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《园艺学报》2020,(5)
以龙眼‘蜀冠’ב大乌圆’的F1代65个单株为材料,对其果实的27个品质性状进行了连续3年的观测,应用SPSS19.0软件对各性状进行变异系数、Shannon-Weinner多样性指数和主成分分析。结果表明:65个单株果实品质性状存在着极显著差异,13个数量性状(单果质量、果实纵径、果实横径、可溶性固形物、可食率、果皮厚度、果皮质量、果肉厚、种子质量、维生素C含量、蔗糖含量、葡萄糖含量和果糖含量)变异系数在6.95%～41.64%之间;多样性指数在1.33～2.02之间;14个描述性状(成熟期、果皮颜色、果形、果肩、果顶、龟裂纹、疣状突起、果肉颜色、果肉质地、果肉透明度、果肉离核难易度、果汁、化渣程度和果肉风味)多样性指数在0.56～1.28之间;主成分分析发现前5个性状(单果质量、可食率、可溶性固形物、成熟期和果实风味)的累积贡献率为87.50%;建立了‘蜀冠’ב大乌圆’杂交后代果实品质性状综合评价的方法,利用该方法在65个杂交后代中筛选出了5个优异的单株(SD7、SD11、SD36、SD53和SD120),结果与实际观测结果高度一致。 相似文献
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以杧果‘金煌’ב热农1号’的F1代54个单株为材料,对其果实性状进行观测,并对单果质量等9个数量性状进行了遗传分析,对30个数量性状和描述性状进行了Shannon-Weaver多样性和主成分分析。结果表明,9个数量性状（单果质量、果实纵径、横径、侧径、果形指数、可溶性固形物、可食率、果核长度和果核质量）在后代中的遗传符合正态或偏正态分布,变异系数在7.98%～41.73%之间,多样性指数在1.65～2.04之间,遗传传递力在84.74%～117.00%之间,果实大小等性状呈趋中变异趋势,可溶性固形物呈超亲遗传趋势。21个描述性状的多样性指数在0.16～1.48之间。主成分分析发现前10个主成分的累积贡献率为75.19%,并筛选出单果质量、抗性、果形指数、果皮光滑度、果肉纤维长度、果核表面特征、果实风味、可溶性固形物、胚类型和果肉颜色作为评价杂交后代果实的主要性状。基于观测数据建立了杧果杂交后代果实性状综合评价模型,并利用该方法结合田间观察和实际观测结果筛选出8个优异的单株。 相似文献
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【目的】探究杏果实主要性状的遗传倾向以及其在正、反交后代中的遗传差异,为杏育种过程中的父母本选配以及后代性状表现的预测提供依据。【方法】对‘串枝红’与‘赛买提’正、反交组合的单果质量、果面盖色、果实形状、果肉硬度和可溶性固形物含量等性状进行了调查,每株杂交后代调查10个果实。【结果】正、反交后代果实大小(单果质量与果实纵、横、侧径)均有偏小的遗传倾向;果面盖色属于质量性状,有彩色相对于无色为显性;果实形状(果实外观形状、纵径/横径、侧径/横径)果实形状表现为趋圆的遗传倾向;正、反交后代果肉硬度与可溶性固形物含量均呈偏低遗传的倾向;果实风味表现为偏酸的遗传倾向。【结论】‘串枝红’与‘赛买提’正、反交后代单果质量、纵、横、侧径多为累加效应形成,且正、反交组合果实累加效应相似,均完全解体;两组合可溶性固形物虽然存在加性效应数量性状的解体,但在子代中由于新的累加效应形成,后代出现超高亲株系。 相似文献
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黄凤珠陆贵锋武志江邓海燕黄黎芳梁桂东李祯英 《中国南方果树》2019,(6):46-52
对农业部南宁火龙果种质资源圃内不同来源地的218份火龙果种质的果实形状、果皮果肉颜色、果实大小、可溶性固形物、可食率、果肉质地和风味等8个果实品质性状进行了鉴定评价和分析。结果表明,供试种质果实形状从扁圆形到长椭圆形,果皮颜色从玫红色到暗紫红色,果肉颜色从白色到深紫红色,果肉质地从软绵到较粗、从细滑到较紧实,果肉风味从较淡到蜜甜、从甜酸到微酸,单果质量89.62~554.83g,果肉中心部位可溶性固形物含量10.7%~22.5%,果实可食率50.97%~81.54%,果实品质评价等级从较差到优良均有分布。果实的单果质量、果肉中心部位可溶性固形物、果实可食率3个数量性状的变异系数和多样性指数(H′)分别为36.89%、9.21%、8.23%和2.0391、2.0397、1.9797,火龙果种质资源之间果实品质性状表现出不同变异和多样性。 相似文献
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火龙果种质资源果实品质性状多样性分析 总被引：1，自引：0，他引：1
《中国南方果树》2019,(6)
对农业部南宁火龙果种质资源圃内不同来源地的218份火龙果种质的果实形状、果皮果肉颜色、果实大小、可溶性固形物、可食率、果肉质地和风味等8个果实品质性状进行了鉴定评价和分析。结果表明,供试种质果实形状从扁圆形到长椭圆形,果皮颜色从玫红色到暗紫红色,果肉颜色从白色到深紫红色,果肉质地从软绵到较粗、从细滑到较紧实,果肉风味从较淡到蜜甜、从甜酸到微酸,单果质量89.62～554.83 g,果肉中心部位可溶性固形物含量10.7%～22.5%,果实可食率50.97%～81.54%,果实品质评价等级从较差到优良均有分布。果实的单果质量、果肉中心部位可溶性固形物、果实可食率3个数量性状的变异系数和多样性指数(H′)分别为36.89%、9.21%、8.23%和2.039 1、2.039 7、1.979 7,火龙果种质资源之间果实品质性状表现出不同变异和多样性。 相似文献
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GONG Qing-juan WANG Hong-hua LIANG Ying LU Zhen-he CHEN Jin-sheng HUANG Qiao-dong YUE Yu 《园艺学报》2015,31(5):834-838
AIM: To investigate the effects of P2X4 receptor on peri-sciatic administration of recombinant rat TNF-α (rrTNF)-induced mechanical allodynia. METHODS: Male Sprague-Dawley rats (180~200 g) were used in the experiments. The levels of P2X4 receptor on day 3, day 7 and day 14 after peri-sciatic administration of rrTNF were examined by Western blot, and the location of P2X4 receptor in the spinal dorsal horn was observed by double immunofluorescence staining. The changes of 50% paw-withdrawal thresholds of the rat were detected by behavioral test, and the level of TNF-α in the spinal dorsal horn was also examined by Western blot when TNP-ATP was intrathecally injected before the administration of rrTNF. RESULTS: Compared with control group, the expression of P2X4 receptor in the spinal dorsal horn on the ipsilateral side significantly increased on day 3, day 7 and day 14 (P<0.01) after rrTNF (100 ng/L) administration. P2X4 receptor was co-localized only with microglia, but not with neurons or astrocytes. Intrathecal injection of TNP-ATP before rrTNF administration prevented mechanical allodynia induced by rrTNF and inhibited the upregulation of TNF-α in the spinal dorsal horn. CONCLUSION: P2X4 receptors in microglia may be involved in rrTNF-induced mechanical allodynia by the upregulation of TNF-α in the spinal dorsal horn. 相似文献
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AIM: To investigate the effects of Src family kinases (SFKs) on adenosine 5'-triphosphate (ATP)-induced long-term potentiation (LTP) in the spinal dorsal horn. METHODS: Male Sprague-Dawley rats (250-280 g) were used in the experiments. Western blotting, electrophysiological recording in spinal dorsal horn in vivo and immunohistochemistry were used in the study. The C-fiber-evoked field potentials were recorded at the superficial layers of spinal dorsal horn at the lumbar enlargement and the phosphorylation level and location of SFKs in spinal dorsal horn were examined by Western blotting and immunohistochemistry. RESULTS: Thirty min and 60 min after ATP application, the levels of phosphorylated SFKs (p-SFKs) were significantly increased.The p-SFKs were expressed in microglia, but not in astrocytes or neurons. Spinal application of SFK inhibitors prevented ATP-induced LTP. CONCLUSION: Microglial SFKs may play an important role in ATP-induced LTP of C-fiber evoked field potentials in the spinal dorsal horn. 相似文献
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AIM：To investigate the neuronal nuclear antigen (NeuN) and neuron-specific enolase (NSE) distribution in the developing stage of human embryonic small intestines and their clinical significance. METHODS：Sixteen cases of human embryos at 2~4 months of gestational age were used in the present study. The technique of immunohistochemical staining was used to detect the expression and distribution of NeuN and NSE in small intestinal walls. RESULTS：At 2~4 months of gestational age, NSE was strongly expressed in neurons and nerve fibers of the small intestinal myenteric nerve plexuses in human embryos. The numbers of NSE-positive cells and fibers gradually increased in the small intestinal submucosa with the increase in gestational age, and a few NSE-positive cells located in the small intestinal glands of human embryos. NeuN-positive cells scattered in the epithelium and glands of the small intestinal mucosa, and the number of NeuN-positive cells gradually increased with the increase in gestational age. But there were no NeuN-positive cells in the small intestinal submucosa. CONCLUSION: The expression and distribution of NeuN are not consistent with those of NSE during the development of human embryonic small intestines, and both of them may be involved in the development of neurons and neuroendocrine cells in small intestinal walls. 相似文献
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AIM: To study the effects of protein kinase C (PKC) inhibitor， chelerythrine chloride (CH)， on nociceptive response, nitric oxide synthase (NOS) expression and nitric oxide (NO) content in spinal cord of rats with inflammatory pain. METHODS: Inflammatory pain was induced by formalin injection into right hind paw. NADPH-d histochemistry was used to investigate the changes of NOS expression. Nitrate/nitrite (NO2-/NO3-) was assayed to represent NO content. RESULTS: Compared with the control group, the number of NADPH-d positive cells increased significantly in the superficial layer (LaminaeⅠ-Ⅱ) of the spinal cord dorsal horn and the grey matter surrounding the central canal (Laminae Ⅹ) in rats with inflammatory pain, the reactive degree of NADPH-d positive soma and fibers and NO content of the lumbar enlargement of spinal cord also increased significantly. Intrathecal injection of CH inhibited the spontaneous pain response in the second phase induced by formalin injection, and prevented the increases in the number and reactive degree of NADPH-d positive cells, as well as NO content of the lumbar enlargement of spinal cord. CONCLUSION: It is suggested that the activation of PKC promotes NOS expression and NO production in the nociceptive neurons of spinal cord during formalin-induced inflammatory pain. 相似文献
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YANG Hong-wei ZHANG Hong-mei HU Xiao-dong XIN Wen-jun ZHANG Tong ZHOU Li-jun LIU Xian-guo 《园艺学报》2004,20(4):513-517
AIM: The role of cyclic adenosine monophosphate (cAMP)-dependent protein kinase (PKA) in the induction and maintenance of spinal long-term potentiation (LTP) was evaluated. METHODS: The C-fiber evoked field potentials were recorded at the superficial layers of spinal dorsal horn at the lumbar enlargement. RESULTS: (1) 8-Br-cAMP induced LTP of C-fiber evoked field potentials and 8-Br-cAMP-induced LTP occludes tetanus-induced LTP. (2) Rp-CPT-cAMPS, an inhibitor of PKA, blocked the induction of spinal LTP and reversed established LTP of C-fiber evoked field potentials in a time-dependent manner. (3) In the presence of anisomycin, a protein synthesis inhibitor, the potentiation induced by 8-Br-cAMP was completely blocked. (4) PD98059, a selective inhibitor of mitogen-activated protein kinase (MAPK),completely blocked the 8-Br-cAMP-induced LTP.CONCLUSION: Activation of PKA signal pathway in spinal dorsal horn may be crucial for the induction and the early-phase maintenance of LTP of C-fiber evoked field potentials. 相似文献
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AIM: To observe the change of heme oxygenase(HO-1)expression in the spinal cord during formaldehyde-induced pathological pain and investigate the effect of HO-1 on neuronal apoptosis in the spinal cord induced by formaldehyde inflammatory pain.METHODS: The protein expression of HO-1 in the left and the right spinal dorsal horn was detected by Western blotting. The neuronal apoptosis rate of the spinal cord was determined by flow cytometry.RESULTS: Compared to control group, the protein expression of HO-1 was significantly up-regulated at different time points after the injection of formaldehyde, which was most obviously 24 h after the injection of formaldehyde and was still higher than that in control group at 72 h. Compared to control group, the neuronal apoptosis rate of spinal cord increased in the rats with formaldehyde inflammatory pain. No significant difference of the neuronal apoptosis rate was observed between formaldehyde group and solvent control group . Intrathecal injection of 100 μg ZnppIX, an inhibitor of HO-1, attenuated the degree of spontaneous pain response, but induced an increase in the rate of neuronal apoptosis in spinal cord in the rats with formaldehyde inflammatory pain. CONCLUSION: Formaldehyde inflammatory pain induces the increases in HO-1 expression and neuronal apoptosis in the rat spinal cord. HO-1 promotes the response of spontaneous pain and inhibits the process of neuronal apoptosis in spinal cord induced by formaldehyde inflammatory pain. 相似文献
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WEN Xian-jie LIANG Hua YANG Cheng-xiang LI Heng LIU Hong-zhen ZENG Yin-ming LI Zhang-jun 《园艺学报》2008,24(7):1351-1355
AIM: To investigate the effect of mibefradil administered intrathecally on the pain threshold and the expression of neuron nitric oxide synthase (nNOS) in the spinal cord of the rats following chronic compression of dorsal root ganglion. METHODS: The chronic compression of dorsal root ganglion (CCD) model was adopted. Three different doses of mibefradil, a T-type calcium channel blocker, were administered singly intrathecally to the rats after CCD 5 days. The mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) were determined with von Frey hair and thermal radiation apparatus before mibefradil administered and after mibefradil administered 30 min, 60 min, 120 min, 240 min, and 480 min. Changes of nNOS expression in neurons of the spinal dorsal horn were detected by immunohistochemistry method, and the number of the nNOS positive neurons was counted under microscope. RESULTS: MWT and TWL declined significantly from the first day to the 21st day after CCD. Mibefradil administered intrathecally attenuated significantly both mechanical and thermal hyperagesia of the rats, and markedly inhibited the increase in the nNOS expression. CONCLUSION: These data show that T type calcium channel plays a key role in the CCD-induced mechanical and thermal hyperalgesia and possibly involves in the nNOS expression in the spinal. 相似文献
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YANG Hong-wei ZHANG Hong-mei HU Xiao-dong XIN Wen-jun ZHANG Tong ZHOU Li-jun LIU Xian-guo 《园艺学报》2005,21(2):252-255
AIM: The role of protein kinase C (PKC) in the induction and maintenance of spinal long-term potentiation (LTP) was evaluated. METHODS: The C-fiber evoked field potentials were recorded at the superficial layers of spinal dorsal horn at the lumbar enlargement. RESULTS: (i) Chelerythrine （200 μmol/L） or G 6983 (100 μmol/L), a selective PKC inhibitor, completely blocked LTP induction. (ii) Chelerythrine or G 6983 reversed spinal LTP in a time-dependent manner. 15 min after LTP induction, chelerythrine （200 μmol/L） and G 6983 (100 μmol/L) depressed LTP to baseline in all tested rats. The same concentration of chelerythrine and G 6983, applied at 3 h after LTP induction, did not affect LTP. CONCLUSION: PKC in spinal dorsal horn may be crucial for the induction and the early-phase maintenance of LTP of C-fiber evoked field potentials. 相似文献
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AIM:To investigate the role of Chinese medical herb Radix Puerariae extract puerarin in a rat model of radicular pain caused by lumbar disc herniation (RAPLDH) and to explore the possible mechanism involving spinal glial cell activation and inflammatory response. METHODS:The rat model of RAPLDH was induced by autologous nucleus pulposus (NP) implantation. The rats in sham group received the same operation procedure except NP implantation. Puerarin injection at different doses (50, 100 and 150 mg/kg) was delivered intraperitoneally 1 h before surgery, and once daily for 7 d. Mechanical paw withdrawal threshold (PWT) test was employed for assessing pain behaviors. Spinal microglia and astrocyte activation was evaluated by immunofluorescence staining of relevant specific markers. The expression of pro-and anti-inflammatory cytokines in spinal dorsal horn was measured by ELISA. RESULTS:The rats with NP implantation showed long-lasting pain behaviors, characterized by decrease in PWT from day 3 to day 14 after surgery. Compared with vehicle group, puerarin at doses of 100 mg/kg and 150 mg/kg significantly increased PWT of the rats with NP implantation. Puerarin significantly reduced the expression of spinal microglia marker ionized calcium-binding adaptor molecule 1 and astrocyte marker glial fibrillary acidic protein (P<0.01). Puerarin also decreased spinal expression of pro-inflammatory cytokines tumor necrosis factor-α, interleukin-1β and interleukin-6, and increased anti-inflammatory cytokine interleukin-10 (P<0.01). CONCLUSION:Puerarin alleviate RAPLDH by inhibiting spinal glial cell activation and inflammatory response. 相似文献
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AIM: To investigate the role of Src-family kinases (SFKs) in long-term potentiation (LTP) of C-fiber-evoked potentials in rat spinal dorsal horn and the underlying mechanism. METHODS: The effect of SFKs inhibitors on spinal LTP induced by high-frequency stimulation of sciatic nerve was determined by electrophysiological method. The levels of phosphorylated SFKs (p-SFKs) in spinal dorsal horn were measured by Western blotting at different time points after LTP induction. The cell types that expressed p-SFKs following LTP induction were determined by double-labeled immunofluorescence staining. RESULTS: Electrophysiological data revealed that pretreatment with SFKs inhibitors (PP2 or SU6656) enabled high-frequency stimulation to induce long-term depression (LTD) rather than LTP in spinal dorsal horn. Western blotting analysis showed that the level of p-SFKs in ipsilateral spinal dorsal horn increased at 15 min after LTP induction. Double-labeled immunofluorescence staining demonstrated that p-SFKs were highly restricted to spinal microglia. CONCLUSION: SFKs in microglia play a critical role in the induction of LTP in spinal dorsal horn. Inhibition of SFKs and the downstream molecules may be helpful for curing neuropathic pain. 相似文献