共查询到18条相似文献，搜索用时 656 毫秒
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采用AFLP分子标记技术对山东省30个枣栽培品种进行了遗传多样性分析。从64对引物组合中,筛选出重复性好、多态性高的引物组合8对,共扩增出1178条条带,其中多态性条带1148条,多态性比率97.45%,表明供试枣品种间具有丰富的遗传多样性。应用NTSYS软件计算样本间的遗传距离,NJ法进行聚类分析结果表明,不同品种间的遗传距离在0.2935～0.7794,平均为0.5663;鉴定了近年来选育的傲雪、乐金4号、沾冬2号与原有品种不同,是枣新品种;并将枣早熟品种、晚熟品种和抗裂果品种分别进行了聚类。AFLP研究较好地揭示了枣种质资源间亲缘关系,为枣品种鉴定、进一步开展枣早熟、晚熟和抗裂果基因研究奠定了基础。 相似文献
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菠萝种质鉴定及亲缘关系的AFLP分析 总被引：3，自引：1，他引：2
从FISH-AFLP试剂盒(PSTI/MseI型)64对引物中,筛选出8对AFLP选择性引物P-GAA/M-CTA、P-GAC/M-CTG、P-GAC/M-CTT、P-GAG/M-CAT、P-GAG/M-CTA、P-GAT/M-CAT、P-GAT/M-CTA、P-GAT/M-CTC,对39份菠萝种质进行了AFLP分析,都得到了清晰的多态性指纹图谱。8对引物共获得454个遗传位点,其中多态性位点332个,多态性比例平均为73.1%。UPGMA聚类表明,各种质间的相似系数为0.73～0.98,说明39份菠萝种质遗传关系相对较近。依相似系数0.80的水平,将供试的39份菠萝种质分为4类。研究结果可以作为菠萝分类的理论依据和基础。 相似文献
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陈徵尼仲玲玲邹天福戚建莉 《中国果树》2015,(1):18-21
利用ISSR分子标记技术对甘肃省42份枣种质资源的遗传多样性进行分析。结果表明:筛选出8条引物共扩增出76条DNA谱带,其中多态性谱带70条,多态性位点比率92.0%;按照42份枣种质间的遗传距离构建UPGMA聚类图,42份枣种质被划分为4个大类、10个亚类,遗传多样性处于较高水平;从聚类结果看,原产自甘肃省的枣品种遗传背景相似,主要根据原产地进行聚类,目的性品种交换现象较少。另外,对6个未知身份的枣品种进行了初步鉴定。 相似文献
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菊花品种资源遗传多样性的AFLP分析 总被引：13，自引：0，他引：13
以AFLP—银染分子标记技术, 对45个菊花品种进行了遗传多样性和亲缘关系分析。选用10个多态性高、分辨力强的E + 3 /M + 3引物组合分别对供试材料的基因组DNA进行扩增, 共获得486条清晰可辨的条带, 其中多态性带451条, 平均每个引物组合可检测出4511个多态性位点, 多态性位点百分率高达92.80% , 这表明供试品种资源在DNA水平上酶切位点的分布存在广泛的变异。应用DPS软件计算供试品种遗传距离界于0.36000～0.86237之间, 平均为0.611185; UPGMA分析将45份资源分成6个类群,从相异性系数分析了各品种资源间的亲缘关系。 相似文献
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利用AFLP鉴定柑橘变异 总被引：4，自引：2，他引：4
利用AFLP分析鉴定了7个柑橘变异类型,从64对引物组合中筛选了8对引物组合,共得到334个扩增位点2099条带数据。7个变异类型与亲本品种之间多态性带为36至92条,变异类型的多态性带数与亲本品种总带数的比例为0.1721至0.4182。说明变异类型的遗传基础已经发生了不同程度的改变,7个柑橘变异类型应是芽变,具备了成为新品种的遗传基础,特别是红皮冰糖脐橙选育成功将填补高糖脐橙空白。研究同时表明,AFLP具有扩增谱带多,谱带清晰,扩增信息量大的特点。根据谱带位点差异即可鉴定柑橘变异是否遗传,多态性位点数也反映了性状变异程度。AFLP方法1次可同时鉴定多个变异类型,是柑橘新品种选育早期鉴定灵敏、可靠、有效的方法。 相似文献
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梨新品种及其亲本的AFLP分析 总被引：12，自引：0，他引：12
利用荧光AFLP技术, 对20个梨新品种及其23个亲本(共43个品种) 进行研究。从64对引物组合中筛选出7对用于扩增, 共获得784条带, 其中多态性带699条, 多态性为89.2%。扩增结果显示, 7对引物组合在29个品种中扩增出特征带, 每对引物组合均能将所有品种鉴别开, 表明荧光AFLP技术用于梨品种鉴定的效率很高。通过聚类, 从分子水平对梨新品种及其亲本的遗传关系进行分析, 并对20个梨新品种进行分类, 为梨杂交育种的亲本选配提供了理论参考。 相似文献
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适合我国南方地区栽培的枣优良品种亲缘关系研究 总被引：4，自引：0，他引：4
利用荧光AFLP分子标记技术,对适合我国南方地区栽培的26个枣优良品种亲缘关系进行研究。结果表明,8对引物共扩增得到886条带,其中多态性条带为817条,多态性比例达到了92.2%,体现了非常丰富的遗传多样性。系统聚类结果表明大部分来源于北方地区的鲜食品种聚为一类,彼此间亲缘关系较近;制干、兼用品种和南方本地品种表现出较近的亲缘关系。酥脆枣与孔府酥脆枣亲缘关系很近,而河南鸡蛋枣与湖南鸡蛋枣之间存在着较远的遗传距离。 相似文献
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Gladiolus is one of the important commercial flowers with a large number of cultivars. However, genetic relationships among its genotypes have not been reported. This study analyzed genetic relatedness of 54 gladiolus cultivars using amplified fragment length polymorphism (AFLP) markers. A total of 24 AFLP primer pairs with three samples were initially screened, from which 9 primer sets that showed clear scorable and highly polymorphic bands were selected for AFLP reactions. Fluorescence-labeled amplification products were subjected to electrophoresis and then analyzed using an automated sequencer. A dendrogram was constructed by the unweighted pair group method using the arithmetic average (UPGMA). The number of AFLP fragments generated per primer set ranged from 10 to 151 with fragment sizes varying from 50 to 450 bp. A total of 660 AFLP fragments were detected, of which 658 (99.70%) were polymorphic. All the primers except E-AGG/M-CTA displayed 100% polymorphism. All cultivars were clearly differentiated by their AFLP profiles. The AFLP data were compared with previously obtained RAPD data and combined to generate a common dendrogram. The first cluster was dominated with indigenously bred cultivars while the second was dominated with exotic cultivars. This shows that most of the exotic cultivars as well as indigenous cultivars are closely related with each other. However, two indigenous cultivars viz., Pusa Suhagin and Pusa Archana share genetic similarity with exotic cultivars. Among the genotypes selected for the investigation, Pusa Gunjan was identified as the most distinct genotype. The AFLP markers developed will help future Gladiolus cultivar identification, germplasm conservation and new cultivar development. The assessed genetic relationships among gladiolus cultivars may enhance the efficiency of breeding program by selecting desirable parents with reduced breeding cycle. 相似文献
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扁桃种质资源的AFLP分析 总被引：10，自引：1，他引：10
利用AFLP分子标记技术对国内外49份扁桃材料的亲缘关系进行了鉴定,使用3对选择性引物组合,每对引物扩增出136条带,并对其进行聚类分析。结果表明,不同种以及不同品种间的遗传距离不同。在相似系数小于0.68时,大多数栽培品种聚为一类,并可将野生扁桃组、苦巴旦组、榆叶梅,以及桃、中国樱桃、欧洲甜樱桃分开。栽培品种中,同一种源区的大多数栽培品种能聚类在一起。我国的扁桃资源与国外的扁桃资源遗传差异较大。 相似文献
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J. Fang C. Wang H. Yu X. Li C. Song J. Chen 《The Journal of Horticultural Science and Biotechnology》2013,88(6):585-590
SummarySweet orange (Citrus sinensis) represents an important group of Citrus fruit; however, the identification of sweet orange cultivars during vegetative growth can be difficult. A study on the genetic identification of sweet orange cultivars may be significant for the sweet orange nursery industry, for cultivar-rights protection, and is important for the genetic evaluation and conservation of these orange cultivars. In this study, amplified fragment length polymorphism (AFLP) markers were used to genotype 57 sweet orange cultivars. Ten PCR primer pairs generated 629 unique AFLP bands, with a size range of 50 ? 500 bp. Seventy-four bands (11.8%) were polymorphic. On average, each primer pair produced 62.9 fragments, with 7.4 polymorphic fragments. A dendrogram of the 57 sweet orange cultivars was constructed based on an UPGMA analysis using Jaccard?s coefficients of similarity. This provided a clear comparison of the genetic variation between cultivars and an ability to identify them. From Jaccard?s coefficients, 56 of the 57 cultivars examined were genetically close, with coefficient values ≥ 0.985. ?Variegated Navel? was less closely-related, with a much lower coefficient value (0.94). Among the 57 cultivars, 28 sub-groups, some consisting of only one cultivar, could be separated by their AFLP fingerprints. Compared to ISSR and SSR markers, AFLP seemed to be the preferential marker technique for the identification of sweet orange cultivars. 相似文献
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杧果主要品种遗传多态性的AFLP标记研究 总被引：13，自引：0，他引：13
采用扩增片段长度多态性AFLP标记对国内外31个杧果主要品种进行分类与亲缘关系研究。结果表明: 筛选出的14对引物组合在31份杧果种质中共扩增出了1 761条带, 其中多态性带的比例为97%。平均每对引物产生125.8条带和121.6条多态性带, 总的多态性带率为97%; 基于AFLP标记, 以0.48的相似系数为阈值, 所有供试 果材料被分为7大组群, 第一组群内以0.52为阈值, 又可分为6组。与形态标记相比, 基于AFLP标记的杧果分类体系更能反映杧果品种间的亲缘关系。 相似文献