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1.
作者报导用碘标记弓形虫病小鼠腹腔液分泌抗原对流免疫电泳新方法（ＲＣＩＥＰ）直接检测受检猪特异性抗体。ＲＣＩＥＰ与常规间接血凝（ＩＨＡ）对５０例病猪血清和５０例健康猪血清进行对比试验，结果证实该法具有高度的敏感性、特异性和较好的重现性。在不同稀释度时，弓形虫病猪血清ＲＣＩＥＰ和ＩＨＡ的阳性率分别为９４～１００％（平均９８．８０％）和２２～１００％（平均５８．８０％），健康猪的假阳性率前者未出现，而后者有１０％以上。从患病猪血清和抗原稀释度试验证实，ＲＣＩＥＰ的敏感性较酶标对流（ＥＬＣＥＰ）和常规ＣＩＥＰ分别高１５倍和４００倍。因此作者认为ＲＣＩＥＰ可作为猪弓形虫病的一种特异诊断新方法。 相似文献
2.
聚醛化聚苯乙稀（PAPS）血吸虫诊断液［‘］特异性强、敏感性高、重复性好。用其诊断家畜血吸虫病，快速、简便。现介绍如下：血吸虫的血清学诊断，王继玉等报道，应用间接血凝诊断血吸虫病，阳性率为92．2％，假阳性率为2．7％‘’］。沈杰等报道，应用酶联免疫吸附试验诊断血吸虫病，阳性率为97．7％，假阳性率为1．8％［‘］。应用环状沉淀法诊断血吸虫病，阳性率为100％，假阳性率为8％’‘］’。笔者应用PAPS血吸虫快速诊断液的阳性检出率为98．83％，假阳性率为0．33％。同时可以与其它的寄生虫病的P＊＊S系列诊断试剂配合应用［… 相似文献
3.
猪瘟Ｄｏｔ－ＥＬＩＳＡ诊断试剂盒的研究黄骏明，朱庆虎（中国农科院哈尔滨兽医研究所）应用与猪瘟病毒有共同可溶性ＰＰ抗原的牛病毒性腹泻病毒ＯｒｅｇｏｎＣ。；Ｖ毒株制备抗原，进行免疫斑点试验（ＤＯｔ－ＥＬＩＳＡ）已成功地用于猪瘟血清抗体的监测［、’］。为了... 相似文献
4.
为了了解循化县山羊布氏杆菌病（以下简称布病）和衣原体病的感染情况，笔者于１９９６年布氏杆菌试管凝集试验（ＳＡＴ）和衣原体间接血凝试验（ＩＨＡ）对６８６只１岁以上的山羊进行了血清学调查，现将结果报告如下。１ 材料与方法１．１ 被检血清：采自循化县未免疫的一岁以上的山羊。１．２ 诊断用试剂：布氏杆菌ＳＡＴ抗原，阳性血清和阴性血清系成都生物制品厂提供，批号为９６０４１８，稀释液用市售０．９％生理盐水。衣原体ＩＨＡ抗原阳性血清、阴性血清和特定稀释液均系中国农业科学院兰州兽医所提供，批号为９６０５２… 相似文献
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ＥＬＩＳＡ的放大系统杨永钦（云南省畜牧兽医研究所，６５０２２４）ＥＬＩＳＡ广泛用于抗原抗体的分析检测，八十年代以来，研究者从多方面探索了提高ＥＬＩＳＡ敏感性方法，包括选择理想的标记酶［１］，用荧光法检测标记酶的活性［２］，使用高亲和力的抗体及引进放大... 相似文献
6.
采用阴离子交换剂DEAE纤维素分离动物血液内锥虫获得纯净冻干锥虫粉末制备IHA诊断液，其纯度高、抗原省、效价高。经试验，该诊断液特异性强，敏感性强，检出率亦高，对疫区1024头牛进行检测，血纸检出率为6．8%，血清检出率为21．7%，两者的符合率为97．1%。 相似文献
7.
盐析法提纯弓形虫抗原ＥＬＩＳＡ诊断试剂盒的应用效果崔君兆，郑挺，谢丽群，吕元聪，章玲珠（广西卫生防疫站南宁５３００２１）目前国内检测弓形体抗体的ＥＬＩＳＡ试剂盒一般是用虫体裂解抗原￣［６，７，８］，存在着抗原产量少，制备过程复杂，不利推广等缺点，虽有... 相似文献
8.
采用四种方法：即①单克隆抗体检测抗原的酶联免疫吸附试验（Ａｇ－ＥＬＩＳＡ）②检测抗体的酶联免疫吸附试验（Ａｂ－ＥＵＳＡ）③表膜检查（ＢＣＥ）④小白鼠接种（ＭＩ），检查了肯尼亚１８３头骆驼锥虫的循环抗原，ＢＣＥ查出３７头阳性２０％），ＭＩ６０头阳性（３３％），Ａｇ－ＥＬＩＳＡ６３头阳性（３４％），Ａｂ－ＥＬＩＳＡ检出９０头阳性（４９％）。其中ＢＣＥ未能检出的２４头中，Ａｇ－ＥＬＩＳＡ检出１８头（７５％）。所有阳性头数中，Ａｇ－ＥＬＩＳＡ检出９３％，Ａｂ－ＥＬＩＳＡ９５％。根据５５头的结果，阳性与阴性之间Ａｇ－ＥＬＩＳＡＯＤ值差异极显著（Ｐ＜０．０００１），Ａｂ－ＥＬＩＳＡＯＤ值差异不显著。因此，用Ａｇ－ＥＬＩＳＡ或结合ＢＣＥ诊断伊氏锥虫感染比用ＡＢ－ＥＬＩＳＡ更理想。 相似文献
9.
盐酸锥双净（Ｔ－４６）给药局部刺激性和吸收毒性试验钟淑梅，沈杰，周勇志，王云飞，邱巧平，汪英华（中国农科院上海家畜寄生虫病研究所）盐酸锥双净（简称Ｔ－４６）是我所合成和筛选的抗锥虫新药，经ＣＤ５０、ＥＤ５０治疗指数测定［１］、亚急性毒性试验［２］、体... 相似文献
10.
采用淋巴细胞亲交瘤技术，研制出的抗衣原体单克隆抗体，应用酶联免疫吸附试验（ＥＬＩＳＡ）方法，对本地区所采集的１２８份羊血清、９０份牛血清进行检测，结果，羊血清阳性检出率为２５．８％，牛血清阳性检出率为３２．２％，选择５０份羊血清、５０份牛血清用已建立的检测衣原体抗体ＥＬＩＳＡ与间接血凝试验（ＩＨＡ）进行比较，牛血清ＥＬＩＳＡ阳性检出率为３２．０％，ＩＨＡ为２４．０％，ＥＬＩＳＡ比ＩＨＡ试验高出８个百分点；羊血清ＥＬＩＳＡ阳性检出率为２６．０％，ＩＨＡ为２２．０％，ＥＬＩＳＡ比ＩＨＡ高出４个百分点。 相似文献
11.
In late December 1985, 1485 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1985 and 44% of them responded. Veterinarians reported that 137,300 dogs were blood-tested to check for microfilariae and 1210 dogs were found with heartworm. Another 36 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number diagnosed in 1985 as 1247 (0.91%). Heartworm was reported from all provinces except Prince Edward Island, Newfoundland and Saskatchewan but most (1126) of the cases were in Ontario. Southwestern Ontario continued to be the primary focus of the infection in Canada. From Quebec, 91 cases were reported mostly from and around Montreal. From Manitoba, 19 cases were reported from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 28% of the cases were observed with clinical signs of heartworm disease and 78% had a history of not having left Canada. 相似文献
12.
In late November 1988, 1581 small and mixed animal clinics and institutional veterinarians across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1988, and 46% of them responded. Veterinarians reported that 181,577 dogs were blood-tested for heartworm disease and 367 dogs were found with D. immitis microfilariae. Another 60 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1988 as 441 (0.24%). 相似文献
13.
In late December 1984, 1853 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1984 and 35% of them responded. Veterinarians reported that 97,794 dogs were blood-tested to check for microfilariae and 1417 dogs (1.45% of those tested) were found with heartworm. Another 34 dogs were amicrofilaremic, but were diagnosed as having heartworm disease, to give the total number diagnosed in 1984 as 1451 (1.48%). Heartworm was reported from all provinces except Prince Edward Island and Newfoundland but most (1310) of the cases were in Ontario. In Quebec, 126 cases were reported mostly from west of Montreal. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 27% of the cases were observed with clinical signs of heartworm disease and 72% had a history of not having left Canada. Southwestern Ontario continued to be the primary focus of the infection. 相似文献
15.
In late November 1991, 1883 clinics in Canada were sent a questionnaire to assess the status of Dirofilaria immitis in dogs in 1991 and there was a 60.0% response. There were 344,031 dogs tested for heart-worm (HW), 627 were found infected and the prevalence of HW infection was 0.18%. There were 417 dogs with HW in Ontario, 116 in Manitoba, 38 in Quebec, 53 in British Columbia, three in Alberta, and one in Nova Scotia. In British Columbia, all of the infected dogs but one were from the Okanagan valley which, as from 1991, is a new focus of infection in Canada. Most dogs with HW had not been on preventive medication in 1990, and the prevalence among dogs tested and unprotected was 0.59%. That prevalence was considerably higher in endemic areas. Companion dogs, over three years of age and maintained primarily outdoors in rural areas, were most frequently infected. One cat was diagnosed with D. immitis and 33 dogs had Dipetalonema reconditium. 相似文献
16.
In late November 1989, 1732 clinics and institutional veterinarians were sent a questionnaire to assess the status of Dirofilaria immitis, and 51.7% responded. Of 247,716 dogs tested, 394 had D. immitis microfilariae and 51 were amicrofilaremic for a total of 445 cases and heartworm prevalence of 0.17%. Most (408) of these dogs had no preventive medication and the prevalence among dogs tested and unprotected was 1.01%. That prevalence was considerably higher in endemic areas. Thirty-seven dogs with heartworm had preventive medication. Heartworm was most frequent in companion dogs over three years of age maintained outdoors in rural areas. About 75% of the cases had never left Canada, 26% had clinical signs and 125 were not treated. Heartworm was reported from British Columbia, Manitoba, Ontario, Quebec, Nova Scotia and Newfoundland, but 383 cases were in Ontario. South-western Ontario was the primary focus of infection. There were 33 cases in Quebec and 24 in Manitoba, mainly found in and around Metropolitan Montreal and Winnipeg respectively. 相似文献
17.
In late November 1987, 1246 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1987, and 50% of them responded. Veterinarians reported that 165,428 dogs were blood tested for heartworm disease and 511 dogs were found with D. immitis microfilariae. Another 78 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1987 as 589 (0.35%). 相似文献
18.
In late December 1986, 1224 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1986; 46% of them responded. Veterinarians reported that 150,989 dogs were blood-tested for microfilariae and 869 dogs were found with heartworm. Another 65 dogs were amicrofilaremic but diagnosed with heartworm disease and one was found with heartworm at necropsy to give the total number diagnosed in 1986 as 935 (0.62%). Heartworm was reported from Manitoba, New Brunswick, Ontario and Quebec, but most (810) of the cases were from Ontario. South-western Ontario continued to be the primary focus of the infection in Canada. There were 103 cases reported from Quebec, mostly from and around Montreal, and 21 cases from Manitoba, from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 33% of the cases were observed with clinical signs of heartworm disease and 81% had a history of not having left Canada. 相似文献
20.
In late December 1983, 2 800 veterinarians across Canada were sent a questionnaire in order to assess the status of heartworm disease in Canada in 1983 and 26% of them responded. Veterinarians reported that 59 504 dogs were blood-tested to check for microfilariae and 771 dogs (1.30% of those tested) were found with Dirofilaria immitis. Heartworm disease was diagnosed in all provinces except New Brunswick and Newfoundland but most (733) of the cases were in Ontario. Heartworm disease was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 31% of the cases were observed with clinical signs of heartworm disease and 64% had a history of not having left Canada. Southwestern Ontario continues to be the focus of the infection and most of the dogs there had not left the province previously. 相似文献
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