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酮病奶牛血清中NO含量及NOS活性变化的研究 总被引：2，自引：0，他引：2
为了深入研究酮病的发生机理，选择荷斯坦奶牛作实验动物，应用酮粉法和改良式水杨醛比色法随机检测分组，Ⅰ组为10头阳性牛、Ⅱ组为10头对照牛。应用硝酸还原酶法测定血清中一氧化氮(NO)含量和一氧化氮合酶(NOS)活性。实验结果表明对照组奶牛血清中的NO含量及NOS活性均显著大于阳性组。由此可知，酮病可导致奶牛血清中NO含量降低、NOS活性下降。 相似文献
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荷斯坦奶牛酮病对抗氧化系统影响的研究 总被引：5，自引：1，他引：4
为了探讨酮病对抗氧化系统的影响 ,以荷斯坦奶牛为实验动物 ,应用酮粉法和改良式水杨醛比色法随机检测结果 ,一组为 1 0头阳性牛 ,另一组为 1 0头对照牛。应用DTNB显色法测定血清中GSH Px活力 ,应用黄嘌呤氧化法测定血清中T SOD活力 ,应用硫代巴比妥酸法测定血清中MDA含量。结果表明 ,酮病奶牛血清中GSH Px活力极显著降低 (P <0 .0 1 )、T SOD活力显著降低 (P<0 .0 5) ,血清中MDA含量显著增高 (P <0 .0 5)。因此酮病影响奶牛抗氧化系统 ,这一结论对揭示奶牛酮病的发生机理具有重要的意义 相似文献
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《中国奶牛》2016,(8)
为探究奶牛酮病治疗对机体总抗氧化能力的影响,本研究选取南宁市某规模化养殖场的65头围产期奶牛作为研究对象,经血浆酮体定量检测,从中选取5头健康牛作为健康对照组,将检出的10头酮病患牛分为治疗组(5头)和阳性对照组(5头)。试验期间,对治疗组进行药物治疗,阳性对照组和健康对照组不做任何治疗。对各组奶牛分别于治疗前和停药后清晨空腹颈静脉采血,测定血浆酮体(改良水杨醛比色法)、血糖(氧化酶-过氧化物酶法)和TAC(铁还原比色法)水平。结果显示,治疗后,治疗组奶牛的血酮含量较治疗前极显著下降,血糖和总抗氧化能力含量极显著升高。阳性对照组和健康对照组的血酮和血糖含量、总抗氧化能力治疗前后变化不大,差异均不显著。结果表明,酮病治疗可提高奶牛血浆TAC水平,提示TAC的提高与酮病痊愈有关。 相似文献
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本试验旨在研究荷斯坦奶牛亚临床酮病对血清中微量元素的影响,选择40头产后1～15 d的荷斯坦亚临床酮病奶牛,通过应用原子吸收光谱法测定血清中Cu、Fe、Mn、Zn、Se、Co含量,应用分光光度计和比色法测定血清中GSH-Px、T-AOC、T-SOD活力及MDA含量。结果显示,阳性组血清中Cu、Zn、Se、Co的含量在整个检测过程中均极显著低于对照组(P＜0.01);阳性组血清中Mn的含量在第1、5天的检测中显著低于对照组(P＜0.05),第10、15天极显著低于对照组(P＜0.01);阳性组血清中Fe的含量与对照组相比差异不显著（P＞0.05）;阳性组血清中GSH-Px、T-SOD、T-AOC的活性均极显著低于对照组(P＜0.01);阳性组血清中MDA的含量极显著高于对照组(P＜0.01)。结果表明,亚临床酮病直接影响奶牛抗氧化系统GSH-Px、T-AOC、T-SOD的活性,间接地影响血清中微量元素Cu、Mn、Se、Zn的含量。Co是维生素B12的重要组成部分,维生素B12在肝脏内的浓度下降,从而导致亚临床酮病血清中Co的含量降低。亚临床酮病奶牛肝脏和氧化酶受损,破坏Fe的结合,机体内贮存的Fe则可以释放出来,维持机体的需要,血清中Fe的含量没有显著的变化。 相似文献
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为了查明河南省新郑县耕牛发生“倒卧病”的病因,用核固红比色法、氧化亚锡法、达旦黄比色法,分别对34头临床健康牛和7头疑似钙、磷代谢障碍的病牛进行了血清钙、磷、镁含量测定。结果健康牛的钙、磷比为2.6:1,病牛为4.6:1,差异显著,表明血清钙、磷比例失调是导致耕牛发生“倒卧病”的主要原因。 相似文献
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《畜牧兽医杂志》2019,(6)
对沈阳市某乳业公司一个存栏700头奶牛的奶牛场进行血清钙、磷、葡萄糖的营养代谢状况抽测。随机抽取12头产奶牛血清,分别测定了血清钙,血清无机磷,血清葡萄糖的浓度生化指标。奶牛的血清钙含量为3.82～10.88 mg/100 mL,平均值为:7.15 mg/100 mL;血清无机磷含量为3.82～8.13 mg/100 mL,平均值为:5.91 mg/100 mL;血糖含量为21.8～68.1 mg/100 mL,平均值45.0 mg/100 mL。结果表明:奶牛的血钙、磷比例失衡,钙磷积偏低,血清钙含量水平较低,血糖含量处于低水平状态,这将增加了奶牛患有骨营养不良、生产瘫痪、奶牛酮血症的危险性,并讨论了血液生化指标的变化对诊断和监测牛群代谢的动态意义。 相似文献
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为了解酮病复方制剂(KTP)对荷斯坦奶牛酮病的治疗效果,本研究经临床和实验室检查筛选,选取广西某牛场16头荷斯坦奶牛,酮病组奶牛8头,临床健康奶牛8头作为对照组,按照治疗方案,对酮病奶牛每天灌服400mL KTP治疗,连续5天,对照牛不做处理。结果表明,在治疗前,酮病组血酮含量极显著高于对照组,血糖含量极显著低于对照组(p<0.01),酮病组的血酮含量在治疗后极显著低于治疗前,血糖含量极显著高于治疗前(p<0.01)。治疗后两组牛血糖、血酮差异不显著(p>0.05),KTP对奶牛酮病具有显著的治疗效果。 相似文献
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选择产奶量、胎次、分娩时间相近的荷斯坦奶牛12头,采用单因子随机分组设计,试验分A、B、C3组,A组为对照组饲喂基础日粮,B组为基础日粮+100g脂肪酸钙,C组为基础日粮+200g脂肪酸钙。每周测定一次血清钙、磷和羟脯氨酸的含量。结果奶牛血清磷和羟脯氨酸的含量差异不显著(P>0.05),添加200g脂肪酸钙,血清钙在4周后显著升高(P<0.05)。表明:脂肪酸钙不影响奶牛血清磷和血清羟脯氨酸的含量。在日粮中添加200g脂肪酸钙4周以后,有利于促进机体对钙的吸收。 相似文献
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Ovine scrapie was first recorded in Cyprus in 1985. Subsequently four dairy goats kept in two mixed flocks with affected sheep developed characteristic clinical signs similar to those seen in sheep. Fifteen goats from the two flocks were examined histologically and neurological lesions consistent with a diagnosis of scrapie were found in the four animals and in three others which had subsequently developed early neurological signs. These lesions were similar to those of naturally-affected sheep although neuronal degeneration and vacuolation were more severe in some cases. 相似文献
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Owen J Slocombe D McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1986,27(9):324-328
In late December 1985, 1485 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1985 and 44% of them responded. Veterinarians reported that 137,300 dogs were blood-tested to check for microfilariae and 1210 dogs were found with heartworm. Another 36 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number diagnosed in 1985 as 1247 (0.91%).
Heartworm was reported from all provinces except Prince Edward Island, Newfoundland and Saskatchewan but most (1126) of the cases were in Ontario. Southwestern Ontario continued to be the primary focus of the infection in Canada. From Quebec, 91 cases were reported mostly from and around Montreal. From Manitoba, 19 cases were reported from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 28% of the cases were observed with clinical signs of heartworm disease and 78% had a history of not having left Canada.
相似文献14.
Slocombe JO McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1989,30(6):504-508
In late November 1988, 1581 small and mixed animal clinics and institutional veterinarians across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1988, and 46% of them responded. Veterinarians reported that 181,577 dogs were blood-tested for heartworm disease and 367 dogs were found with D. immitis microfilariae. Another 60 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1988 as 441 (0.24%). 相似文献
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Slocombe JO McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1985,26(10):323-327
In late December 1984, 1853 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1984 and 35% of them responded. Veterinarians reported that 97,794 dogs were blood-tested to check for microfilariae and 1417 dogs (1.45% of those tested) were found with heartworm. Another 34 dogs were amicrofilaremic, but were diagnosed as having heartworm disease, to give the total number diagnosed in 1984 as 1451 (1.48%). Heartworm was reported from all provinces except Prince Edward Island and Newfoundland but most (1310) of the cases were in Ontario. In Quebec, 126 cases were reported mostly from west of Montreal.
Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 27% of the cases were observed with clinical signs of heartworm disease and 72% had a history of not having left Canada. Southwestern Ontario continued to be the primary focus of the infection.
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Slocombe JO Villeneuve A 《The Canadian veterinary journal. La revue veterinaire canadienne》1993,34(10):630-633
In late November 1991, 1883 clinics in Canada were sent a questionnaire to assess the status of Dirofilaria immitis in dogs in 1991 and there was a 60.0% response. There were 344,031 dogs tested for heart-worm (HW), 627 were found infected and the prevalence of HW infection was 0.18%. There were 417 dogs with HW in Ontario, 116 in Manitoba, 38 in Quebec, 53 in British Columbia, three in Alberta, and one in Nova Scotia. In British Columbia, all of the infected dogs but one were from the Okanagan valley which, as from 1991, is a new focus of infection in Canada. Most dogs with HW had not been on preventive medication in 1990, and the prevalence among dogs tested and unprotected was 0.59%. That prevalence was considerably higher in endemic areas. Companion dogs, over three years of age and maintained primarily outdoors in rural areas, were most frequently infected. One cat was diagnosed with D. immitis and 33 dogs had Dipetalonema reconditium. 相似文献
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In late November 1989, 1732 clinics and institutional veterinarians were sent a questionnaire to assess the status of Dirofilaria immitis, and 51.7% responded. Of 247,716 dogs tested, 394 had D. immitis microfilariae and 51 were amicrofilaremic for a total of 445 cases and heartworm prevalence of 0.17%. Most (408) of these dogs had no preventive medication and the prevalence among dogs tested and unprotected was 1.01%. That prevalence was considerably higher in endemic areas. Thirty-seven dogs with heartworm had preventive medication. Heartworm was most frequent in companion dogs over three years of age maintained outdoors in rural areas. About 75% of the cases had never left Canada, 26% had clinical signs and 125 were not treated.
Heartworm was reported from British Columbia, Manitoba, Ontario, Quebec, Nova Scotia and Newfoundland, but 383 cases were in Ontario. South-western Ontario was the primary focus of infection. There were 33 cases in Quebec and 24 in Manitoba, mainly found in and around Metropolitan Montreal and Winnipeg respectively.
相似文献19.
Slocombe JO McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1988,29(8):641-646
In late November 1987, 1246 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1987, and 50% of them responded. Veterinarians reported that 165,428 dogs were blood tested for heartworm disease and 511 dogs were found with D. immitis microfilariae. Another 78 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1987 as 589 (0.35%). 相似文献
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Owen J Slocombe D McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1987,28(8):491-495
In late December 1986, 1224 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1986; 46% of them responded. Veterinarians reported that 150,989 dogs were blood-tested for microfilariae and 869 dogs were found with heartworm. Another 65 dogs were amicrofilaremic but diagnosed with heartworm disease and one was found with heartworm at necropsy to give the total number diagnosed in 1986 as 935 (0.62%).
Heartworm was reported from Manitoba, New Brunswick, Ontario and Quebec, but most (810) of the cases were from Ontario. South-western Ontario continued to be the primary focus of the infection in Canada. There were 103 cases reported from Quebec, mostly from and around Montreal, and 21 cases from Manitoba, from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 33% of the cases were observed with clinical signs of heartworm disease and 81% had a history of not having left Canada.
相似文献