation over four passages was higher in media
containing benzyladenine than in media containing other growth regulators, and optimal proliferation from seed of three different
sources was consistently obtained in medium containing 4.4 μM benzyladenine. Shoots from this medium were converted to plantlets
at high frequencies (76–90%) after treatment with 19.6 μM indole-3-butyric acid, and almost all plantlets were successfully
acclimatized to nursery conditions. These methods provide the means for establishing in vitro and ex vitro clone banks of
juvenile K. senegalensis trees for field selection of desired genotypes and tropical plantation establishment. 相似文献
5.
《林业研究》2016,(6)
Cinchona officinalis(Rubiaceae) is an endemic species of the Loja Valley in southern Ecuador with medicinal uses. Because of over-exploitation in the nineteenth century and more recent disturbances to its ecosystem, C. officinalis populations are threatened. Currently,natural regeneration of the populations is low, despite its high plant regeneration and seed formation capacity. In the present study, an efficient protocol for germination, shoot proliferation and plantlets regeneration was developed for this species. Phenolic content and germination rate of C.officinalis seeds were compared with a control species, C.pubescens. Nodal segments from seedlings of C. officinalis were cultured on Gamborg medium supplemented with different combinations of plant growth regulators. Because the phenol content is high in C. officinalis, the phenolic should be removed with hydrogen peroxide or water washes to stimulate germination. Shoots and callus developed from nodal segments within 45 days using most of the tested combinations of plant growth regulators. The best rates of shoot proliferation, callus formation and adventitious buds were obtained in medium supplemented with 5.0 mg L~(-1) 6-benzyl-aminopurine and 3.0 mg L~(-1) indole-3-butyric acid. 相似文献
6.
LI Yanju * YANG Zhengli SHIGERU Hisajima Institute of Agricultural Resource Division Planning Chinese Academy of Agricultural SciencesZhongguancun South St. No Beijing P.R.China Institute of Applied Biochemistry University of Tsukuba Ibaraki Japan 《中国林业科技(英文版)》2004,3(1):93-98
1INTRODUCTIONElaeagnusangusifoliaLisahighlytolerantnativewoodyspeciesinsouthernEuropeandAsia.Itmaybeusedinreforestationandsoil-waterconservationandforanexcellentwindbreaktoarrestwind(Bertrand,1985;LiShaozhong,etal,1997),materialsoffragranceandmedicineandnutritivefood(ChangZhaofeng,etal,1994;Goncharova,1997;Rasekh,1999;Ahmadiani2001;JiangFashou,etal,2002).Insomepreviousresearches,BA,2ip,KN(Bertrand,1985,Economou,1988)andBA(Iriondo1995;Mariella,1996)wereusedinshootmultiplicationofE… 相似文献
7.
以苹果抗寒矮化砧木71-3-150的茎尖为外植体,通过研究培养基中不同植物生长调节剂配比对芽苗诱导、增殖和生根的影响,筛选出适合71-3-150砧木茎尖组培快繁的培养基配方。结果表明,以MS为基本培养基,附加蔗糖30 g／L、琼脂6 g／L,适宜启动培养的植物生长调节剂为6-BA 1.0 mg／L＋NAA 0.05 mg／L;适宜增殖培养的植物生长调节剂为6-BA 1.0 mg／L＋NAA 0.05 mg／L,增殖系数可达到每4周4.75倍。以1／2MS （蔗糖15 g／L,琼脂6 g／L）为基本培养基,适宜组培苗生根的植物生长调节剂为IAA 1.0 mg／L＋IBA 0.6 mg／L,生根率达100％,每株苗平均生根9.6条,移栽成活率达90％以上。 相似文献
8.
通过对西蒙得木〔Simmondsia chinensis(Link)〕组织培养大批量繁殖技术的研究,探索基本培养基、生长调节物质及蔗糖含量等对嫩梢增殖及生根的影响,结果表明:采用春季生长的1年生实生苗以及6年生高产的优株茎节作外植体,培养于含有ZT 1～3 mg/L或BA 1～3mg/L和NAA 0.01～0.1 mg/L的MS培养基中,经30～40天,几乎所有腋芽均能长成嫩梢,切取长达4～6 cm的嫩梢,培养于含有NAA的1/2MS培养基中,经1周暗培养,1个月后生根率达93％,小植株移栽于含砂土的基质中,保持湿润,获得成功。用幼苗及优株作外植体的培养效果无显著差异,说明此法不受遗传型的限制。 相似文献
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10.
本文以温室三年生喜树腋芽为外植体,研究了不同的基本培养基和基本培养基中添加不同浓度生长调节物质(BA 或TDZ)、蔗糖、琼脂以及培养基的pH 值对喜树腋芽分化的影响。结果表明:WPM 和B5 培养基适合喜树腋芽的诱导,MS 培养基不利于喜树腋芽的诱导。适合喜树腋芽增殖和分化的最佳生长调节物质为BA 1.0mg/L 或TDZ 0.1mg/L;最佳的蔗糖浓度为30g/L;最佳的琼脂浓度为6g/L;pH5.8-6.6 的范围均适合喜树腋芽的诱导,但最佳的pH 值为5.8。图1 表5 参14。 相似文献
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12.
以西黄松成熟胚为外植体诱导不定芽,在GD 9.85～19.70 μmol·L'-1 6-BA 0.0～14.42 μmol·L-1 NAA上不定芽诱导率最高达65.8%,平均增殖率为7,最大增殖率达10;不定芽形成有2种途径,即子叶直接形成不定芽和子叶组织再分化形成不定芽;NAA不利于外植体不定芽的诱导;不定芽的生长和扩繁采用不加生长调节剂的1/2 GD和1/2 SH培养基;培养基中加入适量的活性炭有利于不定芽和根的生长.不定嫩梢在1/2 GD和1/2 SH附加不同浓度NAA和GA,3的培养基上进行生根诱导,试验结果表明:NAA对不定根的形成起主要作用,在1/2 GD 28.84 μmol·L'-1 NAA 4.17 μmol·L'-1 GA,3培养基中不定梢的生根率为16.7%.在离体培养条件下,以西黄松成熟胚为外植体获得了再生植株. 相似文献
13.
Nothofagus leoni has a restricted distribution in Chilean forests. This work determines suitable culture conditions forin vitro multiplication and rooting through shoots obtained from seedlings. Broadleaved Tree Medium was suitable for shoot multiplication.
A medium with a pulse of 0.55 μM BA in the first subculture and two subcultures on BA-free medium resulted in a multiplication
rate at day 63 of × 5.7, without callus growth or shoot neoformation. Rooted shoots of good quality (number and length of
roots without callus growth) were obtained with 1.23 μM IBA (91.4% of rooting). The first roots appeared at day 11, reaching
a higher speed of rooting at day 15. 相似文献
14.
Borkowska B 《Tree physiology》1986,1(3):303-307
Micropropagation of fruit trees results in the early onset of dormancy in the young plantlets. Prechilling of shoot cultures of sour cherry cvs Schattenmorelle and North Star at 4 degrees C for 6-8 weeks prolonged shoot extension of the plantlets but longer cold treatments adversely affected shoot growth and survival. Chilling of cultures had no affect on rhizogenesis. No significant influence of ABA, GA(3) or ABA + GA(3) was observed on root development. Gibberellic acid A(3) did not stimulate extension of the shoots, but when supplied together with ABA, it significantly decreased both shoot extension and the number of actively growing apices of plantlets of cv Schattenmorelle. The combined hormonal treatment had no effect on plantlets of the North Star cv. 相似文献
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16.
毛白杨叶片再生系统的建立 总被引：8，自引：0，他引：8
该文选用三倍体毛白杨的叶片作为外植体 ,MS作为基本培养基 ,采用不同组合的BA和NAA作为植物生长调节剂对毛白杨再生系统进行研究 .结果表明 ,在MS培养基中单独添加 1 5mg·L-1BA时 ,不定芽分化率为 90 % ,同时伴随一定程度的玻璃化现象 .单独添加 0 3mg·L-1NAA时 ,不定根产生率最高 (90 % ) ,但无不定芽生成 .采用 1 0mg·L-1BA + 0 3mg·L-1NAA组合搭配时 ,不定芽分化率最高 (95 % ) .在此基础上 ,采用M1(MS + 1 0mg·L-1BA + 0 3mg·L-1NAA)培养基 ,研究了不同着生位置的叶片、同一着生位置叶片的不同切段以及叶片在培养基上的放置方式对毛白杨叶片再生的影响 .结果表明带有叶柄的下切段比其余叶片切段不定芽再生率高 ,幼嫩叶片比老化叶片再生率高 ,叶片近轴端向下接触培养基比远轴端向下接触培养基再生率高 .叶片再生系统的建立为毛白杨的遗传转化奠定了基础 相似文献
17.
通过韦塔桉组培的全过程,对培养过程中芽丛的生长和生根情况进行探讨,得出继代增殖培养基以改良MS1 6-BA0.5mg/L NAA0.2mg/L最佳,生根培养基以改良MS2 IBA0.5mg/L NAA0.1mg/L为最佳组合。 相似文献
18.
Cinchona officinalis (Rubiaceae) is an endemic species of the Loja Valley in southern Ecuador with medicinal uses. Because of over-exploitation in the nineteenth century and more recent disturbances to its ecosystem, C. officinalis populations are threatened. Currently, natural regeneration of the populations is low, despite its high plant regeneration and seed formation capacity. In the present study, an efficient protocol for germination, shoot proliferation and plantlets regeneration was developed for this species. Phenolic content and germination rate of C. officinalis seeds were compared with a control species, C. pubescens. Nodal segments from seedlings of C. officinalis were cultured on Gamborg medium supplemented with different combinations of plant growth regulators. Because the phenol content is high in C. officinalis, the phenolic should be removed with hydrogen peroxide or water washes to stimulate germination. Shoots and callus developed from nodal segments within 45 days using most of the tested combinations of plant growth regulators. The best rates of shoot proliferation, callus formation and adventitious buds were obtained in medium supplemented with 5.0 mg L?1 6-benzyl-aminopurine and 3.0 mg L?1 indole-3-butyric acid. 相似文献
19.
20.
Axillary buds from 3-yr.-old seedlings ofCamptotheca acuminata in the greenhouse were cultured on the different basal media with different concentrations of growth regulators for shoot
regeneration for studying the effects of different basal media, different concentrations of growth regulators (BA or TDZ),
sucrose, agar and pH value on shoot regeneration from axillary bud. The results showed that B5 and WPM media were the optimal
basal media and the optimal phyotohormone was BA of 1.0 mg/L or TDZ of 0.1 mg/L; The concentrations of sucrose of 30g/L and
agar of 6g/L were most suitable for the shoot regeneration; pH value from 5.8 to 6.6 were broadly effective, but the best
at pH 5.8.
Foundation item: The research was supported by the Key Project of Chinese Ministry of Education (03061) and Supported by Application Fund
of Agricultural Research Production (03EFN216700297).
Biography: Wang Hui-mei (1973-), female, Ph.D., Lecturer in Northeast Forestry University, Harbin 150040, P.R. China.
Responsible editor: Zhu Hong 相似文献