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从某养殖场的病死鸡中分离获得1株巴氏杆菌,血清型鉴定为A型。攻毒试验中,试验鸡表现出巴氏杆菌病的典型临床症状;采集病死试验鸡的心脏、肝脏病料,以同样方法分离到巴氏杆菌。以柯氏法则及药敏试验结果诊断该鸡场发生巴氏杆菌病且菌株的致病力较强。本研究为该病的实验室诊断及防控提供了方法。 相似文献
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鸡巴氏杆菌的分离鉴定及体外抑制实验 总被引：1，自引：0，他引：1
为了诊断一起鸡暴发性死亡病例,对死亡鸡肝脏进行细菌分离、生理生化实验、药敏实验、动物致病性实验。结果:分离菌生理生化特性与巴氏杆菌标准株基本一致;分离菌对青霉素类抗生素完全耐药,对卡那霉素、红霉素、氧氟沙星等中度敏感;分离菌肌注健康试验鸡0.5m L,鸡12h内死亡,口服0.5m L,鸡24h内死亡;鸡唾液乳酸杆菌对分离菌有较强的抑制作用。结论:分离菌为多杀性巴氏杆菌强毒株,该病例是由多杀性巴氏杆菌引起的鸡巴氏杆菌病,有效药物为:卡那霉素、红霉素、氧氟沙星等,鸡唾液乳酸杆菌可减少或部分取代抗生素对该病的使用。 相似文献
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本试验共收集了死亡畜、禽、胚病料537份,分离出致病菌220株。其中鸡97羽,分离出多杀性巴氏杆菌64株,葡萄球菌1株;火鸡76羽,分离出多杀性巴氏杆菌17株,葡萄球菌1株;猪51头,分离出多杀性巴氏杆菌8株,猪丹毒杆菌10株,大肠株菌2株;羊13头,分离出葡萄球菌2株,大肠杆菌3株;兔9只,分离出多杀性巴氏杆菌1株;火鸡死胚255枚,分离出葡萄球菌42株,沙门氏杆菌33株,绿脓杆菌15株,大肠杆菌4株,链球菌2株;死亡鸡胚25枚,分离出绿脓杆菌8株,葡萄球菌4株,大肠杆菌3株。上述结果表明:致死家禽的病原菌主要有多杀性巴氏杆菌和葡萄球菌。家畜除此而外还有猪丹毒杆菌和大肠杆菌。致死胚胎的细菌主要有葡萄球菌、沙门氏菌、绿脓杆菌和大肠杆菌。本试验为减少畜禽的死亡和疫病的防治提供了依据,同时保存了地方菌株,为今后的科研和生产提供了保贵资料。 相似文献
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兔巴氏杆菌病(Rabbit Pasteurellosis)是由多杀性巴氏杆菌引起的,严重危害养兔生产的重点传染病,也是一种人畜共患的传染病。本试验从保定市某县獭兔养殖场病死兔肝脏和鼻咽部分分离到的一株细菌,通过对病死兔的病理解剖、细菌的分离、筛选、纯化、鉴定。结果显示该株细菌为多杀性巴氏杆菌。 相似文献
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对1例疑似鸭肝炎病毒和多杀性巴氏杆菌混合感染的10日龄肉鸭采用常规的病毒、细菌鉴定方法和RT-PCR、PCR方法分别进行病毒、细菌的分离与鉴定。病毒鉴定为新型鸭肝炎病毒,细菌鉴定为荚膜血清A型多杀性巴氏杆菌多杀亚种。细菌对SPF鸡的毒力试验结果显示,分离的巴氏杆菌与强毒标准株C48-1毒力相近,为强毒株。细菌对10日龄肉鸭的致病性回归试验结果表明,一定数量的该株巴氏杆菌可导致10日龄雏鸭的感染死亡。结果表明,该批肉鸭为新型鸭肝炎病毒和A型多杀性巴氏杆菌混合感染。这是国内首例从感染鸭肝炎病毒10日龄雏鸭肝脏中分离到多杀性巴氏杆菌。 相似文献
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大丰市某禽场4000只49周龄笼养产蛋鸡，发生以产蛋急剧下降，死亡数增加，卵巢输卵管病变以及卵黄性腹膜炎为主的病情。发病2周，产蛋率下降了28%，死亡率为12%。从患病死亡鸡肝脏分离到一株细菌，经细菌形态、培养特性、生化鉴定、动物接种等试验，诊断为鸡巴氏杆菌病。 相似文献
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泛达宁－１０对鸡大肠杆菌病和巴氏杆菌病药效试验王树槐孙玉梅中国兽药监察所北京１０００８１泛达宁－１０为台湾派斯德公司生产氟喹诺酮类抗菌药物制剂。该制剂通过破坏细菌ＤＮＡ合成而达到抑杀细菌的作用。本试验对泛达宁－１０进行了大肠杆菌Ｃ８３８８２、巴氏杆菌... 相似文献
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Investigation was done to determine the presence of Pasteurella multocida and related species in free ranging chickens and ducks, dogs, cats and pigs in three climatic zones (cool, warm and hot) of rural Morogoro, Tanzania. A total of 153 isolates of P. multocida ssp. multocida and related species were obtained by direct culture on blood agar, selective medium and mouse inoculation. P. multocida ssp. multocida was isolated from 0.7% of chickens and 7% of ducks. In dogs and cats, P. multocida ssp. multocida was isolated from 1 and 68%, respectively. One isolate of Pasteurella gallinarum was isolated from a duck. Other species obtained were; P. multocida ssp. septica, Pasteurella stomatis and taxon 16 from dogs and cats, while Pasteurella dagmatis and Pasteurella canis were found in dogs only. Prevalence of P. multocida ssp. multocida was significantly higher (P<0.01) in ducks of the warm zone (22%) than in ducks of other zones (0%). No significant difference was observed between the prevalence of P. multocida ssp. multocida in chickens of the warm zone (2%) and chickens of the cool and hot zones (0%). Extended phenotypic characterization revealed phenotypic similarities between two isolates from chickens and the duck strains. Mouse inoculation appeared to be more sensitive in detecting P. multocida ssp. multocida than blood agar and selective medium. Direct culture on blood agar recovered most of the isolates from dogs. This study has demonstrated for the first time the presence of P. multocida and related species in the village free ranging chickens, ducks, dogs and cats in Tanzania. Other non-classified Pasteurella spp. were also observed in the study, but further characterization is required before the final classification can be made. This paper reports for the first time the isolation of unclassified Pasteurella from dogs and cats in Africa. The results implies that fowl cholera might be occurring in free ranging poultry, and dogs and cats kept in contact might serve as sources of P. multocida to chickens and ducks. Subsequent applications of molecular techniques to analyse the epidemiological relatedness of clones isolated from different host species is indicated. 相似文献
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Sthitmatee N Kataoka Y Sawada T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(11):1445-1451
A mutant strain, PBA322, was constructed by electroporation of a phagemid containing the coding region of antisense RNA of the ompH gene, encoding 39 kDa capsular protein or OmpH, into the parental strain P-1059 (serovar A:3) of Pasteurella multocida, and the pathogenicity was determined in mice and chickens. Grayish colonies of the mutant, indicating loss of capsule synthesis, were observed under a stereomicroscope using obliquely transmitted light, while iridescent colonies were observed for the parental strain. Moreover, strain PBA322 showed a low amount of OmpH compared with the parental strain on SDS-PAGE. Additionally, the capsule of strain PBA322 was thinner than that of the parental strain according to electron microscopy, correlating to the attenuation against chickens. In conclusion, strain PBA322, the mutant of P. multocida strain P-1059, was completely attenuated for chickens. 相似文献
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从六安地区某养鸡场禽霍乱病鸡上分离到1株禽多杀性巴氏杆菌,采用纸片扩散法和试管稀释法分别测定了青霉素钾、土霉素、硫酸卡那霉素、硫酸庆大霉素、硫酸链霉素、复方新诺明、甲砜霉素、氟哌酸、环丙沙星和恩诺沙星等10种药物对分离菌的体外抗菌活性。结果表明分离到的禽多杀性巴氏杆菌对青霉素钾和土霉素耐药;对硫酸卡那霉素、硫酸庆大霉素、硫酸链霉素、甲砜霉素、氟哌酸敏感;对复方新诺明、环丙沙星和恩诺沙星极敏感。两种测定方法得到的结果一致。因此复方新诺敏、环丙沙星和恩诺沙星可作为目前该发病场防治禽霍乱的首选药物。 相似文献
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An indirect enzyme-linked immunosorbent assay (ELISA) was developed to measure humoral antibody responses of chickens against Pasteurella multocida. A standard indirect hemagglutination (IHA) test was used to compare serologic results with those of ELISA. The ELISA was also used following challenge with P. multocida to compare the efficacy of three commercial fowl cholera vaccination regimens. Although antibody titers measured by ELISA and IHA were highly correlated, ELISA was at least twice as sensitive as IHA. Antibody measured by ELISA and IHA also correlated significantly with protection against P. multocida challenge. No mortality occurred in any of the three vaccinated challenged groups. However, control unvaccinated chickens experimentally infected with P. multocida developed signs of acute pasteurellosis and died by the 10th day post-challenge. Impression smears made of hepatic tissue from all chickens were stained (Wright's stain), and typical bipolar rods characteristic of Pasteurella were identified in smears from unvaccinated challenged controls only. 相似文献
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对江西省某养鸭场送检的病死鸭进行病理剖检、细菌分离,经生化鉴定确定病原为多杀性巴氏杆菌。该分离菌株对头孢氨苄、头孢曲松、头孢唑啉、甲氧嘧啶、复方新诺明和氯霉素高度敏感;对大观霉素、卡那霉素等中度敏感;对头孢噻肟、四环素、林可霉素和诺氟沙星低度敏感;对阿莫西林、青霉素、氨苄西林和头孢吡肟不敏感。将分离菌株人工感染鸡、鸭、兔和小白鼠,感染后24 h内死亡率为100%,死亡动物均分离到与原分离菌株形态特征、培养特性一致的细菌,结果表明该分离菌株为多杀性巴氏杆菌强毒株。 相似文献
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The probability of survival of chickens following a challenge dose of the X-73 strain of Pasteurella multocida was calculated based on enzyme-linked immunosorbent assay (ELISA) titer. Chickens were vaccinated subcutaneously with the Clemson University strain of P. multocida. On days 3, 5, 7, 10, 12, 14, 21, and 28 post-vaccination, 10 vaccinated chickens and 5 unvaccinated controls were selected at random, bled, and then challenged with 2000 colony-forming units of X-73. The ELISA titers to P. multocida vaccination and responses to challenge were recorded. A logistic procedure predicted probability of survival related to ELISA titer. The ELISA titer and survival were highly correlated. A flock profile for each day of challenge was developed based on a probability of survival (PS) at the following levels: PS less than 25%, 25% less than or equal to PS less than 50%, 50% less than or equal to PS less than 75%, and PS greater than or equal to 75%. The antibody response of the chickens through 28 days post-vaccination demonstrated a classic response to vaccination. 相似文献
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Overexpression and immunogenicity of the Oma87 outer membrane protein of Pasteurella multocida 总被引：3，自引：0，他引：3
The outer membrane protein of Oma87 from Pasteurella multocida A:1 has significant similarity to the D15 protective antigen of Haemophilus influenzae (Ruffolo and Adler, 1996). Four fragments of Oma87 from a P. multocida serotype D strain were cloned into a pGEX expression vector and transformed into E. coli JM105. Western blot analysis revealed that convalescent chicken sera reacted with only GST-F1 fusion protein which contained amino acids 18 through to 130 of Oma87 fused to the GST protein. Vaccination with the GST-F1 protein failed to protect chickens against challenge with a virulent P. multocida serotype A. 相似文献
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Sarközy G Semjén G Laczay P Horváth E 《Journal of veterinary medicine. B, Infectious diseases and veterinary public health》2002,49(3):130-134
Experimental fowl cholera was induced in 60 healthy 10-week-old broiler chickens and 8-week-old turkeys by intramuscular inoculation with approximately 80 colony-forming units (cfu) of Pasteurella multocida X-73 strain and with approximately 70 cfu of P. multocida P-1059 strain, respectively. This method of infection proved to be useful for evaluating the efficacy of anti-microbial medication, by measuring mortality, weight gain, pathological responses and frequency of re-isolation of P. multocida. The efficacies of two different dosing methods, continuous and pulse dosing, were compared. Using the continuous-dosing method, norfloxacin was administered to drinking water at 100 mg/l for 5 days in chickens. Efficacies were slightly improved compared with pulse dosing at 15 mg/kg bodyweight for the same length of time. The opposite was observed in turkeys, to the degree of control of mortality and maintenance of weight gain. 相似文献
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Pasteurella multocida belonging to somatic serotype 1 and capsular type A has been known to cause avian cholera in domestic poultry. Pasteurella multocida serotype 1 has also been isolated from raptorial birds. However, the capsular type for these raptorial isolates remains unknown. Moreover, the virulence of these raptorial isolates for domestic poultry has not been determined. The objectives of this study were to determine the capsular type of raptorial P. multocida serotype 1 isolates and to determine if these isolates were virulent for domestic chickens. Study chickens were inoculated with one of three P. multocida isolates. Isolate WESO-1 was obtained from a western screech owl (Otus kennicottii) and isolates RTHA-2 and RTHA-4 were isolated from two red-tailed hawks (Buteo jamaicensis). These isolates were given by either the oral, intravenous, or intraocular route. Control birds were given brain-heart infusion broth. The capsular serotypes of three isolates were also determined. The RTHA-2 and RTHA-4 isolates belonged to P. multocida capsular type A. The WESO-1 isolate belonged to capsular type F. Results also demonstrated that, for the isolates examined, the intraocular route did not cause mortality in chickens. There was mortality in all groups for the intravenous route. However, various mortality patterns were observed when P. multocida was given orally for the three different isolates. The RTHA-4 isolate (serotype 1:A) was the most virulent for domestic chickens. The WESO-1 isolate (serotype 1:F) was the least virulent for chickens among the raptorial isolates examined. 相似文献
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Of 13 field isolates of Pasteurella from chickens and ducks in Indonesia, 10 were confirmed as P. multocida subspecies multocida, one as P. multocida subspecies gallicida and one as P. multocida subspecies septica. Nine were capsular Type A four were Serotype 1, one was Serotype 4, one was Serotype 11, one was Serotypes 4,12, and the remaining six were untypable. Five isolates were pathogenic for mice and two were pathogenic for chickens. Both a trivalent vaccine which included local field isolates and an imported commercial vaccine, were efficacious in layer chickens against challenge with virulent reference and local field strains. Though not statistically significant, the protection provided by the trivalent vaccine against virulent field isolate challenge was slightly better and could provide an improvement over the currently used imported vaccine although further field trials are required. A bacterin vaccine produced from a Serotype 1 field isolate grown in the allantoic sac of embryonated chicken eggs provided chickens with good cross protection against heterologous serotype challenge. 相似文献