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1.
Two monoclonal antibodies (CC17, CC29) recognizing an antigen (Bo5) on bovine T lymphocytes, analogous to human CD5 总被引：1，自引：0，他引：1
C J Howard K R Parsons B V Jones P Sopp D H Pocock 《Veterinary immunology and immunopathology》1988,19(2):127-139
Two new monoclonal antibodies (CC17 and CC29) raised against bovine thymocytes are described. The antibodies, both of which were IgG1, recognize a molecule of approximately 67,000 molecular weight on bovine T cells. They react T cells in peripheral blood, the lymph node paracortex and the periateriolar lymphoid sheath in the spleen. Both the cortex and medulla of the thymus are stained but the medulla reacts more intensely. They do not stain B cells in peripheral blood, the ileal Peyer's patch, the cortex or the primary follicles in lymph nodes. No activity was found on cells outside the lymphoid system, i.e. monocytes, alveolar macrophages or endothelial and epithelial tissue. The antigen recognized is considered to be the bovine homologue of CD5 (T1) in humans and Lyt1 in mice. The mAbs appear to be particularly useful for detecting cells in the peripheral blood of young calves which are of the T cell lineage but do not express BoT2 or the mature pan T cell antigen recognized by mAb IL-A27 and may thus allow identification of a population of bovine lymphocytes previously described as null cells. 相似文献
2.
Lymphoid organs in sturgeons (Acipenseridae) 总被引：1，自引：0，他引：1
R F?nge 《Veterinary immunology and immunopathology》1986,12(1-4):153-161
Lymphoid (lymphomyeloid) tissues in sturgeons (hybrid sturgeon, Huso huso X Acipenser ruthenus, and white Pacific sturgeon, A. transmontanus) were investigated by dissection, histology and transmission electron microscopy. The main lymphomyeloid tissues are the thymus, the spleen, the anterior part of the kidney, the meningeal myeloid tissue, the pericardial tissue and lymphoid masses of the intestine, especially in the spiral valve. The kidney is the main hemopoietic tissue. The meningeal tissue is bone marrow-like (myeloid), mainly granulopoietic, but it also contains lymphoid elements. The pericardial tissue is predominantly lymphoid. The pericardial tissue has a lymph node-like appearance. It seems to be the site of interaction between lymphocytes and vascular endothelium. The thymus contains cortex and medulla. The spleen, as in higher vertebrates, is differentiated into white and red pulp. The highly diversiform and well developed lymphoid tissues of sturgeons may serve as basis of efficient immune mechanisms. 相似文献
3.
Neurotrophin receptor-like proteins in the bovine (Bos taurus) lymphoid organs, with special reference to thymus and spleen 总被引：1，自引：0，他引：1
Levanti MB Germanà A Catania S Germanà GP Gauna-Añasco L Vega JA Ciriaco E 《Anatomia, histologia, embryologia》2001,30(4):193-198
Increasing evidence suggests that neurotrophins could regulate immune functions acting directly or indirectly on immunocompetent cells. The indirect pathway involves stromal cells of the primary and secondary lymphoid organs. In the present study the occurrence of Trk proteins (TrkA, TrkB and TrkC), regarded as the high-affinity signal-transducing receptors for neurotrophins, was investigated in cow lymphoid organs using immunohistochemistry. The thymus and spleen of both fetal and adult animals, and the palatine tonsils, lymph nodes and Peyer's patches of adult animals, were analysed. Unidentified cells displaying TrkA-like immunoreactivity were found in the fetal thymus, whereas those expressing this protein in the adult gland were identified as epithelial cells. In the spleen, immunoreactive TrkA was observed in cells of the white pulp. TrkB immunoreactivity in both fetal and adult thymus and spleen was localized in monocyte/macrophage cells. As a rule, TrkC was absent from the thymus and the spleen independent of the animal's age. Different types of stromal cells, but never the lymphocytes themselves, displayed TrkA, TrkB, or TrkC immunoreactivity in the other lymphoid organs analysed. As in other vertebrate species, Trk proteins in the lymphoid organs of the cow were localized in the stromal, non-lymphoid cells, thus suggesting that neurotrophins might regulate the immune function acting indirectly on lymphocytes. 相似文献
4.
We studied apoptosis induced by duck enteritis virus (DEV) in vivo, focusing on the lymphoid organs that constitute the main targets for infection: thymus, bursa of Fabricius (BF), and spleen. Fifty Pekin ducks were inoculated subcutaneously with a virulent strain of DEV. The morphology of lymphoid organs of these infected ducks was observed by light microscopy and transmission electron microscopy. Cell death by classical necrosis was observed in lymphocytes of the DEV-infected thymus, BF, and spleen. Lymphocyte apoptosis also was observed at the same time, and it was further confirmed by in situ terminal deoxynucleotidyl transferase dUTP nick-end labeling and agarose gel electrophoresis. We conclude that apoptosis and necrosis of lymphocytes induced by DEV infection resulted in the depletion of lymphocytes and that apoptosis of lymphocytes may play an important role in the pathogenesis of duck viral enteritis. 相似文献
5.
6.
为研究日粮中不同铁水平对绵羊免疫功能的影响，探究绵羊饲养过程中铁过量的风险，试验在绵羊基础日粮中分别添加铁500、1 000、1 500 mg/kg（硫酸亚铁形式），饲养75 d后宰杀，采取绵羊免疫器官（脾脏、胸腺、肝门淋巴结、十二指肠淋巴结及腭扁桃体），应用传统病理学方法观察以上器官的组织结构变化、含铁血黄素分布，并测定组织铁含量的变化情况。结果显示，绵羊饲喂过量铁后，脾脏白髓占比减小，淋巴细胞数量减少、排列疏松，伴有肿大、空泡化现象，红髓内有大量含铁血黄素沉积；胸腺皮质区变薄，髓质区变厚且融合，胸腺小体数量减少、结构模糊；肝门淋巴结、十二指肠淋巴结皮质与髓质区变薄、界限模糊，皮质淋巴窦与髓窦间隙增大，淋巴小结结构模糊；腭扁桃体实质内淋巴小结数量减少，毛细血管后微静脉有充血现象。饲喂过量铁后，各免疫器官组织铁含量均升高，其中脾脏内铁含量最多，腭扁桃体内最少，铁低、中、高剂量组脾脏铁含量极显著高于对照组（P<0.01）。结果表明，饲喂过量铁后，绵羊免疫器官组织结构均遭到不同程度破坏，且随着铁添加量越多，组织结构破坏程度越明显；各免疫器官中铁蓄积量与日粮中铁添加水平呈正相关，摄入过量铁严重影响绵羊免疫功能，因此在绵羊饲养过程中，要严格遵守美国NRC（1985）饲料标准，日粮中铁水平不要超过500 mg/kg。 相似文献
7.
Sections of the spleen, thymus, lymph nodes and liver, collected from mice at various time intervals after injection with either steroid hormones only, sheep red blood cells (SRBC) only, or SRBC plus steroid hormones, were compared by histological examination. A regimen of 3 injections of 4 mg of cortisol given at 24 hourly intervals was shown to have a more severe effect than 3 injections of 1 mg given at the same times irrespective of whether SRBC were injected or not. The thymic cortex showed rapid and extensive depletion of lymphocytes very soon after corticosteroid treatment and did not recover until about the 8th day. The medulla was affected to a lesser extent. Spleens and lymph nodes showed early lymphocyte destruction, active ingestion of debris by macrophages, and germinal centres were considerably decreased in number and less clearly demarcated in corticosteroid-treated animals than in SRBC immunized controls. Spleens and lymph nodes of mice that received SRBC only exhibited the characteristic morphology of active germinal centre development associated with the immune response. Corticosteroid treatment of mice sensitized with SRBC caused an increase in neutrophilic promyelocytes in bone marrow smears to the 4th day, whereafter their numbers returned to normal. The normoblasts were decreased on the 2nd and 3rd days whereafter they increased to normal. Plasma cells were increased in SRBC injected animals in bone marrow smears. Of the effects of the sex steroids studied the most notable was a drastic effect of estradiol on the thymus; both the cortex and medulla were completely depleted of lymphocytes and could hardly be distinguished. 相似文献
8.
S Sato K Ogimoto Y Nakai 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1990,52(4):719-725
Distribution of immunoglobulin(Ig)-containing cells was investigated in calves inoculated orally with live organisms of both Bacteroides succinogenes and Selenomonas ruminantium. Pathological changes and many Ig-containing cells were observed in calves which inoculated three times at 2, 3 and 26 days of age. Follicular germinal center was increased in number and size of the lymph nodes associated with the forestomach, suggesting activation of lymph apparatus. In the associated lymph nodes, IgG-containing cells were predominant and were located in both cortex and medulla, mainly in the medullary cord, B lymphocyte areas. Only a few IgA- and IgM-containing cells were observed in the lymph nodes. Accordingly, the inoculated bacteria may stimulate IgG-containing B lymphocyte populations. A few IgG-containing cells were detected in the mucosa of the forestomach. Ig-containing cells, predominantly IgG, were observed in the mucosa of the abomasum and intestine, and in the mesenteric lymph nodes. However, number of the cells in the mesenteric lymph nodes was smaller than that of the forestomach associated lymph nodes. The results suggest that the intraorally inoculated bacteria may stimulate the maturation of IgG positive lymphocytes in the lymph nodes associated with the forestomach. 相似文献
9.
Detection of proliferating lymphocytes is useful for studying immune reactions and for the prognosis of tumours of lymphocyte origin. Markers detecting proliferating cells are lacking in the dromedary camel. This study deals with the immunohistochemical detection of the Ki-67 proliferation-associated nuclear epitope using MIB-5 in frozen sections from spleens, different lymph nodes and haemal nodes of eight camels (0.5-12 years old). Frozen sections from rat spleens were labelled in parallel with camel tissue as a positive control. Large numbers of cells expressing the Ki-67 epitope were localized in germinal centres of all lymphoid organs tested. A few cells were found in the periarterial lymphoid sheath and the red pulp of the spleen, also in the lymphatic cords of the lymph nodes and the haemal nodes. There were no obvious differences in respect to the age of the animals. The Ki-67 epitope is well expressed by proliferating cells in camel lymphoid organs. MIB-5 can be applied to identify this epitope and will be a useful marker for cell production in immune reactions. 相似文献
10.
Visceral leishmaniasis in the German shepherd dog. II. Pathology 总被引：2，自引：0，他引：2
Three German shepherd dogs were inoculated with Leishmania chagasi and three with Leishmania donovani and the infection was followed for 82 days. All infected dogs developed splenomegaly and lymphadenomegaly. In lymph nodes there was a reduction in lymphocyte population in paracortical areas, extensive proliferation of macrophages in paracortical areas and medullary cords, follicular hyperplasia, and increased numbers of plasma cells. The spleen had decreased numbers of lymphocytes in periarteriolar lymphoid sheaths, proliferation of macrophages in these regions, follicular hyperplasia, and enlargement of the red pulp with clusters of macrophages and plasma cells. The morphology of the tonsil was similar to the lymph nodes. Clusters of macrophages, often containing Leishmania spp, were present in liver, bone marrow, lung, and the intestines. The morphologic changes in lymph nodes and spleen were suggestive of a suppressed cell-mediated immunity and an active humoral immunity. The German shepherd dog may be a useful laboratory model for the study of immunopathologic changes in visceral leishmaniasis. 相似文献
11.
为探讨副猪嗜血杆菌(HPS)对免疫器官的损伤,本实验将HPS血清5型岳阳株及其茵体裂解物分别接种昆明小鼠,结果显示:HPS血清5型岳阳株及其菌体裂解物,均能够引起昆明小鼠颈淋巴结细胞凋亡和坏死.组织病理学检测可见脾脏红髓扩张、白髓萎缩、淋巴细胞衰减;胸腺病变较轻,皮质部局灶性淋巴细胞坏死和散在性细胞凋亡.此外,免疫器官损伤与剂量相关,随菌量的增大病变更为明显.HPS血清5型岳阳株及其菌体裂解物对昆明小鼠淋巴器官有明显的损伤作用. 相似文献
12.
Jeklova E Leva L Matiasovic J Kovarcik K Kudlackova H Nevorankova Z Psikal I Faldyna M 《Veterinary microbiology》2008,129(1-2):117-130
Myxoma virus (MXV) causes the systemic disease myxomatosis in the European rabbit. Despite many in vitro studies on the function of MXV immunomodulatory proteins and detailed molecular knowledge of virus, little is known about the dynamics of interaction of the virus with the integrated host-immune system during infection. In this study changes in haematological profile, changes in lymphocyte subset distribution and non-specific proliferation activity of lymphocytes from different lymphoid compartments on the 2nd, 4th, 6th, 9th and 11th day after experimental infection of rabbits with MXV strain Lausanne was characterised. The relationship between alterations of immune parameters and dynamic of virus dissemination through the body was investigated. Haematological changes included moderate leucopenia with significant lymphopenia, neutrophilia, monocytosis and eosinopenia. A decrease of T cells including CD4+ and CD8+ and increase of CD79alpha+ were observed in draining popliteal lymph node 4 days after virus inoculation. From day 6, comparable changes were seen in collateral popliteal lymph node, spleen and peripheral blood. From day 9, the mentioned lymphocyte subsets tended to reach their original state in all of these lymphocyte compartments except draining popliteal lymph node. In thymus, MXV infection affected mainly CD4+CD8+ double positive thymocytes. On the other hand, proliferation activity of lymphocytes determined by the proliferation assay with plant-derived mitogens was significantly reduced from day 4 or 6 and remained reduced until the end of experiment in all observed lymphoid organs. Presence of MXV in respective lymphoid compartments preceded changes in lymphocyte subset distribution or lymphocyte activity. 相似文献
13.
The morphologic lesions associated with naturally occurring canine parvovirus infection are described. The disease is characterized by necrosis of crypt epithelium in the small intestine and of lymphoid tissue in Peyers patches, lymph nodes, spleen and thymus. 相似文献
14.
Osamu Mikami Hiroyuki Yamaguchi Hideo Murata Yasuyuki Nakajima Shigeru Miyazaki 《Journal of veterinary science (Suw?n-si, Korea)》2010,11(2):107-113
Six 1-month-old piglets were intravenously injected with deoxynivalenol (DON) at the concentration of 1 mg/kg body weight, with three pigs each necropsied at 6 and 24 h post-injection (PI) for investigation of hepatotoxicity and immunotoxicity with special attention to apoptotic changes and cytokine mRNA expression. Histopathological examination of the DON-injected pigs revealed systemic apoptosis of lymphocytes in lymphoid tissues and hepatocytes. Apoptosis of lymphocytes and hepatocytes was confirmed by the TdT-mediated dUTP-biotin nick end-labeling (TUNEL) method and immunohistochemical staining against single-stranded DNA and cleaved caspase-3. The number of TUNEL-positive cells in the thymus and Peyer''s patches of the ileum was increased at 24 h PI compared to 6 h PI, but the peak was at 6 h PI in the liver. The mRNA expression of interleukin (IL)-1β, IL-6, IL-18, and tumor necrosis factor (TNF)-α in the spleen, thymus and mesenteric lymph nodes were determined by semi-quantitative RT-PCR, and elevated expression of IL-1β mRNA at 6 h PI and a decrease of IL-18 mRNA at 24 h PI were observed in the spleen. IL-1β and IL-6 mRNA expressions increased significantly at 6 h PI in the thymus, but TNF-α decreased at 6 h PI in the mesenteric lymph nodes. These results show the apoptosis of hepatocytes suggesting the hepatotoxic potential of DON, in addition to an immunotoxic effect on the modulation of proinflammatory cytokine genes in lymphoid organs with extensive apoptosis of lymphocytes induced by acute exposure to DON in pigs. 相似文献
15.
Histological, histochemical and immunohistochemical study of the haemal nodes of the dromedary camel
Haemal nodes are lymphoid organs found in various mammals and some birds. The structure of haemal nodes has been described in a number of species but not yet in the camel. Therefore, haemal nodes from 10 camels were studied histologically and tested for CD3, CD22, major histocompatibility complex (MHC) class II/DR, alpha-smooth muscle actin and for the demonstration of acid and alkaline phosphatases. The haemal nodes were of spherical or kidney shape with one or two hili and had a capsule and trabeculae of connective tissue and smooth muscles. The main parenchyma was composed of a cortex and a medulla. The cortex was formed from lymphoid follicles and diffuse interfollicular lymphocytes. The medulla consisted of lymphoid cords separated by medullary sinuses. The interfollicular lymphocytes and those in the medullary cord were CD3-positive. The lymphoid follicles showed CD22-positive cells. MHC class II/DR was expressed by most cells of the parenchyma. There were also subcapsular, peritrabecular and medullary blood sinuses. Afferent and efferent lymphatics and lymphatic sinuses were also found. Acid phosphatase-positive cells were localized mainly in the marginal, the interfollicular zone and in the medullary cord. Alkaline phosphatase positivity was observed in the endothelium of the sinuses and in the lymphoid follicles. The morphology of these organs in the camel allows a classification as haemolymph nodes and suggests involvement in blood and lymph filtration. 相似文献
16.
F. J. Pallarés J. Seva A. Bernabé M. A. G . ómez J. A. Navarro 《Anatomia, histologia, embryologia》1999,28(3):171-176
The distribution of B cells in the lymphoid organs of the goat was studied using a panel of 13 monoclonal antibodies (mAbs) developed against different markers for bovine B cells. Samples of mesenteric lymph nodes, jejunal and ileal Peyer's patches, duodenum, jejunum, ileum, colon, caecum and rectum were taken from four 7-month-old male Murciano-granadina goats using the avidin-biotin-peroxidase (ABC) method on frozen sections as described by Hsu et al. (1981). The mAbs against immunoglobulins (Ig) recognized a large number of cells, particularly in the light zones of the germinative centres of the lymphoid follicles, regardless of the isotype against which they were directed. However, the greatest numbers of B cells in the germinative centres and outer coronas of the lymphoid follicles of the lymph node, spleen and Peyer's patches were recognized by mAbs against the L lambda chain of Ig and against IgM. This was also the case in other locations where B cells were abundant, such as the medulla of the lymph node and the dome of the Peyer's patches. These mAbs recognized not only B lymphocytes but also plasma cells, showing an intracytoplasmatic reaction (numerous in the spleen red pulp and the intestinal lamina propria when mAbs were used against the L lambda chain of the Ig, scarce in the intestinal lamina propria when used against IgM and scarce in spleen red pulp and numerous in the intestinal lamina propria when mAbs against IgA were used). The mAbs BAQ44 A, GC65 A and GB25 A are of interest because, besides marking cells in the B areas where lymphocytes show surface Ig, they give a positive reaction in areas where there are Ig-cells (the dark zone of the germinative centre) and do not immunostain plasma cells. Thus, these mAbs recognize a surface marker which is not an Ig. 相似文献
17.
Cyclophosphamide (CY) was given IV to 5-month-old calves (ten doses; each dose of 5.0 mg/kg, 2-day intervals between doses). The effects of CY on circulating leukocytes, lymphoid tissues, and the humoral and cellular immune responses were assessed. The numbers of total leukocytes, lymphocytes, and neutrophils and platelets decreased significantly. The lymphocyte population was depleted in the cortex of the thymus and B-dependent areas of the spleen and lymph nodes. Significant decreases occurred in the frequency of the peripheral blood lymphocytes-bearing surface immunoglobulin (Ig) and in serum IgM and IgG concentrations. Primary serum antibody responses to avian erythrocytes and Brucella abortus strain 19 antigens were diminished or delayed. The blastogenic responses of peripheral blood lymphocytes to phytohemagglutinin P, concanavalin A, pokeweed mitogen, and to purified protein derivative and B abortus antigens were enhanced as was the delayed hypersensitivity reaction to the tuberculin skin test. While a diminished humoral immune response was associated with CY treatment, the cell-mediated response was potentiated. The effect of CY was transitory with most variables returning to near base line within 24 days after CY was ceased. 相似文献
18.
S J Proctor 《American journal of veterinary research》1976,37(4):427-431
White Pekin ducks were inoculated orally with duck plague virus and killed at 24-hour intervals after inoculation. Spleen, thymus, and bursa of Fabricius were collected and examined by light, fluorescent, and electron microscopy. Necrosis of lymphocytes occurred in the bursa of Fabricius, thymus, splenic periarteriolar lymphoid sheath (T lymphocytes), and splenic germinal centers (B lymphocytes). Viral nucleocapsids were present in the karyoplasm of lymphocytes, but these cells necrotized before virions were formed. Periarteriolar reticular sheath cells and sinusoidal lining cells in the spleen, epithelial cells in Hassall's corpuscle of the thymus, epithelial cells between the cortex and medulla of the follicles in the bursa of Fabricius, and macrophages in all 3 tissues contained nucleocapsids in the nuclei and virions in cytoplasmic vacuoles before necrosis occurred. 相似文献
19.
H. A. Reynolds 《Canadian journal of veterinary research》1970,34(2):155-163
The lesions which characterize viral enteritis of mink (VEM) were studied in twenty-six, ten-week-old mink which had been infected by force feeding a tissue suspension containing a Wisconsin strain of mink enteritis virus. The pathogenesis of the lesions was reconstructed from gross and histopathological changes observed in animals which were selected randomly from the group each day for necropsy during the course of the disease.
Alterations were observed in the tissues of all mink examined from post-inoculation day (PID) 4 through 13. The principal macroscopic lesions which consisted of fibrinous enteritis, enlargement and hemorrhage of the spleen and edema of mesenteric and hepatic lymph nodes were most conspicuous on PID 7 and 8. Histopathological changes including necrosis and desquamation of intestinal epithelium, depletion of mature lymphocytes in lymph nodes, thymus and spleen and loss of partly differentiated myeloid and erythroid cells from spleen and bone marrow also reached full development on PID 7 and 8. However, nuclear inclusion bodies which were presumed to be a product of the causative agent and, therefore, of diagnostic significance were most prevalent on PID 3, 4 and 5. The inclusions were observed in mucosal epithelial cells of the intestine, parenchymal cells of the liver and in lymphocyte precursor cells of the spleen, intestinal lymph nodules and masenteric and hepatic lymph nodes.
相似文献20.
Pre‐scapular, femoral and mesenteric lymph nodes from five buffalo calves and five buffalo bulls were studied using light and transmission electron microscopy. The nodes were surrounded with a thin capsule of dense connective tissue and smooth muscles. Subcapsular and trabecular lymphatic sinuses were lined with endothelial cells resting on a basement membrane. The cortex was formed by lymphoid follicles and inter‐follicular lymphocytes. Primary and secondary follicles were observed. The medulla was made up of medullary cords of lymphocytes separated by lymphatic sinuses. These sinuses were lined with a discontinuous epithelium and interestingly crossed by reticular fibres. High endothelial venules were found in the paracortical area. Several lymphocytes were observed infiltrating the wall of these venules. The lymph nodes of the Egyptian water buffalo showed a typical structure compared with the majority of mammals, with no age‐related structural variation. 相似文献