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为探讨奶牛子宫内膜炎和隐性乳房炎之间的相关性,在宁夏地区3个大型奶牛场(根据临床型子宫内膜炎的诊断标准)选择子宫内膜炎患牛111头,用兰州隐性乳房炎检测法检测(LMT)乳样,其中82.5%的病牛同时患有隐性乳房炎,然后进行了子宫样和乳样的采集,并用常规细菌分离、培养结合高智能全自动细菌鉴定及药敏分析系统(VITEK 2 Campact),确定引起宁夏奶牛子宫内膜炎的病原菌有18种110株,引起乳房炎的病原菌有23种107株,其中大肠杆菌、停乳链球菌、克氏库克菌、金黄色葡萄球菌、假单胞杆菌、乳房链球菌、产色葡萄球菌、蜡样芽孢杆菌、荧光假单胞杆菌、产酸克雷伯菌、副溶血弧菌、克雷伯氏菌可以同时引起奶牛子宫内膜炎和隐性乳房炎。 相似文献
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《中国兽医学报》2016,(4)
为给黄土高原舍饲养殖场科学防治奶牛子宫内膜炎提供理论依据,对该地区2市4县13个规模奶牛场和5个自然村,共77头患子宫内膜炎的奶牛子宫分泌物进行细菌分离鉴定,并对致病性不明的主要分离菌进行了大鼠子宫致病性试验。结果分离鉴定出12个菌属、23个菌种、108株细菌,主要细菌为芽孢杆菌(30.56%)、大肠埃希菌(14.81%)、肠球菌(12.96%)、葡萄球菌(9.26%)、乏养球菌(9.26%)、链球菌(6.48%)等;对主要分离菌的致病性研究发现除缺陷乏养球菌外,凝结芽孢杆菌、大肠杆菌(O20)、屎肠球菌等均可引起子宫内膜炎。表明引起该地区奶牛子宫内膜炎的致病菌主要为金黄色葡萄球菌、表皮葡萄球菌、溶血葡萄球菌、化脓链球菌、不动杆菌、凝结芽孢杆菌、大肠杆菌(O20)、屎肠球菌,可为有效治疗本地区奶牛子宫内膜炎提供可靠的理论基础。 相似文献
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对某奶牛场30头奶牛子宫内膜炎致病菌的分离鉴定结果表明,主要致病菌是大肠杆菌、链球菌和葡萄球菌。采集经产健康奶牛阴道黏液,经细菌分离纯化以及形态和生化鉴定,结果表明,主要的细菌是嗜酸乳杆菌、表皮葡萄球菌、柠檬酸肠杆菌、大肠埃希氏杆菌、多粒芽孢杆菌、肠膜明串珠菌、短小芽胞杆菌、干燥奈瑟氏球菌、金黄色葡萄球菌。用经产健康奶牛阴道正常细菌培养的全菌液和过滤液,对三种致病菌进行拮抗试验,参照抗生素药敏试验标准判定结果。结果表明,在上述几种健康牛阴道细菌中,多粒芽孢杆菌、嗜酸乳杆菌的细菌全菌液和过滤液对三种病原菌的拮抗作用最强,其中对链球菌和葡萄球菌呈高度敏感状态。 相似文献
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应用需氧和厌氧培养方法培养21头子宫内膜炎奶牛子宫液,将每头奶牛的病原菌在两个培养皿上重叠划线,贴上药敏纸片后在需氧和厌氧条件下进行多重药敏试验,筛选出敏感治疗药物,应用敏感药物对患牛进行了治疗,治愈率为80.9%。对所分离的细菌进行了鉴定,主要细菌为化脓性放线杆菌、大肠杆菌、链球菌、葡萄球菌和芽孢杆菌。 相似文献
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In 4 dairy farms located in Baotou area, clinical medicine investigation and drug resistance of pathogenic bacteria were conducted, and then curative effects of different treatment methods were analyzed.Epidemic materials were collected from diary cows infected by endometritis, from which pathogenic bacteria were isolated using conventional microbiology technologies.Broth microdilution method was used to detect the sensitivity and resistance of pathogenic bacteria to antibiotics commonly used in clinic.On this basis, the sensitive drugs were used to treat mastitis or endometritis, the clinical curative effect of traditional treatment group was compared with that of therapeutic test group.The result of traditional treatment group showed 13 antibiotics were used to treat 160 cases of endometritis, florfenicol was most effective, penicillin was most ineffective.The results of therapeutic test group showed that Staphylococcus aureus, Escherichia coli and Streptococcus were the main pathogenic bacteria of endometritis, which were sensitive to gentamicin and cefalotin, etc.The results of statistical analysis showed that the curative effect of therapeutic test group was extremely significantly different from that of traditional treatment group(P<0.01).Sensitive drugs screened by drug sensitive test could be used to target therapy, and obtain satisfied curative effect.Moreover, this approach could avoid antibiotic abuse. 相似文献
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通过对包头地区4个奶牛场开展奶牛子宫内膜炎临床用药调查研究和病原菌的耐药性分析,分析不同诊疗方法的治疗效果。采用常规微生物学技术分离了包头地区子宫内膜炎患牛的病原菌,采用微量肉汤稀释法测定了病原菌对临床上常用抗菌药物的敏感性和耐药性,在此基础上选用敏感性高的药物进行治疗试验,比较传统治疗组与治疗试验组两者的临床疗效。传统治疗结果显示,使用13种抗菌药物治疗160例子宫内膜炎病例,其中糜宫速康(氟苯尼考)最有效,青霉素类效果最差。治疗试验结果显示,金黄色葡萄球菌、大肠杆菌、链球菌等是引起子宫内膜炎的主要病原,且分离菌株对庆大霉素、头孢噻吩等抗菌药物敏感。统计分析结果显示,治疗试验组与传统治疗组疗效差异极显著(P<0.01)。采用药敏试验筛选的敏感药物进行针对性治疗,能获得满意的治疗效果,且该方法可避免药物滥用。 相似文献
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Bacteria on the genital mucosa have been studied less in healthy, non-puerperal cows than in cows with puerperal endometritis. We have thus analysed bacteria in swabs from the vagina and cervix of post-puerperal cattle (n = 644). Out of the animals, 6.8% had aborted within the last 12 months, 2.6% and 11.6% showed signs of vaginitis and endometritis, respectively. In 17.2% of cervical swabs pathogenic gram-positive and in 11.5% pathogenic gram-negative bacteria were found. Arcanobacterium pyogenes was isolated from 41.3% of cows with endometritis and from 3.5% without endometritis (p < 0.05). From 12.5% of cows with abortion but from no cow without abortion, Staphylococcus aureus was recovered (p < 0.05). Out of 383 vaginal swabs, 88.3% were positive. In 3.4% of swabs pathogenic gram-positive and in 16.7% pathogenic gram-negative microorganisms were found. The percentage of positive vaginal swabs did not differ between pregnant and non-pregnant animals. In the genital tract, the percentage of swabs positive for normal mucosal bacteria decreased from caudally to cranially (p < 0.05). Pathogenic bacteria were found more often in cervical than in vaginal swabs (p < 0.05). In conclusion, bacteria on the vaginal and cervical mucosa in cattle involve a wide range of species. In animals without endometritis or vaginitis, colonization of the mucosa rather than infection has to be assumed. 相似文献
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In six healthy mares and 24 mares showing reproductive disorders swab samples were taken from the fossa clitoridis to isolate Taylorella equigenitalis, and from the uterus to isolate mycoplasmas, ureaplasmas and other aerobic bacteria. Swab samples were also taken from the uterus for Chlamydia antigen ELISA and Chlamydia PCR studies. The uterus of 27 mares was examined cytologically, and biopsy samples were taken from the endometrium for histological examinations and for immunohistochemical examinations aimed at the detection of chlamydiae. T. equigenitalis, mycoplasmas, ureaplasmas and chlamydiae could not be detected from any of the mares examined. Aerobic facultative pathogenic bacteria were isolated from mares with endometritis in four cases. In 18 out of 22 mares with endometritis (82%) no infective agents could be demonstrated. Further studies are needed to elucidate the relative importance of non-infectious causes of endometritis and of anaerobic bacteria often detectable in the uterus in the aetiology of the reproductive disorders observed. 相似文献
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An investigation of the aetiological role of bovine herpesvirus 4 in bovine endometritis 总被引：2，自引：0，他引：2
Fábián K Makrai L Sachse K Szeredi L Egyed L 《Veterinary journal (London, England : 1997)》2008,177(2):289-292
Uteri from 31 infertile cattle were examined for the presence of bovine herpesvirus 4 (BoHV-4) by nested polymerase chain reaction (PCR). Samples were also tested for bacteria, including chlamydiae and Mycoplasma bovis. BoHV-4 was detected by PCR in 27/31 (87.1%) samples, but the presence and amount of viral DNA was not correlated with histological and bacteriological findings. Arcanobacterium pyogenes, Histophilus somni and Pasteurella multocida were isolated from five cows with endometritis. Chlamydiae were detected in four cases (12.9%), but only two of these had endometritis. The study does not support a role for BoHV-4 as primary agent in bovine endometritis. 相似文献
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桉油精的抗菌抗炎作用研究 总被引：1，自引：0，他引：1
为研究桉油精的抗菌抗炎作用,选取37株奶牛子宫内膜炎临床分离株,采用二倍稀释法测定其最小抑菌浓度(MIC)及最低杀菌浓度(MBC),同时分别设计小鼠耳肿胀和腹腔毛细血管通透性增加2种炎症模型研究其抗炎活性.结果显示,桉油精对7种细菌均有抗菌作用,MIC为3.9～12.4 mg/mL,MBC为6.0 ～ 18.2 mg/mL;桉油精能够显著抑制小鼠耳肿胀及腹腔毛细血管通透性增加,并且给药剂量为400 mg/kg时效果最佳.研究表明,桉油精对奶牛子宫内膜炎致病菌具有良好的抗菌作用和显著的抗炎效果. 相似文献
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Macroscopic examination of 487 ewe reproductive tracts from 18 different sources was made at Perth abattoirs. Of these, 194 were examined microscopically and 217 bacteriologically. Observations were made during March to May, when ewes were not grazing oestrogenic pasture. Significant lesions in ovaries or oviducts were rare. Macroscopic cysts were found in the uterus or cervix of 159 ewes (32.7%). Microscopic lesions, including uterine or cervical cysts and squamous metaplasia or goblet cell hyperplasia of the epithelium, were observed in 76% of ewes studied. Bacteria were isolated from 59 ewes, the most common genera being Corynebacterium, Escherichia, Streptococcus and Bacillus. Escherichia coli and streptococci appeared to be the most commonly associated with endometritis. Presence of bacteria was closely associated with endometritis and with cysts in the cervix. Endometritis was observed microscopically in 48 ewes (24%) and was significantly associated with the presence of cysts in the uterus or cervix. These results suggest that there is a high incidence of clover disease lesions in West Australian ewes, and that under commercial conditions clover disease may cause functional damage to the cervix, allowing passage of bacteria and resulting in endometritis. 相似文献
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【目的】 从健康奶牛生殖道分离筛选益生乳酸菌,为未来防治奶牛子宫内膜炎提供益生菌菌株并为开发防治此病的微生态制剂奠定基础。【方法】 采集10份健康奶牛的生殖道分泌物样品,用MRS培养基进行分离培养,通过形态学观察和16S rDNA序列对分离出的菌株进行鉴定,将所得序列结果与NCBI核酸数据库参考菌株进行相似性比对,确定各分离菌株的种属。利用牛津杯法分离筛选出具有较好抑菌活性的菌株,研究这些筛选出的菌株的生长曲线、产酸能力及抗菌药敏感性,并对其主要抗菌物质进行检测。【结果】 从10个样品中共分离出13株乳酸菌,经染色镜检和16S rDNA测序分析鉴定出13株乳酸菌,分别为植物乳杆菌11株、殊异肠球菌和鼠肠球菌各1株。单株抑菌试验得到5株对大肠杆菌抑菌能力较好的乳酸菌,5株乳酸菌生长特性及产酸能力均良好,对氨苄西林、四环素、红霉素、利福平、甲氧苄啶、氯霉素和青霉素7种抗菌药物敏感性较强。其中21和35号菌株的抗菌物质为过氧化氢和细菌素,而25、33和34号菌株的抗菌物质除过氧化氢和细菌素外还包括有机酸。【结论】 从奶牛生殖道中分离得到的3株乳酸菌有望作为微生态制剂用于奶牛子宫内膜炎的临床防治,其具体防治效果有待进一步探究。 相似文献
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本研究对两例确诊为化脓性子宫内膜炎的犬进行了病原的研究。用阴道采样法采集子宫分泌物进行细菌培养和生化鉴定,结果第1例因连续使用抗生素,而分离不到细菌;第2例分离到葡萄球菌和链球菌;药敏试验结果表明,犬子宫内膜炎的主要病原菌对临床使用较多的青霉素和链霉素有很大的耐药性,而对头孢唑啉、环丙沙星、恩诺沙星等药物较为敏感,为临床首选药物。 相似文献