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1.
Gert H. J. Kema 《Euphytica》1992,63(3):207-217
Summary Seven spelt wheat accessions of different origin were hybridized with the susceptible bread wheat cultivar Taichung 29 in order to study the genetics of their resistance to yellow rust (Puccinia striiformis Westend. f. sp. tritici). One Iranian and five European accessions were found to carry Yr5 of Triticum aestivum ssp. spelta var. album, whereas a factor for resistance in the Iranian accession 415 was confirmed to be genetically distinct from Yr5. The alleles for resistance in each of the accessions studied showed a monogenic dominant mode of inheritance. Twenty-eight spelt wheat accessions, including those studied for their resistance to yellow rust, were subjected to polyacrylamide-gel-electrophoresis to study variation for gliadin storage protein patterns. Thirteen distinct patterns were revealed, implying the presence of duplicates within the studied spelt wheat collection. 相似文献
2.
Genetic relationships and diversity among Tibetan wheat, common wheat and European spelt wheat revealed by RAPD markers 总被引：13，自引：0，他引：13
An endemic hexaploid wheat found in Tibet, China was taxonomically classified as a subspecies in common wheat, i.e. Triticum
aestivum ssp. tibetanum. Seven accessions of the Tibetan wheat, 22 cultivars of common wheat and 17 lines of spelt wheat were
used for RAPD analysis to study the genetic relationships of the Tibetan wheat with common wheat and spelt wheat, and to assess
the genetic diversity (GD) among and within the taxa. RAPD polymorphism was found to be much higher within spelt wheat and
the Tibetan wheat than within common wheat. The GD value between the Tibetan wheat and common wheat is lower than that between
the Tibetan wheat and spelt wheat. The result of cluster analysis showed that the 46 genotypes were distinctly classified
into two groups. Group 1 included all European spelt wheat lines, while group 2 includes all Chinese common wheat and the
Tibetan wheat accessions. However, the Tibetan wheat was substantially differentiated from Chinese common wheat at a lower
hierarchy. Our results support an earlier classification of the Tibetan wheat as a subspecies in common wheat. European spelt
wheat and the Tibetan wheat showed much higher genetic diversity than Chinese common wheat, which could be used to diversify
the genetic basis for common wheat breeding.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
3.
Summary High and low molecular weight glutenin subunit (HMW-GS and LMW-GS) compositions of 270 European spelts, 15 Iranian spelts
and 25 bread wheat cultivars were analyzed by one- and two-dimensional gel electrophoresis. The results revealed a total of
22 HMW-GS alleles (4 at Glu-A1, 11 at Glu-B1 and 7 at Glu-D1) and 32 allele combinations among the three Glu-1 loci. Two major genotypes of HMW-GS: 1, 13+16, 2+12 and 1, 6.1+22.1, 2+12 were found to occur in Central European spelt wheat
cultivars and landraces at higher frequencies of 35 and 28%, respectively. The Glu-B1 locus displayed the greatest variation and genetic diversity index (H) was 0.69 whereas Glu-A1 and Glu-D1 showed H index values of 0.26 and 0.19, respectively. The dendrogram constructed by HMW and LMW glutenin subunit bands revealed
that European spelts form a separated cluster from common wheat suggesting that spelt and common wheat form distinct groups.
In addition, all 15 Iranian spelt land variety accessions differed from European spelts and possessed similar HMW-GS alleles
to common wheat. Because of a wider polymorphism Central European spelt wheats are an important genetic reserviour for improving
common wheat quality.
Both authors contributed equally to this work 相似文献
4.
5.
In marginal, agroclimatic zones, yield is often affected by flooding, but the effect is much less for winter spelt (Triticum spelta L.) than for winter wheat (Triticum aestivum L.). This study evaluates the reaction of a wheat x spelt population (F5 RILs of Forno x Oberkulmer) to flooding stress in the early phase of germination. Lines with greater tolerance to 48 h flooding
just after imbibition showed less electrolyte leakage (r = -0.79) indicating greater membrane integrity and better survival.
Five QTL explaining 40.6% of the phenotypic variance for survival to flooding were found, and localized on the chromosomes
2B, 3B,5A, and 7S. The tolerance to 48 h flooding four days after sowing was best correlated with the mean germination time
(r = 0.8), indicating that the plants with a fast coleoptile growth during flooding are less susceptible to flooding. Ten
QTL were found for seedling growth index after flooding explaining 35.5% of the phenotypic variance. They were localized on
chromosomes 2A, 2B, 2D, 3A, 4B, 5A, 5B, 6A, and 7S. Standard varieties of spelt and wheat showed the same tolerance characteristics.
The possibility to use marker assisted selection for flooding tolerance is discussed.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
6.
Spelt wheat (Triticum spelta L.) is an old‐world crop that has attracted increased attention since the 1970s accompanied by a constant increase in spelt production. Our objectives were to (i) study the genetic variability and heritability of important agronomic traits and (ii) examine the correlation among these traits for an efficient design of spelt breeding schemes. To this end, we compared a diversity panel of 150 old and new spelt varieties with current elite‐breeding material. Phenotypic data were recorded at multiple locations for yield, plant height, lodging, heading time, number of spikelets, ear snapping and susceptibility to powdery mildew, brown rust and Septoria tritici blotch. We observed highly significant genetic variances for almost all traits in both germplasm sets. Heritability estimates ranged from 0.23 for Septoria tritici blotch to 0.97 for plant height. A significant correlation between yield and plant height of ?0.56 as well as yield and lodging of ?0.39 was determined but no other correlation of yield with any of the examined traits. Thus, pedigree selection with early generation screenings on plant height and lodging can be recommended for further spelt improvement. 相似文献
7.
Identification and molecular mapping of a leaf rust resistance gene in spelt wheat landrace Altgold 总被引：2，自引：0，他引：2
Yanjie Wang Huiru Peng Gang Liu Chaojie Xie Zhongfu Ni Tsomin Yang Zhiyong Liu Qixin Sun 《Euphytica》2010,174(3):371-375
Leaf rust, caused by Puccinia triticina, is an important disease for wheat production, both in China and worldwide. In laboratory studies spelt wheat (Triticum aestivum ssp. spelta) landrace Altgold was resistant to P. triticina races THT and PHT and genetic analysis indicated that it possessed a dominant leaf rust resistance gene, temporarily designated
LrAlt. F6 recombinant inbred lines (RILs) derived from a cross with the susceptible common wheat cultivar Nongda 3338 were used to
map LrAlt with SSR markers. The resistance gene was distal to SSR loci Xbarc212, Xwmc382, Xgwm636, and Xwmc407 on the short arm of chromosome 2A. The closest markers Xbarc212 and Xwmc382 which co-segregated were 1.8 cM away from LrAlt. The relationships of LrAlt and other wheat leaf rust resistance genes located on the short arm of chromosome 2A were discussed, suggesting that LrAlt might be a new leaf rust resistance gene. 相似文献
8.
Genetic analysis of bread-making quality in wheat and spelt 总被引：19，自引：0，他引：19
Bread‐making quality in wheat and spelt reflects the combination of several, mostly quantitatively inherited parameters. The aim was to find molecular markers linked to quantitative trait loci (QTL) for quality parameters. Zeleny sedimentation values (Zel), protein (Prot), kernel hardness (KH) and 1000‐kernel weight (TKW) of 226 F5 recombinant inbred lines (RILs) from a cross between wheat and spelt were assessed in different environments. The dough properties of 204 RILs were assessed with an alveograph. Based on a genetic map of 187 loci, nine QTL were found for Zel and Prot, explaining 47% and 51% of the phenotypic variance, respectively. Fifty‐four per cent of the variance was explained by 10 QTL for KH and eight for TKW. For the alveograph parameters 10 QTL were found for baking strength, nine for tenacity, seven for configuration ratio, and four for elasticity index and extensibility. The phenotypic variance explained ranged from 25% to 48%. The population mean of the dough parameters was shifted towards the spelt parent. It is concluded that non‐additive effects are crucial in the expression of high bread‐making quality of wheat. The consequences for wheat and spelt breeding programmes are discussed. 相似文献
9.
This study was conducted to assess regional patterns of diversity of Ethiopian tetraploid wheat accessions and to identify areas of diversity that can be used as source of new germplasm for developing high yielding and stable varieties. A collection of 133 Ethiopian tetraploid wheat accessions and eight introduced cultivars was analysed using 29 wheat microsatellite markers. A total of 383 alleles were detected with an average value of 13.14 alleles per locus. Relatively more alleles were observed in the B genome than in the A genome. Gene diversity indices ranged from 0.08 to 0.95, with a mean value of 0.72. Accessions collected from the same region were pooled and the number of alleles and gene diversity were calculated over the 29 simple sequence repeats for each region. Higher numbers of alleles were detected in the Shewa region (8.72), followed by Tigray (5.86) and Hararghe (5.76). The highest average gene diversity value was found in Shewa (0.65), followed by Gondar (0.64). No significant correlation was observed between geographic distance and genetic distance. Out of 383 different alleles detected, 93 (24.4%) were observed to be region‐specific. Region‐specific alleles were found across all chromosomes except for Xgwm752, Xgwm155 and Xgwm148. Genetic similarity coefficients were estimated for all the possible 55 pairs of regional comparisons and they ranged from 0.16 to 0.52, with a mean value of 0.50. All provinces were differentiated in the UPGMA cluster diagram. 相似文献
10.
The aim of this study was to estimate the extent of the genetic diversity present in sorghum germplasm grown in Kenya using simple sequence repeat markers. A total of 139 accessions were genotyped using 11 microsatellite markers or simple sequence repeats (SSRs) and the genetic diversity was estimated. The markers showed a wide range of differences in quality index from 0.005 to 0.39. The average Polymorphic Information Content value observed was 0.6241 indicating a high level of diversity. The gene diversity index ranged between 0.2419 and 0.9313 with a mean of 0.6627 per locus. A total of 105 alleles were observed with an average of 10.4 alleles per locus. The average heterozygosity level per locus was low at 0.1717. The variability within accessions among the populations was 74.85% and within individual accessions was 18.67%. The results showed that genetic diversity within Kenyan sorghum germplasm accessions is higher than that between the different populations. It is implied that such genetic diversity can be exploited as such or in hybridization programs to improve sorghum varieties currently grown by subsistence farmers in Kenya. 相似文献
11.
In order to reveal the molecular genetic diversity of wheat wild relatives, an AFLP analysis was conducted with 16 accessions
of five Triticum andAegilops species originating from the Near East. Variation within population was studied with at least seven individuals per accession.
Four primer combinations were used for selective amplification. Based on the scored bands, we estimated percentage of polymorphic
bands, 1 – proportion of shared bands (1-psb) and nucleotide diversity (π). Of the five species used in this study, Ae.
speltoides had the highest level of `within population' variation. This species had also the highest value of the variation among populations.
As for Triticum species, the level of variation within population was low in diploid species (T. urartu and T. boeoticum),whereas two tetraploid species (T. dicoccoides and T. araraticum) had relatively high levels of variation within population. While the two diploid Triticum indicated a clear interspecific divergence, the two tetraploid wild wheats were not clearly divergent in this study. The
variance portioning analysis indicated that the variation detected for diploid Triticum species was mainly composed of `between species' variation, on the other hand that for tetraploid Triticum was mostly composed of `within population' variation. In conclusion, AFLP analysis reveals molecular variation in all accessions
used in this study, suggesting a potential genetic diversity of the wheat wild relatives in natural populations. These results
have implications for the design of strategies to maintain genetic diversity within genebank collections.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
12.
Genetic variability in melon based on microsatellite variation 总被引：8，自引：0，他引：8
A set of 18 simple‐sequence repeat (SSR or microsatellite) markers was used to study genetic diversity in a collection of 27 melon (Cucumis melo L.) accessions, representing a broad range of wild and cultivated melons. The materials studied were highly polymorphic for SSRs and a total of 114 alleles were detected (average of 6.3 alleles per locus). Cluster analysis suggests the division of these accessions into two major groups, largely corresponding to the division of C. melo in the two subspecies agrestis and melo. The assignment of the accession to the subspecies was generally in agreement with published reports, except for those corresponding to the ‘dudaim’ and ‘chito’ cultivar groups, which, according to the observed SSR variability, should be included in subspecies agrestis. Based on cluster analysis, five groups of accessions were defined. The two most divergent groups include mainly accessions from the Mediterranean which form one group, and accessions from China, Japan, Korea and India forming the other. Both groups shared a low level of intra‐accession variation compared with the other groups, which suggests an erosion of their genetic variability because of drift and/or inbreeding. The remaining accessions, mainly from Central Africa and India, were more variable and may be an important source of genetic variation for melon breeding. 相似文献
13.
《European Journal of Agronomy》2005,22(3):293-300
Einkorn (Triticum monococcum L.), emmer (Triticum dicoccum Schübler) and spelt (T. spelta L.) are still cultivated in Italy. These three hulled wheat species are more commonly known as “Farro”. Little is known about agronomic practices that optimise the grain yield of these species.This study has been carried out to establish the appropriate seeding rate for einkorn, emmer and spelt which is grown in southern Italy (Apulia region), a typical Mediterranean environment, where durum wheat is principally cultivated. Two years of experimental field trials were conducted with three seeding rates (100, 150 and 200 viable seeds per square meter).Emmer had the highest hulled grain yield (3.54 t ha−1) followed by spelt (2.80 t ha−1) and einkorn (1.42 t ha−1). Emmer also had a higher kernel weight and was heading earlier than the other species. The bad performance of einkorn can be accountable to the excessive time to reach heading and the natural inclination of plants to lodge, factors that reduce the ability of plant to complete grain ripening, resulting in light and shrivelled kernels. The lower grain yield of spelt in comparison to emmer may be due to later heading.Emmer and spelt performed the best when they were sown at 200 seeds m−2 (3.85 and 3.09 t ha−1, respectively). In contrast, einkorn showed the highest grain yield (1.69 t ha−1) at the lowest seeding rate (100 seeds m−2). Further, additional experimentation is required to confirm this.These results indicate that emmer is the most appropriate hulled wheat species for cropping under southern Italy’s growing conditions, and provide further information about the use of these species in the marginal area preservation or when the cultivation of economically profitable crops is precludes by water deficiency and soil poorness. 相似文献
14.
Gliadin alleles were identified in 100 common wheat cultivars registered and/or grown in Spain during the last 40 years. A very high level of genetic polymorphism was found: in total, 103 allelic variants including one null‐allele were found at the six major Gli loci in the Spanish wheats studied. An average genetic diversity for these six loci was found to be higher (H=0.844) than in any group of wheat cultivars studied previously. Spanish wheats bred in Spain demonstrated even higher genetic diversity (H=0.868), probably because of the occurrence in this group of some landraces (local varieties) assumed to be strongly differentiated to fit local environments. The high level of genetic diversity of wheats grown in Spain was maintained by the introduction of distantly related wheat germplasm from different sources, especially from Italy and CIMMYT. A slight decrease of genetic diversity in recently registered cultivars might be caused by the excessive introduction of French wheats. Thirteen new alleles found in Spanish wheats were catalogued, including Gli‐D2w which encodes the first Gli‐D2‐controlledγ‐gliadin to be found. 相似文献
15.
Granule‐bound starch synthase of ancient wheat and related species was examined by sodium dodecyl sulphate polyacrylamide gel. A total of 13 different alleles were revealed in a collection of three accessions of diploid wheat, six accessions of tetraploid wheat, 49 accessions of spelt wheat, nine accessions of Sitopsis and two accessions of Aegilops tauschii. A new allele named Wx‐A1a′ appeared in four spelt wheat accessions. The tetraploid wheat accessions evaluated did not show any polymorphism; nevertheless the tetraploid accessions of Sitopsis section revealed three novel alleles. The novel allele Wx‐Ddn1g was found in two accessions of A. ventricosa and the Wx‐Ddcm1h and Wx‐Ddcm1i in two accessions of A. crassa. A novel allele named Wx‐Au1g was found in Triticum urartu, which is different from the also new Wx‐Am1h allele of T. monococcum. The diploid‐related species accessions revealed two novel alleles named Wx‐Bsl1h and Wx‐Bs1g found, respectively, in A. longissima and A. speltoides. The amylose content was measured for the different alleles found in all evaluated species and no significant effects of the allele composition on the amylose content were detected. 相似文献
16.
Fuminori Kobayashi Tsuyoshi Tanaka Hiroyuki Kanamori Jianzhong Wu Yuichi Katayose Hirokazu Handa 《Breeding Science》2016,66(2):213-225
A core collection of Japanese wheat varieties (JWC) consisting of 96 accessions was established based on their passport data and breeding pedigrees. To clarify the molecular basis of the JWC collection, genome-wide single-nucleotide polymorphism (SNP) genotyping was performed using the genotyping-by-sequencing (GBS) approach. Phylogenetic tree and population structure analyses using these SNP data revealed the genetic diversity and relationships among the JWC accessions, classifying them into four groups; “varieties in the Hokkaido area”, “modern varieties in the northeast part of Japan”, “modern varieties in the southwest part of Japan” and “classical varieties including landraces”. This clustering closely reflected the history of wheat breeding in Japan. Furthermore, to demonstrate the utility of the JWC collection, we performed a genome-wide association study (GWAS) for three traits, namely, “days to heading in autumn sowing”, “days to heading in spring sowing” and “culm length”. We found significantly associated SNP markers with each trait, and some of these were closely linked to known major genes for heading date or culm length on the genetic map. Our study indicates that this JWC collection is a useful set of germplasm for basic and applied research aimed at understanding and utilizing the genetic diversity among Japanese wheat varieties. 相似文献
17.
Common bunt caused by Tilletia tritici and T. laevis has occurred worldwide and reduces yield and quality in common and durum wheats. The development of DNA markers linked to bunt resistance to race T1 in the cross, ‘Laura’(S) בRL5407’ (R), was carried out in this study based on the single head derived F4:5 and single seed derived F4:6 populations. Bulked segregant analysis was used to identify two random amplified polymorphic DNA (RAPD) markers linked to the gene for resistance to race T1 in the spelt wheat ‘RL5407′. The two markers identified, UBC548590 and UBC274988, flanked the resistance gene with a map distance of 9.1 and 18.2 cM, respectively. The former was linked in repulsion phase to bunt resistance while the later was in coupling phase. The two RAPD markers and the common bunt‐resistance gene all segregated in Mendelian fashion. Use of these two RAPD markers together could assist in incorporating the bunt‐resistance gene from spelt wheat into common wheat cultivars by means of marker‐assisted selection. 相似文献
18.
Muigai Paul Kimani Erick Kimutai Cheruiyot James Otieno Owuoche Francis Nyamu Wachira Esther Kimani 《Journal of Crop Science and Biotechnology》2014,17(2):97-102
The genetic diversity of 65 accessions of sorghum [Sorghum bicolor (L.) Moench] collected from various farmers and germplasm lines from ICRISAT-Kenya were analyzed. Simple sequence repeats (SSR) markers were used in order to determine the extent and distribution of its genetic diversity. Twenty-nine (29) SSRs markers were polymorphic and a total of 192 alleles were detected which showed diversity. The number of alleles per primer ranged from 2 to 17, with an average of 6.62. The range of polymorphism information content (PIC) ranged from 0.03 to 0.86, with total average of 0.82. According to the results analyzed, estimates of the mean allelic pattern across the two populations was generated; expected heterozygosity (He; 0.45, 0.54), average observed alleles (Na; 3.40, 6.20), number of private allele (0.23, 3.03), and Shannon information index (I; 0.85, 1.13) for farmer and ICRISAT-Kenya germplasm, respectively. The expected heterozygosity (He) varied from 0 to 0.26 with an average of 0.05. The Neighbor-joining phenogram based on Nei’s genetic distance grouped the 65 accessions into three main groups. The analysis of molecular variance (AMOVA) revealed that 99% of the total genetic variation was within accessions in a population whereas the genetic variation among populations in accessions accounted for 1% of the total genetic variation. Genetic diversity in ICRISAT sorghum material compared to the farmer’s collection suggested little infiltration of improved germplasm to the farmers. 相似文献
19.
An analysis of Spanish land races of Avena sativa from the Germplasm Bank of the I.N.I.A. was carried out using starch gel electrophoresis. Samples of 15 seedlings per accession were used for estimating the intra- and interpopulational genetic variability. Electrophoretic analyses were performed with crude extracts from 15 day-old seedling leaflets by means of eight isozymatic systems representing a total of ten genetic loci. Two systems were mono-morphic in all the accessions, while the other six showed polymorphism both within and between populations. Intrapopulational variability was particularly high in this collection, only 16 out of the 137 accessions being uniform for all the isozyme systems. Thus the material was shown to represent a high potential of genetic variability for breeding programmes. 相似文献
20.
Fifteen amplified fragment length polymorphism (AFLP) EcoRI/MseI‐based primer combinations with five selective bases (Eco RI‐ANN, MseI‐CN) were used to estimate genetic diversity among 45 line varieties of cultivated cornsalad and 19 genebank accessions classified into nine different species related to cornsalad. Polymorphic fragments were scored for calculation of Jaccard's coefficient of genetic similarity (GS). The average GS estimate in elite germplasm (GS = 0.90) was substantially higher than in exotic germplasm (GS = 0.47). UPGMA‐cluster analysis revealed genetic relationships among recently bred varieties, old varieties and genebank accessions. Analysis of molecular variance indicated almost threefold variability within sets compared with between sets due to a high level of polymorphism among wild species. Sources for increasing genetic diversity in elite germplasm of cornsalad were suggested and a duplicate among the genebank accessions was detected. AFLPs could be considered a powerful tool for genetic diversity estimation in cornsalad germplasm and are recommended for systematic fingerprinting of remaining cornsalad species. 相似文献