—大白菜干烧心病发生过程中心叶组织Ca^2＋定位及超微结构变化   总被引：7，自引：2，他引：5  

缪颖  蒋有条 《园艺学报》1997,24(2):145-149

采用电镜细胞化学技术，研究了人为诱导大白菜干烧心病发生过程中心叶组织细胞的Ｃａ２＋定位和超微结构变化。结果表明，正常植株叶片细胞内钙主要分布于液泡、细胞壁和叶绿体的膜片层结构中；试验处理植株随着缺钙天数的增加，细胞壁膜上的钙被释放沉淀到细胞间隙，叶绿体片层膜上的Ｃａ２＋释放到胞液中。缺钙后期，细胞内钙沉淀明显减少。同时细胞结构改变，质膜内陷，叶绿体膜和内膜系统破坏；随后细胞壁中胶层解体，可见症状出现时，细胞内隔消失，细菌侵入细胞间隙  相似文献   

低温胁迫下北海道黄杨叶肉细胞Ca²⁺和Ca²⁺-ATPase的变化     

杨蕊  关雪莲  张睿鹂  杨文莉  郑健  冷平生 《园艺学报》2013,40(6):1139

 利用焦锑酸钙沉淀和硝酸铅沉淀的电镜细胞化学方法,以室温生长的北海道黄杨植株为对照,研究了人工4 ℃低温胁迫过程中北海道黄杨（Euonymus japonicus‘Cuzhi’）叶肉细胞Ca²⁺和Ca²⁺-ATPase的动态变化。在4 ℃低温胁迫的初期（3 ~ 12 h）,北海道黄杨叶肉细胞间隙和液泡内的Ca²⁺沉淀颗粒减少,而细胞质和细胞核内的Ca²⁺水平升高,但Ca²⁺-ATPase在细胞的分布几乎没有变化,主要分布在质膜和液泡膜上,有较高的活性;低温胁迫24 h,细胞质和细胞核内增加的Ca²⁺开始回到细胞间隙和液泡中,Ca²⁺-ATPase在质膜和液泡膜上活性增强;在低温胁迫48 ~ 96 h,细胞内的Ca²⁺又回到低温胁迫前的低水平,但Ca²⁺-ATPase在质膜和液泡膜上仍有很高的活性。叶肉细胞内Ca²⁺稳态平衡和Ca²⁺-ATPase的活性变化与植物的抗寒性存在一定的相关性。  相似文献   

高温锻炼和水杨酸预处理对热激下葡萄叶肉细胞超微结构的影响   总被引：5，自引：1，他引：5  

刘悦萍  黄卫东  张俊环 《园艺学报》2006,33(3):491-494

 以‘京秀’葡萄(Vitis vinifera L. ‘Jingxiu’) 幼苗为试材, 研究了高温锻炼或水杨酸预处理对热激后叶肉细胞超微结构的影响。结果表明, 热激后细胞结构明显被破坏, 高温锻炼预处理对叶肉细胞造成了轻微的伤害, 但延缓了之后的热激对叶肉细胞超微结构的进一步伤害, 主要表现在质膜、液泡膜、细胞核和叶绿体等部位。水杨酸预处理对叶肉细胞结构无明显影响, 并在之后的热激下细胞结构的受伤程度远低于蒸馏水处理。这说明, 高温锻炼或水杨酸预处理保持了叶肉细胞结构在高温下的稳定性, 从而提高了葡萄幼苗的抗热性。  相似文献   

苹果叶片受斑点落叶病菌侵染过程中细胞Ca2+定位及MdCPK的表达     

魏萌涵  倪维晨  竹龙鸣  蔡斌华  王三红 《园艺学报》2015,42(11):2113-2122

以苹果感病品种‘红星’和抗病品种‘红玉’为材料,研究了苹果与斑点落叶病菌（Alternaria alternata apple pathotype）互作过程中超微结构,细胞Ca2+分布,以及在钙信号转导途径中具有重要作用的钙依赖蛋白激酶基因（CDPK）的表达,探讨钙信号在苹果防御斑点落叶病菌侵染中的作用。结果表明,在未接种状态下,叶肉细胞结构完好,叶绿体呈卵圆形沿细胞边缘排列,Ca2+主要分布在细胞间隙和液泡中,‘红玉’细胞间隙Ca2+密度比‘红星’大。接种斑点落叶病菌8 h,‘红星’叶肉细胞中的Ca2+沉淀主要分布在胞质中,而液泡等细胞器中减少;‘红玉’的Ca2+沉淀主要集中在胞质和筛管分子中,液泡和细胞间隙中减少,且趋向于在细胞壁外围和液泡膜上沉积。接种18 h,‘红星’叶肉细胞间隙Ca2+沉淀密度增加,而‘红玉’中的Ca2+沉淀主要集中在叶肉细胞的胞质和液泡,以及筛管分子中。接种24 h,‘红星’叶肉细胞结构已发生形变,质膜发生裂解,筛管壁木质化加厚,Ca2+沉淀主要分布在液泡中;‘红玉’叶片细胞结构完好,Ca2+沉淀主要分布在液泡和胞质中。接种36 h,‘红星’叶肉细胞受到菌丝入侵,结构和形态遭到破坏,在未受损叶肉细胞中Ca2+沉淀主要集中在液泡中,在受损的细胞中Ca2+沉淀无序地散布在受损细胞周围及细胞间隙;此时‘红玉’叶肉细胞中Ca2+沉淀主要集中在胞质和液泡中,并且能够保持Ca2+动态平衡。在接种后不同阶段,‘红星’和‘红玉’叶片中MdCPKs基因呈现不同的表达特点：大多MdCPKs在‘红玉’中的表达量在24 h达到最高值;‘红星’中在36 h达到表达峰值,且表达量也比‘红玉’中低得多。上述结果表明,钙信号响应斑点落叶病菌侵染,在抗病苹果品种‘红玉’中,Ca2+内流是细胞质Ca2+上升的主要来源;在感病品种‘红星’中,细胞器Ca2+释放是细胞质Ca2+的主要来源。‘红玉’苹果MdCPKs基因响应病菌侵染比‘红星’苹果早而且强烈。  相似文献   

茉莉幼苗ATP 酶活性的超微细胞化学定位与耐冷性研究   总被引：9，自引：0，他引：9  

苏金为  王湘平 《园艺学报》2001,28(6):544-550

 采用磷酸铅沉淀的细胞化学方法, 电镜下观察两个抗冷力显著不同的茉莉品种幼苗ATP 酶活性在细胞中超微结构定位及其经冷胁迫的变化。结果表明, 抗寒力强的双瓣茉莉品种比抗寒力弱的单瓣品种在低温胁迫下超微结构和ATP 酶活性有更强的适应能力。茉莉幼苗叶肉组织ATP 酶主要定位于细胞质膜、液泡膜、叶绿体片层、线粒体脊和胞间连丝等部位, 小叶脉维管束的ATP 酶主要分布在筛管和伴胞。  相似文献   

黄瓜“花打顶” 的组织和细胞学特征     

李志英  李兴国  徐立  郑成超 《园艺学报》2004,31(3):315-320

 通过解剖学和细胞学的研究发现，黄瓜“花打顶”茎尖部位的节数、节间长度和茎粗均减小，顶端幼茎细胞的伸长和伸展受到抑制，维管束数目增加，导管壁和厚角组织细胞壁明显增厚，顶端分生组织分化的原基数量相对减少，并簇生，顶端分生组织的体积减小，细胞液泡化，核膜、液泡膜及质体膜等部分降解，细胞呈现衰老趋势；进一步利用mRNA差异显示技术获得了黄瓜生长正常和花打顶的差异表达cDNA片段，Northen分析表明其中精氨酸脱羧酶(ADC)的同源基因和叶绿体ATP~e亚基III的同源基因分别属黄瓜花打顶的正调控和负调控基因。  相似文献   

低温逆境下两个抗寒性不同的核桃幼叶Ca2+的亚细胞定位的变化     

田景花  王红霞  张志华  高仪 《园艺学报》2013,40(3)

为揭示核桃的抗寒机理,研究了低温逆境下不同抗寒性核桃(Juglans regiaL.)品种展叶期叶片细胞中第二信使Ca2+的分布变化.试验材料为抗寒性较强的核桃品种‘哈特雷’和抗寒性较差的‘晋龙2号’展叶期1年生枝的顶端幼叶,将枝叶于1℃低温下分别水培处理3、12、24、48和72 h,以未经低温处理的叶片作为对照,采用电镜细胞化学方法,定位观察叶肉细胞中的Ca2+分布.结果表明,展叶期核桃幼叶细胞中Ca2+主要分布于液泡和细胞间隙中,线粒体、叶绿体和细胞核中也有较多Ca2+分布,基础细胞质中Ca2+含量较少.1℃低温可使细胞质和细胞核中的Ca2+浓度迅速升高,在低温处理24h时,抗寒性较强的‘哈特雷’细胞中游离Ca2+浓度开始下降,处理72 h时基本恢复到静息态水平,细胞超微结构变化不大,叶片无明显冷害症状;而抗寒性较差的‘晋龙2号’叶片的细胞质、线粒体及细胞核中Ca2+浓度始终处于较高水平,到处理72 h时细胞超微结构冷害明显,部分幼叶叶缘呈水浸状.可见,低温逆境下抗寒性较强的品种叶肉细胞质中出现短时间的Ca2+高峰,随后能恢复到静息态水平;而抗寒性差的品种难以恢复,不能完成第二信使的信号传导过程,最终导致细胞和组织冷害.因此,低温逆境下能否恢复第二信使Ca2+的稳定平衡与核桃的抗寒性密切相关.此外,低温处理12 h后核桃幼叶的叶绿体被膜上普遍出现Ca2+沉淀,推测此时叶绿体可能起着临时贮存Ca2+的作用,以促进细胞质中Ca2+浓度回落到静息态水平;线粒体可能也具有此作用.  相似文献   

黄瓜叶片可产生胞质脂滴     

《中国瓜菜》2019,(4):35-40

胞质脂滴通常在生殖器官中产生,用透射电子显微技术研究黄瓜叶片能否产生胞质脂滴,哪种类型的细胞能产生脂滴,以为黄瓜叶片脂滴的开发研究提供参考。结果表明,幼叶、成熟叶和衰老叶都含有胞质脂滴,即黄瓜叶片发育各阶段均能产生胞质脂滴。幼叶中,表皮细胞、叶肉细胞和维管组织细胞都含有少量胞质脂滴;成熟叶中,以叶肉细胞中胞质脂滴较多;老叶中,叶肉细胞、维管薄壁细胞和伴胞都产生胞质脂滴,以伴胞中最多。老叶叶肉细胞中胞质脂滴向液泡内转移,显示胞质脂滴通过细胞自噬途径降解。  相似文献   

黄瓜叶片衰老过程中脂滴的原位降解和液泡内降解     

葛姝君  赵丹  周雪  孙晴  刘林 《园艺学报》2016,43(10):2021-2028

对黄瓜不同发育阶段叶片中的脂滴进行超微观察,以期了解衰老叶内脂滴降解的场所。结果表明,衰老叶片中部分质体小球在叶绿体内降解,降解先从小球一侧开始,向另一侧推进。衰老叶片含有胞质脂滴,有些胞质脂滴紧贴叶绿体,显示其发生与叶绿体密切相关;有些胞质脂滴与液泡密切接触,并向液泡内转运,进入液泡后发生降解。显然,黄瓜叶衰老不仅引起脂滴在液泡内降解,也引起脂滴在叶绿体内原位降解,证明液泡并不是脂滴降解的唯一场所。  相似文献   

板栗短雄花序异常死亡的超微结构观察   总被引：5，自引：1，他引：4  

张婧  董清华  杨凯  曹庆芹  冯永庆  秦岭 《园艺学报》2007,34(3):605-608

板栗(Castanea mollissima Bl. ) 短雄花序发育过程中, 花序上部变黄, 弯曲, 最后枯死脱落,而基部花序正常发育。利用透射电镜观察花序上部发育中的细胞超微结构发现: 细胞呈现有序的死亡变化,细胞核内发生染色质凝聚, 继而核仁消失, 核质降解, 核膜破裂, 核解体; 液泡内吞现象明显, 形成很多小液泡; 叶绿体和线粒体也逐渐解体; 花序上部衰亡过程中所有细胞器在膜包裹下有序地降解, 表现出植物细胞程序性死亡的典型形态学特征。  相似文献   

CaM在梨花芽分化过程中的含量变化   总被引：8，自引：0，他引：8  

彭抒昂  罗充 《园艺学报》1998,25(3):220-223

以梨为试材，对成花过程中的短枝芽和叶所含钙调素（ＣａＭ）的含量进行了测定。（１）短枝芽的ＣａＭ含量在整个成花前后都明显高于新梢芽的含量，特别是在成花的发端期突然成倍增加，形成高峰；（２）短果枝叶的ＣａＭ与新梢叶有着相近的变化趋势，但在成花前后，短果枝叶的ＣａＭ含量明显高于新梢叶；（３）ＣａＡｃ处理可以使短枝芽所含ＣａＭ的峰值提前出现，ＴＦＰ处理可明显降低ＣａＭ含量和延迟其峰值到来。还就ＣａＭ在成花过程中的作用及与Ｃａ２＋的关系等问题进行了讨论。  相似文献   

甘蓝花粉管钙感应蛋白CaM 与SRK 相互作用研究   总被引：2，自引：0，他引：2  

许俊强  孙梓健  宋明  汤青林  王志敏  王小佳 《园艺学报》2013,40(12):2429-2440

 为研究甘蓝花粉管钙感应蛋白CaM 与SRK 相互作用的分子机理及其可能相互作用的区域, 从自交不亲和甘蓝材料‘E1’中分别克隆得到CaM12 基因450 bp 及S 位点受体激酶（SRK7）基因全长 序列2 118 bp,并亚克隆得到SRK 胞外域（eSRK）和胞内激酶域（iSRK）,构建原核表达载体pGEX-CaM12、 pCold-eSRK 和pCold-iSRK,转化E. coli BL21（DE3）进行原核表达,表达产物纯化后进行体外相互作用, 结果表明CaM12 能够与SRK7 进行相互作用,但作用区域是iSRK7 而不是eSRK7。为进一步验证其相互 作用,本研究利用酵母双杂交系统,构建pGBKT7-CaM12、pGADT7-eSRK7、pGADT7-iSRK7 和 pGADT7-SRK7 酵母表达载体,转化相应酵母Y2HGold 和Y187 感受态细胞后未出现自激活和毒性现象, 相互作用结果与原核表达检测一致。同时将CaM12 的3 个EF-hands 结构域突变体CaM12-2-、CaM12-23- 和CaM12-234-与iSRK7 分别构建酵母表达载体pGADT7-CAM12-2-、pGADT7-CAM12-23-、pGADT7- CAM12-234-,检测其相互作用。结果表明CaM12 EF-hands 突变体CaM12-2-、CaM12-23-和CaM12-234- 在酵母双杂交系统中均不能与iSRK7 片段发生相互作用,说明CaM12 的EF-hands 结构域突变后失去结 合Ca2+能力而不能与iSRK7 相互作用。该研究可为自交不亲和机理提供新的参考依据。 关键  相似文献   

Effect of Astragalus injection on expression of calmodulin after hypoxia/hypoglycemia and reoxygenation in rat hippocampal neurons     

YAN Feng-xia  QIAN Tao  GAO Wei-juan  YE Dong-qing  REN Li-qun  ZHANG Ya-li  HOU Zhi-ping 《园艺学报》2011,27(9):1708-1714

AIM: To investigate the effect of Astragalus injection on the expression of calmodulin(CaM) after hypoxia/ hypoglycemia and reoxygenation in rat hippocampal neurons.METHODS: The hippocampal neurons were cultured for 8 days and divided into 4 groups: normal control group (normal control), hypoxia/hypoglycemia and reoxygenation group (model), Astragalus injection solution group (solution control) and Astragalus injection group ( Astragalus ).The cells in all groups were treated with reoxygenation and normal medium after deprived of oxygen and glucose for 30 min except normal control group.The method of immunohistochemistry was used to measure the number of caspase-3 positive neurons.The expression of CaM at mRNA and protein levels was measured at time points of 0 h, 0.5 h, 2 h, 6 h, 24 h, 48 h, 72 h and 120 h after hypoxia/hypoglycemia and reoxygenation by RT-PCR and Western blotting, respectively.RESULTS: No difference of the parameters at all time points between model group and solution control group was found.Compared with normal control group, the numbers and the percentages of caspase-3 positive cells at all time points obviously increased in model group except at 0 h and 0.5 h (P<0.05).Compared with model group, the numbers and the percentages of caspase-3 positive cells were decreased in Astragalus injection group except at 0 h and 0.5 h (P<0.05).Compared with normal control group, the protein expression of CaM in rat hippocampal neurons at all time points obviously increased in model group (P<0.05).However, the protein expression of CaM in rat hippocampal neurons at all time points obviously decreased in Astragalus injection group as compared with model group (P<0.05).Compared with normal control group, the mRNA expression of CaM in rat hippocampal neurons at all time points obviously decreased in model group (P<0.05).The mRNA expression of CaM in rat hippocampal neurons at all time points obviously increased in Astragalus injection group as compared with model group (P<0.05).CONCLUSION: Astragalus injection inhibits the protein expression of CaM, the calcium overload and the expression of caspase-3 after hypoxia/hypoglycemia and reoxygenation, thus inhibiting hippocampal neuronal apoptosis.  相似文献   

钙调素对梨自花及异花授粉后花柱自发荧光变化的影响   总被引：2，自引：0，他引：2  

周建涛  姜雪婷  刘珠琴  张绍铃 《园艺学报》2008,35(6):781-786

采用激光共聚焦显微镜研究了外源钙调素及其抗血清对‘丰水’梨自花（不亲和）和异花（亲和）授粉后花柱自发荧光变化的影响。结果表明：授粉12 h后,CaM处理后异花授粉花柱荧光值与未处理异花授粉花柱荧光值相比,花柱上部荧光值降低下部升高;而CaM处理后自花授粉花柱自发荧光值除距柱头2 500～3 000 μm外其余部分均高于未处理自花授粉花柱;CaM抗血清处理后异花授粉花柱荧光值除距柱头1 000～2 000 μm外其余部分均高于未处理异花授粉花柱;CaM抗血清处理自花授粉花柱自发荧光与未处理自花授粉花柱相比,分布规律发生改变,最高值位于距柱头1 000～1 500 μm处。授粉72 h后CaM处理后异花授粉花柱荧光值高于未处理异花授粉花柱,最高值位于子房端;CaM处理后自花授粉与未处理自花授粉花柱自发荧光值相比,分布规律发生改变,最高值位于距柱头1 500～2 000 μm处;CaM抗血清处理后异花及自花授粉花柱自发荧光与未处理异花及自花授粉花柱相比较分布规律均发生了改变,最高值都位于0～500 μm处。证实了外源CaM及其抗血清对自花、异花授粉的生理代谢过程均有影响。  相似文献   

结球甘蓝花粉钙调素基因的克隆与表达分析   总被引：1，自引：1，他引：0  

孙梓健  韦静宜  王小佳  宋明  汤青林  王志敏  任雪松 《园艺学报》2012,39(4):677-686

 根据芸薹属植物钙调素基因保守区域设计引物,采用同源克隆的方法从结球甘蓝自交不亲和系和自交系花粉中克隆得到一个钙调素开放阅读框cDNA 序列,该序列长450 bp,编码149 个氨基酸;编码蛋白不含跨膜区,无信号肽,具有4 个完整的EF-hand 结构域。构建了结球甘蓝花粉钙调素原核表达系统,钙调素基因及其3 个突变体在E. coli 中得到表达,均获得分子量约为16 kD 的可溶性融合蛋白,在EGTA 存在的条件下,各融合蛋白具有各自独特的凝胶迁移现象,活性检测表明甘蓝花粉钙调素活性依赖于钙离子。该基因在甘蓝自交不亲和系花粉萌发过程中表达量先上升后下降,在自交系中随花粉萌发增大而降低;在含有钙调素拮抗剂TFP 的培养基中,自交不亲和系和自交系花粉中钙调素基因表达量均受到抑制;在含有W-7 琼脂糖的培养基中无明显差异。  相似文献   

ATPase activities and Ca²⁺/calmodulin alterations in hippocampi of rats with PTSD-like behaviors     

WANG Qing-song  WANG Zheng-guo  ZHU Pei-fang 《园艺学报》2002,18(9):1046-1049

AIM: To explore the pathophysiological bases in the pathogenesis of the lasting emotional behavioral disorders following posttraumatic stress disorder(PTSD). METHODS: 240 male Wistar rats were divided randomly into 3 groups. Group SE(n =96) for rats with PTSD-like behavior by constant pulsating current of 100 μA with intratrain frequencies of 16 Hz, pulsating duration of 1 ms, train duration of 10 s and interstimulus interval of 7 min for 5 days with 8 times per day. Group CE(n =96) for control with electrode implanted in hippocampus without stimulation, and Group NC(n =48) for normal control. The activities of Na⁺-K⁺-ATPase and Ca²⁺ -ATPase, levels of intracellular calcium and free calmodulin(CaM), and the total CaM expression were detected in hippocampi of experimental rats. RESULTS: The activities of Na⁺-K⁺-ATPase and Ca²⁺ -ATPase in mitochondria of hippocampal cells in Group SE rats were significantly decreased at 48 h and 72 h after the last stimulation, respectively. The intracellular free calcium levels were increased, and the mean channel fluorescence of intracellular free CaM decreased remarkably at 72 h poststimulation, while the expression of total CaM was significantly elevated at 48 h after the last stimulation in hippocampi of Group SE rats. CONCLUSION: The lasting increased levels of intracellular free calcium and expression of Ca²⁺ -CaM in hippocampus, as well as the dysfunction of Na⁺-K⁺ pump and Ca²⁺ -ATPase in mitochondria may play important roles in the long-term neuropsychological sequelae in PTSD.  相似文献   

Effects of lentivirus-mediated transfection of shRNA targeting Adra1d gene on calcium and calmodulin in rat aortic vascular smooth muscle cells     

ZHAO Liang-yuan  HOU Xiao-min  QIN Xiao-jiang 《园艺学报》2018,34(7):1306

AIM:To investigate the effects of lentivirus-mediated transfection of shRNA targeting α_1D-adrenergic receptor (Adra1d) gene on calcium ion (Ca²⁺) and calmodulin (CaM) in vascular smooth muscle cells (VSMCs) of rat aorta. METHODS:Single oligonucleotide sequences of shRNA targeting rat Adra1d gene were design and synthesized, and then the shRNA was constructed and cloned into GV248 vector. The U6-shRNA carrier and expression vector were transfected into 293T cells together and packed with lentivirus, and the supernatant was collected and concentrated by overspeed centrifugation. The VSMCs of rat aorta were transfected with recombinant lentivirus vector. The interference effects were identified by RT-qPCR and Western blot. The concentration of Ca²⁺ in VSMCs was detected by laser confocal inspection, and the expression of CaM at mRNA and protein levels in the VSMCs was determined by RT-qPCR and Western blot. RESULTS:The lentiviral shRNA expression vector was successfully constructed. The titer of the concentrated virus was 3×10¹¹ TU/L. The mRNA and protein expression levels of Adra1d in the rat aortic VSMCs were significantly reduced after transfection. The interference efficiency of Lv-shRNA4-Adr to Adrald gene was greater than 85%. After target silencing of Adra1d gene, compared with scrambled group, the Ca²⁺ fluorescence intensity of rat aortic VSMCs was significantly increased. Moreover, the mRNA and protein expression levels of CaM were also increased significantly. CONCLUSION:A lentiviral shRNA expression vector targeting rat Adra1d gene was successfully constructed, which significantly increased Ca²⁺ concentration and CaM expression in rat aortic VSMCs.  相似文献   

草莓和番茄果实乙烯自我催化与Ca²⁺-CaM 的关系   总被引：3，自引：1，他引：3  

顾采琴  席玙芳  关军锋  李广敏 《园艺学报》2003,30(3):263-266

 外源乙烯处理乳白期草莓和绿熟期番茄果实12 h后，诱导草莓乙烯大量合成，使番茄乙烯释放高峰提前2 d出现，同时还促进两种果实的钙调素(Calmodulin，CaM)含量增加。细胞质膜钙离子通道阻塞剂异搏定(Verapamil，Vp)、钙调素拮抗剂氯丙嗪(Chloropromaize，CPZ)和三氟拉嗪(Trifluoperazine，TFP)均抑制了外源乙烯诱导的草莓乙烯合成，表明Ca²⁺-CaM 信使系统可能参与草莓乙烯自我催化作用；Vp抑制外源乙烯诱导的番茄乙烯合成，而CPZ和TFP的作用不显著，说明番茄果实乙烯自我催化作用与胞外Ca²⁺ 内流有关，与CaM的关系不明显。  相似文献   

Ca~（2＋），CaM与花朵衰老的关系   总被引：2，自引：0，他引：2  

郑海金  陈鑫阳  杨振土  张昀  张微  张慧 《园艺学报》1994,(3)

对君子兰（ＣｌｉｖｉａｍｉｎｉａｔａＲｅｇ．）、牡丹（ＰａｅｏｎｉａｓｕｆｆｒｕｔｉｃｏｓａＡｎｄｒ．）、令箭荷花（ＮｏｐａｌｘｏｃｈｉａａｃｋｅｒｍａｎｎｉｉＨａｗ．）等三种不同寿命的花，在盛开与衰老时的可溶性总蛋白、内源总Ｃａ２＋水平以及总ＣａＭ含量进行了对比试验。结果表明，三种花衰老时可溶性总蛋白含量均下降，但长寿花比短寿、中寿花含有更高水平的蛋白质，并且水解速率较低；三者都有较高的内源总Ｃａ２＋及ＣａＭ水平。这些提示内源Ｃａ２＋、ＣａＭ水平与花的寿命有一定的相关性。  相似文献   

Analgesic effect of angiotensin angiotensin Ⅱ type 2 receptor antagonist EMA401 on neuropathic pain in rats and its mechanism     

XIAO Han-yan  ZHANG Ben-zhuo  HAN Li-ping  XU Feng 《园艺学报》2017,33(1):110-115

AIM: To explore whether angiotensin Ⅱ type 2 receptor antagonist EMA401 decreases neuropathic pain and the expression of growth-associated protein-43 (GAP-43), protein kinase C (PKC) and calmodulin (CaM) in dorsal root ganglia (DRG) during chronic constriction injury (CCI) in rats. METHODS: SD rats were used to establish CCI model and randomly divided into 4 groups. The rats in model group were given equal volume of normal saline by intragastric administration. The rats in low dose (LD) group were given 5 mg/kg EMA401 by intragastric administration. The rats in middle dose (MD) group were given 10 mg/kg EMA401 by intragastric administration. The rats high dose (HD) group were given 20 mg/kg EMA401 by intragastric administration. The rats in sham operation group received equal volume of normal saline by intragastric administration. Thermal withdrawal latency (TWL) and mechanical withdrawal threshold (MWT) were measured before operation and 7 d, 14 d and 28 d after CCI. After behavioral test, DRG of lumbar spinal was obtained from each group, and was used to determine Ca²⁺ concentration by o-cresolphthalein complexone microplating method, and the expression of GAP-43, PKC and CaM at mRNA and protein levels by Western blotting and RT-PCR. RESULTS: Compared with model group, EMA401 significantly increased the TWL and MWT (P<0.05). Meanwhile, EMA401 significantly reduced Ca²⁺ concentration and the expression of GAP-43, PKC and CaM at mRNA and protein levels in the DRG (P<0.05). CONCLUSION: EMA401 may attenuate neuropathic pain of CCI by inhibiting Ca²⁺ concentration and the expression of GAP-43, PKC and CaM.  相似文献