共查询到17条相似文献，搜索用时 281 毫秒
1.
[目的]拟从分子水平对菜用枸杞进行鉴定。[方法]利用nrDNA ITS(核糖体DNA基因内转录间隔区)碱基序列测定的方法对5份菜用枸杞资源进行碱基序列测定并分析序列差异。[结果]首次获得5种菜用枸杞nrDNA ITS区碱基序列,整个ITS序列长度变异范围为628~632 bp,平均为630 bp,共有79个变异位点,占12.5%,保守位点553,占87.5%。基于nrDNA ITS序列差异的品种聚结果与依据形态指标的是划分结果相符。[结论]nrDNA ITS区测序分析可作为分子水平鉴定菜用枸杞不同种质来源的又一途径。 相似文献
2.
基于nrDNA ITS序列的18份宁夏枸杞资源的遗传多样性 总被引：7，自引：0，他引：7
[目的]利用nrDNA ITS序列,探讨18份宁夏枸杞资源的遗传多样性。[方法]采用改进CTAB法提取枸杞叶片基因组DNA,利用合成的特异引物对其DNA中nrDNA ITS区进行扩增、克隆,对目的片段测序分析并对测序结果进行聚类分析。[结果]通过测序首次获得了18种宁夏枸杞nrDNA ITS区碱基序列,整个ITS序列长度变异范围为559~634 bp,平均为612 bp,整个转录间隔区(ITS1+ITS2)对位排列后总长度为480 bp,包括194个变异位点,占40.4%;286个保守位点,占59.6%。聚类结果表明了18份宁夏枸杞的亲缘关系与差异,并将其分为3个大类。[结论]基于nrDNA ITS区序列分析在研究枸杞种质遗传多样性方面具有一定的优越性。 相似文献
3.
采用改进CTAB法提取枸杞(Lycium Linn)叶片DNA,利用合成的特异引物对其DNA中nr DNA ITS区进行扩增、克隆,对目的片段进行测序分析,以期利用ITS条形码序列对枸杞杂交种进行早期鉴定。结果表明,7份枸杞属不同种间杂交种的ITS序列长度变异范围为558~632 bp,平均为610 bp。排序后的总长度为632 bp,整个转录间隔区(ITS1+ITS2)对位排列后总长度为478 bp,共有138个变异位点,ITS1和ITS2分别为85和53个,占28.9%;保守位点340个,占71.1%;有6个信息位点,占1.3%;51个转换位点,12个颠换位点,其中ITS1区的信息位点所占比例低于ITS2区,而ITS1区的转换位点与颠换位点比值高于ITS2区。通过对宁夏枸杞、北方枸杞、黑果枸杞及其种间杂交育种产生的杂交后代分析,得出基于ITS聚类分析能够初步判别杂交后代与父母本的亲缘关系与差异,可以作为早期鉴定枸杞属不同种间杂交种后代的方法之一。 相似文献
4.
采用改进CTAB法提取枸杞(Lycium Chinense Mill.)叶片DNA,利用合成的特异引物对其DNA中nr DNA ITS区进行扩增,利用ITS条形码序列,对枸杞属种质资源进行鉴定,分析其亲缘关系。结果表明,测序得到了17份枸杞属近缘种的ITS条形码序列,整个ITS序列长度变异范围为603~632 bp,平均为624 bp,整个转录间隔区(ITS1+ITS2)对位排列后总长度为480 bp,有194个变异位点,占40%;保守位点288个,占60%。聚类分析结果表明,17份种质资源可分为5个大类群。基于ITS条形码序列分析在鉴定枸杞属种质遗传多样性及其亲缘关系具有一定的优越性。 相似文献
5.
6.
采用PCR直接测序法,测定23种兜兰属植物的内部转录间隔区(ITS)序列,并结合GenBank中所查到的德氏兜兰(AJ564372)、波瓣兜兰(AY530916)及麻栗坡兜兰(AJ564357)的核糖体DNA(nrDNA)ITS区序列进行比对,确定23种兜兰的ITS1、5.8S及ITS2的界限。结果表明:23种兜兰植物的nrDNA ITS序列长度为717～787 bp;G+C含量为48.6%～51.5%,其中ITS1和ITS2长度分别为393～451 bp及155～180 bp,包括211个变异位点;109个信息位点。基于贝叶斯法和最大简约法构建系统聚类树,结果表明,2种方法构建的树基本一致,整个树分为2个分支3个亚组,表明基于nrDNA ITS序列能够鉴定分析兜兰属植物种间的亲缘关系。 相似文献
7.
《（《农业科学与技术》）编辑部》2008,(1)
ITS区序列指DNA基因内的转录间隔区序列,包括ITS1、ITS2及5.8S3个区段。ITS区序列测定分析能够鉴别同属植物相似种以及药用植物混淆种。笔者以枸杞属内不同种间ITS序列为切入点,对枸杞nrDNA ITS序列进行分析研究,为从分子水平鉴定枸杞品种提供参考。1材料与方法1.1试验材料供试 相似文献
8.
9.
对紫薇属植物细胞核核糖体DNA转录间隔区（nrDNA ITS）序列进行分析,为紫薇植物的鉴定提供分子依据。通过提取紫薇幼苗总DNA,对nrDNA ITS序列进行PCR扩增测序,应用软件BioEdit、DNAMAN 进行序列分析, MEGA计算遗传距离,构建基于K2P模型的NJ系统发育树。测序得到2条nrDNA ITS序列,长度均为615 bp。序列分析结果显示,序列1与紫薇ITS序列相似性达99．03％,遗传距离为0．003,聚类分析归为一支;序列2与毛紫薇ITS序列相似性达98．55％,遗传距离0．007,聚类分析归为一支。试验结果说明,基于nrDNA ITS序列分析法,能够对紫薇属植物进行准确的分子鉴定,从而为紫薇属的种类鉴定和种间亲缘关系提供分子生物学理论依据。 相似文献
10.
《（《农业科学与技术》）编辑部》2008,(2)
笔者以5种菜用枸杞为试验材料,开展nrDNA ITS序列分析研究,探索适宜于枸杞nrDNAITS序列分析的优化技术体系,从而为建立分子水平的菜用枸杞鉴定标准提供参考。1材料与方法1.1材料宁夏农林科学院枸杞中心枸杞种质资源圃内的5个菜用枸杞种质:宁夏枸杞(宁杞1号小叶枸杞)、宁杞菜1号 相似文献
11.
12.
Botanical features of "YX-1" reproductive organ and bearing rate by number of pollen granules in pollen sacs of Lycium chinensis was observed and researched through field investigation, smear preparation and paraffin sections. YX-1 of Lycium chinensis was determined as incomplete male sterility and further classified into complete sterile type, sterile type I and sterile type II. The fertility standard of male sterility of Lycium chinensis was preliminarily proposed to establish a basis for crossbreeding of male sterility of Lycium chinensis. 相似文献
13.
基于nrDNA ITS序列的18份宁夏枸杞资源的遗传多样性(英文) 总被引：3，自引：1，他引：2
[Objective] The study aimed to investigate the genetic polymorphism of eighteen Lycium barbarum resources via nrDNA ITS sequencing. [Method] The genomic DNAs from Lycium barbarum leaves were isolated by modified CTAB method for PCR amplification on the nrDNA ITS region using specifically synthesized primers; the amplified fragments were cloned and sequenced, then the sequencing results were clustered. [Result] nrDNA ITS sequences of the tested eighteen Lycium barbarum were firstly obtained in the present study. For all eighteen tested materials, the variation range of whole ITS region was 559-634 bp, with an average of 612 bp; alignment analyses showed that the whole length of internal transcribed spacer (ITS1+ITS2) was 480 bp, within which there are 194 variation sites (accounting for 40.4%) and 286 conserved sites (accounting for 59.6%). The cluster results showed that the eighteen tested materials could be grouped into three classes. [Conclusion] Analysis of nrDNA ITS sequence may avail to identify the Lycium barbarum germplasm resources. 相似文献
14.
15.
SHI Zhi-gang AN Wei JIAO En-ning ZHAO Jian-hua WANG Ya-junNingxia Wolfberry Engineering Technology Research Center YinChuan 《（《农业科学与技术》）编辑部》2008,(2)
[Objective] The study aimed to identify Lycium Linn. at molecular level.[Method] The nrDNA ITS sequence of 5 edible Lycium Linn. germplasm resources were investigated. [Result] The nrDNA ITS regions of five edible Lycium Linn. germplasm resources were sequenced. The whole sequences varied from 628 bp to 632 bp,with the average length of 630 bp. Total 79 variation sites were observed in the sequences,which accounts for 12.5%. [Conclusion] Sequence analysis based on nrDNA sequencing provides a new approach to identify edible Lycium Linn. germplasm resources. 相似文献
16.
[ObJective] The study aimed to identify Lycium Linn. at molecular level. [Method] The nrDNA ITS sequence of 5 edible Lycium Linn. germ-plasm resources were investigated. [Result] The nrDNA ITS regions of five edible Lycium Linn. germplasm resources were sequenced. The whole se-quences varied from 628 bp to 632 bp, with the average length of 630 bp. Total 79 variation sites were observed in the sequences, which accounts for 12. 5%. [Conclusion] Sequence analysis based on nrDNA sequencing provides a new approach to identify edible Lycium Linn. germplasm resources. 相似文献