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1.
Two methods of induction of ovulation were examined in Manchega ewes that were in postpartum anestrus during the nonbreeding season. The experiment was of 2 X 2 X 3 factorial design. The methods compared were introduction of rams and this treatment plus an im injection of 50 micrograms luteinizing hormone releasing hormone (LHRH) at the time rams were introduced. Variations in response due to month of treatment (April, May or June) and interval from lambing to treatment (1, 2 or 3 mo) and their interactions with type of treatment were examined. Responses studied were proportions of ewes showing increases in plasma progesterone at (a) 10 d or (b) 17 or 24 d post-treatment, or lambing by 200 d post-treatment, and interval from treatment to lambing for ewes that did lamb by 200 d. The formation of a corpus luteum was determined by concentrations of progesterone in plasma; a positive response was considered to be a concentration greater than .5 ng/ml (baseline values averaged .1 ng/ml). Overall, there was no benefit of LHRH above the response to introduction of males only, in any trait examined. There was a significant interaction of treatment with month of treatment on the proportion of ewes forming corpora lutea by 17 or 24 d after initiation of treatment. This proportion was lower in June (38 vs 66% in April and 82% in May) for ewes receiving LHRH, but did not differ among months (61 to 68%) for ewes exposed to males only.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

2.
程薪宇  刘玫  郭梦桥 《草业学报》2015,24(3):168-176
本文采用形态观察及GMA半薄切片法深入研究了毛茛科18属,37种,及5变种的雄蕊形态结构。结果显示雄蕊表面无毛或被非腺毛及腺毛。花丝呈丝状、条状、棒状、长三角形或短柱状,其薄壁组织胞间隙不明显或发达。花药侧面观为∞形、椭圆形、近圆形或条形;幼嫩花药横切面形状变化较大,为V形、Λ形、椭圆形、方形、∞形或蝶形;花粉囊间薄壁细胞排列呈V形、Λ形或直线形。成熟花药的花粉囊间薄壁细胞消失。部分幼嫩时横切面∞形的花药,成熟时变为椭圆形,而蝶形和方形的花药则变为∞形。花药及花丝的特征在属及种内是稳定的,可作为毛茛科属及组的分类学证据。本文完善了毛茛科花形态学研究,为进一步研究毛茛科系统学提供了新的形态学基础。  相似文献   

3.
Between 1994 and 2001, guttural pouch tympany was diagnosed in 51 foals; there were approximately three times as many fillies as colts, of Arabian, different German warmblood breeds and Western horse breeds. There were significantly more Arabian and paint horse foals than expected in comparison with the breed distribution of the foals hospitalised at the Clinic for Horses. The foals' breed and sex did not influence the age of onset, the type and severity of the clinical signs or the recurrence rate. A surgical laser technique was used on 50 of the foals; in 35 cases only one surgical treatment was necessary, in seven cases a second operation was required during the foal's initial period of hospitalisation, and in eight cases a second operation was performed during a second period of hospitalisation. Long-term follow-up information was obtained for 44 of the 50 treated horses; 24 of them were under two years of age and 20 were over two years of age. In six horses, no follow-up information was available. Four horses were euthanased for reasons unrelated to the condition or its treatment. The horses over two years of age were in training or were being used for competitions in dressage or jumping or for breeding purposes, and in only one of them was an adventitious respiratory noise reported. All the horses up to two years of age were reported to be healthy.  相似文献   

4.
Twenty-five cases of feline bronchial disease were identified retrospectively. The criteria for inclusion were consistent clinical signs or histopathology and no other identifiable aetiology. Patient records were analysed to determine historical, clinical, clinicopathologic and radiographic features. The main presenting complaints were coughing and dyspnoea. The most common physical finding was dyspnoea. The majority of radiographs had a bronchial pattern either as the sole change or as a component of a mixed pattern. Bronchoalveolar lavage cytology was neutrophilic or eosinophilic in the majority of cats. There was no association between age, breed, sex, clinical signs, bronchoalveolar lavage cytology or radiographic severity and disease severity.  相似文献   

5.
Both conventional and specific pathogen free pigs were inoculated intranasally with a strain of Aujeszky's disease virus (ADV). Nasal cells were collected daily by swab, aspiration or wash. The nasal cells were examined for ADV by isolation on cell culture, direct or indirect immunofluorescence and immunoperoxidase staining by monoclonal antibodies. The infected pigs were studied for nasal shedding of infected cells until 30 days after infection. The study was also extended to naturally infected farm pigs. Swabbing, washing and aspiration proved effective methods of collecting between 10(5) and 10(8) pavement or columnar epithelial cells and non-epithelial cells. Macrophages and polymorphonuclear leucocytes were also identified. Infected nasal cells were detected by immunofluorescence and immunoperoxidase from one to 21 days after infection. The viral antigen was detected in both epithelial and non-epithelial cells, the fluorescence was nuclear and, or, 'cytoplasmic', in the latter case only the cell membrane was stained. ADV antigens were detected in nasal cavity cells in pigs infected with a virulent and a hypovirulent strain. Nasal swabs proved effective in confirming infection both by virus isolation and immunological assay, and the latter was shown to be a useful experimental tool for the rapid diagnosis of Aujeszky's disease virus infection in fattening pigs suffering from acute respiratory distress.  相似文献   

6.
Pyotraumatic dermatitis (hot spot) is a common clinical syndrome in dogs but there are few prospective scientific studies related to it. The aim of this study was to investigate correlations among clinical pyotraumatic dermatitis, histopathology of the lesions and possible predisposing causes. The relationship of these with breed, age, sex and location of lesion was assessed statistically. A clinical diagnosis of acute pyotraumatic dermatitis was made in 44 privately owned dogs. Males exceeded females (P = 0.0348) and lesions were more common in dogs aged 4 years or less (P < 0.0001). Lesions were most often seen on the cheek, neck and lateral thigh with a significant correlation between breed and site of lesion (P < 0.0001). In 31 cases a possible underlying cause was found or suspected. In contrast to previous studies, no otitis externa was recorded and the study was conducted in an area without endemic fleas. Fourteen breeds were represented of which Rottweiler, German shepherd dog and golden retriever were most common. There was no significant seasonal incidence and no correlation among site of lesion and cause, time of year, age or sex. Histopathologically, the dogs could be separated into four patterns by the presence or absence of eosinophils and/or folliculitis. Eosinophils have not previously been recorded in pyotraumatic dermatitis but were seen in 29 cases. Acute folliculitis was seen in 20 cases. However, no correlation was seen among age, sex, breed, underlying cause or site of lesion and histopathology. Twenty-seven cases were cultured for bacteria of which 25 grew Staphylococcus intermedius and two were negative.  相似文献   

7.
SUMMARY A survey of the attitudes of dog owners to desexing male and female dogs was conducted in the Brisbane area. Over 15800 replies (35% response) were returned for analyses and included both demographic and attitude data. The most common reasons given by owners for not having the dog desexed were that it was unnecessary or they wanted to breed from it or could not afford it or did not agree with it or that the dog was too old or it might get fat. Nearly twice as many male owners agreed that desexing male dogs removed maleness and also more males agreed with the question “Do you equate dog sexuality with human sexuality?” Female owners were more aware than males that their female dogs did not need to have a season (oestrus) or a litter before being desexed. More male owners thought that desexing changed a male or female dog's personality. A disturbing fact was that 61.1% of male owners and 47.3% of female owners would now not have their dog desexed if it had not already been done. More male than female dog owners were ignorant about whether desexing changed personality, made dogs frustrated, or if female dogs should have a season or a litter before desexing. The study showed a gender bias in owner attitudes to all the attitude questions.  相似文献   

8.
Normal canine hip cartilage was compared with cartilage from the degenerative lesions found in young dogs with canine hip dysplasia. The upper 0.5 mm of normal cartilage was characterized. Four distinct layers or zones were found: a layer of fine fibrous material covering the surface, a layer (surface layer) of small (32 nm diameter or less) collagen fibrils tightly packed in bundles and oriented parallel to the surface, a layer (upper layer) or less tightly packed collagen fibrils oriented mostly parallel to the surface with about 33% of the fibrils 64 nm or more, and a layer (intermediate layer) of randomly oriented fibrils with more than 50% of the fibrils 64 nm or larger. Fibril density was high in the surface layer and decreased with depth into the cartilage. In a moderately advanced lesion of degenerative cartilage, there was a layer of amorphous material over the surface. The tightly packed surface layer of small fibrils was absent. The surface itself was uneven and fissued. At depths from the surface comparable to the upper and the intermediate layers in normal cartilage, the proportion of large fibrils was less than in normal cartilage. The overall density of fibrils in degenerative cartilage increased with depth into the tissue. Cells flattened parallel to the surface, with relatively large nuclei, were found in the upper layer of normal cartilage. Cells in the intermediate layers were larger and round. The oblong cells of the upper layer of normal cartilage were not found in any layer of degenerative cartilage. Differences between cells in other layers of normal and degenerative cartilages were minimal. A model for the arrangement of chondrocytes and collagen fibrils for normal and degenerative cartilage was proposed. Ultrastructural changes in degenerative cartilage were prominent in the upper 0.5 mm of cartilage. These changes were changes in the number of collagen fibrils/mum-2 and a change from a characteristic pattern of collagen fibril diameters and orientation found in normal tissue.  相似文献   

9.
The purpose of this study was to test the effectiveness of a commercially available early pregnancy test in cows. The test early conception factor (ECF™) was designed to distinguish between open and pregnant cows by detection of a protein present in blood serum or milk of pregnant cows. All tests were conducted on blood serum according to instructions of the manufacturer. Blood samples were taken via tail vein puncture between 6 and 15 d post-insemination from 67 Holstein or Jersey cows. Serum was placed in a test cassette and the results recorded after 2 h by three independent observers. Ultrasonography was performed on d 25 to 30 post-insemination, or rectal palpation was performed 35 to 42 d post-insemination by experienced personnel. These results were compared with the test results. The test results corresponded with the results of the ultrasound or rectal palpation 51% of the time. Of the 49% that disagreed, 25% were diagnosed pregnant by the ECF test but were found to be open by ultrasound or palpation, and 24% were diagnosed open by the test and found to be pregnant by ultrasound or palpation. These results suggest that the test does not perform at the necessary level of accuracy.  相似文献   

10.
A field investigation of kennel cough: Efficacy of different treatments   总被引:2,自引:0,他引:2  
The efficacy of antibiotics, corticosteroids and antitussives in the treatment of kennel cough was investigated in clinical cases in the field, using information recorded on questionnaires distributed to a random sample of veterinary practitioners in the United Kingdom. Analysis of the results demonstrated a statistically significant difference in the duration of coughing between treated and untreated dogs when a trimethoprim-sulphonamide combination, oxytetracycline, ampicillin/amoxycillin, or corticosteroid were administered alone, and when ampicillin/ amoxycillin-corticosteroid or trimethoprim-sulphonamide-corticosteroid combinations were given. The estimated median duration of coughing between treated and untreated animals when a trimethoprim-sulphonamide combination or ampicillin/amoxycillin were administered alone also differed significantly. The most efficacious drug was trimethoprim-sulphonamide, administered alone.  相似文献   

11.
Certain cytologic features of the porcine adrenal medulla   总被引:1,自引:0,他引:1  
Adrenal glands were collected from pigs of various ages under general anesthesia. Glutaraldehyde-fixed medullary tissue was postfixed with OsO4 for electron microscopy and with potassium dichromate or potassium iodate for light microscopy. Columnar epinephrine (E) cells formed cords between wide sinusoidal capillaries at the corticomedullary junction and were arranged in palisade fashion along the central vein and its major tributaries. The E cells usually were polarized, with the nuclei located away from the sinusoidal capillaries. Clusters of polygonal norepinephrine (NE) cells formed large central aggregates surrounded by E cells. Granulated vesicles were the predominant cytoplasmic feature of both E and NE cells. Round or oval E granules were bounded by a crenated membrane separated from the granule by a clear halo. The more electron-dense, elongate NE granules were bounded by a closely apposed, smooth membrane. The average longest granule axis was 270 nm for E granules and 305 nm for NE granules. Many cytoplasmic organelles were congregated in a granule-free paranuclear zone, which contained a prominent Golgi complex. Thin nonmyelinated nerve fibers (singly or in small groups) were interposed between the E and NE cells. Nerve fibers often were located close to the nucleus in a depression of the cell surface and often were wrapped by thin E or NE cell processes. The medulla of newborn pigs was composed predominantly or exclusively of NE cells. In both adults and pigs, E or NE cell cords radiated through the cortex toward the capsule, and isolated clusters of E or NE cells frequently were found in the capsule or zona glomerulosa.  相似文献   

12.
The structure of double and multiple flagella of the spermatozoa of a drake was investigated. In spermatozoa with this defect one or two implantation pits were observed in the head of the spermatozoon with one or two proximal centrioles situated in the extended base of the nucleus. The structure of the proximal centrioles was frequently disturbed. Distal centrioles were placed separately without any marked figural or structural disturbance. The mitochondrial sheath was formed around both or more flagella, and between the flagella only one line of mitochondria was usually found. The ring showed defects in shape and changes in the deposition. An amorphous sheath was formed around each flagellum separately without any marked defects. In single cases insignificant changes were recorded in the arrangement of fibrils of the axial bundle.  相似文献   

13.
Seventy-nine cattle in all stages of gestation were inoculated with a low dose (2.5 x 10(8) colony-forming units) of Brucella abortus strain 19, then challenge exposed with pathogenic B abortus strain 2308 during the subsequent gestation. A brucellosis case was defined by isolation of strain 2308 from dam or calf samples. Cumulative incidence of brucellosis cases was 48, 33, 25, or 47% for cattle that were, respectively, not pregnant, or 19 to 87, 100 to 167, or 190 to 253 days in gestation at vaccination. The cumulative incidence was 56% in 27 nonvaccinated controls. The 95% confidence intervals for risk ratios included 1 in all cattle, except those that were 100 to 167 days in gestation at vaccination (ie, second trimester); the confidence interval for this group was 0.21 to 0.97. The prevented fraction (1-risk ratio) attributed to strain 19, in ascending order, was 0.14, 0.16, 0.4, or 0.55, respectively, for cattle that were not pregnant, or were 190 to 253, 19 to 87, or 100 to 167 days in gestation at vaccination. Potential confounders of breed, pen effect, and gestation days at challenge exposure did not significantly affect results. Results supported the hypothesis that stage of gestation at vaccination will affect the prevented fraction of brucellosis, or efficacy of strain 19, in cattle vaccinated with a low dose and, therefore, is one factor that may explain variation in strain 19-induced protection.  相似文献   

14.
The purpose of this study was to test the effectiveness of a commercially available early pregnancy test in cows. The test early conception factor (ECF™) was designed to distinguish between open and pregnant cows by detection of a protein present in blood serum or milk of pregnant cows. All tests were conducted on blood serum according to instructions of the manufacturer. Blood samples were taken via tail vein puncture between 6 and 15 d post-insemination from 67 Holstein or Jersey cows. Serum was placed in a test cassette and the results recorded after 2 h by three independent observers. Ultrasonography was performed on d 25 to 30 post-insemination, or rectal palpation was performed 35 to 42 d post-insemination by experienced personnel. These results were compared with the test results. The test results corresponded with the results of the ultrasound or rectal palpation 51% of the time. Of the 49% that disagreed, 25% were diagnosed pregnant by the ECF test but were found to be open by ultrasound or palpation, and 24% were diagnosed open by the test and found to be pregnant by ultrasound or palpation. These results suggest that the test does not perform at the necessary level of accuracy.  相似文献   

15.
Clinical signs, causative factors, radiographic findings, type and duration of treatment or reason for killing were evaluated in 53 cattle (mean age: 5.3 years) suffering from toe ulcer and/or apical pedal bone necrosis. A total of 78 claws were affected. Four cattle suffered from a toe ulcer in one claw, 35 cattle showed osteolysis of the apex of the distal phalanx in a single claw and 14 cattle in two or three claws per cow. Overtrimming by means of a grinding disc and/or perforation of the sole was diagnosed as the major cause in 27 cattle (49%), laminitis in 30.2% and traumatic injuries in 11.3%. Radiography revealed a varying degree of osteolysis involving up to two-thirds of the pedal bone. Twenty-one cattle (39.6%) showing multiple toe disorders or involvement of one single claw with concurrent internal diseases were destroyed. In 23 cattle, the osteolytic bone was resected using a bone curette or hammer and chisel. Of these, the treatment was successful in 20 animals. The healing period ranged from 16-60 days when one claw was affected and from 43-53 days when two claws were affected. In five cattle, the digit was amputated.  相似文献   

16.
A total of 307 brains of purebred sows obtained from an abattoir were retrospectively examined. These sows were culled with reasons of reproductive failure, urogenital infections, or locomotor problems. The most common macroscopic lesions were cavitations or lacunae in the basal nuclei (9.1%, 28/307) and coarse and thickened leptomeninges with marked vessels (3.9%, 12/307). The most frequent microscopic lesion was polyarteritis nodosa (21.2%, 65/307), which was found in all 40 brains with the above-mentioned gross lesions and in all 25 brains with microscopic cerebral infarcts or cavitations. The affected arteries of polyarteritis nodosa were distributed primarily in the cerebral leptomeninges, basal nuclei, and internal and external capsules. Histopathologically, a characteristic change of the affected arteries was transmural fibrinoid necrosis with severe infiltration of mixed inflammatory cells; narrowing or occlusion of the lumen. The inflammatory cells were chiefly composed of lymphocytes, macrophages, and plasma cells, with a few eosinophils and occasional multinucleated giant cells. Polyarteritis nodosa was found at a high percentage in the brains from culled sows. It may result in cerebral ischemia, infarcts, and hemorrhage, and possibly play a role in the necessity for culling due to locomotor problems.  相似文献   

17.
Financial analysis of pseudorabies control and eradication in swine   总被引:1,自引:0,他引:1  
Computerized decision-tree analysis and simulation modeling were used to evaluate control and eradication strategies for pseudorabies virus (PRV) in swine. Three alternative actions were considered for a hypothetical 100-sow, farrow-to-finish operation: (1) depopulation-repopulation, (2) test-and-removal of seropositives (T&R), and (3) vaccination (of the entire herd or of sows only). The expected monetary values for the vaccination and T&R alternatives were similar, which was consistent with the long-standing controversy over the best strategy for dealing with PRV. When the prevalence rate of PRV was less than or equal to 57%, T&R was found to be optimal; otherwise, vaccination of sows only was recommended. Sensitivity analysis was performed to determine how modifications in some of the original assumptions affected the expected monetary values of each strategy. When higher gross margins for the producer were assumed, T&R was preferred at all prevalence rates. Vaccination was preferred when lower gross margins, lower vaccination costs, or better protective effect of PRV vaccines on reproductive performance were assumed. The use of gene-deleted vaccines in conjunction with the T&R strategy was also evaluated. When this option was available, T&R was favored at any prevalence rate (T&R alone when the prevalence was less than or equal to 20%, or combined with gene-deleted vaccination at prevalences greater than 20%). Depopulation-repopulation was not the best option under any circumstance. Once formulated, a decision-tree analysis can be adapted to the prevailing economic or epidemiologic conditions; hence, it is a useful tool in the PRV decision-making process.  相似文献   

18.
Thirty-eight grossly and histologically normal cat kidneys were examined ultrasonographically. The echogenicity of the renal cortex was subjectively evaluated by scoring it as largely or not largely different from the echogenicity of the renal medulla and as similar or not similar to the echogenicity of the renal sinus. The presence or absence of a medullary hyperechoic band was determined. The length, width, and height of each kidney was measured. Hematoxylin and eosin-stained sections of each kidney were examined microscopically. The amount of fat vacuoles in the tubular epithelium of the renal cortex was scored as plentiful or not plentiful. The presence or absence of a medullary band of mineral deposits within the lumina of renal tubules was determined. A plentiful amount of fat vacuoles in renal cortex was associated positively with a large difference in echogenicity between cortex and medulla (P less than 0.01) and with similar echogenicity of cortex and sinus (P less than 0.01). The presence of a medullary hyperechoic band was associated positively with a band of mineral deposits within medullary tubular lumen (P 0.01). Kidneys with a large difference in echogenicity between cortex and medulla and kidneys with a plentiful amount of fat vacuoles were not significantly different in size (P = 0.56). These groups were larger (P less than 0.01) in length, width, and height than were kidneys without a large difference in echogenicity between cortex and medulla and kidneys that did not have plentiful cortical fat vacuoles.  相似文献   

19.
Treatment of dermatophytosis in dogs and cats: review of published studies   总被引:7,自引:2,他引:5  
The recent literature on the treatment of dermatophytosis in dogs and cats was reviewed. Based upon in vitro studies using isolated infected hairs and controlled or field in vivo studies, the following topical treatments were consistently found to be antifungal (i.e. antidermatophyte): lime sulfur (1:16), 0.2% enilconazole rinses, and a combined 2% miconazole/chlorhexidine shampoo. Animals or hairs were either bathed or rinsed once or twice weekly. Itraconazole, griseofulvin and terbinafine were evaluated in controlled or field studies, most commonly involving cats. Griseofulvin (50 mg kg(-1)) was reported to cure infected animals in 41-70 days. Itraconazole (10 mg kg(-1) once daily or in a combined daily/pulse therapy 10 mg kg(-1) once daily for 28 days and then week on/week off) was reported to cure infected animals in 56-70 days. Low-dose itraconazole (1.5-3.0 mg kg(-1)) in 15-day cycles required 1-3 cycles (15-45 days). Various doses of terbinafine (5-40 mg kg(-1)) were reportedly used to treat dogs or cats. The higher doses of terbinafine (> 20 mg kg(-1)) were required to achieve a mycological cure; the number of treatment days to cure varied from 21 to > 126 days. Lufenuron was reported anecdotally to be an effective cure, however, this was not substantiated in controlled studies. Finally, fungal vaccines were not found to be effective against challenge exposure, however, there is evidence that they may be useful in treatment protocols.  相似文献   

20.
The accuracy of diagnoses based on examination of frozen sections was determined by comparing the results to those obtained by examination of tissues prepared using conventional methods (formalin fixation, paraffin-embedded tissue). One hundred ninety-four specimens were examined using the frozen section technique; 37 were examined to confirm a tentative diagnosis or to document lymph node metastasis and the remainder were examined to diagnose an unknown pathologic process. Of the 194 specimens examined, an accurate, specific diagnosis was obtained in 161 (83%); in 19 (10%), the pathologic process was correctly identified, but a specific diagnosis was not obtained; and in 2 (1%) the diagnosis was deferred. The remaining 12 (6%) were incorrectly diagnosed by the frozen section technique. When the number of specimens in which a specific diagnosis was obtained was combined with the number of specimens in which the pathologic process was correctly identified, the overall accuracy rate of the frozen section technique was 93%. There was no difference in the accuracy of the frozen section technique based on the reason for submission of the sample, source of tissue submitted, or the type of pathologic process (i.e., inflammatory or neoplastic). Of the 12 incorrect diagnoses, 4 (33%) were because of sampling errors and 8 (67%) were caused by interpretation errors. The proposed indications for the use of intraoperative frozen sections are: 1) to determine the nature of a pathologic process for which a preoperative diagnosis has not been established, 2) to determine the extent of spread of neoplastic tissue to lymph nodes and other organs, 3) to evaluate resection margins of a neoplastic process, and 4) to clarify situations where a discrepancy exists between the preoperative cytologic or histologic diagnosis and intraoperative gross pathology.  相似文献   

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