共查询到17条相似文献，搜索用时 234 毫秒
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9种检疫性实蝇PCR-RFLP快速鉴定研究 总被引：12，自引：1，他引：12
应用PCR-RFLP技术,对我国口岸截获频率较高的9种检疫性实蝇开展了快速鉴定方法的研究,结果表明,设计的2对引物对9种供试实蝇线粒体DNA(mtDNA)的PCR扩增片断大小分别约为350bp和450bp.用限制性内切酶MSE1和DRAI对PCR扩增产物进行酶切,得到的酶切位点可将供试的9种实蝇区分开来.该方法不受供试实蝇地理来源及食物源的影响,对各种虫态(卵、幼虫、蛹)和不同性别的成虫均适用,可用于口岸截获实蝇的快速检疫鉴定. 相似文献
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《植物检疫》2019,(4)
蜜柑大实蝇(Bactrocera tsuneonis)和橘大实蝇(Bactrocera minax)属果实蝇属(Bactrocera)大实蝇亚属(Tetradacus),是为害柑橘类作物的两种检疫性实蝇。两种实蝇为近似种,仅成虫可通过形态特征进行鉴定,幼虫等非成虫虫态需采用分子生物学技术才能实现快速、准确的鉴定。本研究对采自云南省昭通市大关县、彝良县和永善县的大实蝇成虫及幼虫样品分别进行了形态鉴定和DNA条形码鉴定。结果表明,通过形态特征的对比以及DNA条形码序列的测定、比对和分析,采自大关县和彝良县的20头成虫样品分别为蜜柑大实蝇和橘大实蝇,采自彝良县和永善县的20头幼虫样品均为橘大实蝇。本研究实现了云南昭通地区两种大实蝇的DNA条形码鉴定,为我国植物检疫相关部门提供实用技术及决策支持。 相似文献
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实蝇是世界重要的检疫性害虫之一,对果蔬生产及其国际贸易具有很大的影响。而以线粒体DNA细胞色素C氧化酶亚单位I(mtDNACOI)基因的部分序列作为实蝇的DNA条形码能减少对实蝇成虫形态特征的依赖,可检测其任何虫态的样品,有助于实蝇样品的快速鉴定。本研究采用DNA条形码技术,针对采自泰国四色菊市番石榴烂果中的5头实蝇幼虫进行COI扩增测序,与生命条形码数据库(BOLD)中的序列进行比对并利用PAUP4.0软件构建了其系统进化树。根据序列分析和系统进化关系分析的结果,将5头实蝇幼虫样品鉴定为番石榴实蝇(Bactrocera correctaBezzi),并将本研究获得的2条COI序列在GenBank中注册,GenBank的登录号为HM590450和HM590451。 相似文献
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对进境旅客携带芒果进行检疫时,在一枚芒果上发现了25头实蝇科幼虫,化蛹后显示存在两种形态特征差异较大的蛹,其中类型I的蛹10头,类型II的蛹15头。类型I中的5个蛹成功羽化为成虫,经形态鉴定为非洲芒果实蝇。类型II的蛹未成功羽化。由于对蛹的鉴定尚有困难,分别提取了两种类型蛹的线粒体DNA,分别扩增了COI、COII、COIII基因,测序结果与Gen Bank数据库进行序列比对分析。结果显示,类型I蛹COI、COII、COIII基因序列分别与非洲芒果实蝇相应基因的序列相似性达到99%以上,与形态学鉴定结果相同。类型II蛹COI基因序列与入侵果实蝇的基因序列相似性达到100%。综上结果,类型I幼虫为非洲芒果实蝇,而类型II为入侵果实蝇。在同一枚芒果上发现两种不同的检疫性实蝇,比较少见,增加了水果检疫的难度,在实际工作中应给予高度重视。 相似文献
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为了解山东省樱桃主产区果蝇的发生情况,本研究选择了山东省主要樱桃产区的7个地点进行调查,并通过形态学特征和分子鉴定法对果蝇的种类进行鉴定。结果发现,山东省樱桃主产区有4种果蝇,通过形态特征与分子鉴定法最终确定4种果蝇,分别为斑翅果蝇Drosophila suzukii、黑腹果蝇D.melanogaster、海德果蝇D.hydei和伊米果蝇D.immigrans。这4种果蝇对樱桃的为害程度与种植地区、果实腐烂程度和樱桃的品种均有关系;莱芜和泰安地区果蝇分别有4种和3种;烟台和威海地区则只有斑翅果蝇和黑腹果蝇2种。烟台地区果蝇为害率最高,且数量最多,其中烟台丁家夼村樱桃为害率高达45.16%,在第2次采集中烟台薛家村每100粒樱桃中斑翅果蝇和黑腹果蝇的数量最多。美早和先锋2个品种樱桃对果蝇的吸引力较强;采摘后期腐烂的果实可吸引大量的果蝇产卵。因此,应对烟台地区进行重点预防、早期防治,并减少美早和先锋品种樱桃在山东省的种植。 相似文献
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Sorada Tapsuwan Tim Capon Mia Tam John Kandulu Penny Measham Stuart Whitten 《国际虫害防治杂志》2020,66(4):351-367
AbstractFruit flies are a biosecurity threat to international trade of horticultural produce around the world. Area-wide management (AWM) of fruit flies offers a promising solution to coordinate farmers’ activities to fight against the spread of this pest. The incorporation of the sterile insect technique (SIT) may increase the effectiveness of AWM and lead to greater suppression and possible eradication of the fruit fly pest. This article presents findings from a choice experiment survey to ascertain the willingness to pay (WTP) for the benefits of a suite of AWM features and SIT. These features include the benefits from (1) coordinated fruit fly monitoring, (2) coordinated on-farm male annihilation of fruit flies, (3) management of fruit fly in residential areas, and (4) coordinated release of sterile flies. This is the first study of its kind to assess the WTP for AWM and SIT in a developed country. Results suggest that growers are willing to pay for SIT and coordinated fruit fly management in residential areas but are not willing to pay for other coordinated services that they are already practicing on their farms. 相似文献
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实蝇科昆虫种类繁多、危害巨大、入侵能力强,倍受植物检疫与入侵生物学领域的关注。转录组代表细胞或组织内全部的RNA转录本,反映特定状态下的基因表达模式。而依赖于高通量测序技术所衍生出的转录组测序给实蝇等非模式生物的研究带来了机遇。本文在检索实蝇科昆虫转录组序列数据和文献报道的基础上,针对实蝇科昆虫转录组的测序现状和初步应用进行了分析和总结,涉及重要经济实蝇的种类鉴定、基础生理、滞育、配子形成和交配生殖、解毒机制及防治和非编码RNA共6个方面。同时,提出了加强重要经济实蝇转录组研究的3个展望:一是加强结构基因组学和功能基因组学方面的多组学结合研究;二是开展microRNA(miRNA)等非编码RNA功能方面的探索研究;三是探索第三代测序技术的应用研究。 相似文献
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A technique for rearing the progeny of wildCeratitis capitata flies was developed. The method is based on wild captured flies which are allowed to oviposit in artificial fruit containing
larval rearing medium. Flies develop in the fruit from eggs to last larval instar, then exit the fruit and pupate within vermiculite.
This method was shown to be feasible and efficient, and to increase considerably the number of wild flies for testing purposes.
Possible contribution to the Sterile Insect Technique (SIT) and potential applications of the method are discussed. 相似文献
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K. Armstrong 《EPPO Bulletin》2010,40(1):91-100
Molecular methods for identification of high risk pests and pathogens have been employed for more than a decade to supplement standard diagnostic protocols. However, as the volume of traded goods continues to increase so does the breadth of taxa that diagnosticians need to deal with. Keeping pace by introducing more molecular tests that are typically species-group specific is not an efficient way to progress. Since 2005 classical DNA barcoding using cytochrome oxidase I sequence has been employed routinely in New Zealand for the highest risk insect species (fruit flies and lymantriid moths). Subsequently a broader range of pests have been considered. Case studies are presented here for three important lepidopteran pests, Lymantria mathura (pink gypsy moth), Conogethes punctiferalis (yellow peach moth) and Hyphantria cunea (fall web worm), as well as a trial to identify miscellaneous border interceptions. While the data support the effectiveness of DNA barcoding for border diagnostics, they also raise issues around cryptic species identification and potential species discovery that could impact on operational biosecurity systems. 相似文献
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简述了在缅甸东北部监测发现的5种检疫性实蝇——泰实蝇(Bactrocera(Bactrocera) thailandica Drew & Hancock)、越南实蝇(Bactrocera(B.)yoshimotoi(Hardy)、黑颜实蝇(Bactrocera(Zeugodacus) diaphora(Hendel))、黑颜面实蝇(Bactrocera(Zeugodacus) vultus(Hardy))和瓜棍腹实蝇(Dacus(Callantra) longicornis(Wiedemann))的形态特征,并附鉴别特征图,为口岸检疫提供帮助。 相似文献