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1.
The Green Mountain cultivar was used in field tests to determine the effects of inoculating potato plants at various times with the potato leafroll virus (PLRV) on development of internal necrosis of tuber tissue. Viruliferous apterae of the green peach aphid,Myzus persicae (Sulz.), were placed on each stem in all hills to be inoculated in each 3.0 m single-row plot. Planting and inoculation dates were varied in all field experiments and, in one, several vine-killing dates were also included. All harvested tubers were stored for approximately four months at 10°C to enhance development of internal necrosis prior to examination. Similar but smaller greenhouse studies involving both apterous and alate green peach aphids were also conducted using Green Mountain, Irish Cobbler, and Russet Burbank cultivars. All results showed that as inoculation was delayed relative to plant development, more net necrosis (NN) occurred. Conversely, when plants were inoculated early, stem-end browning (SEB) rather than NN predominated. A high percentage of naturally occurring SEB tubers (cv. Russet Burbank) were found by ELISA to contain PLRV. Plants produced by these tubers only rarely developed leafroll symptoms. These findings suggest a previously unsuspected causal relationship between SEB and PLRV. Implications of this apparent relationship on the epidemiology of potato leafroll in Maine are discussed.  相似文献   

2.
Potato cultivars Norland, Kennebec, and Russet Burbank were inoculated with a Minnesota isolate of potato virus S (PVS) in the field. The incidence of secondary infection, measured by enzyme-linked immunosorbent assay (ELISA), indicated that significantly more PVS infection occurred in Russet Burbank than Norland or Kennebec, and that Norland had significantly more infection than Kennebec (P≤ 0.01). Mature plant resistance was not observed. Rapid reinfection of PVS-free seedlots resulted from inoculum point-sources when routine cultivation practices were followed. Reinfection rates were significantly greater for seedlots grown at Grand Forks, ND, when compared with seedlots grown at Becker, MN, during both years of the study (P≤ 0.01). After two years in the field, reinfection rates for Norland (71.8%) and Russet Burbank (73.0%) did not differ significantly, but were significantly greater than the reinfection rate for Kennebec (29.5%) (P≤ 0.01). In greenhouse-grown plants, PVS moved out of rub-inoculated leaves within 24 hr, however, 13 and 20 days were required before PVS was detected with ELISA in foliage above and below the inoculated leaf, respectively. The frequency of PVS detection was significantly greater in foliage above the inoculated leaf compared to foliage below the inoculated leaf (P≤ 0.05). Translocation of PVS from inoculated leaves to daughter tubers occurred within 13 days for Russet Burbank and Norland and within 20 days for Kennebec.  相似文献   

3.
Potato varieties Russet Burbank and Ranger Russet were transformed with a cDNA version of the 23 kD coat protein cistron (CP) of the potato leafroll virus (PLRV) using anAgrobacterium-mediated procedure. Clones were assayed for presence of thenpt- II and CP genes by Southern analysis, for expression of CP mRNA by Northern analysis, and for presence of PLRV coat protein in uninoculated and aphid inoculated plants by ELISA and Western blot analysis in uninoculated plants. Two putative Russet Burbank transformants were escapes, lacking eithernptll or CP, while one putative Ranger Russet transformant possessed thenpt- II gene but not the CP gene. In Russet Burbank and Ranger Russet, some transformants had statistically lower virus titer. The lowered titer was consistent throughout assays at three times during primary infection and one assay of secondary infection. The ranking of virus titer across all tests was statistically consistent. Transformants with the lowest secondary titers had virus contents 15 and 31 % of the titers of untransformed controls for Russet Burbank and Ranger Russet, respectively. The virus titer of the two Russet Burbank escapes and the Ranger Russet with only thenpt- II gene did not differ significantly from their respective untransformed controls.  相似文献   

4.
The Agricultural Experiment Stations of Oregon, Idaho, and Washington and the U.S. Department of Agriculture jointly released Wallowa Russet in 2002. This late maturing variety is most suitable for frozen french fry use, but may find limited fresh market potential because of outstanding flavor and texture when baked. Wallowa Russet was evaluated as AO87277-6 in Oregon irrigated trials from 1993 through 1999, and in western regional trials from 1997 through 1999. Averaged over 13 locations in seven states, Wallowa Russet produced total yields of 55.6 mt/ha compared to 53.5 and 45.7 mt/ha for Russet Burbank and Russet Norkotah, respectively, and U.S. No.1 yields of 44.4 mt/ha compared to 30.7 and 36.0 mt/ha for Russet Burbank and Russet Norkotah. Specific gravity is consistently higher for Walowa Russet than for Russet Burbank and fry color is as light or lighter; sugar end development is less than that observed in Russet Burbank. Wallowa Russet is similar to Russet Burbank in susceptibility toVerticillium wilt, foliar early blight (Alternaria solani), and potato leafroll virus (PLRV). However, Wallowa Russet develops less net necrosis than Russet Burbank from PLRV infection. Wallowa Russet is more susceptible to common scab (Streptomyces scabies) than Russet Burbank. It has moderate resistance to potato virus Y (PVY). Tubers of Wallowa Russet are less susceptible to tuber infection and decay caused by potato late blight (Phytophthora infestans) than Russet Norkotah and Ranger Russet. Wallowa Russet has less hollow heart, brown center, blackspot bruise, and fewer growth cracks than Russet Burbank but is more susceptible to shatter bruise. Vitamin C is higher for Wallowa Russet (30.4 mg/100 g fresh weight) than for Russet Burbank (21.9) or Russet Norkotah (22.0), and protein content averaged 5.6% compared to 4.7% and 4.9% for Russet Burbank and Russet Norkotah, respectively.  相似文献   

5.
The spread of potato leaf roll virus (PLRV) and the resulting phloem necrosis in the tubers has been a limiting factor in the production of the Netted Gem (Russet Burbank) variety in southwestern British Columbia, Canada. Steckling sugar beets are an important overwintering host for nymphs and adults of the green peach aphid,Myzus persicae (Sulzer). By June, aphids are widely disseminated to the florets and new leaves of sugar beet and other hosts, including potato. Colonies with alates are produced on summer hosts which are not treated with insecticides. The peak of the flight of alates from the colonies on the summer hosts occurs during the first half of August. Those alates that were produced on PLRV-infected potato and which travel to other potatoes transmit the virus as soon as they feed. By the end of August, the sugar beet seed crops and the early and mid-season potato crops have been defoliated or harvested. Thus many colonies ofM. persicae are destroyed and the threat of PLRV spread diminishes. Crop and weed plants which serve as overwintering hosts become infested during late summer with alate aphids. Their offspring survive the winter in numbers which are determined by the weather and survival of host plants.  相似文献   

6.
The transmission of PVS by the cutting knife in potato cvs Norland, Kennebec and Russet Burbank was studied in the field and greenhouse. Field studies showed transmission to Norland via tuber infection was significantly less than for Kennebec and Russet Burbank (P = 0.01). However, significant cultivar differences were not found in the greenhouse (P = 0.05). Contamination of the cutting knife by cutting through sprouts resulted in significantly greater numbers of plants infected for cv Kennebec than when sprout contact was avoided (P = 0.05). Although the same trends were observed for cv Norland and Russet Burbank the differences were not significant (P = 0.05). Infectious PVS particles were readily recovered from materials commonly contacted during potato cultivation, storage and processing. Retention time of infectious PVS was usually greater when materials were held at 4°C and 100% relative humidity than at ambient greenhouse conditions. Retention times ranged from 0 hr (PVS not recovered) to 180 hr on unpainted wood. Results demonstrated the need for strict indexing and sanitation procedures during the production of PVS-free potato seed.  相似文献   

7.
Response to foliar late blight for Superior and Sebago was not affected by the two potato seed selection and multiplication methods. Kennebec and Russet Burbank potato plants derived throughin vitro tissue culture techniques had significantly more late blight damage on only 4 of 7 and 2 of 7 observation dates, respectively, than plants derived through a clonal selection system. In practical terms, the disease response differences between the two seed propagation methods were minimal. Similarly, disease response differences among the 2 and 3 years of field multiplication for Russet Burbank and Kennebec, respectively, did not demonstrate any significant disease response trends. In general,in vitro culture plots had slightly higher yields than clonal plots but only Kennebec had a significant yield response. The incidence of late blight storage rot was generally not significantly different but clonally selected Kennebec potatoes had significantly more disease than those produced throughin vitro culture.  相似文献   

8.
Hairy nightshade, Solanum sarrachoides (Sendtner) is an annual solanaceous weed prevalent in potato farmlands of the Pacific Northwest. S. sarrachoides is also a reservoir for Potato leafroll virus (PLRV) and its most important vector, the green peach aphid, Myzus persicae (Sulzer). Green peach aphids prefer S. sarrachoides than potato, Solanum tuberosum (L.), and produce more nymphs on S. sarrachoides than on potato. Increased green peach aphid preference for S. sarrachoides and greater fecundity on this plant could lead to overcrowding and movement toward neighboring potato plants. To test our hypothesis, field trials were conducted at Kimberly, Idaho during the summers of 2003 and 2004. A row of S. sarrachoides seedlings was planted in the middle of potato plots and naturally occurring green peach aphid populations were monitored weekly on potato plots with and without S. sarrachoides. More aphids were consistently found on S sarrachoides plants than on potato plants. More aphids were found on potato plants in plots with S. sarrachoides plants than without S. sarrachoides. Targeted insecticide applications on S. sarrachoides rows alone reduced the number of aphids on potato plants in those plots, suggesting that insecticide sprays prevented aphid movement from S. sarrachoides to potato. Testing of potato tuber sprouts 90 days after harvest by double antibody sandwich ELISA indicated a greater PLRV infection rate on plots with S. sarrachoides than plots without S. sarrachoides. Therefore, the presence of S. sarrachoides in potato plots will likely increase aphid populations and PLRV incidence on neighboring potato plants.  相似文献   

9.
Umatilla Russet, a moderately late maturing variety especially suitable for frozen French fry processing but also acceptable for tablestock use (boiling, and baking), was jointly released by the Agricultural Experiment Stations of Oregon, Idaho, and Washington and the U.S. Department of Agriculture in 1998. Umatilla Russet was evaluated in irrigated trials in Oregon from 1988 to 1997, and in western regional trials from 1989 to 1991. Total yields were similar to those of Russet Burbank, but Umatilla Russet produced higher marketable yields. In three years of regional trials at up to 13 locations in seven western states, Umatilla Russet averaged 40.5 Mg/ha compared with 32.4 Mg/ha for Russet. Burbank. Umatilla Russet fry color and specific gravity are consistently equal to or better than for Russet Burbank. Umatilla Russet is less susceptible toVerticillium wilt than Russet Norkotah, less susceptible to net necrosis than Russet Burbank, and resistant to PVX. It is susceptible to PLRV and expresses foliar symptoms of PVY more clearly than Russet Norkotah. Umatilla Russet is less susceptible to tuber infection and decay caused byPhytophthora infestans than Ranger Russet and Russet Norkotah. Umatilla Russet is less susceptible to hollow heart, brown center, growth cracks, and sugar ends, but more susceptible to blackspot and shatter bruise than Russet Burbank  相似文献   

10.
Increasing prevalence of potato leafroll virus (PLRV) and potato virus Y (PVY) has been reported in seed and commercial potato production, resulting in the rejection of potatoes for certification and processing. Host plant resistance to PLRV and PVY and their primary vector, green peach aphid,Myzus persicae, could limit the spread of these viruses. Host plant resistance to PLRV, PVY, and green peach aphid has been identified in non-tuber-bearingSolanum etuberosum (PI 245939) and in its backcross 2 (BC2) progeny. Resistance to green peach aphid involved a reduction in fecundity and adult aphid size. In addition, one BC2 individual was identified as possessing a genetic factor that was detrimental to nymph survival. PVY resistance was identified in all five BC2 progenies evaluated in a field screening under intense virus pressure. PLRV resistance was identified in two of the five BC2 progeny. This resistance was stable in field and cage evaluations with large populations of viruliferous aphids. Based on the segregation of virus resistances in the BC2 , PVY and PLRV resistances appear to result from the action of independent genetic mechanisms that reduce the levels of primary and secondary virus infection. Two BC2 individuals, Etb 6-21-3 and Etb 6-21-5 were identified as having multiple resistances to PLRV, PVY, and green peach aphid derived fromS. etuberosum. This germplasm could prove useful to potato breeders in the development of virus-resistant cultivars.  相似文献   

11.
The effect ofFusarium solani (Fsc),F. sambucinum (Fs),Erwinia carotovora subsp.atroseptica (Eca), andE. carotovora subsp.carotovora (Ecc) inoculated singly and in combination (FscFs, FscEca, FscEcc, FsEca, FsEcc, and EcaEcc) on wound-healed potato seed pieces of the cultivar Kennebec was studied. Potato seed pieces wound-healed for 5 days at 13 C and approximately 100% relative humidity, inoculated with Fsc, Fs, Eca, Ecc and their combinations, and incubated for 10 days at 9 C were protected from Eca, Ecc, and EcaEcc. When wound-healed potato seed pieces were inoculated with Fsc, Fs, Eca, Ecc, and their combinations and planted in soil maintained at 7, 10, 15, 20 and 25 C, wound-healing protected seed pieces from all pathogens and their combinations in seed pieces held for 10 days at 10 and 15 C. When seed pieces of cultivars Atlantic, Kennebec, Norchip, Russet Burbank and Superior were inoculated with Fsc, Fs, Eca, Ecc, and their combinations, different levels of protection were observed: Russet Burbank had the highest level of protection, Atlantic and Kennebec were intermediate, and Norchip and Superior were slightly protected. Dusting mancozeb on wound-healed seed pieces before inoculation with Fsc, Fs, Eca, Ecc, and their combinations increased protection against all pathogens and their combinations by 39% or more compared to the nonchemical control.  相似文献   

12.
‘Alturas’, a late-maturing, high-yielding, russet potato cultivar with high tuber specific gravity, was released in 2002 by the USDA-ARS and the Agricultural Experiment Stations of Idaho, Oregon, and Washington. Originally selected for dehydration processing, its cold-sweetening resistance also makes it suitable for processing out of storage into french fries and other frozen potato products. Culinary quality is high, with larger tubers suitable for fresh market if heavily russeted skin is not essential. Alturas consistently produced greater total and U.S. No. 1 yields than ‘Russet Burbank’ and ‘Ranger Russet’ in southern Idaho trials. Across other western trial sites, total yields of Alturas have on average been 29% and 14% greater than Russet Burbank and Ranger Russet, respectively. Alturas is resistant to Verticillium wilt (Verticillium dahliae) and early blight (Alternaria solani). Compared with Russet Burbank and Ranger Russet, Alturas is less susceptible to corky ringspot and foliar and tuber infection by late blight (Phytophthora infestans). It also is less susceptible to tuber net necrosis and Fusarium dry rot than Russet Burbank, and is more resistant to common scab (Streptomyces scabies) than Ranger Russet. However, Alturas is more susceptible to infection by PVY and PVX than Ranger Russet. Total nitrogen application recommendations for Alturas are approximately 40% less than those for Russet Burbank. Alturas requires 15% to 20% more water during the growing season than does Russet Bur-bank.  相似文献   

13.
GemStar Russet, derived from the cross Gem Russet x A8341-5, was released in 2004 by the USDA/ARS and the agricultural experiment stations of Idaho, Oregon, and Washington. The foliage of GemStar Russet is dark yellowish-green, spreading, with large leaves and abundant white flowers. The tubers are brown, medium to heavily russeted, oblong, with a slightly prominent eyebrow, white flesh, and indistinct pith. GemStar Russet was compared with Russet Burbank and Russet Norkotah in trials across the Pacific Northwest for yield, quality, and disease response. Except for locations in southern Idaho, in general, GemStar Russet produced slightly lower total yield than Russet Burbank, but much higher U.S. No. 1 yield. When compared with Russet Norkotah, GemStar Russet produced similar total and U.S. No. 1 yields in early harvest trials but higher total and U.S. No. 1 yields in late-harvest trials. When observed for defect problems, GemStar Russet exhibited resistance to second growth, growth cracks, and stemend discoloration, moderate resistance to blackspot and shatter bruising, but a high level of susceptibility to hollow heart. In product quality tests, GemStar Russet was rated superior to Russet Burbank for french fry quality and comparable for baked potato quality. GemStar Russet was found to be immune to PVX, resistant to common scab and powdery scab, moderately resistant to Verticillium wilt, tuber net necrosis caused by PLRV, and corky ringspot. It demonstrated susceptibility to late blight, PLRV, dry rot, soft rot, and ringrot and extreme susceptibility to PVYo. Biochemical analysis of GemStar Russet tubers showed them to be higher in protein and much higher in vitamin C than those of Russet Burbank or Russet Norkotah. Three-year average for tuber glycoalkaloid concentration was 1.5 mg 100 g?1.  相似文献   

14.
AC Brador is a late maturing yellow-fleshed cultivar. The vine maturity is slightly later than Russet Burbank. The oblong tubers have a specific gravity which is slightly higher than Kennebec. The boil and bake quality of AC Brador is similar to Kennebec. The mean French fry quality of AC Brador is below that of Russet Burbank but still in the acceptable range. This cultivar has extremely high field resistance to late blight resistance to verticillium wilt and early blight, a relatively high resistance to PLRV, and a moderate resistance to PVYand rhizoctonia.  相似文献   

15.
Potato virus Y (PVY) infection is one of the greatest challenges to seed potato production in the United States. To determine how cultivar and seed type affect the development of systemic PVY infection, Russet Burbank and Russet Norkotah Colorado 3 cultivars were grown from two types of pre-nuclear seed (i.e., plantlets and minitubers) and Generation 3 (G3) tubers and challenged with PVY strain Wilga (PVYN-Wi). Systemic PVY infection was measured by assaying spread of virus from the inoculation site to upper non-inoculated leaves. The Burbank cultivar had a lower incidence of systemic PVY infection compared to the incidence of systemic PVY that developed in the Colorado 3 cultivar. Furthermore, Burbank plants grown from G3 tubers had a lower incidence of systemic PVY infection, as compared to Burbank plants grown from plantlets. Together our results indicate that both cultivar and seed type affect the development of systemic PVYN-Wi infections post-inoculation.  相似文献   

16.
The effect of daminozide [Butanedioic acid mono (2,2-dimethyl hydrazide)] on growth, field establishment and productivity of micropropagated Kennebec and Russet Burbank potato was studied. Addition of daminozide to the medium significantly reduced stem length ofin vitro propagated plantlets. Optimal dose was 0.6 mg active ingredient per plant. This dose did not significantly alter the number of nodes and leaf area. Three transplanting methods were examined: direct from tissue culture to the field without protection; the same but with a plastic covering for protection; and after 7 days acclimation in a greenhouse but without protection in the field. Stand establishment ranged from 83.3 to 100%. The second transplanting method had the highest percentage establishment for both cultivars. The application of daminozide improved survival for all transplants of Kennebec and the directly planted plantlets of Russet Burbank. Independent of the daminozide treatment, plants of both cultivars hardened in the greenhouse prior to transplanting produced the highest number and yield of tubers. For Russet Burbank, daminozide caused a delay in tuberization but did not affect tuber bulking and haulm growth.  相似文献   

17.
Russet Legend, a moderately late maturing variety especially suitable for frozen French fry processing but also acceptable for tablestock use (boiling, and baking), was jointly released by the Agricultural Experiment Stations of Oregon, Colorado, Idaho, and Washington, and the U.S. Department of Agriculture in 1998. Russet Legend was evaluated in irrigated trials in Oregon from 1988 to 1997, and in western regional trials from 1990 to 1992. Total yields were similar to those of Russet Burbank, but Russet Legend produced higher marketable yields. In three years of regional trials at up to 13 locations in seven western states, Russet Legend averaged 47.3 Mg/ha marketable yield compared with 36.5 Mg/ha for Russet Burbank and 33.6 Mg/ha for Russet Norkotah. Russet Legend has consistently achieved lighter fry color and higher specific gravity than Russet Burbank. Russet Legend is less susceptible toVerticillium wilt than Russet Burbank or Russet Norkotah. It is susceptible to PLRV and net necrosis, and expresses PVY foliar symptoms clearly. Russet Legend is less susceptible to tuber infection and decay caused byPhytophthora infestans than Ranger Russet and Russet Norkotah. Russet Legend is less susceptible to hollow heart, brown center, growth cracks, and sugar ends than Russet Burbank  相似文献   

18.
Since 1964, replicated rotation plots in Presque Isle, Maine have continually been planted and evaluated to determine the effect of various rotation crops on tuber appearance, quality, and yield. In 1974 and 1975, the saprophytic activity ofRhizoctonia solani in the soils of each rotation combination was determined, and the tubers harvested were scored forRhizoctonia damage. Soils with a two-year rotation of oat-potato had the lowest amount of saprophytic activity byRhizoctonia and provided the lowest incidence of disease on the stems, roots, and tubers of the cultivars Kennebec and Katahdin. With the cultivar Russet Burbank, the three-year rotations generally provided the lowest incidence of disease; however, this cultivar is more susceptible to the pathogen. The plots of potatoes that were not rotated had the highest pathogen activity and the greatest disease incidence.  相似文献   

19.
Green peach aphid Myzus persicae (Sulzer) is a serious pest of potato and causes severe yield loss to potato production worldwide. The volatiles emitted from potato plants influence host selection by M. persicae. A laboratory experiment was conducted to study how these volatile emissions vary among four potato varieties (IdaRose, Desiree, Chipeta and Russet Burbank) having different genetic backgrounds, and to compare the aphid responses to the volatiles emitted by these varieties. Results indicated that headspace volatiles detected, and aphid responses, differed significantly among the varieties. Myzus persicae arrestment was greater in response to Desiree and Chipeta than on Russet Burbank and arrestment on IdaRose was intermediate and did not differ significantly from any of the other varieties, but the arrestment was not related to the total concentration of volatiles released by these varieties. This indicates that the blend of volatiles was important for eliciting arrestment. Eigenvector weightings of principal component 1 were predominantly sesquiterpenes (especially caryophyllene, sesquiphellandrene, α-farnesene and longifolene), whereas those for principal component 2 were predominantly aldehydes and green leaf volatiles (n-hexana1, (Z)-3-hexenol, (E)-2-hexenal and nonanal. The pedigrees of the varieties are not associated with the volatile blends or aphid responses.  相似文献   

20.
Russet Burbank and Kennebec potatoes were evaluated over 5 years at 3 planting dates, 3 plant spacings, and 3 nitrogen rates. Delayed planting significantly reduced Russet Burbank yield, but did not effect Kennebec yield. Both varieties exhibited reduced specific gravity of tubers with delayed planting. Spacings between 38 and 56 cm for Russet Burbank and 20 and 38 cm for Kennebec had no significant effect on yields of “A” size tubers, but wider spacing reduced specific gravity of tubers. No yield advantage occurred with nitrogen application greater than 134 kg N/ha. Increased nitrogen application reduced specific gravity of tubers. Significant differences in the N, P, and K levels of leaves, petioles, and tubers were observed due to some treatments.  相似文献   

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