共查询到20条相似文献，搜索用时 31 毫秒
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AIM To observe the effect of tanshinone ⅡA on liver lipid deposition and ferroptosis-related protein expression in ApoE -/- mice. METHODS Thirty-two ApoE -/- mice were randomly divided into model group, high-dose (60 mg/kg) tanshinone ⅡA group, low-dose (30 mg/kg) tanshinone ⅡA group and simvastatin group, and C57BL/6J mice (n =8) were used as normal control group. The mice in normal control group were given the basic feeding, while the others were given high-fat diet. The mice in tanshinone ⅡA groups and simvastatin group were given corresponding drugs. The mice in normal control group and model group were intraperitoneally injected with equal volume of saline. Eight weeks later, the serum levels of triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) were tested by automatic biochemistry analyzer. The liver tissues were stained with HE and oil red O. The contents of reactive oxygen species (ROS) and glutathione (GSH) in liver tissues of the mice were measured by commercially available kits. The liver glutathione peroxidase 4 (GPX4) and p53 were detected by immunohistochemical method. The protein and mRNA expression levels of ferroptosis-related factors GPX4, xCT/SLC7A11, p53 and ferritin heavy chain 1 (FTH1) were determined by Wes automatic Western blot quantitative analysis system and RT-qPCR. RESULTS Compared with normal control group, the serum levels of TC, TG and LDL-C in model group were increased significantly (P <0.05 or P <0.01), and HDL-C did not change significantly. The fat vacuoles were clearly visible in liver tissue. The content of ROS in liver tissue was increased significantly,and GSH was decreased significantly (P <0.01). The mRNA and protein expression levels of p53 were increased significantly, and GPX4, xCT/SLC7A11 and FTH1 were decreased significantly (P <0.05 or P <0.01). Compared with model group, tanshinone ⅡA significantly decreased the serum levels of TC, TG and LDL-C (P <0.05 or P <0.01), and HDL-C did not change significantly. High-dose and low-dose tanshinoneⅡA also significantly decreased the degree of steatosis, and the size of lipid droplets. The content of ROS in liver tissues was decreased significantly, and GSH was increased significantly (P <0.01). The mRNA and protein expression levels of GPX4, xCT/SLC7A11 and FTH1 were increased significantly, and p53 were decreased significantly (P <0.05 or P <0.01). CONCLUSION Tanshinone ⅡA reduces liver lipid deposition and lipid peroxidation damage in ApoE -/- mice, which may be related to the intervention of ferroptosis-related proteins in the liver cells. 相似文献
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AIMTo investigate the effects of Yangjing-Zhongyu decoction on the in vitro maturation and expression of platelet-derived growth factor subunit A (PDGFA) and platelet-derived growth factor receptor α (PDGFRα) of mouse oocytes. METHODSThe SPF female KM mice were given Yangjing-Zhongyu decoction, and the blood was collected to prepare serum. The serum containing Yangjing-Zhongyu decoction was used to culture immature oocyte-granulosa cell complexes. After the in vitro culture, the oocyte maturation rate, fertilization rate and cleavage rate were observed and calculated, and the expression of PDGFA and PDGFRα in the oocytes at protein and mRNA levels was determined by West?ern blot and real-time PCR. RESULTSYangjing-Zhongyu decoction increased the in vitro maturation rate and fertil?ization rate of the oocytes (P <0.05 or P <0.01), and down-regulated the protein and mRNA expression of PDGFA and PDGFRα (P <0.01). CONCLUSION Yangjing-Zhongyu decoction may promote the in vitro maturation of mouse oocytes by down-regulating the expression of PDGFA and PDGFRα. 相似文献
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AIM: To observe the effects of arsenic trioxide (As2O3) on activities of matrix metalloproteinases (MMPs), expression of tissue inhibitor of metalloproteinase-1 (TIMP-1) and transforming growth factor beta1 (TGF-β1) in human fibroblast (hFb), and to discuss weather As2O3 promotes the healing of chronic skin ulcer through regulating collagen metabolism. METHODS: Zymography was used for testing activity of MMP-9 deriving from rat polymorphonuclear neutrophils (PMNs) and activities of MMP-1, MMP-2 secreted by hFb. Immunocytochemical method was used to determine the expressions of TIMP-1 and TGF-β1. RESULTS: At the concentration of 50 mg/L, As2O3 elevated the activity of MMP-9 (P<0.01). At the concentration of 0.8 mg/L, As2O3 increased the activities of MMP-1 and MMP-2 (P<0.01, respectively). After hFb was cultured with As2O3 for 6 h, 12 h and 18 h, the expressions of TIMP-1 and TGF-β1 decreased continuously (P<0.01). CONCLUSION: As2O3 elevates the activities of MMP-1, MMP -2 and MMP-9, also inhibits the expressions of TIMP-1 and TGF-β1, suggesting that arsenic preparation may exert positive effect on healing chronic skin ulcer through regulating collagen metabolism. 相似文献
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Seed dressing with fungicides adversely affects the structure and function of beneficial soil microbial communities and consequently crop yield. This study was aimed to evaluate the impact of technical-grade fungicide tebuconazole on plant growth promoting potentials of tebuconazole-tolerant Rhizobium isolate MRP1. The performance of the isolate MRP1-inoculated pea plants grown in tebuconazole treated soils was also assessed. Generally, the three concentrations [100 (recommended dose), 200 and 300 μg kg−1 soil] of tebuconazole when used alone, adversely affected the growth, symbiosis, grain yield and nutrient uptake by pea plants. Concentration dependent phytotoxicity of tebuconazole was observed for all the measured parameters. On the contrary, fungicide tolerant Rhizobium sp. MRP1 in the presence of fungicide increased the measured parameters at all tested concentrations. As an example, when inoculant MRP1 was also used with 300 μg tebuconazole kg−1 soil, it substantially increased the root nitrogen, shoot nitrogen, root P, shoot P, seed yield and grain protein by 20, 19, 50, 31, 15 and 7%, respectively, when compared with uninoculated plants grown in fungicide-treated soils. The study suggests that the plant growth promoting Rhizobium sp. MRP1 can be used as bacterial inoculant to increase the production of pea in soils polluted with fungicides. 相似文献
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Strawberry (Fragaria × ananassa Duch.) is rich source of dietary antioxidants, minerals and nutrients. Dietary antioxidants have been known as beneficial for enhancing the fitness, preventing certain diseases and even mitigating the effects of ageing. The objectives of the present study were to determine variability and inheritance of antioxidants, to identify antioxidant rich and productive genotypes, and to suggest suitable breeding approaches. The genotypes, namely Ofra, Chandler, Festival and Camarosa showed higher concentrations of dietary antioxidants and therefore could be useful in future breeding. Results indicate that the effect of the genotypes on antioxidant contents is stronger than that of the environment. The high heritability (>80%) and low genetic advance as percentage of mean (<40%) for ascorbic acid and β-carotene contents could be improved by heterosis breeding. However, selection and hybridization would be effective tools to enhance the phenols and anthocyanin content, and yield potential as these traits showed high heritability (>80%) and high genetic advance as percentage of mean (>40%). Positive direct effect on fruit yield was highest for phenol content (0.609) which is also fairly close to its correlation coefficient (0.765) indicating that a direct selection based on phenol content would be most effective and that the phenol content could be used as a reliable biochemical marker to identify the productive genotypes having higher amounts of dietary antioxidants. The information could also be used for developing antioxidant rich cultivars, i.e. ‘Breeding Strawberry for High Antioxidants’. 相似文献
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AIM To investigate the effect of ClC-3 chloride channel/antiporter knockdown in rat dorsal root ganglion (DRG) on voltage-gated sodium channel expression in neurons and mechanical allodynia in rats. METHODS Adeno-associated virus carrying ClC-3 shRNA (AAV-ClC-3 shRNA) was injected intrathecally to knock down ClC-3 expression in DRG tissues of adult SD rats. The mRNA and protein expression levels of ClC-3, cytokines and voltage-gated sodium channels were detected by RT-qPCR, immunofluorescence and Western blot. The mechanical sensitivity was assessed using von Frey hairs and up-down method. RESULTS Intrathecal injection of AAV-ClC-3 shRNA decreased ClC-3 expression in the DRG tissues and induced mechanical allodynia in the rats. Knockdown of ClC-3 up-regulated the expression levels of Nav1.3, Nav1.7, Nav1.8 and Nav1.9 in the DRG tissues. Knockdown of ClC-3 increased tumor necrosis factor-α (TNF-α) and decreased interleukin-10 (IL-10) levels in the DRG tissues. CONCLUSION Knockdown of ClC-3 in rat DRG tissues induces TNF-α/IL-10 imbalance and increases expression of voltage-gated sodium channels, thus contributing to mechanical allodynia. 相似文献
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AIM: To approach the changes of ET and NO levels of mice bearing sarcoma 180 (S180 ) during different period(5、10 and 15 days) and the relationship between ET、NO and tumor’s development METHODS: Adopting mice bearing S180 as the models, the levels of ET and NO2-/NO3- in serum were detected, and the weight of isolated tumors was measured On the basis of the regulation of these changes, their relationships were explored RESULTS: The levels of ET and NO2-/NO3- of mice bearing S180 were higher than that of the control group (not bearing tumor) ( P< 0.05) Along with the development of the tumors, the levels of NO2-/NO3-and tumors weight both increased ( P< 0 05) ET also had an increasing tendency There was a positive correlation between the level of NO2-/NO3- and tumor weight ( r =0.995, P< 0.05) Ratio value of (NO2-/NO3-)/ET decreased at first and then increased CONCLUSION: ET and NO have links with the development of S180 There may be cooperation between ET and NO during the development of tumor. 相似文献
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β-Asarone content in Acorus calamus is a paramount issue because it limits the usage of plant for medicinal purpose. In the present study A. calamus L. accessions based on RAPD marker, ploidy level and β-asarone content were characterized and correlated on the basis of β-asarone content/ploidy level. Of the 40 random primers used, 6 primers generated polymorphism. Genetic relatedness among accessions evaluated by a similarity matrix based on Dice's coefficient ranged from 0.72 to 0.97. A phenetic dendrogram based on UPGMA analysis grouped accessions into two clusters. A. calamus L. accessions were found to be triploid and tetraploid and their β-asarone content was found in two ranges 6.92–8.0% and 73–88%. The study clustered the accessions as per their ploidy level, β-asarone content and geographical locations. This study would have extensive application in quality control of raw materials. 相似文献
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MA Dong-xue GAO Wei-juan LIU Zhen-yi ZHANG Hong-jun WANG Jia-ying DONG Xian-hui 《园艺学报》2021,36(12):2198-2204
AIM To explore the effect of compound of Epimedium , Astragalus and Radix Puerariae on the expression of a disintegrin and metalloproteinase 10 （ADAM10） in Aβ-induced hippocampal neuron HT22 cells with or without hepcidin （HAMP） expression knock-down for analyzing the pathogenesis of Alzheimer disease （AD） at cell level. METHODS Hippocampal neuron HT22 cells were cultured in vitro and randomly divided into 7 groups： control group, Aβ group （Aβ25-35-induced HT22 cells）, RNAi group （HAMP gene was silenced in HT22 cells）, Aβ+RNAi group （HAMP gene expression in Aβ25-35-induced HT22 cells was silenced）, Aβ+TCM group （Aβ25-35-induced HT22 cells were treated with Epimedium , Astragalus root and Radix Puerariae effective components）, RNAi+TCM group （HT22 cells with HAMP gene silence were treated with Epimedium , Astragalus root and Radix Puerariae effective components） and Aβ+RNAi+TCM group （Aβ25-35-induced HT22 cells with HAMP gene silence were treated with Epimedium , Astragalus root and Radix Puerariae effective components）. The silence efficiency of HAMP siRNA was detected by qPCR and Western blot. The ADAM10 expression in each group was determined by immunofluorescence, qPCR and Western blot. RESULTS The HAMP siRNA-3 sequence had the highest interference efficiency. Compared with control group, the expression levels of ADAM10 in Aβ group, RNAi group and Aβ+RNAi group were decreased （P <0.05）. Compared with Aβ group,the expression levels of ADAM10 in Aβ+RNAi group was also decreased （P <0.05）, and the expression levels of ADAM10 in Aβ+TCM group was increased （P <0.05）. Compared with RNAi group, the expression levels of ADAM10 in Aβ+RNAi group was decreased （P <0.05）, while the expression levels of ADAM10 in RNAi+TCM group was increased （P <0.05）. Compared with Aβ+RNAi group, the expression levels of ADAM10 in Aβ+RNAi+TCM group was increased （P <0.05）. CONCLUSION The effective components of Epimedium , Astragalus and Radix Puerariae compound promotes the expression of ADAM10 in Aβ25-35-induced HT22 cells, which mechanism may be related to the expression of HAMP. 相似文献
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QU Yi-ming SHAO Gao-hai ZHANG Ming-hua LI Bo ZHU Feng-chen HE Chao CAO Chun-feng ZHAO Bo 《园艺学报》2020,36(6):1089-1095
AIM To explore the effect of platelet-rich plasma (PRP) on rabbit osteoarthritis and its possible mechanism. METHODS The rabbits with knee osteoarthritis were prepared and then divided into model group, sodium hyaluronate (SH) group and PRP group, and another sham operation group was set up, with 6 rabbits in each group. The gross morphological changes of rabbit cartilage were observed. HE staining was used to evaluate the pathomorphological changes of the cartilage. TUNEL staining was used to detect the apoptosis of chondrocytes. The expression of nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3)/interleukin-1β （IL-1β） signaling pathway-related molecules was observed by immunohistochemical staining, and the protein levels of caspase-3, Bcl-2 and Bax were determined by Western blot. Chondrocytes were isolated and processed according to grouping, and the NLRP3 and IL-1β levels of the cells were measured by ELISA. RESULTS Compared with sham operation group, Pelletier score, Mankin score, chondrocyte apoptotic rate, the positive protein expression rates of NLRP3, apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC), caspase-1 and IL-1β, and the protein levels of caspase-3 and Bax in model group were increased significantly (P <0.05), while the protein expression of Bcl-2 was decreased significantly (P <0.05). Compared with model group, Pelletier score, Mankin score, the apoptotic rate of chondrocytes, the positive protein expression rates of NLRP3, ASC, caspase-1 and IL-1β, and the protein levels of caspase-3 and Bax in SH group and PRP group were decreased significantly (P <0.05), while the protein expression of Bcl-2 was increased significantly (P <0.05). In PRP group, Pelletier score, Mankin score, the apoptotic rate of chondrocytes, the positive protein expression rates of NLRP3, ASC, caspase-1 and IL-1β, and the protein levels of caspase-3 and Bax were lower than those in SH group, while the protein expression of Bcl-2 was higher than that in SH group (P <0.05). Compared with control group, the expression of NL?RP3 and IL-1β in MCC950 (NLRP3 ihibitor) group were significantly reduced (P <0.05), the expression of NLRP3 in eucalyptol (IL-1β inhibitor) group was not significantly changed (P >0.05), and the expression of IL-1β was significantly reduced (P <0.05). CONCLUSION Platelet-rich plasma promotes the repair of cartilage in osteoarthritis rabbits, which has better effect than SH. The mechanism may be related to the inhibition of NLRP3/IL-1β pathway and the reduction of chondrocyte apoptosis. 相似文献
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AIM: To investigate whether human prorenin can active the (pro)renin receptor leading to the phosphorylation of extracellular regulated-kinase 1 and 2 (ERK1/2) and whether the putative (P)RR blocker "handle-region" peptide (HRP) can inhibit this pathway in cultured human renal mesangial cells (HRMCs). METHODS: HRMCs were cultured in vitro and were pretreated with AT1 blocker olmsartan and AT2 blocker PD123319 for 30 min, then they were stimulated by prorenin, PD98059 (inhibitor of ERK1/2) and HRP, respectively. Phosphorylated ERK1/2 was evaluated in Western blotting method. The concentration of transfer growth factor-β (TGF-β) was measured using ELISA method. The mRNA of TGF-β was evaluated by RT-PCR. RESULTS: We found that prorenin induces the activation of (P)RR in cultured HRMCs, in turn, increased the phosphorylated ERK1/2. The protein level of TGF-β was up-regulated by the stimulation of prorenin. ERK1/2 inhibitor PD98059 significantly decreased the phosphorylated ERK1/2 and then down-regulated the TGF-β. HRP could inhibit neither the phosphorylation of ERK1/2 nor the increase of TGF-β.CONCLUSION: Prorenin induces the phosphorylation of ERK1/2 in cultured HRMCs owing to the combination to (P)RR. The phosphorylation of ERK1/2 leads to TGF-β increasing dramatically. It probably plays a key role in the development of kidney disease. HRP affects neither the signaling of ERK1/2 nor the TGF-β. 相似文献
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AIM: To investigate the role of transforming growth factor β1 (TGF-β1)/Smads and extracellular signal-regulated kinase(ERK) expression in vascular remodeling induced by high-salt diet in Wistar rats. METHODS: Wistar rats were randomly divided into 3 groups: normal control group (n=13), high salt (8%) model group and high salt+telmisartan group (n=13). Tail-cuff arterial pressure was determined every 2 weeks. After 24 weeks, the rats in high salt model group were divided into model animals with hypertension group (MH, n=12) and model animals without hypertension group (MN, n=12). The remodeling of aorta and mesenteric artery was observed by HE and Masson staining. In addition, the techniques of immunohistochemistry and real-time PCR were applied to detect the expression of proliferating cell nuclear antigen (PCNA), TGF-β1, p-Smad2/3, p-ERK1/2 and Smad7 at both protein and mRNA levels. RESULTS: Compared with normal control group, blood pressure in MH group was much higher, and media thickness (MT) and collagen volume fraction (CVF) of arteries in MH and MN groups were higher.The mRNA expression of TGF-β1, Smad2 and Smad7 in the aorta was significantly increased, and the protein levels of PCNA, p-ERK1/2, TGF-β1 and p-Smad2/3 in the aorta and mesenteric artery media were elevated, but Smad7 decreased. After telmisartan treatment, MT and CVF were much lower,and the protein levels of PCNA, TGF-β1, p-Smad2/3 and p-ERK1/2 were significantly reduced, whereas Smad7 was increased. CONCLUSION: The abnormal expression of TGF-β1/Smads and ERK may be involved in the mechanism of remodeling of aorta and mesenteric artery induced by high-salt diet. Telmisartan prevents the vascular remodeling via regulating TGF-β1/Smads and ERK signal pathways mediated by angiotensinⅡ type 1 (AT1) receptor, at least in part. 相似文献
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AIM:The present study was to investigate the roles of protein kinase C α and δ isoforms (PKC-α, δ) in arginine vasopressin (AVP) improved contractile response of vascular smooth muscle cells (VSMC) to norepinephrine (NE) after hypoxia and its relations to myosin light chain (MLC20) phosphorylation, myosin light chain phosphatase (MLCP) and myosin light chain kinase (MLCK) activity.METHODS: Primary cultures of VSMC were obtained from the superior mesenteric artery (SMA) of rats by explanting technique and the cells in third to fifth passage were used in the study. The effects of PKC-α and δ antagonists on AVP induced contractile response of VSMC to NE after 1.5 h hypoxia were observed by measuring the ratio of accumulative infiltration of fluorescent isothiocyanate-conjugated bovine serum albumin with transwell, and their effect on the activity of MLCP/MLCK in VSMC was assayed by enzymatic catalysis. At the same time, with the SMA from hemorrhagic shock rats (30 mmHg for 2 h), the effects of PKC α and δ isoforms in the regulation of AVP on MLC20 phosphorylation of SMA after shock were observed by Western blotting.RESULTS: G 6976 (5×10-6 mol/L, PKC-α isoform inhibitor) significantly antagonized AVP (5×10-10 mol/L)-induced increase in the contractile response of VSMC to NE after hypoxia, and rottlerin (10-5 mol/L, PKC-δ isoform inhibitor) also partly inhibited this effect. Hypoxia resulted in a significant increase in MLCP activity, with a decrease in MLCK activity of VSMC, and at the same time, the MLC20 phosphorylation of SMA following hemorrhagic shock was significantly decreased. AVP inhibited the activity of MLCP and increased the phosphorylation of MLC20, which was inhibited by G 6976, while rottlerin treatment only showed a slightly inhibitory effect. AVP and PKC-α, δ inhibitor had no significant influence on MLCK activity.CONCLUSION:AVP up-regulates vascular reactivity and calcium sensitivity of VSMC possibly through inhibiting the activity of MLCP and increasing the phosphorylation of MLC20 by PKC-α isoform. 相似文献
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AIM: To investigate the effect of caveolin-1 and phosphorylation of ERK1/2 on 17β-estradiol (E2) induced inhibition of vascular smooth muscle cells (VSMCs). METHODS: The proliferation in cultured VSMCs was determined by using ［3H］-thymidine incorporation. The expressions of caveolin-1, MKP-1 and ERK1/2 phosphorylation were measured by Western blotting. The expression of caveolin-1 mRNA was measured by RT-PCR. RESULTS: Exposed to fetal calf serum (FCS) for 24 h, the increase in proliferation of VSMCs was detected by ［3H］-thymidine incorporation. Pretreatment with various concentrations of E2 for 24 h inhibited VSMC proliferation induced by FCS. The results of Western blotting and RT-PCR showed that pretreated with 17β-estradiol for 24 h reserved the decrease in caveolin-1 induced by FCS. Western blotting results further proved that the expression of MKP-1 was significantly increased and the expression of ERK1/2 phosphorylation was decreased after incubated with 17β-estradiol. CONCLUSION: 17β-estradiol increases caveolin-1 and MKP-1 expressions, and decreases ERK1/2 phosphorylation, leading to the inhibition of VSMC proliferation. 相似文献
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