共查询到20条相似文献，搜索用时 15 毫秒
1.
AIM：To investigate whether erythropoietin (EPO) attenuates cisplatin (CP)-induced nephrotoxicity by regulating unfolded protein response (UPR). METHODS：Healthy male Sprague-Dawley (SD) rats were randomly divided into control group (n=12), CP group (n=12) and CP combined with recombinant human erythropoietin (CP+rHuEPO) group (n=12). All animals were sacrificed 96 h after injection of normal saline or CP. Blood samples and kidney tissues were collected to evaluate blood urea nitrogen (BUN), serum creatinine (SCr) and the morphological alteration of the kidneys. The apoptosis of the renal tubular epithelium cells was detected by TUNEL. The protein levels of EPO receptor (EPOR) and glucose-regulated protein 78 (GRP78) were measured by the methods of Western blotting, immunohistochemistry and immunofluorescent staining. RESULTS：Compared with control group, significant increases in the levels of SCr and BUN were observed in CP group and CP+rHuEPO group, whereas SCr was significantly lower in the rats treated with rHuEPO after CP injection than that in the CP-injected rats. The histological structure of the kidneys observed by PAS staining showed marked structural damage in CP group. No or very little structural damage was detected in control group and CP+rHuEPO group. The observations of morphological evidence showed that CP caused an increase in TUNEL-positive cells and the apoptotic cell death induced by CP was significantly abrogated by rHuEPO at 96 h. The over-expression of CP-induced EPOR and GRP78 was suppressed by rHuEPO. CONCLUSION：CP activates UPR in renal tubular epithelial cells. EPO has a protective effect on the kidneys with CP-induced nephrotoxicity, which may be related to the regulation of UPR-induced apoptosis. 相似文献
2.
AIM:To investigate the effect of dexmedetomidine (DEX) on renal injury induced by lung ischemia/reperfusion (I/R) in mice and its relationship with endoplasmic reticulum stress response.METHODS:Healthy SPF male C57BL/6J mice,weighing 20~24 g,aged 8~10 weeks,were randomly divided into 5 groups (n=10 each):sham operation group (sham group),I/R group,atipamezole (Atip) group,DEX group,and DEX+Atip group.In vivo lung I/R model was established by occlusion of the left pulmonary artery for 30 min followed by 180 min of reperfusion in the mice.The Atip (250 μg/kg),DEX (20 μg/kg) and DEX+Atip were intraperitoneally infused into the mice before left pulmonary hilus was blocked in Atip group,DEX group and DEX+Atip group,and other operations were the same as I/R group.After experiment,the mice were killed,and the renal tissues were harvested to observe the morphological changes.The enzymatic activity of caspase-3,serum creatinine and blood urea nitrogen,and cell apoptotic index of the renal cells were also analyzed.The expression of c-Jun N-terminal kinase (JNK),caspase-12,CCAAT/enhancer-binding protein homdogous protein (CHOP) and glucose-regulated protein 78(GRP78) at mRNA and protein levels in the renal tissues was determined by RT-PCR and Western blot.RESULTS:Compared with sham group,the enzymatic activity of caspase-3,serum creatinine and blood urea nitrogen,renal cell apoptotic index,and the mRNA and protein levels of JNK,caspase-12,CHOP and GRP78 in I/R group were significantly increased (P<0.01),and the renal tissues had obvious damage under light microscope.Compared with I/R group,Atip group and DEX+Atip group,the enzymatic activity of caspase-3,serum creatinine and blood urea nitrogen,renal cell apoptotic index,and the mRNA and protein levels of JNK,caspase-12 and CHOP in DEX group were significantly decreased,and the expression level of GRP78 significantly increased (P<0.01).Furthermore,the renal tissue damage was obvious reduced.CONCLUSION:DEX effectively relieves the renal injury induced by lung I/R in mice,which may be associated with exciting α2-adrenergic receptor and inhibiting endoplasmic reticulum stress response. 相似文献
3.
AIM: To investigate the role of endoplasmic reticulum stress in renal injury caused by hyperlipidemia and the influence effect of simvastatin. METHODS: Thirty male Wistar rats were randomly divided into three groups: rats in control group (n=10) were fed with normal diet; rats in high fat group (n=10) were fed with high fat diet; animals in simvastatin+high fat group (n=10) were fed with high fat diet and were received simvastatin 10 mg·kg-1·d-1 by gastric irrigation. After 18 weeks, the quantitative urine protein in 24 h, the serum cholesterol and triglycerides levels were tested. The pathological changes of renal tissue were observed under optic microscope. The expressions of GRP78 and p-JNK in renal tissues were examined by immunohistochemistry. The apoptotic cells in the kidney were detected by TUNEL staining. The mRNA expressions of GRP78 and CHOP were examined by RT-PCR. RESULTS: The quantitative urine protein in 24 h, the serum lipid, the expressions of GRP78 and p-JNK proteins, the mRNA expressions of GRP78 and CHOP as well as the apoptotic cells in renal tissues were increased in high fat group (P<0.01).The quantitative urine protein in 24 h, the serum lipid, the expression of GRP78 and p-JNK proteins, the mRNA expressions of GRP78 and CHOP as well as the apoptotic cells in renal tissues were remarkably reduced in simvastatin+high fat group than those in high fat group (P<0.05). CONCLUSION: The endoplasmic reticulum stress is engaged in the renal injury caused by hyperlipidemia. The simvastatin play a role in renal protection by inhibiting the endoplasmic reticulum stress in the kidney. 相似文献
4.
AIM:To study the effects of taurine at different doses on renal oxidative stress and inflammation induced by paraquat in rats.METHODS:Male SD rats (n=48) were randomly divided into 4 groups:negative control group,paraquat group,paraquat+low-dose taurine group,and paraquat+high-dose taurine group.The serum levels of creatinine and urea nitrogen were detected by a biochemical analyzer.The levels of malondialdehyde (MDA) and superoxide dismutase (SOD) were measured by colorimetry.The plasma concentrations of IL-6 and intercellular adhesion molecule (ICAM)-1 were detected by ELISA.Renal reactive oxygen species (ROS) was checked by fluorescence probe dihydroethidium (DHE).The protein levels of renal p-P38 MAPK,p-ERK1/2 and p-JNK were determined by Western blot.The mRNA expression of TNF-α,IL-6 and TGF-β1 was detected by real-time PCR.RESULTS:Serum creatinine and urea nitrogen increased after paraquat poisoning,and decreased after feeding with taurine in poisoned rats,with better result in high-dose taurine group.Taurine reduced the oxidative stress and inflammation in the renal tissue,and also reduced the protein levels of p-JNK,p-ERK1/2 and p-P38 in the kidney of paraquat-poisoned rats.CONCLUSION:Taurine attenuates renal injury induced by paraquat poisoning in rats.The mechanism may be related to reducing renal MAPK activity,oxidative stress and inflammatory response. 相似文献
5.
AIM: To explore the effect of acteoside on behavioral changes and endoplasmic reticulum stress (ERS) in prefrontal cortex of depressive rats. METHODS: Sprague-Dawley (SD) rats (n=108) were randomly divided into 6 groups:control group, model group, fluoxetine (20 mg/kg) group, low-dose (30 mg/kg) acteoside group, medium-dose (60 mg/kg) acteoside group and high-dose (120 mg/kg) acteoside group, with 18 rats in each group. The depressive-like rat model was established by chronic unpredictable mild stress (CUMS) combined with solitary way for 28 d. The rats in fluoxetine group and acteoside groups were treated with fluoxetine (20 mg/kg) or acteoside (30 mg/kg, 60 mg/kg and 120 mg/kg) once daily by intragastric administration for 3 weeks. The rats in control group and model group were both given equal volume of saline by intragastric administration for 3 weeks. The behavioral changes were detected by the open-field test and sugar preference experiment. The protein expression of glucose-regulated protein 78 (GRP78) and C/EBP homologous protein (CHOP) was assessed by immunofluorescence and Western blot. The caspase-3 activity was measured by spectrophotometer. RESULTS: Compared with control group, the total distance, time spent in the center and sugar intake were all decreased, the expression of GRP78 and CHOP was increased, and the activity of caspase-3 was increased in model group, fluoxetine group and acteoside groups (P<0.05). Compared with model group, the total distance, time spent in the center and sugar intake were increased, the expression of GRP78 and CHOP was reduced, and the activity of caspase-3 was decreased (P<0.05) in fluoxetine group and acteoside groups. CONCLUSION: Acteoside improves depressive-like behaviors in depressive rats, which may be related to the inhibition of ERS and neuronal apoptosis in prefrontal cortex. 相似文献
6.
AIM:To study the effects of angiotensin Ⅱ type 1 receptor autoantibody (AT1-AA) on the apoptosis of renal cell and the expression of c-Jun N-terminal kinase (JNK) in diabetic nephropathy (DN) rats. METHODS:High-sucrose and high-fat diet and intraperitoneal injection of streptozotocin were utilized to induce DN rat model. We employed enzyme-linked immunosorbent assay (ELISA) for serum AT1-AA and TUNEL staining for renal cell apoptosis. Furthermore, Western blotting was performed to measure the levels of endoplasmic reticulum stress (ERS) chaperone glucose-regulated protein 78 (GRP78) and ERS-associated apoptosis protein p-JNK. RESULTS:The renal cell apoptotic rate in DN group was significantly increased compared with NC group, and the apoptotic renal cells in AT1-AA positive DN rats were much greater than those in AT1-AA negative DN rats (P<0.05). The protein levels of GRP78 and p-JNK were significantly increased compared with NC group. GRP78 and p-JNK protein levels also significantly increased in AT1-AA positive DN rats compared with AT1-AA negative DN rats. CONCLUSION: AT1-AA activates ERS response and induces renal cell apoptosis via the JNK apoptotic pathway in the renal tissues of DN rats. 相似文献
7.
AIM: To investigate the effects of tetramethylpyrazine combined with aminoguanidine on the renal functions of neonatal-0 streptozotocin-induced (n0-STZ) rats. METHODS: Neonatal Wistar rats were intraperitoneally injected with a single dose of streptozotocin (STZ) to establish the n0-STZ rat model. The n0-STZ rats were divided into 4 groups: normal control group, insulin resistance group, metformin treatment group and tetramethylpyrazine+aminoguanidine treatment group. Fasting plasm glucose, fasting insulin, insulin resistance index, blood urea nitrogen, serum creatinine, urine albumin and glomerular filtration rate were measured at the 32nd week. The mRNA content of inducible nitric oxide synthase (iNOS) in peripheral blood leukocytes was detected by the technique of in situ hybridization. Nitric oxide (NO) concentration, iNOS activity, the protein expression of iNOS and 3-nitrotyrosine(3-NT) were also assessed in the renal tissues. RESULTS: At the 8th week after the administration of STZ, 82.5% of Wistar rats showed that the fasting plasm glucose level was ≥7.0 mmol/L and the renal functions were seriously damaged. Although both metformin and the combined treatment reduced fasting plasm glucose, fasting insulin and insulin resistance index, the combined treatment was superior in improving the insulin resistance. The damaged renal functions were improved by the combined treatment as reducing blood urea nitrogen and creatinine, increasing glomerular filtration rate were observed. Furthermore, the combined treatment reduced NO concentration, decreased iNOS activity and diminished mRNA content of iNOS, resulting in depressing the generation of 3-NT and iNOS, which surpassed the treatment of metformin. CONCLUSION: Tetramethylpyrazine combined with aminoguanidine improves the renal functions of n0-STZ rats by depressing nitrative stress and enhancing the effect of metformin. 相似文献
8.
AIM: To investigate the potential mechanisms of renoprotective effect of grape seed proanthocyanidin (GSP) on diabetic nephropathy.METHODS: Male Wistar rats were injected with 1% streptozotocin (STZ) intravenously to induce diabetes mellitus (DM). The diabetic rats were randomly divided into 2 groups: diabetes group (DM group) and GSP treatment group (GSP group, GSP 250 mg·kg-1·d-1). The normal Wistar rats served as control (C group). Body weight (BW), systolic pressure, kidney weight/body weight (KW/BW), fasting plasma glucose (FPG), blood urea nitrogen (BUN), serum creatinine (SCr), glycosylated hemoglobin (HbA1c) and 24 h urine protein were determined 24 weeks after STZ intervention. The pathological changes of the renal tissues were observed. The protein levels of glutathione S-transferase mu (GSTM) and nuclear factor-erythroid 2-related factor 2 (Nrf2) in the renal tissues were determined by Western blotting and immunohistochemistry. RESULTS: Compared with C group, BW in diabetic rats decreased (P<0.01). The levels of systolic pressure, FPG, HbA1c, KW/BW, 24 h urine protein, BUN and SCr in DM group were higher than those in C group (P<0.01). After treated with GSP, the levels of systolic pressure, KW/BW, 24 h urine protein, BUN and SCr in DM rats were lower than those in DM rats without treatment (P<0.01 or P<0.05). The pathological changes were ameliorated in GSP group. The expression of GSTM and Nrf2 was up-regulated in the kidneys of diabetic rats and down-regulated to the normal levels after GSP treatment. CONCLUSION: The renoprotective effect of GSP is associated with the down-regulation of GSTM through modulating the expression of Nrf2. 相似文献
9.
Prevention and treatment of Hua Yu Dao Zhi decoction in renal fibrosis induced by gentamicin in rats
AIM: To investigate the effects of Hua Yu Dao Zhi decoction (HYDZD) on renal interstitial fibrosis induced by gentamicin in rats. METHODS: 32 healthy male Wistar rats were randomly divided into 3 groups: control group (n=6), gentamicin group (n=13) and HYDZD+gentamicin group (n=13). The renal tubulointerstitial fibrosis in rats was induced by gentamicin for 9 d. All rats were sacrificed on 30 d after modeling and renal tissues were stained with HE. The renal indexes were calculated and the changes of serum creatinine, blood urea nitrogen, total urinary protein in 24 h, and the level of α-SMA, Smad2, Smad7 were detected. RESULTS: Compared to control group, it was observed that there was mainly necrosis and partly degeneration in the renal tubular epithelial cells, proliferation of fibroblasts and infiltration of the inflammatory cells in the interstitial substance in gentamicin group under the light microscope. The renal indexes, serum creatinine, blood urea nitrogen, total urinary protein in 24 h, and the level of α-SMA, Smad2 were significantly higher than those in control group (P<0.01) and the level of Smad7 was significantly lower than that in control group (P<0.01). Under the light microscope, the results of all mentioned above in HYDZD+gentamicin group were improved significantly (P<0.01), the level of α-SMA, Smad2 were significantly lower than those in gentamicin group (P<0.01). However, no significant difference of Smad7 protein expression between gentamicin group and HYDZD+gentamicin group was observed (P>0.05). CONCLUSION: HYDZD is very effective in preventing and treating renal interstitial fibrosis caused by gentamicin with the decreased level of Smad2. 相似文献
10.
AIM: To explore the protective effect of fasudil hydrochloride against cisplatin(CP)-induced renal tubular epithelial cell apoptosis via Akt activation and PTEN inhibition. METHODS: Healthy male Sprague-Dawley (SD) rats were randomly divided into control group, CP group and CP+ fasudil group. All animals were sacrificed 96 h after injection of 0.9% saline or CP. Blood samples and kidney tissues were collected to evaluate levels of blood urea nitrogen (BUN), serum creatinine (sCr) and morphological alteration of the kidneys, respectively. The apoptosis of renal tubular epithelium cells was detected by TUNEL. Protein levels of Rho-associated protein kinase 1 (ROCK1), PTEN and Akt were measured by Western blotting and immunohistochemistry. The protein level of p-Akt was analyzed by Western blotting. RESULTS: Compared with control group, the sCr and BUN levels, the expression of ROCK1 and PTEN and TUNEL-positive cells were increased, while the level of p-Akt was decreased in CP group and CP+fasudil group. The histological structure of the kidneys observed by PAS staining was developed marked structural damage in CP group(P<0.05). Compared with CP group, sCr level, the expression of ROCK1 and PTEN and TUNEL-positive cells were decreased, while the level of p-Akt was increased in CP+fasudil group (P<0.05). Very little structural damage was detected in fasudil-treated groups. CONCLUSION: Fasudil hydrochloride has a protective effect on CP-induced renal tubular epithelial cell apoptosis via Akt activation and PTEN inhibition 1. 相似文献
11.
AIM: To observe the effects of astragalus polysaccharin (APS) on the expression of nephrin and podocin in podocytes of diabetic nephropathy (DN) rats.METHODS: The rat model of diabetes was induced by intraperitoneal injection of streptozotocin (STZ).The diabetic rats were randomly divided into 2 groups by the treatment without or with APS: STZ group (n=8) and STZ+APS group (n=8).In addition, 8 non-treated rats served as control.All the rats were treated with APS or normal saline orally by gavage for 8 weeks.The concentration of blood glucose was monitored on week 2, 5 and 8 after treatment.Eight weeks later, the body weight and renal index were measured.Total urine protein in 24 h, blood urea nitrogen (BUN) and serum creatinine (SCr) were detected by biochemical methods.The pathological changes of the kidneys were also observed under light microscope.The protein levels of nephrin and podocin in the kidney tissues were also determined by Western blotting.RESULTS: After APS intervention, the levels of renal index, blood glucose concentration, 24-hour total urine protein, BUN and SCr were significantly lower and body weight was higher than those in STZ group (P<0.05).The renal pathological status in APS group was significantly improved and the expression levels of nephrin and podocin also markedly increased (P<0.05).CONCLUSION: APS might protect kidney against STZ-induced injury via increasing the expression of nephrin and podocin in podocytes. 相似文献
12.
AIM: To explore the effects of rhynchophylline (RHL) on rat renal injury induced by adriamycin. METHODS: Fifty-two female SD rats were randomly divided into normal saline group (NSG, n=10), model group (MG, n=14), low-dose RHL treatment group (RHL-LG, n=14) and high-dose RHL treatment group (RHL-HG, n=14). The animals in the latter 3 groups were injected with adriamycin at a dose of 5 mg/kg through the tail vein. The animals in RHL-LG and RHL-HG were treated with RHL at doses of 5 mg/kg and 15 mg/kg, respectively, by intraperitoneal injection for 8 weeks. The animals in NSG and MG were treated with normal saline only. Urine and blood samples were collected to detect the urine protein, blood urea nitrogen (BUN) and serum creatinine (SCr). The renal tissues of the animals were collected for detection of malondialdehyde (MDA) content, superoxide dismutase (SOD) activity, pathological changes and mRNA expression of angiotension Ⅱreceptors 1,2 (AT1,2-R), angiotensin-converting enzyme (ACE) and angiotensinogen (AGT). RESULTS: The urine protein, BUN and SCr in RHL-LG were significantly lower than those in MG, but higher than those in RHL-HG (P<0.05). The SOD activity in MG was significantly lower than that in RHL-LG in the renal tissues. The SOD activity in RHL-LG was significantly lower than that in RHL-HG (P<0.05), but the content of MDA was on the contrary. The renal pathological damages in RHL-LG were weaker than that in MG, and that in RHL-HG were weaker than that in RHL-LG. The mRNA expression of AT1-R, ACE and AGT in MG was significantly higher than that in RHL-LG in the renal tissues, and that in RHL-LG was higher than that in RHL-HG (P<0.05). The mRNA expression of AT2-R in MG was significantly lower than that in RHL-LG, and that in RHL-LG was significantly lower than that in RHL-HG (P<0.05). CONCLUSION: RHL reduces adriamycin-induced renal injury in rats by attenuating the injury of lipid peroxidation in renal tissue, regulating the mRNA expression of AT1, 2-R, ACE and AGT, and increasing renal blood flow. 相似文献
13.
AIM:To explore the effect of pidotimod on the renal function in IgA nephropathy (IgAN) rat model, and to further study whether this effect is related to the inhibition of inflammatory response. METHODS:The SD rats (n=36) were randomly divided into control group, IgAN model group, IgAN with prednisone treatment group and IgAN with pidotimod treatment group, with 9 rats in each group. The IgAN model was induced by consecutive oral administration of bovine gamma globulin (BGG) for 8 weeks followed by injection of BGG through tail vein for 3 d. After the IgAN model was established, the drug was continuously used for 4 weeks. At the end of the treatment, the urine protein, serum creatinine and blood urea nitrogen were examined by an automated analyzer. IgA deposition in the renal tissues was observed by immunofluorescence staining. The mRNA expression levels of renal fibrosis markers transforming growth factor-β1 (TGF-β1) and fibronectin 1 in the renal tissues were detected by RT-qPCR. The mRNA and protein levels of pro-inflammatory cytokines interleukin-1β (IL-1β) and IL-6 in the renal tissues were determined by RT-qPCR and Western blot, respectively. RESULTS:No significant difference of the body weight was observed in different groups. Compared with control group, the content of urine protein, serum creatinine and blood urea nitrogen were significantly increased (P<0.01), whereas those were reversed by pidotimod treatment. The results of immunofluorescence staining showed that pidotimod inhibited IgA deposition in the IgAN rats. Pitomod treatment inhibited the mRNA expression levels of renal fibrosis markers TGF-β1 and fibronectin 1, and the mRNA and protein levels of pro-inflammatory cytokines IL-1β and IL-6 in the renal tissues of IgAN rats. CONCLUSION:Pidotimod alleviates IgAN progression in rats by inhibition of inflammatory response. 相似文献
14.
AIM: To investigate the effects of sesamin on progression of renal injury in renal hypertensive and hyperlipidemic rats (RHHR). METHODS: RHHR was induced by 2K1C and high lipid baitvessel. After 7 weeks of intragastric administration with sesamin, the contents of serum creatinine (Scr), blood urea nitrogen (BUN), 24 h urinary protein excretion (UPE) were measured. In addition, the activity of total antioxidative capacity (T-AOC), superoxide dismutase (SOD), the concentrations of malondialdehyde (MDA), hydrogen peroxide (H2O2), and the nitric oxide synthase (NOS) and nitric oxide (NO) levels in renal homogenate were measured. RESULTS: Compared with the model group, seasamin (in 100 mg·kg-1 and 33 mg·kg-1 groups) evidently decreased the contents of Scr, BUN, UP and the concentration of MDA, iNOS, H2O2 in renal tissure. It also improved the levels of NO, cNOS and activity of SOD, T-AOC in renal tissure. CONCLUSION: Sesamin ameliorates hypertensive and hyperlipidemic-induced renal injury, probably by enhancing antioxidative activity, scavenging hydroxyl radical and restraining iNOS level. 相似文献
15.
LI Min HUANG Ting-ting JIANG Shuang-yan YANG yang CHEN Sha-sha ZHANG Wen-jia ZHAO Li-juan 《园艺学报》2019,35(11):2050-2054
AIM: To investigate whether curcumin reduces hepatocyte apoptosis in the rats with non-alcoholic steatohepatitis (NASH) by inhibiting endoplasmic reticulum stress (ERS) and thus exerting a protective effect on the liver. METHODS: Male SD rats (n=30) were randomly divided into normal control group (n=10), model group (n=10) and curcumin group (n=10). NASH model was established by feeding the rats with high-fat diet for 4 weeks. The rats in curcumin group was given curcumin (200 mg/kg) daily by gavage, while the rats in model group and normal control group were given the same volume of saline. Four weeks later, the rats were killed, and their blood and liver tissues were collected. The serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were detected, liver histopathological changes were observed by HE staining, the expression of glucose-regulated protein 78 (GRP78) and C/EBP homologous protein (CHOP) was determined by Western blot, and apoptosis was detected by TUNEL method. RESULTS: Compared with normal control group, the levels of serum ALT and AST in model group were significantly increased, and the levels of serum ALT and AST in curcumin group were significantly lower than those in model group (P<0.05). At the same time, the steatosis and inflammation of hepatocytes in curcumin group were less than those in model group, and no obvious necrosis was observed. Compared with normal control group, the protein expression levels of GRP78 and CHOP in model group were increased, while the protein expression levels of GRP78 and CHOP in curcumin group were decreased compared with model group (P<0.01). TUNEL results showed that apoptotic hepatocytes in model group were significantly more than those in normal control group, while those in curcumin group were significantly fewer than those in model group. CONCLUSION: Hyperlipidemia induces excessive ERS in the hepatocytes, thus triggering apoptosis and leading to NASH. The mechanism of curcumin reducing hepatocyte apoptosis may be related to its inhibition of ERS. 相似文献
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AIM: To study the protective effect of aqueous extract of 2-branched and 3-branched velvet antler on cisplatin (CDDP)-induced nephrotoxicity in mice. METHODS:The mouse model of renal injury was induced by intragastric administration of CDDP at the dose of 15 mg/kg. After treatment, kidney index (KI), serum creatinine (SCr), blood urea nitrogen (BUN), the activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), and the content of malondialdehyde (MDA) in the kidney were determined. The renal pathological changes were observed with HE staining. RESULTS: Aqueous extract of velvet antler at the tested dose markedly decreased BUN, SCr and the content of MDA, and elevated the activity of SOD and GSH-Px in the mice pretreated with CDDP (P<0.05). The pathological changes of the renal tissues were improved obviously, and the injury of the epithelial cells of renal tubules was mitigated. The effect of the aqueous extract of 2-branched velvet antler on renal function and cisplatin-induced nephrotoxicity was better than that of 3-branched one at the same concentration. CONCLUSION: The aqueous extract of 2-branched and 3-branched velvet antler has a certain protective effect on cisplatin-induced nephrotoxicity, which may be associated with increasing the anti-oxidative capability of mouse renal tissue. 相似文献
18.
ZHANG Da LI Shu-yu WANG Yan-fei LI Yi-xuan YI Yue-e GAO Yu-shan ZHANG Shu-jing JIA De-xian WANG Qian 《园艺学报》2017,33(1):166
AIM: To investigate the effects of astragalus injection combined with puerarin injection on endoplasmic reticulum stress through PERK pathway in diabetic nephropathy mice. METHODS: Male KKAy mice were randomly divided into model group (injected with normal saline) and treatment group (injected with astragalus and puerarin). The male C57BL/6J mice served as normal group. The mice were sacrificed 4 weeks after treatments for observing morphological changes under electron microscope. The renal tissues were collected to determine the expression of protein kinase R-like endoplasmic reticulum kinase (PERK), eukaryotic initiation factor 2α (eIF2α) and glucose-regulated protein 78 (GRP78) at mRNA and protein levels by real-time PCR and Western blot. RESULTS: Under electron microscope, the renal tubular epithelial cells in model group and treatment group showed the swelling of the nucleus, endoplasmic reticulum and mitochondria. The results of real-time PCR and Western blot showed that the expression of PERK, eIF2α and GRP78 at mRNA and protein levels in model group was higher than that in normal group (P<0.05), while that in treatment group was lower than that in model group. CONCLUSION: Astragalus injection combined with puerarin injection reduces the mRNA and protein expression of PERK, eIF2α and GRP78, thus inhibiting the endoplasmic reticulum stress in type 2 diabetic mice to protect the kidney function. 相似文献
19.
ZHANG Chun-jing ZHANG Yue WANG Xiao-long GUO Hong-yan XU Jing LI Shu-yan ZHAO Zheng-lin 《园艺学报》2018,34(12):2289-2293
AIM:To observe the effects of ginsenoside Rh1 (G-Rh1) on unilateral ureteral obstruction (UUO)-induced renal interstitial fibrosis and to investigate the underlying mechanisms. METHODS:Male Sprague-Dawley (SD) rats (n=40) were divided into the following 4 groups:UUO-operated group (UUO group), sham-operated group (sham group), UUO-operated plus a low dose (50 mg·kg-1·d-1) of G-Rh1 treatment (low G-Rh1 group) and UUO-operated plus a high dose (100 mg·kg-1·d-1) of G-Rh1 treatment group (high G-Rh1 group). The G-Rh1 treatment was carried out by gastric gavage from the next day after the UUO operation once a day for 2 weeks (14 d). Immediately after the final dose of G-Rh1, 24 h urine was collected for the urine protein test, and then the rats were euthanized. The blood was collected for the blood urea nitrogen (BUN) and serum creatinine (SCr) assays, and the kidney was removed for pathological and biochemical evaluations. RESULTS:The levels of 24 h urine protein did not show any significant diffe-rence among the groups, while significantly increased levels of BUN and SCr in UUO group were observed (P<0.05), which was prevented by the treatment with G-Rh1 at both doses in a dose-dependent manner. Pathological evaluation showed the renal tissue damage was obvious in UUO group, which was improved by the treatment with G-Rh1 at both doses. Immunohistochemcial analysis exhibited that UUO increased renal interstitial transforming growth factor-β1 (TGF-β1) expression, which was also inhibited by the treatment with G-Rh1 at both doses(P<0.05). Significantly increased protein expression of renal interstitial collagen type I, α-smooth muscle actin (α-SMA) and connective tissue growth factor (CTGF) in UUO group was detected, which was suppressed by the treatment with G-Rh1 at both doses. CONCLUSION:G-Rh1 improves UUO-induced renal dysfunction and attenuates interstitial fibrosis, which is mediated via modulation of TGF-β1-related pro-fibrogenic signaling pathway. 相似文献
20.
AIM: The different effect of bivalent immunoglobulin Yolk (IgY) was evaluated against snake venom between intragastric administration and intraperitoneal injection in mice with cobra or viper envenomation. METHODS: The venom of naja and viper was injected alternately into the leghorn hen. Bivalent anti-snake venom IgY was extracted by water dilution. The concentration of bivalent IgY in plasma was observed in indirect ELISA assay after bivalent anti-snake venom IgY taken orally. The gastric emptying function test was used for determining optimization time after gastric administration of IgY. The protective effect of bivalent anti-snake venom IgY was compared between intragastric administration and intraperitoneal injection in mice with cobra or viper envenomation. RESULTS: Bivalent anti-snake venom IgY was extracted from eggs laid in 28-42 d after the first immunization. The titers of Bivalent IgY against cobra and viper venom were 1∶12 800 and 1∶6 400. At the time of 2.5-3.5 h after bivalent anti-snake venom IgY was taken orally in three concentrations (75 mg, 150 mg, 300 mg·0.5 mL-1·20 g-1 BW), the gastric evacuation rate of mice was above 68.9%, with the plasma concentration of bivalent IgY in peak. The survival time of mice envenomation with snake venom was extremely prolonged (P<0.01), after IgY was taken by intragastric administration or intraperitoneal injection. While administration with the same dose of IgY, the survival rate of mice envenomation with cobra venom was higher than that of viper venom. The effective dose of intragastric administration was decuple higher than that of intraperitoneal injection. CONCLUSION: The animal envenomation with cobra or viper venoms can be significantly protected by bivalent anti-snake venom IgY with intragastric administration or intraperitoneal injection. 相似文献