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用患病雏鹅的肝、脾、肾作病料分离病毒,经临床诊断和实验室诊断,证明了分离病毒为鹅细小病毒和鹅病毒性肠炎病毒两种病毒,并用此两种病毒研制成油乳剂灭活苗,结果表明该油乳剂灭活苗对小鹅瘟和鹅病毒性肠炎的预防和治疗效果是十分理想的. 相似文献
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根据雏鹅新型病毒性肠炎病毒(NGVEV)保守基因序列,设计合成一对引物并且进行PCR检测,扩增出预期的223 bp条带。该方法能在雏鹅新型病毒性肠炎病毒BC07株中扩增到特异性片段,而鹅细小病毒、鸭瘟病毒、鹅副黏病毒的扩增结果均为阴性;敏感性试验表明该方法最低检出限量为2.5×10~(-1)EID_(50)。表明所建立的PCR方法具有敏感性高、特异性强的特点。可以用来检测雏鹅新型病毒性肠炎。 相似文献
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本试验旨在研制小鹅瘟和雏鹅新型病毒性肠炎二联PCR检测试剂盒,观察临床应用效果。根据小鹅瘟和鹅病毒性肠炎基因序列,设计合成一对特异性引物,对PCR反应条件进行优化,并分别对试剂盒的特异性、敏感性、重复性和保存期进行研究,之后进行临床扩大试验。该二联PCR诊断试剂盒能扩增出预期的小鹅瘟375 bp核酸片段和鹅病毒性肠炎223 bp核酸片段,与鸭瘟病毒、鹅副黏病毒无交叉反应;最低能检测到小鹅瘟病毒104 ELD50/mL,雏鹅新型病毒性肠炎病毒2.5×10-1ELD50;不同批次的试剂盒对同一样品检测结果一致;常温下至少可保存5个月;临床应用效果显著。该二联PCR检测试剂盒能够对小鹅瘟和雏鹅病毒性肠炎早期诊断、流行病学调查和卫生检疫提供了检测手段。 相似文献
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雏鹅新型病毒性肠炎于1997年首次被发现,是雏鹅的一种卡他性、出血性、纤维性渗出及坏死性肠炎,其临床症状和病理变化与小鹅瘟非常相似。通过对该病的病毒分离、鉴定、病原学特性的研究,证明了雏鹅新型病毒性肠是由雏鹅的一种新的腺病毒引起的、不同于小鹅瘟的新传染病。1流行特点 相似文献
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雏鹅新型病毒性肠炎的防制 总被引：1，自引：0，他引：1
《养禽与禽病防治》2004,(10)
雏鹅新型病毒性肠炎于1997年首次被发现,是雏鹅的一种卡他性、出血性、纤维性渗出及坏死性肠炎,其临床症状和病理变化与小鹅瘟非常相似。通过对该病的病毒分离、鉴定、病原学特性的研究,证明了雏鹅新型病毒性肠是由雏鹅的一种新的腺病毒引起的、不同于小鹅瘟的新传染病。一、流 相似文献
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雏鹅新型病毒性肠炎病毒(NGVEV)是一种能引起30日龄以内雏鹅感染发病的病毒,其流行病学、临床症状、病理变化与小鹅瘟(GP)相似,因而,在实际生产中,常将其误诊为GP.种鹅在开产前注射1次GP疫苗,但其子代于3日龄仍出现发病和死亡情况,用GP高免血清治疗无效.后经病毒分离、人工感染雏鹅、电镜观察病毒粒子、免疫接种和治疗等方法,将该种现象确诊为是由腺病毒引起的一种尚未被认识的新型病毒性肠炎. 相似文献
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雏鹅新型病毒性肠炎,是由雏鹅新型病毒性肠炎病毒引起的3～30日龄雏鹅的一种卡他性、出血性、纤维素性和坏死性肠炎。以昏睡、腹泻、消瘦、喙端色暗为主要临床症状。其病理特征为小肠粘膜的出血和形成凝固性栓塞物阻塞肠腔。 相似文献
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Comparison of a modified counterimmunoelectrophoresis test and a microimmunodiffusion test for detection of pseudorabies virus antibodies in porcine sera. 
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下载免费PDF全文 P P Williams M F Coria E C Pirtle G A Erickson 《Canadian journal of veterinary research》1984,48(1):92-96
The correlation of a modified counterimmunoelectrophoresis (CIE) test and a microimmunodiffusion test for detecting pseudorabies virus antibodies in porcine sera was investigated, using as reference a standard virus neutralization test. The counterimmunoelectrophoresis test exhibited a sensitivity comparable to the microimmunodiffusion test but was not as sensitive as the virus neutralization test. The best feature of the modified counterimmunoelectrophoresis test is that it is a rapid test. It provides an alternative to currently used diagnostic tests for detection of pseudorabies virus antibodies in sera from field reared and experimentally reared swine exposed to pseudorabies virus. 相似文献
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A plaquing system and plaque neutralization test in porcine thyroid cells were used to study different transmissible gastroenteritis isolates and hemagglutinating encephalomyelitis virus. Among transmissible gastroenteritis virus isolates, plaque size varied considerably and mixed size ranges sometimes occurred. The most recently isolated viruses produced smaller plaques than the laboratory viruses or hemagglutinating encephalomyelitis virus. All transmissible gastroenteritis virus isolates reacted in the plaque neutralization test with a transmissible gastroenteritis virus antiserum which showed no activity against hemagglutinating encephalomyelitis virus. Plaque neutralization results both from experimentally infected pigs and following a field outbreak demonstrated the reliability of this test and its greater sensitivity than the conventional tube test. 相似文献
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B M Adair 《Research in veterinary science》1986,41(3):414-416
The fluorescent antibody (FA) test is compared with the haemagglutination inhibition (HI) test for parainfluenza virus type 3 (PI-3) and virus neutralisation (VN) test for respiratory syncytial (RS) virus for detection and titration of virus-specific antibodies. In experimentally inoculated calves PI-3 and RS virus FA tests detected seroconversion at the same time as HI and VN tests, however, in serially diluted sera, the FA test was positive to higher dilution. In studies with paired samples from calves from four farms with respiratory problems, the FA test gave similar results to PI-3 HI and RS virus VN tests. Large increases in antibody titre to RS virus detected by FA and VN tests indicated this was the problem on two of the farms. Individual animals showed large rises to PI-3 by FA and HI test on three farms. It is concluded that the FA test provides a rapid and sensitive alternative to the more conventional serological tests for respiratory viruses. 相似文献
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Indirect haemagglutination test for the detection and assay of antibody to bovine respiratory syncytial virus 总被引：3，自引：0，他引：3
H T Martin 《The Veterinary record》1983,113(13):290-293
An indirect haemagglutination (IHA) test was used for the rapid assay of antibody to bovine respiratory syncytial virus. Antigens for the sensitisation of formalised tanned erythrocytes were prepared by treatment of virus infected cells with non-ionic detergent. A close serological relationship was shown by the IHA test between the strain of bovine respiratory syncytial virus used and the A2 strain of human respiratory syncytial virus. The IHA test was sensitive and reproducible. A linear correlation was demonstrated between antibody titres obtained by the IHA test and the serum neutralisation test. Titres obtained by the IHA test were approximately 60 times greater than serum neutralisation titres. Serum samples from 803 two-year-old heifers in 48 herds in England were examined by the IHA test. Ninety-four per cent of the animals had antibody to respiratory syncytial virus. Examination of paired serum samples from outbreaks of respiratory disease by the IHA test showed that respiratory syncytial virus was associated with seven out of 15 outbreaks. 相似文献
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为制备外源病毒检验用抗鸡传染性法氏囊病病毒(Infectious Bursal Disease Virus,IBDV)的特异性血清,对300只3~4周龄的SPF鸡进行了基础免疫和加强免疫。最后一次免疫后21d采血并分离血清,血清经混合、分装、冷冻真空干燥后,对其进行了无菌检验、外源病毒检验、剩余水分测定、中和效价测定和特异性检验。结果表明,本研究制备的抗IBDV特异性血清无菌检验、外源病毒检验和剩余水分测定均符合《中华人民共和国兽药典》三部(二〇一〇年版)规定;血清中和效价为1:5747,与IBDV B87株毒种中和指数为104.3,与鸡新城疫病毒La Sota株、鸡传染性支气管炎病毒H120株、鸡传染性支气管炎病毒H52株、鸡传染性喉气管炎病毒、鸡痘病毒、禽呼肠孤病毒S1133株的中和指数均不大于10,通过AGP、ELISA、HI等试验确定血清中不含其他禽源外源病毒抗体。本研究为鸡传染性法氏囊病活疫苗或毒种的外源病毒检验提供了具有良好特异性和高效价的中和用血清。 相似文献