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1.
The release of ammonia nitrogen during composting of sewage sludge mixed with a lignocellulosic bulking agent leads to a reduction in the agronomic value of the final compost and to harmful effects on the environment. We propose adding a cheap salt FeCl3 which can be used without special precaution to reduce ammonia losses by decreasing pH conditions. An in-vessel co-composting experiment was conducted in a large reactor (100 L) in which FeCl3 was added to sludge mixed with a bulking agent (pine shavings and sawdust) and compared with a control mixture without FeCl3. Temperature, oxygen consumption and pH were monitored throughout the composting of both mixtures. The final balance of organic matter, organic and inorganic nitrogen permitted to conclude that the addition of FeCl3 reduced nitrogen loss (by a factor of 2.4 in relation to the control) and increased mineralisation of the organic nitrogen by 1.6.  相似文献   

2.
The occurrence of bovine spongiform encephalopathy (BSE) in Canada has resulted in the implementation of regulations to remove specified risk material (SRM) from the food chain. SRM includes the distal ileum of all cattle, and the skull, brain, trigeminal ganglia, eyes, palatine tonsils, and spinal cord and dorsal root ganglia of cattle ≥30 months of age. Composting may be a viable alternative to rendering for SRM disposal. In our study, two bulking agents, barley straw and wood shavings, were composted with beef manure along with SRM in passively aerated, laboratory-scale composters. Both composts heated rapidly, exceeding 55°C after 3 days with oxygen declining in the early composting stage with wood-shaving compost, but returning to near-original levels after 5 days. During composting the two matrices differed (P <0.05) only in water content, TC and bulk density. In the final compost, water content, TC and C/N ratio were higher (P < 0.05), while EC was lower (P < 0.05) in the wood shavings as compared to the straw compost. Approximately 50% of SRM was decomposed after 15 days of composting, with 30% of SRM being decomposed within the first 5 days. Phospholipid fatty acid (PLFA) profiles were used to characterize the microbial communities and showed that Gram positive bacteria were predominant in compost at day 5, a point that coincided with a rapid increase in temperature. Gram negative bacteria and anaerobes declined at day 5 but populations recovered by day 15. Fungi appeared to be suppressed as temperatures exceeded 55°C and did not appear to recover over the remainder of the composting period, with the exception of the straw compost at day 15. On day 5, Actinomycetes increased in the straw compost, but declined in the wood shavings compost, with this group increasing in both types of compost at day 15. Although temporal changes were evident, compost matrices or depth within the composter did not obviously influence microbial communities. Decomposition of SRM also did not differ between compost matrices or with depth in the composters. These results suggest that SRM decompose rapidly during composting and that both mesophilic and thermophilic microbial communities play a role in its decomposition.  相似文献   

3.
An experiment comparing the effectiveness of three different bulking agents in the composting of fish processing wastes was carried out using small-scale composters operated at ambient temperatures. Fish offal was mixed with each of peat moss, sawdust and wood shavings in a 5:1 ratio by weight. The mixed materials were left undisturbed in the composters for an initial 55 days. They were then mixed and reloaded into their respective composters for a further 95 days of composting. Temperatures were monitored throughout the experiment, while serial physical and chemical analyses of the compost materials were initiated after the mixing. Headspace-gas chromatography was also used to determine the levels of phytotoxic organic acids and phenol. All the compost piles reached thermophilic temperature levels (40–70°C). Due to insufficient pile mass, temperatures over 55°C were not sustained for a sufficient length of time to satisfy the EPA requirements for pathogen reduction. The temperature profiles suggest that ambient temperatures near the freezing point can significantly influence the temperature of compost piles with volumes of 63 liters or less. The results reaffirm the potential of composting as a viable option for utilizing fish processing wastes and the suitability of peat moss, sawdust, and wood shavings as bulking materials. The physical and chemical qualities of the 150-day old composts were comparable to other seafood compost products. Peat moss gave the best results in terms of nitrogen conservation, but is the most expensive option. Monitoring the concentrations of phytotoxic organic acids and phenol using headspace-gas chromatography proved to be a useful method for establishing compost maturity.  相似文献   

4.
A small-pile demonstration study was conducted to evaluate the possibility of composting cranberry leaves on commercial cranberry farms in Southeastern Massachusetts. The recipe ratios tested were: 20:0:0 (cranberry leaves only), 10:10:2, and 10:10:1 (leaves:horse manure and bedding:fish hydrolysate). Both mixed recipes generated thermophilic temperatures (between 43-66°C for at least 20 d), conditions known to destroy many plant pathogens and weed seeds. Analysis of leaf samples indicated that all three recipes were more acidic than most other types of compost (values below pH 6). Nitrogen levels were at or below the low-end (0.45-0.75% N) of the typical level seen with most compost (1% N). The final C:N ratios (22:1 to 38:1) were within the acceptable range for many uses. The mixed piles attained thermophilic temperatures for sufficient duration to prevent germination of the seven species of weeds tested. Leaf residue levels for eight common cranberry pesticides were also determined. The insecticide, chlorpyrifos, and the metabolite of the herbicide dichlobenil, BAM (2,6-dichlorobenzamide), were detected at levels below food tolerance and slightly above the food tolerance threshold, respectively. These levels were within a range that would allow the pursuit of commercial development of the compost since ingestion is not an anticipated end-product use. The data from this preliminary demonstration study would suggest that the composted product would be acceptable for use on-farm as well as for distribution to other off-farm sites, but additional replicated research studies are warranted.  相似文献   

5.
Olive-tree leaves (OL) were mixed with olive press cake (OPC) and extracted olive press cake (EPC) at 1:1 dw/dw ratios to prepare two composting mixtures (OL+OPC and OL+EPC). Both CO2–C evolution and fluorescein diacetate (FDA) hydrolysis, determined as estimates of the microbial activity during composting, were related to temperature fluctuations in the compost piles, showing greater values at the temperature peaks, compared to the end, of each thermophilic phase. This, however, was only shown after handling and incubating samples at the temperatures of the compost mixtures at the sampling times and not at a low standard temperature. Incubating samples from thermophilic phases at low standard temperatures resulted in underestimation of the microbial activity occurring during composting. The effect of incubation temperature was less dramatic for FDA hydrolysis compared to CO2–C evolution measurements, probably reflecting the reduced dependence of enzymes involved in FDA hydrolysis on the respective temperatures. However, FDA hydrolysis was a less sensitive indicator of microbial activity, probably due to extracellular cleavage of fluorescein by persistent esterases, at lowered microbial activity phases. Total microbial biomass, estimated by the fumigation–extraction method, was not consistently related to temperature fluctuations during composting and showed a clear increase at the end of composting, probably resulting from a large slow-growing mycelial community colonising the end products. Since high temperatures did not induce significant non-microbial CO2–C release and FDA degradation, we propose the performance of microbial activity measurements during thermophilic composting phases at the actual temperatures evolving in the composts.  相似文献   

6.
Quantity and quality of readily degradable carbon influences the composting process especially for compost mixture high in lignocellulotic material. Effects of carbon source on stability and maturity of compost from in-vessel systems are poorly understood. Research was conducted to investigate the effects of carbon composition of feedstock on the evolution of stability indices and reliability of maturity tests for accelerated vessel composting systems. Rice straw, sugarcane bagasse, and coffee hulls were composted in a modified rotary in-vessel composter amended with either cattle or sheep manure. Distinct evolution patterns were observed across carbon sources for temperature, with the sugarcane compost never attaining thermophilic temperatures. Time to peak temperature and return to ambient were significantly different between the rice and coffee compost. Comparatively, organic matter degradation followed a similar pattern for all carbon sources, although rice straw showed the faster degradative rate and coffee hulls the greatest overall loss. Both pH and electrical conductivity were inappropriate stability indices across carbon sources, while the NH4+/NO3? ratio was lower than the threshold from week 1. The Solvita® maturity test was the best suited quality indicator and was related to compost respiration. The rice compost at week 12 was the only mature compost with an index value of 7. However, the coffee compost was in the curing stage with a value of 6. In vitro phytotoxicity assays on hot pepper contrasted the Sovita® interpretation for rice compost, which showed the lowest germination index. All compost had a stimulatory effect on cucumber seeds. In vivo seeding assays corroborated in vitro results with rice compost showing the greatest negative effect, augmented at 100% compost inclusion. Carbon source significantly influenced compost stability and maturity indices, which suggests that greater attention should be directed to quality indices in relation to feedstock composition.  相似文献   

7.
Composting broiler litter (a mixture of manure, bedding material, and wasted feed) with commonly available high-C substrates may be a viable alternative to reduce current land disposal practices for litter. Broiler litter with wood shavings as a bedding material and broiler litter with peanut hulls as a bedding material were composted with wheat straw, peanut hulls, pine bark and paper mill sludge in 0.33 m3 batch reactors. Litters and C substrates were mixed to achieve C:N ratios of approximately 30:1. Dry weight, total N, total C, temperature, electrical conductivity and pH were determined at regular intervals. Maximum temperatures peaked near 70°C within 2.25 d after mixing peanut hulls with litter and within 2.58 d for pine bark and litter. Composts made from paper mill sludge approached 50°C within 3.71 d. Wheat straw composts never exceeded 40°C which could present potential health problems associated with pathogenic microorganisms. Mass loss and C:N ratio gradually declined and stabilized approximately 84 d after mixing. Mass loss averaged 73 percent for wheat straw compost, 33 percent for peanut hull composts, and 16 percent for the other mixes. Wheat straw compost C:N ratios stabilized near 14:1 and other mixes remained above 20:1, indicating N limited conditions for complete composting. Compost pH was 5.8 after 84 d from pine bark composted with wood shaving litter and was significantly lower than pH from paper mill sludge compost with an average pH of 6.9 but similar to all other compost mixes (pH 6.7). Electrical conductivity ranged from 0.35 S m?1 for paper mill sludge composted with wood shaving litter to 0.91 S m?1 from wheat straw composted with peanut hull litter. Composting temperature varied considerably among C sources and all required at least 72 d of curing to stabilize the C:N ratio. Composts made from wheat straw were most effective for waste reduction but temperatures were below the 50°C level generally considered necessary to kill pathogens.  相似文献   

8.
以油枯为基本原料,采用好氧堆肥方式进行堆肥试验,研究了添加4种不同微生物菌剂的条件下,油枯-稻壳-甘蔗渣堆肥体系中pH、C/N、水溶性NO3--N、水溶性NH4+-N中的动态变化规律及菌剂对高温堆肥腐熟进程的影响。结果表明,添加菌剂能有效缩短堆肥到达高温的时间,延长高温分解阶段,加快物料水溶性NH4+-N和C/N降低,pH和水溶性NO3--N含量升高,加快了油枯堆肥腐熟化进程。添加VT菌剂的堆肥处理相比其他在堆肥15d后最先进入高温分解阶段,高温持续时间为10d,提早5d腐熟,水溶性NO3--N含量从71.41mg.kg-1增加到887.4mg.kg-1,C/N的降低有效促进了NH4+-N向NO3--N的转化,加快了油枯堆肥化进程,有助于提高堆肥腐熟化程度,说明添加VT菌剂的堆肥腐熟效果相对显著。  相似文献   

9.
A crab processing waste/wood shaving mixture was composted in-vessel with four intermittent aeration strategies. The composting process was monitored to assess the efficacy of the aeration methods. Temperature, moisture content and volatile solids of the compost were monitored along with the interstitial oxygen concentration. There was no significant difference in temperature profiles between constant frequency aeration and temperature feedback controlled aeration methods. An increased frequency of aeration resulted in significantly more drying than the less frequent aeration using the same total air volume. Results indicated that air volume could be reduced after the first five days of composting, and the in-vessel composting retention time could be reduced to 15 to 17 days for odor and vermin/vector problems prevention.  相似文献   

10.
Disposal of crawfish processing residuals (hereinafter, referred to as crawfish residuals) poses a challenging problem to the rapidly expanding crawfish industry. Cocomposting is examined as a waste management alternative to landfill disposal. Four agricultural processing by-products were evaluated for use as bulking agents in composting crawfish residuals: wood chips, rice hulls, bagasse, and bark. Approximately 5 to 6.5 volumes of each bulking agents were mixed with one volume of crawfish residuals in 0.3-m3 composting reactors. Compost temperature was continuously monitored, and moisture content was maintained within a desirable range. Samples were collected twice weekly throughout the 50-d composting process. Use of bagasse as a bulking agent led to the largest reduction in volatile solids (27.6 percent), organic C (55.3 percent), particle size (64.7 percent), and compost volume (52.8 percent). Finished compost using bagasse contained the greatest concentration of N (18.4 g N/kg and 160 mg NH4-N/kg). Self-heating patterns and decomposition of crawfish residuals were satisfactory using all four bulking agents, and no odor, insect or other nuisance problems were detected. The finished products of all compost mixtures were suitable for use as mulch or reuse as bulking agents.  相似文献   

11.
超高温预处理装置及其促进鸡粪稻秸好氧堆肥腐熟效果   总被引:8,自引:3,他引:5  
为提高畜禽粪便堆肥效率和质量,设计了一种超高温预处理好氧堆肥工艺,并以鸡粪和稻秸为原料,进行了为期62 d的堆肥试验。设置了3个处理,即:高温好氧堆肥(CK);超高温(85℃)预处理4 h+高温好氧堆肥(HPC);超高温(85℃)预处理4 h+接种0.5%新鲜鸡粪+高温好氧堆肥(I-HPC)。监测了堆体的温度、含水率、pH值等参数的变化情况,并以C/N、可溶性有机碳(dissolved organic carbon,DOC)、铵态氮、硝态氮、腐殖化指数(humification index,HI)、种子发芽指数(germination index,GI)为指标评价了堆肥腐熟度和质量。结果表明,与CK相比,超高温预处理可以提高后续堆料升温速率和最高温度、延长高温期天数、缩短堆肥周期,I-HPC、HPC的最高温度比CK分别高出13.6、12.8℃,≥50℃的天数分别比CK多3、2 d。但与HPC相比,接种新鲜鸡粪并没有加快后续堆肥进程。超高温预处理后,物料容重由0.81 g/cm~3下降为0.72 g/cm~3、pH值下降了1~2;而DOC质量分数由106 g/kg上升到124 g/kg;总挥发性脂肪酸(total volatile fatty acids,TVFAs)、NH_4~+-N质量分数分别为预处理前的3.2倍、2.45倍。HPC、I-HPC堆体有机质降解速率常数分别为0.0501、0.0534 d-1,比CK(0.00143 d-1)大,因此,HPC、I-HPC堆肥产品的TOC质量分数(182.1、192.1 g/kg)分别比CK(205.3 g/kg)低;TN质量分数(19.70、21.28 g/kg)比CK(17.96 g/kg)高;腐殖化指数(0.77、0.71)比CK(0.64)高。但HPC、I-HPC堆肥产品之间TN质量分数、腐殖化指数无显著差异。因此,超高温预处理好氧堆肥法能明显缩短堆肥周期、提高堆肥产品质量,具有较大的应用潜力。  相似文献   

12.
过磷酸钙用量对猪粪堆肥过程及磷形态变化的影响   总被引:6,自引:5,他引:1  
【目的】过磷酸钙作为常用的畜禽粪便堆肥添加剂,具有减少氮素损失率并提高氮、磷养分含量等优点,但其对磷的有效性和形态的影响有待深入探讨。通过试验研究不同过磷酸钙添加量对猪粪锯末好氧堆肥过程的影响及堆肥中不同形态磷含量和形态之间的相互转化规律,以期为畜禽粪便堆肥中磷素的转化及合理施用提供科学依据。【方法】将猪粪和锯末以质量比(鲜重)4:1的比例混合,然后按照猪粪和锯末干物质量的5%、10%、15%添加过磷酸钙,以不添加过磷酸钙作为对照(CK),进行42 d好氧堆肥,监测堆肥温度、含水率、pH、全氮、有机碳、全磷、有效磷、有机磷等指标。【结果】与CK相比,添加适量过磷酸钙可以显著提高堆肥的温度,增加高温持续期2~10 d;提高堆肥物料的持水能力,加快有机碳降解。添加5%~15%的过磷酸钙可以显著降低堆肥初始pH值0.33~0.68个单位,有效抑制堆肥过程中铵态氮的挥发损失,CK、5%、10%和15%的处理在堆肥结束时的氮素损失率分别为29.4%、26.6%、18.5%和8.0%,全氮和全磷含量分别较堆肥初增加17.3~34.2%和37.0%~123.1%。CK有效磷含量随堆肥时间不断上升,第42 d较堆肥初增加73.0%,而添加过磷酸钙5%、10%和15%的处理有效磷含量显著降低,分别较堆肥初减少了4.0%、23.2%和41.8%。随着堆肥的进行,各处理中有效磷占全磷的比例均不断下降,表明堆肥过程降低了磷的有效性。堆肥前期以有机磷的矿化为主,后期以有机磷的合成为主,第42 d有机磷占全磷的比例CK最高(22.1%),添加5%、10%和15%过磷酸钙的处理分别为15.4%、11.0%和8.7%。【结论】猪粪堆肥中添加过磷酸钙,可以有效减少氮素损失,加快有机物料降解,以10%的添加量效果最佳;堆肥过程降低了磷的有效性,随着过磷酸钙用量的增加有效磷的比例不断下降;腐熟后堆肥中的磷以无机态为主,有机磷占全磷的比例随着过磷酸钙用量的增加而递减。  相似文献   

13.
Cocomposting of poultry litter with municipal solid waste compost (MSW) was evaluated as a means to stabilize nitrogen and phosphorus in poultry litter and to produce a stable organic soil amendment. Four passively aerated compost piles were established by mixing fixed weight ratios of MSW and composted poultry litter (21:1, 6:1, 3:1, 1:1); moisture was adjusted to 50 percent by weight at pile establishment. These ratios represented a range of initial C:N (26-12) and C:P (150-50) ratios. Composting process parameters monitored over eight months included temperature, oxygen and moisture contents, pH, electrical conductivity, C:N:P ratios, microbial respiration and diversity. Initial feedstock ratios had no significant effect on temperature in the thermophilic phase of composting. After one year of composting, microbial respiration in 21:1 and 6:1 mixtures was high relative to 3:1 and 1:1 mixtures suggesting slow maturation in piles with high MSW content. Salmonella sp. and coliform organisms were detectable for up to 47 days. Results suggest that MSW has potential as a carbon feedstock for poultry litter composting when used in moderate amounts.  相似文献   

14.
Olive production is increasing very fast in the last 20 years in Al-Jouf region, northern region of the Kingdom of Saudi Arabia. There are now more than 5 million olive trees in the productive stage. A huge amount of waste is produced annually from this sector, including pruning waste of olive trees and solid waste from olive oil mills of three-phase system. The waste of olive used to produce compost could be of safe disposal technique. Disposal of such waste is expensive and it causes some environmental hazards. Rock phosphate (RP), which is available in the same region, can be used to improve the nutrient value of the produced compost. In this experiment olive pruning waste was composted alone and in a mixture with different RP in two different ratios, namely 5% and 10%, on the dry weight basis of olive waste. Compost processing took place on plies for 8 months. Temperature and moisture were monitored daily while periodical samples were taken from all piles for testing pH, EC, C/N, and germination index. Composts in all treatments reached the theomorphic phase, which killed weed seeds and pathogens. The produced compost from all treatments fulfilled the requirements of maturity according to the California Compost Quality Council after 8 months of composting. In addition, the RP enhanced composting efficiency and improved the quality of the produced compost. Compost parameters met the standard requirements for the compost that was suitable for agriculture purposes with average values of pH ranging from (7.02–7.65) and EC (2.20–3.94 dS m?1). Nutrient concentrations in the produced compost were N (1.28–1.79%), P (0.23–2.15%), and K (2.59–4.22%).  相似文献   

15.
接种高温嗜热菌剂加快牛粪秸秆堆肥发酵进程   总被引:1,自引:1,他引:0  
  【目的】  探究添加高温嗜热菌剂对牛粪堆肥的发酵效率、木质纤维素降解和堆肥品质的影响。  【方法】  以牛粪和玉米秸秆为原料进行堆肥。添加嗜热菌剂处理(GLL)的菌剂主要由普通高温放线菌(Thermoactinomyces vulgaris)、地尿素芽孢杆菌(Ureibacillus terrenus)和嗜热脱氮芽孢杆菌(Geobacillus thermodenitrificans)组成,以分别添加两个市售有机肥发酵菌剂的处理(A、B)为对照,同时设不接种菌剂的空白对照(CK),发酵试验为期30天。在堆肥第0、3、7、12、16、23、30天取样,烘干样用于测定堆肥木质纤维素含量,鲜样用于测定含水率、pH、电导率(EC)值、种子发芽率指数(GI)和细菌群落结构。  【结果】  GLL处理在堆肥第2天迅速升温至超高温期(85.8℃),超高温期持续5天;CK、A和B处理在堆肥第3天内进入高温期(分别为56.3℃、59.2℃和57.6℃),高温期分别持续了10、11和13天。接种GLL显著降低了堆肥水分含量,堆肥结束时含水量下降至34.3%,而CK、A和B处理的含水量分别下降至45.4%、43.8%和44.6%,未能满足产品水分标准。GLL处理半纤维素、纤维素和木质素在堆肥后比初始值分别下降81.6%、65.2%和53.7%,对木质纤维素的降解能力明显高于CK、A和B处理。在GLL处理堆肥高温期及超高温期,厚壁菌门细菌相对丰度增加到46.6%。接种GLL菌剂发酵后的堆肥产物的有机质、总养分、机械杂质质量分数及酸碱度、GI值等均满足NY/T 525—2021的要求。  【结论】  接种高温嗜热菌剂能够显著提高堆肥温度,延长高温期持续时间,实现超高温堆肥,降低水分含量,提高木质纤维素的降解效果,快速获得满足NY/T 525—2021要求的堆肥产品。  相似文献   

16.
  【目的】  掌握猪粪堆肥过程中微生物群落的演替规律与理化指标的相互关系,对提高猪粪堆肥营养品质和加速堆肥进程具有重要的意义。  【方法】  以猪粪和玉米秸秆 (质量比6∶1) 混合物为堆肥原料,耐高温菌剂主要含Acinetobacter pittii、Bacillus subtilis subsp. Stercoris和Bacillus altitudinis。堆体设接种菌剂 (MI) 和未接种 (对照,CK) 两个处理。常规监测堆肥温度和理化指标值,于堆肥开始后第4、12、24和32天采集样品,以16S rRNA高通量测序技术研究堆肥细菌群落的变化。用堆肥第4、12、24和32天采集的新鲜样品制备浸提液,进行紫花苜蓿种子发芽试验。堆肥结束时,测定全氮、全磷和全钾含量,并探讨微生物菌剂对堆肥理化指标和细菌群落演替的影响。  【结果】  接种微生物菌剂可使堆肥高温期提前2天出现,并且能延长高温期2天。堆肥浸提液的促生试验发现,在堆肥24天后紫花苜蓿种子发芽指数 (GI) 大于80%,且MI对幼苗主根的促生能力大于CK (P < 0.05)。随着发酵过程的进行,堆体体积不断缩小,CK和MI中全钾 (TK) 和全磷 (TP) 含量一直呈增加趋势,在堆肥第32天,CK和MI的全磷含量分别为2.28%和2.63%,处理间差异显著 (P < 0.05),而全钾含量分别为1.81%和1.86%,全氮含量分别为2.65%和2.63%,pH分别为8.72和8.78,处理间差异均不显著。在整个堆肥过程中,MI和CK的pH变化范围分别为8.40~8.95和8.61~8.93;CK和MI中总有机碳 (TOC) 的降解速率在堆肥的4~12和24~32天均表现为MI大于CK,CK和MI的碳氮比 (C/N) 分别为13.28和15.26,差异显著 (P < 0.05)。堆肥过程中在门水平上占据主导地位的细菌群落主要包括 Proteobacteria、Actinobacteria、Firmicutes和Bacteroidetes,在堆肥的高温期 (堆肥24天),堆体CK和MI中Firmicutes的相对丰富度分别为17.4%和59.8%;在堆肥的升温期、高温期和腐熟期,优势门水平细菌群落Proteobacteria、Firmicutes和Actinobacteria依次演替,且MI堆体中细菌群落的相对丰度均大于CK。属水平优势细菌为Acinetobacter、Lysinibacillus、Solibacillus、Pseudomonas和Flavobacterium,添加微生物菌剂可使Acinetobacter的丰度在堆肥第4天增加21.41%,此外,添加复合微生物可使堆肥第12天的Shannon和Observed species指数增加。相关性分析表明,温度、全N (TN)、TP、TK、TOC及GI与堆体中细菌组成具有显著相关性,而pH和细菌的相关性不显著。  【结论】  在堆体内接种微生物菌剂可显著提高并维持堆肥过程中优势门、属细菌群落的丰度,进而促进堆体升温并延长高温时期,缩短堆肥腐熟周期,加快总有机碳的分解,最终提高堆肥产品中有效磷的含量。复合微生物菌剂在堆肥升温期起主要作用的为Acinetobacter pittii,高温期为Bacillus subtilis subsp. Stercoris和Bacillus altitudinis,我们建议筛选耐高温细菌时应集中在厚壁菌门的芽孢杆菌属。  相似文献   

17.
  【目的】  研究嗜热复合菌对畜禽粪污堆肥理化特性和腐熟度的影响,探讨嗜热菌影响堆肥过程的微生物机制。  【方法】  堆料由75%羊粪和25%养鸡发酵床垫料构成,初始原料C/N为28,堆料量1.2 t,高度70~90 cm,开放条垛式堆沤。处理组为堆肥添加0.1%嗜热菌B. fordii FJAT-51578和U. thermosphaericus FJAT-51579等比混合的发酵液,对照组为添加1%市售枯草芽孢杆菌堆肥菌剂(Bacillus subtilis)。堆肥时间为2021年9月18日—10月14日,每两天检测1次温度。堆肥前15天,每两天进行一次翻抛,后期每5天进行一次翻抛,保持堆肥含水量50%~60%,直至高温期结束。在堆肥开始后第1、9和26天取堆肥样品,分析氮磷含量、硝化指数和种子发芽指数。结合扩增子测序,分析堆肥细菌群落结构变化,并揭示其主要环境影响因子。采用PICRUSt分析堆肥有效氮和有效磷代谢的微生物机制。  【结果】  嗜热复合菌添加促进堆肥硝化指数的降低和种子发芽指数的升高,促进堆肥腐熟;堆肥产物碱解氮和有效磷的含量分别比市售菌剂组高11.8%和7.7%。同时,嗜热复合菌的添加改变了细菌群落的分布,降低了堆肥细菌的多样性和丰富度,提高了糖单胞菌、链霉菌和嗜热葡萄孢菌等降解菌的丰度。RDA分析表明,pH和C/N是影响堆肥微生物群落多样性的主要因素,碱解氮与芽孢杆菌和糖单胞菌属丰度正相关,有效磷与嗜热裂孢菌、直丝菌属和马杜拉放线菌属丰度正相关。氮、磷代谢相关京都基因和基因组百科全书同源基因(KO)的PICRUSt分析显示,微生物氮磷循环相关KO的丰度随着堆肥进程均有所增加。添加嗜热菌剂提高了氨化、铵同化、硝酸盐同化、同化/异化硝酸盐还原等氮循环相关KO,及无机磷溶解、酸性磷酸酶和碱性磷酸酶等磷循环相关KO。  【结论】  在畜禽粪污堆肥中添加嗜热复合菌剂加快并延长了高温期,降低了C/N,提高了堆肥中碱解氮和有效磷含量,其中C/N、硝化指数和GI指数等指标在堆肥中期达腐熟程度标准,促进堆肥腐熟。堆肥中添加嗜热复合菌剂增加了细菌氮磷代谢相关KO的表达,提高了腐熟中期堆肥中嗜热菌的丰度和种类,碱解氮与芽孢杆菌和糖单胞菌属丰度呈正相关,有效磷与嗜热裂孢菌、直丝菌属和马杜拉放线菌属丰度正相关。因此,添加嗜热复合菌促进了堆肥有效氮磷的含量。  相似文献   

18.
In comparison to traditional windrow composting, in-vessel composting techniques often represent more effective waste management options due to the reduced production of bioaerosols and leachate and the potential for better process control. Chemical processes occurring during the cocomposting of three common wastes (green waste, biosolids and paper processing waste) were studied using the forced aeration, static pile, in-vessel EcoPOD® composting system. Since no turning of the compost occurs within the static piles, spatial differences in the vessel were also monitored. These measurements revealed significant spatial gradients in temperature; however, this did not result in spatial differences in nutrients within the composting vessel. Significant differences in soluble N production were observed during the composting process following the series: green plus paper waste < green waste < green waste plus biosolids. After the active compost phase was over, and the compost was removed from the vessel and matured outside, we demonstrated that covering the compost was essential to preserve compost quality. Our study clearly shows that cocomposting of common waste feedstocks can be used to successfully manipulate the chemistry of the final compost making it suitable for multiple end uses. In addition, our study demonstrated that careful management of the compost maturing phase is also required to maximise quality and minimize pollution.  相似文献   

19.
针对奶牛养殖场粪便含水率高,堆肥处理成本高的特点,采用以干燥玉米秸秆为调理剂,在较高初始含水率条件下(70%~80%),进行了强制通风堆肥槽和翻转式堆肥仓的对比试验,并且探讨了晾晒脱水作为预处理对堆肥效果的影响。结果表明,各处理堆体升温迅速,且均在50℃以上维持8~12d,满足堆肥无害化的卫生标准(GB7959—1987)要求。至堆肥结束时,各处理含水率均降至40%以下,C/N均降至20以下,WSOC均低于16g·kg-1,NH4+-N含量均低于0.4g·kg-1;除采用堆肥槽在初始含水率为65%下堆肥NH4+-N/NO3--N〉3尚未腐熟完全外,其他处理NH4+-N/NO3--N均小于0.5,腐熟情况较好;所有处理的GI均大于50%,其中采用堆肥槽在较高初始含水率堆肥和晾晒预处理后堆肥GI已达80%,基本消除了植物毒性。采用较为开放的堆肥槽时,以玉米秸秆作调理剂,在较高的初始含水率条件下堆肥效果更好;以晾晒脱水作为预处理后堆肥,可减少所需调理剂的用量,节约了堆肥的成本。  相似文献   

20.
堆肥反应器中硫磺对牛粪好氧堆肥的保氮效果研究   总被引:6,自引:0,他引:6  
利用堆肥反应器严格控制堆肥条件,以牛粪和蘑菇渣为原料进行好氧堆肥,在堆肥过程中添加硫磺粉,研究硫磺对堆肥温度、pH、氮素转化、硫素转化和保氮效果的影响。结果表明,堆肥中添加0.5%硫磺粉(T1)对堆肥温度没有显著影响,高温期(≥50℃)维持5.5 d; 而添加1.0%硫磺粉(T2)能快速升温,但堆肥高温期维持时间4.6 d。添加不同量的硫磺粉均能显著降低堆肥pH,与CK比较差异显著。添加硫磺粉能大幅度增加铵态氮含量,至堆肥结束时,T1和T2处理铵态氮含量分别是CK处理的15倍和24倍,差异极显著; 还增加了堆肥有效硫含量,至堆肥结束T1和T2有效硫含量分别较堆肥初始增加35.7%和77.1%。整个堆肥过程总氮(TN)含量呈增加的趋势,堆肥结束时CK、T1和T2处理的TN含量分别达15.8、16.5和16.9 g/kg,T1和T2分别比CK处理增加4.4%和7.0%。说明在牛粪好氧堆肥中添加0.5%或1.0%硫磺粉,均可起到一定的保氮作用,可大幅度提高堆肥铵态氮和有效硫的含量,改善了堆肥养分品质; 而且添加1.0%硫磺粉效果好于0.5%硫磺粉。但是添加1.0%硫磺粉缩短了堆肥高温期,降低了种子发芽指数,不利于堆肥的无害化进程。  相似文献   

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