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1.
《Scientia Horticulturae》2005,104(1):79-89
The seasonal effect of soil water availability on leaf gas exchange of plantain plants cv. Hartón growing on two different texture soils (loamy and clayey) were evaluated. Soil water deficits corresponded to 48, 24 and 4 days without precipitation. Daily measurements of leaf gas exchange and microclimatic conditions were carried out at 2 h intervals in a humid tropical environment south of Maracaibo Lake, Venezuela. The results show that cv. Hartón is sensitive to conditions of low water deficit on loamy and to a much greater degree on clayey soils. A marked reduction in leaf conductance (gs) was observed under severe as well as moderate deficit (below 50 mmol m−2 s−1) on clayey soils. Under low deficit gs increases to values between 60 and 100 mmol m−2 s−1. The same trend was observed in plants on loamy soils but higher gs for all conditions were obtained compared with plants on clayey soil. Stomatal closure produced a reduction of 85 and 55% of total assimilation (Atot) for severe and moderate deficit in plants on clayey soils, respectively. While plants on loamy soil exhibited a 65 and 35% reduction, respectively. Water use efficiency (WUE) consistently decreased as available soil water decreased on both soil types. Independently of soil water conditions, higher WUE were always obtained for loamy soils. This suggests that cv. Hartón does not have the ability to adjust the CO2 assimilation to transpiration ratio in order to optimize gas exchange. This evidences the importance of maintaining high conditions of available soil water in order to avoid lower assimilation rates that probably influence negatively on yield and fruit quality.  相似文献   

2.
《Scientia Horticulturae》2002,95(3):239-249
Microcuttings (shoots each with two leaves) of Limonium ‘Misty Blue’ were cultivated in vitro for 28 days under photoautotrophic (sucrose-free culture medium; CO2 and photosynthetic photon flux (PPF) enriched conditions), photomixotrophic (medium with 30 g l−1 sucrose; CO2 and PPF enriched conditions) and heterotrophic (medium with 30 g l−1 sucrose; CO2 non-enriched conditions) methods. Several growth variables were measured during and at the end of cultivation: shoot fresh and dry weight, percentage of shoot dry matter, root fresh weight, number of leaves, leaf area, chlorophyll and sugar content of leaves, stomatal density and size, net photosynthetic rate (NPR) and percent survival of plantlets ex vitro. Plantlets grown in photoautotrophic and photomixotrophic methods had more leaves, high chlorophyll and sugar contents, high NPR, and showed high percent survival. However, these plantlets possessed less number of stomata per square millimeter. In contrast, the plantlets grown by the heterotrophic method showed decreased values of these growth variables except for the number of stomata per square millimeter. These results indicate that CO2 enrichment for plantlets in vitro at a relatively high PPF would promote photosynthesis and hence growth of chlorophyllous explants/plantlets in vitro. The resulting plantlets were acclimatized better and sooner on ex vitro transplantation.  相似文献   

3.
《Scientia Horticulturae》2005,105(2):197-211
CO2 concentration was monitored during three 15-day subculturing cycles in vessels containing actively proliferating plum cultures of Prunus cerasifera, clone Mr.S. 2/5. The effects of two photosynthetic photon flux density regimes: 50 ± 5 μmol m−2 s−1 and 210 ± 5 μmol m−2 s−1 were compared. Three distinct phases in the CO2 trend were distinguished during each culturing cycle of both light treatments. In the first, occurring at the beginning of the culture cycle, the amount of CO2 emitted by the cultures during dark periods was greater than that assimilated during the light periods. In the second phase, the opposite trend was detected, while in the third, the range of CO2 day–night fluctuations increased or remained stable according to the number of explants per vessel. The treatment with 210 ± 5 μmol m−2 s−1 did not modify the CO2 phase trend but induced more pronounced fluctuations in day–night CO2 concentration. Under this light treatment, cultures reached CO2 compensation point for a period as long as 48% of the total number of light hours, while under 50 ± 5 μmol m−2 s−1, it was only 8%. The different range in CO2 day–night fluctuations monitored throughout a subculturing cycle, appeared to be mainly induced by changes in culture growth dynamics.  相似文献   

4.
《Scientia Horticulturae》2001,87(3):155-170
Measurements of CO2 exchange of cauliflower leaves were carried out in a field experiment which included two nitrogen fertilisation rates. Irradiance and CO2 concentration were varied at the leaf level within a leaf cuvette and additionally a temperature treatment was applied to field grown plants moved into climate chambers. These measurements were used to estimate parameter values of a rectangular hyperbola describing cauliflower leaf CO2 exchange as a function of irradiance and CO2 concentration. The obtained parameter estimates were used to derive empirical regression equations with temperature and nitrogen content of the leaves as independent variables. The resulting relationships were applied within a simple photosynthesis–respiration based dry matter production model in order to derive functional relationships between light use efficiency and irradiance, leaf area index and temperature.The rectangular hyperbola was able to describe the gas exchange data as varied by irradiance and CO2 concentration on the single leaf level with sufficient accuracy, but estimates of initial light use efficiency (about 25 μg J−1) were too high because of the bias emanating from the limited flexibility of this model. Light saturated photosynthesis rate (Pmax) showed an optimum response to temperature and an increase with increasing nitrogen content of leaves. The initial slope α of the rectangular hyperbola showed no consistent responses to ambient temperature and nitrogen content of leaves. The respiration per unit leaf area β increased exponentially with increasing temperature, resulting in a Q10 value of 1.86. Because only a limited number of plants was evaluated in this study, additional work is needed to further substantiate the results of the gas exchange measurements.The model analysis demonstrated that LUE is independent of the light integral over a range 5–10 MJ m−2 per day photosynthetically active radiation if one assumes an adaptation of Pmax within the canopy and over time according to the incident irradiance. Acclimatisation within the canopy and higher leaf area indices, LAI, reduce the decrease of LUE with irradiance but a substantial decline remains even for LAI values of 4.  相似文献   

5.
《Scientia Horticulturae》2005,106(1):70-75
The objective of this study was the comparison of the effect of two Mn sources (MnSO4·H2O, MnEDTA) which were applied at various concentrations (0, 200, 400, 800, and 1200 mg Mn l−1) to the leaves of ‘Washington navel’ orange trees in order to correct Mn deficiency.One hundred and seventy days after the foliar application of Mn solutions, the mean Mn concentrations in the leaves treated with MnSO4·H2O (200, 400, 800 or 1200 mg Mn l−1) or MnEDTA (400, 800 or 1200 mg Mn l−1) were significantly higher than those of the control leaves. Manganese sulfate (MnSO4·H2O) was more effective than MnEDTA regarding the improvement of the leaf Mn concentrations of the trees, when applied at equal Mn concentrations. Finally, the leaf Mn concentrations were in the sufficiency range (>25 mg kg−1 d.w.), only after the application of 800 or 1200 mg Mn l−1 as MnSO4·H2O.  相似文献   

6.
《Scientia Horticulturae》2005,104(1):25-36
We studied the effects of Fe deficiency on physiological parameters of citrus rootstocks grown in nutrient solution. Three 4-week old rootstocks (‘Troyer’ citrange – Citrus sinensis (L.) Osb. × Poncirus trifoliata (L.) Raf., Citrus taiwanica Tan. and Shim., and ‘Swingle’ citrumelo – Poncirus trifoliata (L.) Raf. × Citrus paradisi Macf.) were grown in nutrient solutions with 0, 5, 10, 15 and 20 μmol Fe dm−3. Calcium carbonate (1 g dm−3) was added to all solutions to mimic the natural conditions in calcareous soils. For each rootstock, shoot length, number of leaves, and root and shoot dry weights were measured at the end of experiment. Chlorophyll concentration was estimated using a portable SPAD-502 meter calibrated for each rootstock. The amount of nutrients (P, K, Mg, Ca, Fe, Zn, Mn, and Cu) was determined in shoots. Chlorophyll fluorescence parameters (F0: basal fluorescence; Fm: maximum fluorescence; Fv = FmF0: variable fluorescence) were measured with a portable fluorimeter. ‘Troyer’ citrange rootstock was the most tolerant to Fe deficiency. These plants grew more and accumulated more chlorophyll and nutrients than the others in the presence of low levels of Fe (10 μmol Fe dm−3). ‘Swingle’ citrumelo plants needed 20 μmol Fe dm−3 in the nutrient solution to secure adequate growth. ‘Taiwanica’ orange rootstock had an intermediate behaviour, but could be distinguished from ‘Troyer’ citrange based on fluorescence parameters, since there was a variation in the basal fluorescence in the former, whereas in ‘Troyer’ citrange the basal fluorescence was not affected by the supply of Fe.  相似文献   

7.
《Scientia Horticulturae》2005,103(3):289-303
In a 2-year field study, strawberry cvs. ‘Elsanta’ and ‘Korona’ were exposed to three different levels of NaCl salinity supplied as aqueous solutions characterised by electrical conductivities of 0.3 dS/m, 2.6 dS/m, and 5.1 dS/m. Salinity in the rhizosphere reduced plant growth by up to 44% in ‘Korona’ and 90% in ‘Elsanta’. A rather distinct cultivar difference represented the reduction in leaf area per plant of 85% in the second year of experiment in ‘Elsanta’ compared to 29% in ‘Korona’. Strawberry can be regarded as a Na+ excluder, because Na+ content of both strawberry cultivars remained below 3 mg g−1 dry mass at all salinity levels. Cl content increased considerably, up to 70 mg g−1 dry mass in ‘Korona’ and 80 mg g−1 dry mass in ‘Elsanta’ plants. ‘Korona’ retained most of its Cl in roots and crowns, whereas in ‘Elsanta’ the maximum was detected in petioles. ‘Korona’ was able to accumulate up to 33% higher Cl content in the roots than ‘Elsanta’. Macronutrient deficiency due to NaCl salinity was not observed and in comparison to ‘Elsanta’, higher Cl content in roots of ‘Korona’ did not coincide with an impairment of macronutrient uptake. Salinity stress reduced fruit yield by up to 27% in ‘Korona’ and 64% in ‘Elsanta’. Fruit quality, characterised as taste, aroma, and texture by a consumer-type panel, decreased by more than 24% in ‘Elsanta’, but in ‘Korona’ differences were insignificant. Total soluble solids (Brix) and the ratio Brix/TA (TA, titratable acid) decreased significantly by about 20% in ‘Korona’ and 35% in ‘Elsanta’. To summarise, the ability of ‘Korona’ to retain Cl in the root system more effectively than ‘Elsanta’ resulted not only in a 41% lower leaf Cl content at the highest salinity level and a better growth under NaCl stress, but also in a relatively higher fruit yield and fruit quality.  相似文献   

8.
《Scientia Horticulturae》2005,104(3):237-247
In order to initiate cell and embryo suspensions, embryogenic calluses derived on NN solid medium with 2,4-D and BA from petioles of in vitro grown plants of three interspecific grapevine hybrids were cultured in three versions of liquid NN medium: (1) without growth regulators, (2) 0.1 mg l−1 IAA and (3) 0.5 mg l−1 BA. Cell and embryo suspensions were incubated two and three times in these versions of liquid media in various combinations. Incubating suspensions two times in hormone-free media and/or with 0.1 mg l−1 IAA led to formation of globular embryos in the three cultivars studied and small numbers of heart-stage embryos in ‘Podarok Magaracha’ and ‘Intervitis Magaracha’. Numerous heart-stage embryos developed in ‘Intervitis Magaracha’ and ‘Podarok Magaracha’ when the suspensions had been initiated in medium with 0.5 mg l−1 BA, and in ‘Bianca’ this was achieved after two incubations in the above medium. Torpedo-stage embryos were formed after subculturing heart-stage embryo suspensions in medium with 0.1 mg l−1 IAA in all study cultivars. If only small numbers of embryos of a certain developmental heart- or torpedo- stage were formed, such cell and embryo suspensions need to be repeatedly subcultured in liquid medium with specific growth regulators to enable this process.  相似文献   

9.
《Scientia Horticulturae》2005,106(2):203-212
Cultivation of roses in various soil-less media was studied with the aim to identify the optimum soil condition for rose production. Madelon roses grafted on rootstock of Rosa indica var. major were transplanted to polyethylene bags containing zeolite and perlite (at ratios of 25z:75p, 50z:50p, 75z:25p and 100z:0p, v/v) in a climate-controlled greenhouse. Net photosynthesis (Anet), stomatal conductance (gs) and water use efficiency (WUE) of roses were followed for 5 months. Flower production and quality were recorded in three flowering flushes during a 5-month period. Analysis of variance of repeated measurements showed that even though the overall Anet did not differ among treatments (average 18.7 μmol m−2 s−1), trends in Anet seasonality for roses in 25z:75p substrate differed significantly from those in 50z:50p, 75z:25p or 100z:0p. Stomatal conductance did not show any significant seasonality or trends in response to substrate mixtures, averaging 0.89 mol m−2 s−1. Water use efficiency was significantly lower for roses in 25z:75p than in 100z:0p mixtures (1.8 ± 0.15 and 2.0 ± 0.13 μmol m−2 s−1 CO2/mmol m−2 s−1 H2O, respectively). Cumulative production of rose plants did not differ among substrate mixtures. Productivity significantly differed among flower stem classes. Stem class I (>70 cm) and class V (≤30 cm) exhibited the least production, contributing to only 7.6 and 3.7% of the total production, respectively. The highest productivity was observed in classes III (51–60 cm) and IV (31–50 cm), contributing to the bulk of productivity (68.4%). Class II contributed a 20.3% of the production. Results showed that zeolite and perlite acted as inert materials. Zeolite did not exert any positive effect on productivity, in contrast to what has been reported in literature recently. Use of perlite resulted in a little improvement in photosynthesis, however this improvement was not reflected by a significant increase in production.  相似文献   

10.
《Scientia Horticulturae》2001,87(1-2):93-105
Campanula carpatica Jacq. ‘Blue Clips’ plants were grown in a greenhouse under nine combinations of day and night temperatures created by moving plants every 12 h among three day/night temperatures (15, 20, and 25°C). At each temperature, there were three daily light integrals (DLI; 4.2, 10.8, and 15.8 mol m−2 per day, averaged over the experimental period) created with varying supplemental light, and ambient (≈400 μmol mol−1) and enriched (≈600 μmol mol−1) CO2 concentrations. Time to flower was closely related to average daily temperature (ADT), and was not significantly affected by the day or night temperatures delivered to achieve a specific ADT. Time to flower was not largely affected by DLI or CO2 enrichment. As plant ADT increased between 15 and 25°C, flower diameter decreased about 1 mm per degree and was not related to the difference between day and night temperatures (DIF). Flower diameter was smallest and least sensitive to changes in temperature at lower DLI and at ambient CO2 levels. There were 10 less flower buds and 0.3 g less dry mass per plant at first flower for every 1° increase in plant ADT at high and medium DLIs. Flower bud number and dry mass were relatively low and less sensitive to changes in ADT at low DLI, and increased slightly with CO2 enrichment at medium and high but not at low DLI. Plant height was not related to ADT, but increased linearly as DIF increased from −6 to 12°C at all DLIs, but the response was stronger under low DLI than high and medium DLIs. Flower bud number and dry mass were correlated closely with the ratio of DLI to daily thermal time (base temperature of 0°C). Flower bud number and dry mass were highest when C. carpatica plants were grown at 15°C with a DLI of 10–15 mol m−2 per day.  相似文献   

11.
Urban parks form the largest proportion of public green spaces contributing to both physical and mental well-being of people living in urban areas. CO2 sequestration capability of the vegetation developing in parks of four historical residences (Villa Pamphjli, Villa Ada Savoia, Villa Borghese and Villa Torlonia) in Rome and its economic value were analyzed. Villa Pamphjli and Villa Ada Savoia having a larger vegetation extension (165.04 ha and 134.33 ha, respectively), also had a larger total yearly CO2 sequestration per hectare (CS) (780 MgCO2 ha−1 year−1 and 998 MgCO2 ha−1 year−1, respectively) than Villa Borghese (664 MgCO2 ha−1 year−1) and Villa Torlonia (755 MgCO2 ha−1 year−1), which had a lower vegetation extension (56.72 ha and 9.70 ha, respectively). CS was significantly correlated with leaf area index (LAI). The calculated CS for the four parks (3197 MgCO2 ha−1 year−1), corresponding to 3.6% of the total greenhouse gas emissions of Rome for 2010, resulted in an annual economic value of $ 23537 /ha.  相似文献   

12.
《Scientia Horticulturae》2004,99(2):187-197
Differences in ethylene sensitivity among carnation (Dianthus caryophyllus L.) cultivars were evaluated using a time-lapse video recording system. Measurement of time to petal inrolling of ‘White Sim’, ‘Nora’, ‘Chinera’, and line 64-54 flowers subjected to a range of 1–20 μl l−1 ethylene showed that 10 μl l−1 was the optimum concentration for sensitivity evaluation with our video system. With this system we found clear differences in ethylene sensitivity among 10 cultivars and one line. ‘Candy’, ‘Pallas’, ‘Chinera’, and line 64-54 had lower ethylene sensitivities than the other seven cultivars. Line 64-54 had the longest ethylene response time (20.6 h to start of petal inrolling). Video monitoring is a simple and accurate way of evaluating ethylene sensitivity. We have also used the system to study changes in the ethylene sensitivity of carnation flowers after anthesis. We were able to detect a shift in responsiveness to ethylene that was impossible to detect by previous methods. In the Sim-type carnation cultivars tested (‘White Sim’, ‘Scania’, ‘U Conn Sim’, and ‘Nora’), ethylene sensitivity after anthesis decreased significantly with age in both early-cut and late-cut flowers. These results clearly showed that decline of ethylene sensitivity is caused by the increasing physiological age of flowers. Ethylene sensitivity after anthesis decreased with age in normal Sim-type carnations in the same way as in long-vase-life variants such as ‘Sandrosa’.  相似文献   

13.
《Scientia Horticulturae》2005,104(3):325-337
The influence of 24 h mean air temperature (18.3, 20.6, 23.9 and 25.8 °C) and photosynthetic photon flux (PPF; 0.6, 2.1, 3.7 and 4.7 mol m−2 d−1) on the growth cycles of vegetative growth in Ilex × meserveae (‘Blue Princess’ S.Y. Hu) was investigated. Plants propagated from top cuttings were grown in greenhouse compartments. The number of unfolded leaves was recorded continuously throughout the experiment. A modified sine function was fitted to collected data and the values for the amplitude and frequency of the growth curves were analysed. The sine function was tested as a method to evaluate the influence of climate on periodically flushing species. Both amplitude and frequency were significantly influenced by air temperature and PPF. The highest frequency of flushing was found at 23.9 °C and 3.7 mol m−2 d−1. The function resulted generally in a good fit to collected data with R2 values above 0.9. Growth curves of all individual plants were categorised with respect to their growth pattern as poor synchronisation within the treatments did not allow analysis of the mean values of the growth curves.  相似文献   

14.
《Scientia Horticulturae》2005,104(3):275-292
Forty-one herbaceous species were grown under short-days (8 h photoperiod, ambient irradiance averaged 12–13.2 and 6.4–8.3 mol m−2 day−1 for Experiments I and II, respectively) with or without supplemental high-pressure sodium lighting (+50, 100, or 150 μmol m−2 s−1); or under long-days delivered using natural day lengths and irradiance with night interruption lighting (2200–0200 h at 2 μmol m−2 s−1 from incandescent lamps) or under ambient daylight plus supplemental irradiance during the day and as a day extension to 18 h (0800–0200 h) with supplemental high pressure sodium lighting (+50, 100, or 150 μmol m−2 s−1) to identify the impact of photoperiod and irradiance on flowering of each species. Days to first open flower, leaf number below first flower, and mean dry weight gain per day (MDWG) were measured when the first flower opened. Twenty-seven species were photoperiodic with examples of five photoperiodic response groups represented: obligate short-day (2), facultative short-day (5), obligate long-day (16), facultative long-day (4); 13 were day neutral (no photoperiod response in flowering). One species, Salvia sclarea L., did not flower. A facultative irradiance response was observed with 10 species; 28 species were irradiance indifferent; 2 had delayed flowering as irradiance increased. Photoperiod affected MDWG of 30 species. Increasing irradiance affected MDWG with 14 species. Photoperiod interacted with irradiance to affect MDWG of 11 species. Cobaea scandens had the greatest MDWG (0.40 g day−1) while Amaranthus hybridus had the least MDWG (0.01 g day−1) across photoperiod and irradiance levels.  相似文献   

15.
Barbados cherry fruit shows a biphasic pattern of growth whether monitored on a diameter or weight basis. Each growth phase was of about 2 weeks duration with weight gains of equal magnitude but with most of the size increase occurring in the first phase. Pyrene growth paralleled that of the whole fruit. Cell division was limited to the first week of fruit development and horticultural maturity was reached when fruit showed colour break (18 days post-anthesis). Barbados cherry is climacteric with a very high peak respiratory rate (900 ml CO2 kg−1 h−1) but a low rate of peak ethylene production (3 μl C2H4 kg−1 h−1), the former consistent with its high perishability.  相似文献   

16.
《Scientia Horticulturae》2005,106(2):190-202
The response of petunia (Petunia x hybrida Vilm.-Andr. ‘Countdown Burgundy’) and impatiens (Impatiens wallerana Hook ‘Accent Orange Tempo’) to Prohexadione-calcium was evaluated under a clear and a far-red light absorbing greenhouse (AFR) film to investigate the dosage effect of Prohexadione-Ca and to determine if it can overcome the flowering delay under FR deficient greenhouse environments. Prohexadione-Ca reduced stem elongation of petunia and impatiens under AFR and clear films when applied 3 weeks after germination. Late applications were less effective. In both crops, main stem length decreased in a quadratic pattern as the concentration of Prohexadione-Ca increased. Under both films, 50–100 mg l−1 Prohexadione-Ca resulted in ≈30% shorter petunia plants. Greater concentrations (500 and 1000 mg l−1) resulted in excessively short plants (over 70%). Prohexadione-Ca at 100 mg l−1 delayed anthesis of petunia by 8 and 3 days under the clear film and the AFR film, respectively during less inductive photoperiods but had no effect during inductive photoperiods. In impatiens, Prohexadione-Ca at 100 mg l−1 delayed anthesis over 10 days under clear or AFR film. Greater concentrations (200 and 300 mg l−1) inhibited flowering of impatiens. Prohexadione-Ca treatments significantly affected flower color development. Untreated petunia plants had dark burgundy flowers. Prohexadione-Ca treatment increased L*, a*, and C* values and decreased hue angle indicating that the flowers were faded. Flowers of untreated impatiens plants were bright orange color. Prohexadione-Ca at 100 mg l−1 increased L* value and decreased a*, b*, and C* values indicating that significant petal fading had occurred. Flowers of treated plants were nearly white under both films. Although effective in height control, loss of color would be a major limitation to the use of Prohexadione-Ca on flowering crops.  相似文献   

17.
《Scientia Horticulturae》2001,88(2):107-120
Methyl jasmonate (MJ) and CMN-pyrazole alone and in combination were applied to ‘Hamlin’ and ‘Valencia’ orange trees (Citrus sinensis (L.) Osbeck) in seven separate experiments in the 1998/1999 and 1999/2000 growing seasons to induce abscission of mature fruit for mechanical harvesting. MJ alone significantly reduced fruit detachment force (FDF) of ‘Hamlin’ oranges at concentrations of 10 mM and higher. However, MJ at 20 and 100 mM caused significant defoliation. For ‘Hamlin’ oranges, the most effective treatments were those combining 10 mM MJ and 50 mg l−1 CMN-pyrazole, and 20 mM MJ and 25 mg l−1 CMN-pyrazole. ‘Valencia’ oranges did not respond to 10 mM MJ treatments alone or in combination with CMN-pyrazole, and required MJ at 20 mM in combination with CMN-pyrazole to loosen this late-maturing variety to below 50 N, but excessive defoliation occurred.  相似文献   

18.
《Scientia Horticulturae》2001,88(4):277-287
In vitro induction of tetraploid ginger (Zingiber officinale Roscoe) and its pollen fertility and germinability were investigated. The growth of shoot tip cultures on agar MS medium containing 2.0 mg l−1 BA was greater than that of similar cultures in liquid MS medium with the same BA concentration. In liquid medium, the combinations of 0.5, 1.0, or 2.0 mg l−1 BA with 0.05 mg l−1 NAA tended to enhance the growth of the cultures. The efficiency of tetraploid induction was assessed by treating shoot tip explants on agar or in liquid MS medium containing 2.0 mg l−1 BA, 0.05 mg l−1 NAA, and 0.2% (w/v) colchicine for 4, 8, 12, and 14 days. The total number of tetraploids induced on solid medium was 18, but only five in liquid medium. On both media, the colchicine treatment for 8 days gave the maximum level of tetraploid induction. Therefore, it was found that the treatment of shoot tip explants on agar medium containing 2.0 mg l−1 BA, 0.05 mg l−1 NAA, and 0.2% (w/v) colchicine for 8 days was the most efficient way of inducing tetraploid ginger. Induced tetraploid strains, ‘4× Kintoki’, ‘4× Sanshu’, and ‘4× Philippine cebu 1’, had higher pollen fertility and germinability than the diploid counterparts, i.e., in the diploid strains, pollen fertility ranged from 0.3 to 6.2% and the germination rate from 0.0 to 0.1%, while in the tetraploid strains, pollen fertility ranged from 27.4 to 74.2% and the germination rate from 4.8 to 12.9%.  相似文献   

19.
《Scientia Horticulturae》2005,106(2):247-257
Changes in the levels of chlorophyll a and b and their derivatives, and chlorophyllase activities were compared in the pericarp of ‘Feizixiao’ (slow-degreening) and ‘Nuomici’ (fast-degreening) litchis (Litchi chinensis Sonn.). Cluster bagging and growth regulators were used to regulate the degreening process of ‘Feizixiao’. HPLC analysis revealed the presence of chlorophyllide a, pheophorbide a and pheophytin a as chlorophyll derivatives in litchi pericarp. Loss of chlorophylls in ‘Feizixiao’ pericarp was slower than that in ‘Nuomici’ pericarp. The concentrations of chlorophyll a and b in the pericarp of ‘Feizixiao’ were significantly higher than those in ‘Nuomici’ during fruit maturation, whereas the chlorophyll derivatives in the former were noticeably lower than in the latter. Chlorophyllase activity increased as chlorophylls diminished. Chlorophyllase activity peaked around the colour-break in ‘Nuomici’, being much higher than that in ‘Feizixiao’. Both cluster bagging and treatment with abscisic acid (ABA, 200 mg l−1) or jasmonic acid (50 mg l−1) significantly decreased the chlorophyll content and increased the chlorophyllase activity and the anthocyanin level. In fruit treated with 6-benzyl aminopurine (6-BA, 100 mg l−1) the degradation of chlorophylls was delayed, with concomitant decrease of chlorophyllase activity and anthocyanin level. These results imply that chlorophyllase is the crucial factor in the regulation of the chlorophyll loss in the pericarp.  相似文献   

20.
Increasing use of recycled water that is often high in salinity warrants further examination of irrigation practices for turfgrass health and salinity management. A study was conducted during 2011–2012 in Riverside, CA to evaluate the response of perennial ryegrass (Lolium perenne L.) ‘SR 4550’ turf to varying quality and quantity of irrigation water. A modified line-source sprinkler irrigation system provided a salinity gradient (ECw ∼0.6–4.2 dS m−1) in between lines. Irrigation was scheduled in four separate irrigation zones perpendicular to the irrigation lines according to 80, 100, 120, and 140% ETo. Changes in turf quality (R2 = 0.30***), were primarily driven by the number of days that the area had been irrigated with saline water. When data were separated by irrigation amount, both time and water quality accounted for 54% and 46% of the variability (P < 0.001) in quality and cover, respectively at 80% ETo. A model was created to quantify decline in turf quality in relationship to %ETo replacement and salinity accumulation in the rootzone (R2 = 0.57). Our results suggest that perennial ryegrass requires irrigation scheduling at 140% ETo, irrigation water quality below ECw ∼1.7 dS m−1, and ECe below 3.8 dS m−1 to maintain acceptable quality for 442 d in Riverside, CA.  相似文献   

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