共查询到18条相似文献，搜索用时 234 毫秒
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采用水提醇沉法提取了8种不同食用菌子实体的水溶性粗多糖,测定了样品中多糖、葡聚糖和β-葡聚糖的含量,结果表明,刺芹侧耳(Pleurotus eryngii)、毛头鬼伞(Coprinus comatus)、香菇(Lentinula edodes)和桑黄(Phellinus baumii)粗多糖中的多糖含量较高,香菇和姬松茸(Agaricus blazei)粗多糖中葡聚糖含量较高;猴头菌(Hericium erinaceus)、刺芹侧耳、香菇和姬松茸粗多糖中β-葡聚糖含量较高。采用截留分子量8000～12000透析袋纯化8种食用菌粗多糖,将纯化产物用于体外刺激巨噬细胞RAW264.7释放NO实验和脾淋巴细胞增殖实验,8种样品均能刺激巨噬细胞释放NO,其中香菇、刺芹侧耳和毛头鬼伞粗多糖在低浓度(200μg/mL)时,刺激巨噬细胞释放NO量即可达到40μmol/L以上,高浓度(500μg/mL)时高于阳性对照;体外脾淋巴细胞增殖实验结果显示,8种供试样品均能刺激脾淋巴细胞增殖,以香菇、刺芹侧耳和猴头菌粗多糖效果较好。实验结果证明这8种食用菌粗多糖均具有体外免疫活性。 相似文献
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探究刺芹侧耳(Pleurotus eryngii)、瓦尼桑黄(Sanghuangporous vaninii)、灰树花(Grifola frondosa)、赤芝(Ganoderma lucidum)、香菇(Lentinula edodes)、蟹味菇[Hypsizygus marmoreus (brown cultivar)]、白玉菇[H. marmoreus (white cultivar)]的子实体和樟芝(Antrodia cinnamomea)菌丝体水提物及醇提物对乙醇、H2O2和对乙酰氨基酚诱导肝细胞损伤的保护作用。研究结果表明：在乙醇诱导的肝损伤中,相较于乙醇组,质量浓度为25、50、100μg·mL-1的灰树花和樟芝醇提物和质量浓度为25、100、400μg·mL-1的蟹味菇、刺芹侧耳、灰树花、香菇、白玉菇水提物均可显著提高肝细胞存活率。在H2O2诱导的肝损伤中,相较于H2O2组,质量浓度为25、50、... 相似文献
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毛蜂窝菌提取物体外抗肿瘤活性的研究 总被引：1，自引：0，他引：1
以人肺癌细胞NCI-H460、中枢神经系统癌细胞SF-268及乳腺癌细胞MCF-7为供试细胞株,采用MTT(四甲基偶氮唑盐)法对毛蜂窝菌(Hexagona apiaria)野生子实体、栽培子实体、培养废料、发酵菌丝体的醇提物和发酵液乙酸乙酯萃取物、发酵液水相部分等各提取物进行了体外抗肿瘤活性研究.结果表明,毛蜂窝菌发酵液乙酸乙酯萃取物对三种肿瘤细胞株都有较明显的抑制作用,抑制率达80%以上;其它提取物也有一定的抑制作用,但抑制率在40%以下. 相似文献
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金耳菌丝体醇提物体外抗肿瘤抗病毒活性研究 总被引：1，自引：1，他引：0
采用体外肿瘤细胞株(小鼠白血病细胞株L1210、人乳腺癌细胞株MCF-7和人肠腺癌细胞株SW620)的增殖抑制试验模型和抗病毒(流感甲型病毒和疱疹病毒)的筛选模型,对3个金耳(Tremella aurantialba)菌株菌丝体醇提物活性进行比较研究.抗肿瘤试验结果表明,3种提取物材料在高浓度(200 μg/mL)时,对3个供试肿瘤细胞株的细胞增殖均有明显的抑制作用,其中以YK的抑制活性最高(对L1210、SW620和MCF-7的抑制率分别为89.4%,75.0%和64.1%);在中浓度(50 μg/mL)时,均表现出一定的抑制作用;而在低浓度(10 μg/mL)时,则均无明显的抑制作用.抗流感甲型病毒和抗疱疹病毒试验结果表明,只有YK对流感甲型病毒表现有抗病毒活性,但此活性较弱. 相似文献
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为筛选林芝松口蘑(Tricholoma matsutake)子实体中具抗肿瘤作用的组分,采用MTT法测定林芝松口蘑乙醇提取物的不同有机溶剂萃取分部对乳腺癌细胞(MCF-7)、胃癌细胞(SGC-7901)和肺癌细胞(A549)的体外抑制率。结果表明:林芝松口蘑乙醇提取物的抑癌活性物质主要集中在氯仿分部,100μg/mL作用48h,对MCF-7和SGC-7901的抑制率分别达到了64%和55%;乙醇提取物的各萃取分部对A549抑制效果较差,在实验浓度范围内抑制率均未超过25%。 相似文献
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为分析刺芹侧耳(Pleurotus eryngii)工厂化栽培中发黄腐烂子实体的细菌组成,构建16SrDNA文库,利用限制性内切酶MspI和RsaI分别对随机克隆进行酶切筛选,并测定代表性克隆16SrDNA序列,BLAST分析结果表明刺芹侧耳子实体病状组织中存有两类菌群,分别为假单胞菌和芽孢杆菌属。同时,对刺芹侧耳子实体病状组织中的细菌进行分离培养、致病性测定、16SrDNA鉴定及BLAST分析,病原细菌与恶臭假单胞菌(Pseudomonas putida)的同源性较高,其作为引起刺芹侧耳工厂化栽培细菌性病害的病原菌在国内外尚属首次报道。 相似文献
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高效阴离子色谱-脉冲安培检测法分析食用菌中海藻糖、甘露醇和阿糖醇 总被引：2，自引：0，他引：2
采用高效阴离子色谱-脉冲安培检测法(HAPEC-PAD)测定17种食用菌中海藻糖、甘露醇和阿糖醇含量.结果表明:阿糖醇、海藻糖和甘露醇的线性范围分别为239.62～1.25、59.41～0.15、148.29～5.00μg/mL,检出限分别为0.41、0.04、.11 μg/mL,定量限分别为1.14、0.10和5.79μg/mL;17种食用菌中,香菇(Lentinula edodes)和猴头菌(Hericium erinaceus)中阿糖醇含量较高,茶树菇(Agrocybe aegerita)、刺芹侧耳(Pleurotus eryngii)、鲍鱼侧耳(Pleurotus abalonus)、松口蘑(Tricholoma matsutake)、蛹虫草(Cordyceps militaris)中海藻糖含量较高,姬松茸(Agaricus blazei)、鸡油菌(Cantharellus cibarius)、金顶侧耳(Pleurotus citrinopileatus)、冬虫夏草(Cordyceps sinensis)中甘露醇含量较高;该项检测技术分离效果好、样品测定时不需要衍生化处理、检测时间短,是测定食用菌中功能性寡糖和糖醇类物质的有效方法. 相似文献
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AIM:To investigate the molecule mechanism of microRNA (miR)-30c over-expression inhibiting malignant phenotypes of cervical cancer cells. METHODS:Cervical cancer cell lines C33A, HeLa, SiHa and CaSki were transfected with pGenesil-1-miR-30c plasmid using Lipofectamine 2000 kit, and the expression of miR-30c was determined by TaqMan real-time PCR. The cell viability inhibition rate, colony formation ability, migration rate and apoptotic rate were measured by MTT assay, colony formation assay, Transwell experiment, and flow cytometry with Annexin V-FITC staining. The protein expression of Bax, Bcl-2, matrix metalloproteinase (MMP)-9, MMP-13 and tissue inhibitor of metalloprotei-nase-1 (TIMP-1) was detected by Western blot. RESULTS:The expression levels of miRNA-30c in the cervical cancer cell lines transfected with pGenesil-1-miR-30c plasmid were significantly higher than those in negative control groups (cell lines transfected with pGenesil-1 plasmid) (P<0.01). Significantly increased cell viability inhibition rate, and decreased colony formation ability and migration rate were found in the cervical cancer cell lines over-expressing miR-30c as compared with negative control groups (P<0.05). The apoptotic rate in the cervical cancer cell lines over-expressing miR-30c was dramatically higher than that in control groups (P<0.05). Over-expression of miR-30c in cervical cancer cells promoted the protein expression of Bax and TIMP-1, and decreased the protein expression of Bcl-2 and MMP-13 (P<0.05 or P<0.01). CONCLUSION:Over-expression of miR-30c significantly inhibits the viability and migration, and induces apoptosis of cervical cancer cells. The mechanism may be related to activating apoptosis pathway and inhibiting MMP-13 protein expression. 相似文献
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为了验证低分子姬松茸菌对人体健康的改善作用。在探讨姬松茸多糖的药用价值的基础上,对低分子姬松茸多糖的提取和纯化方法进行了试验,通过单因素试验与正交试验确定了提取最佳条件;对低分子姬松茸多糖抗肝癌HepG-2细胞的活性进行了试验,结果显示,低分子姬松茸多糖对肝癌HepG-2细胞的抵制率要高于普通多糖,但纯化后的低分子姬松茸多糖抑制率反而会降低,证明了多糖中的某些蛋白质具有一定的抗癌效果,抗癌活性与多糖的纯化和含量没有相关性。 相似文献
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以5种食药用菌水提取物为试材,采用测定糖苷酶抑制率的方法,研究了5种珍稀食药用菌水提取物对糖苷酶的抑制作用,以期为进一步开发降血糖药物提供参考依据。结果表明:羊肚菌、桑黄、灵芝、蝉花、虫草水提取物对4种糖苷酶均有不同程度的抑制,且差异显著(P<0.05)。5种水提物中,桑黄水提物对蔗糖酶的抑制率最高(74.8%),略低于常用的降糖药阿卡波糖以及米格列醇,虫草水提物对蔗糖酶的抑制率最低(35.9%)。虫草水提物对α-淀粉酶的抑制能力最高(9.7%)且显著高于米格列醇(P<0.05),羊肚菌水提物对α-淀粉酶的抑制最低(1.1%)。羊肚菌水提物对α-葡萄糖苷酶显示出较好的抑制能力(15.4%),显著高于阿卡波糖和米格列醇(P<0.05),而灵芝水提物对α-葡萄糖苷酶的抑制水平最低(11.2%)。灵芝水提物对麦芽糖酶显示出较高的抑制能力(10.2%),显著高于米格列醇(P<0.05),而与阿卡波糖相当,桑黄水提物对麦芽糖酶抑制最差(4.6%)。5种水提物在20~60℃以及pH 6~8时对4种糖苷酶仍然保持较好的抑制能力。 相似文献
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以瓜果腐霉（Pythium aphanidermatum）、辣椒疫霉（Phytophthora capsici）、尖镰孢菌（Fusarium oxysporium）、茄镰孢菌（Fusarium solani）、灰葡萄孢（Botrytis cinerea）、禾谷镰孢菌（Fusarium graminearum）等6种植物病原真菌为供试菌株,采用菌丝生长法对28种药用植物提取液的抑菌活性进行了测定。结果表明：丁香（Eugenia caryophyllata）对6种植物病原真菌的抑制率均达100%,乌梅（Armeniaca mume）、百部（Stemona sessilifolia）和木香（Aucklandia lappa）对5种植物病原真菌的抑制率均在70%以上。采用孢子萌发法对抑菌效果较好的5种药用植物进行进一步研究,结果表明：这5种药用植物对灰葡萄孢孢子萌发抑制率均在90%以上。 相似文献
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AIM: To investigate the effect and mechanisms of siRNA-hTERT-induced inhibition of Tca8113 tongue cancer cells in vitro and in vivo. METHODS: A small interference RNA (siRNA) targeting to hTERT mRNA (siRNA-hTERT1) was constructed. The siRNA was transfected into Tca8113 tongue cancer cells in vivo and in vitro with cationic liposome. A non-specific siRNA (siRNA-hTERT2) and non-treatment were used as negative control group and blank group. The cell growth in vitro was detected by MTT method. The cell apoptosis in vitro was analyzed by flow cytometry. The effect of siRNA-hTERT1 on xenografts in nude mice was observed by determining the tumor size. The cell apoptosis in xenografts was analyzed by Hoechst staining. The expressions of hTERT mRNA in vitro and in vivo were detected by RT- PCR. RESULTS: The inhibition rates of cell growth in vitro 72 h after siRNA-hTERT1 treatment was 47.2％, significantly higher than that in siRNA-hTERT2 treatment group (2.6％, P<0.01). The cell apoptosis rate was 27.30%±0.18% in vitro, significantly increased at 48 h after transfection of siRNA-hTERT1, compared to negative control group and blank group (P<0.01). The size of xenografts in siRNA-hTERT1 treatment group was (298.8±138.7)mm3, significantly smaller than that in siRNA-hTERT2 treatment group and blank group (495.1±151.6)mm3 and (506.8±207.4)mm3, the inhibition rate was 40.0% (P<0.01). The numbers of apoptotic cells in xenografts significantly increased after transfection of siRNA-hTERT1, compared to negative control group and blank group (P<0.01). Compared to negative control group and blank group, the expression of hTERT mRNA in Tca8113 tongue cancer cells in vitro and in vivo was inhibited by siRNA-hTERT1. CONCLUSION: siRNA-hTERT1 powerfully inhibits the growth of Tca8113 tongue cancer cells in vitro and in vivo. The specific inhibition of hTERT mRNA expression and cell apoptosis may be its main mechanisms. 相似文献
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