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1.
文章旨在研究体外鸡胚注射肉碱对肉鸡孵化率及出壳后49 d肉鸡生长性能和屠宰性能的影响。鸡胚在孵化第18天通过羊膜注射0.6、2.4和9.6 mg左旋肉碱/100μL稀释液,此外还有3个对照组(非注射组、穿孔组和注射稀释液组),评估肉鸡孵化率、生长期肉鸡死亡率、增重、采食量和料比以及屠宰性能指标(屠宰率、腹脂、小腿肌、大腿肌、鸡翅和胸肌占比)。结果发现:鸡胚注射左旋肉碱对受精蛋孵化率无显著影响(P>0.05),但随左旋肉碱注射量的增加,受精蛋孵化率和仔鸡出壳时间有升高趋势(P=0.08)。鸡胚注射左旋肉碱对出壳后49 d肉鸡生长性能、死亡率及屠宰性能指标均无显著影响(P>0.05)。结论:鸡胚注射0.6、2.4和9.6 mg左旋肉碱/100μL稀释液对出壳后肉鸡生长性能和屠宰性能无显著影响,但可以改善肉鸡出壳时间和受精蛋孵化率。  相似文献   

2.
为探讨胚蛋注射L-精氨酸和L-鸟氨酸对内仔鸡早期个体生长发育的影响,试验选用200个孵化18 d的艾维菌肉仔鸡活胚蛋,随机分成4个处理,每个处理5个重复,每个重复10个蛋.处理组Ⅰ不注射任何营养物质作为对照,处理组Ⅱ、Ⅲ、Ⅳ分别向羊膜腔内无菌注射L-精氨酸(0.5%)、L-鸟氨酸(0.5%)、L-精氨酸+L-鸟氨酸(0.25%+0.25%)营养液1 mL.试验结果表明:①与对照组相比,胚蛋注射L-精氨酸、L-鸟氨酸及L-精氨酸+L-鸟氨酸混合物的出壳重与蛋重的比值分别提高了21.52%、14.38%、22.60%,差异达到极显著水平(P<0.01).与对照组Ⅰ和处理组Ⅱ、Ⅲ相比,处理组Ⅳ肉仔鸡出壳后第3天的体重分别提高了5.35%、6.59%、6.53%,差异极显著(P<0.01),出壳后第7天的体重分别提高了7.94%(P<0.01)、8.60%(P<0.01)、4.88%(P<0.05),处理组Ⅱ和处理组Ⅲ与对照组Ⅰ相比第3天和第7天肉仔鸡的体重均没有显著性提高(P>0.05).②胚蛋注射L-精氨酸和L-鸟氨酸可以在一定程度上提高肉仔鸡法氏囊和脾脏的重量及指数,其中处理组Ⅳ的法氏囊重与对照组Ⅰ相比提高了27.27%,差异达到了显著水平(P<0.05),但胚蛋注射L-精氨酸和L-鸟氨酸对胸腺的影响不明显(P>0.05).③胚蛋注射L-精氨酸和L-鸟氨酸可以影响肉仔鸡出壳后第3天、第7天血浆中T3、T4的含量,其中处理组Ⅲ的T3含量与对照组Ⅰ相比提高了26.11%,差异显著(P<0.05).  相似文献   

3.
本试验旨在研究胚蛋给养(in ovo feeding)β-羟基-β-甲基丁酸(beta-hydroxy-beta-methylbutyrate,HMB)对肉仔鸡孵化率、生长性能及骨骼肌发育的影响。选用540枚商品代AA肉仔鸡受精蛋,随机分为3个组:空白组(不注射)、生理盐水组(7胚龄气室注射1mL 0.9%生理盐水)、HMB组(7胚龄气室注射1mL含0.1%HMB的生理盐水)。出壳后,每组选取72只体重相近健康公雏随机分成6个重复,每重复12只。结果表明:1)胚蛋给养对种蛋孵化率无显著性影响(P0.05);2)HMB组出壳重、2l日龄体重、0~21日龄平均日增重均显著高于其他组(P0.05);3)7日龄时,HMB组肉仔鸡胸肌率较生理盐水组提高了1.01%(P=0.019);21日龄时,HMB组胸肌率显著高于其他组(P0.05),较空白组和生理盐水组分别提高了1.11%和1.04%;4)HMB组肉仔鸡的腹脂率最低,且在21日龄时显著低于空白组(P=0.018);5)与空白组和生理盐水组相比,HMB组肉仔鸡出壳当天和4日龄肌细胞直径显著增大(P0.05)。6)出壳当天和7日龄肉仔鸡,HMB组胸肌卫星细胞有丝分裂活性指数较其他两组显著提高(P0.05);7)7日龄肉仔鸡,HMB组血浆IGF-1含量显著高于空白组(P0.05),与生理盐水组之间差异不显著(P0.05)。结果提示,胚蛋给养HMB可提高肉仔鸡出壳重,增加卫星细胞有丝分裂活性,促进肉鸡胸肌发育,加快肉鸡前期生长;注射HMB不影响种蛋孵化率和肉鸡饲料转化效率;注射生理盐水与不注射处理结果相似,对肉仔鸡生长没有影响。  相似文献   

4.
本试验旨在研究胚蛋注射L-精氨酸(L-Arg)对蛋雏鸡孵化性能、1~14日龄肠道发育和血清生化指标的影响。选取47周龄京红1号蛋鸡种蛋1080枚,随机分为3组,分别为未注射对照(NC)组、生理盐水注射对照(SC)组和L-Arg注射(Arg)组,每组8个重复,每个重复45枚种蛋。在孵化第17.5天时,Arg组每枚蛋注射0.1 mL 10%的L-Arg溶液(0.85%生理盐水作溶剂,10 mg L-Arg/枚蛋),SC组注射相同剂量的0.85%生理盐水。出壳后,每组选取体重相近的健康母雏120只,随机分为8个重复。试验期14 d。结果表明:1)与NC和SC组相比,胚蛋注射L-Arg对1日龄蛋雏鸡的孵化率和出孵重无显著影响(P>0.05),但显著降低卵黄囊重(P<0.05)。2)与NC和SC组相比,胚蛋注射L-Arg显著提高1日龄蛋雏鸡的空肠长度、3日龄时的十二指肠长度和14日龄时的空肠和回肠长度(P<0.05),对3日龄蛋雏鸡空肠内容物脂肪酶活性有增加趋势(P=0.084)。3)与NC组相比,胚蛋注射L-Arg显著提高14日龄蛋雏鸡的空肠上皮细胞增殖指数(P<0.05)。4)与NC组相比,胚蛋注射L-Arg显著降低3和14日龄蛋雏鸡的血清甘油三酯(TG)含量(P<0.05),显著增加3和14日龄时的血清总胆固醇(TC)和低密度脂蛋白(LDL)含量(P<0.05)。与SC组相比,胚蛋注射L-Arg显著增加14日龄蛋雏鸡的血清高密度脂蛋白(HDL)和葡萄糖(GLU)含量(P<0.05)。由此可见,胚蛋注射10 mg L-Arg对蛋雏鸡的孵化率和出孵重等孵化性能无不良影响;胚蛋注射10 mg L-Arg促进蛋雏鸡的脂质代谢和糖代谢,为早期肠道发育提供更多能量,提高空肠上皮细胞增殖指数,增加肠道长度,促进早期肠道发育。  相似文献   

5.
蛋重对肉杂鸡种蛋孵化效果的影响   总被引:3,自引:2,他引:3  
将肉杂种蛋按蛋重大小分为7组,在相同条件下孵化。结果表明,蛋重对受精率和早期死胚率无显著影响(P>0.05),Ⅵ、Ⅶ组中后期死胚率显著升高(P<0.05);孵化至18天,Ⅰ组的相对失重显著高于其他各组(P<0.05),Ⅱ、Ⅲ、Ⅳ、Ⅴ、Ⅵ组的相对失重差异不显著(P>0.05),Ⅶ组的相对失重显著低于其它各组(P<0.05);蛋重为58.1g~66.0g时,受精蛋孵化率和入孵蛋孵化率最高,分别为92.41%~97.16%和88.00%~91.33%;雏鸡初生重随蛋重的增加而显著增大,呈强正相关(r=0.98)。  相似文献   

6.
本试验旨在研究胚蛋给养N-乙酰-L-谷氨酸(NAG)对拉萨白鸡种蛋孵化性能以及鸡胚生长、肠道形态的影响。选取17.0胚龄拉萨白鸡活胚蛋240枚,随机分为对照组和NAG给养组,每个组6个重复,每个重复20枚活胚蛋。17.5胚龄时,NAG给养组经羊膜腔无菌注射1.5%NAG营养液,注射剂量为0.1 mL,即NAG补给量为1.5 mg/枚;对照组种蛋不做处理。在19.5胚龄观测鸡胚生长指标、肠道形态,出壳当天统计孵化性能。结果表明:1)孵化后期胚蛋给养NAG对拉萨白鸡鸡胚生长无显著影响(P0.05)。2) NAG给养组种蛋孵化率显著高于对照组(P0.05),健雏率2组间无显著差异(P0.05)。3)与对照组相比,胚蛋给养NAG显著降低了19.5胚龄鸡胚十二指肠火箭形绒毛比例(P0.05),对空肠和回肠各类型绒毛比例无显著影响(P0.05)。4)与对照组相比,胚蛋给养NAG显著提高了19.5胚龄鸡胚回肠手指形绒毛高度(P0.05),对回肠手指形绒毛宽度无显著影响(P0.05);NAG给养组19.5胚龄鸡胚十二指肠、空肠手指形绒毛高度和宽度较对照组无显著差异(P0.05)。由此可见,孵化后期(17.5胚龄)经由羊膜腔给养1.5 mg/枚NAG可提高拉萨白鸡种蛋的孵化率,对鸡胚生长未产生显著影响,但可促进鸡胚肠道发育。  相似文献   

7.
日本和美国科技人员研究了孵化卵内注射氨基酸对胚胎发育及孵出鸡雏体重的影响。在孵化 7天时,用注射器向卵黄囊注射 0.5 mL氨基酸溶液,溶液中含有与卵内相同的 18种氨基酸,注射后即用石蜡封住针孔,并将卵送回孵化器中继续孵化。试验结果表明,在孵化 7天时间向卵黄囊中注射氨基酸溶液对孵化率没有影响,但是,相对于孵化前蛋重的雏鸡体重显著提高。出现这种情况,可能是由于注射增加了卵黄中氨基酸的含量或者是提高了鸡胚对氨基酸的利用。 卵内注射氨基酸提高雏鸡体重@李凯年  相似文献   

8.
本试验旨在研究胚蛋注射蛋氨酸(Met)对朗德鹅生产性能和血液生化指标的影响。将300枚种蛋分为2组,每组3个重复,每个重复50枚种蛋。在孵化第24天进行蛋氨酸胚蛋注射,对照组不进行任何处理,处理组注射Met 5 g/L,Na Cl 7.5 g/L的营养液1.5 m L。出雏后,每个重复选取体重均匀和健康的雏鹅20只进行饲养试验,饲养期28 d。结果表明:处理组对孵化率无显著影响(P0.10),但显著提高朗德鹅初生重和1~14日龄平均日增重(P0.05),同时显著降低了饲料增重比(P0.05);处理组对卵黄囊和肝脏指数无显著影响(P0.10),但显著提高了雏鹅第28天胸肌指数(P0.05);处理组显著提高了雏鹅第14天血清中总蛋白的含量(P0.05),同时有提高血清中白蛋白含量的趋势(0.05≤P0.10);在第28天,处理组有提高雏鹅血清中总蛋白和白蛋白的趋势(0.05≤P0.10);处理组显著降低了雏鹅第14和28天血清中尿酸的含量(P0.05)。试验结果显示,在孵化后期种蛋注射蛋氨酸可显著提高朗德鹅初生重和改善1~14日龄生产性能,其可能与鹅体内氨基酸利用和蛋白质沉积提高有关。  相似文献   

9.
本试验探索了不同蛋重对汶上芦花鸡种蛋孵化效果的影响。将挑选的324枚健康种蛋按蛋重分为3组:Ⅰ组(39.65~44.82 g),Ⅱ组(45.07~49.44 g),Ⅲ组(50.18~56.78 g),测算种蛋3个胚龄的失重和失重率,并对孵化效果和雏鸡初生重进行分析。结果显示:Ⅲ组的受精率、受精蛋孵化率、入孵蛋孵化率最高;种蛋的失重随蛋重增加而显著增大(P0.05),但失重率无显著差异(P0.05);雏鸡初生重随着蛋重的增加而显著增大(P0.05)。汶上芦花鸡蛋重区间为50.18~56.78 g时孵化效果最好。  相似文献   

10.
受精蛋和无精蛋、死胚及正常发育胚的种蛋品质差异研究   总被引:8,自引:0,他引:8  
通过对3批共1 626个海兰褐父母代种蛋进行试验,分析受精蛋和无精蛋、死胚及正常发育胚的种蛋品质差异,探讨种蛋品质对种蛋失重、死亡规律及孵化率的影响.结果表明:①在孵化期间出现2个死亡高峰时段,即第0~7天和第14~21天.②孵化期间第0~7天失重率与蛋壳的大、小端气孔数呈正相关;3个阶段的种蛋失重率相互之间有极显著的正相关,且出壳重与失重率有极显著的负相关.③孵化第0~7天死胚的种蛋(死精蛋)壳厚显著低于正常发育胚蛋,蛋壳两端气孔数少于正常发育胚蛋.④孵化第8~21天死胚种蛋蛋重显著高于群体平均蛋重,两端气孔数均多于正常发育胚蛋,蛋形指数小于正常发育胚蛋.⑤雏鸡出壳重与入孵蛋重有极显著正相关,与气孔数、蛋形指数、壳厚均无相关性.⑥受精蛋与无精蛋相比,入孵蛋重略大于无精蛋,壳厚显著地小于无精蛋,大、小端气孔数分别显著或不显著地多于无精蛋.  相似文献   

11.
选用已孵化18 d的肉仔鸡活胚蛋240枚,随机分成4个处理组,每个处理6个重复,每个重复10枚蛋.处理组Ⅰ注射1 mL 7.5 g/L生理盐水作为对照,处理组Ⅱ、Ⅲ、Ⅳ分别向鸡胚羊膜腔内注射质量浓度为5、10 mL/L和15 mL/L的谷氨酰胺营养液1 mL.结果表明,胚蛋注射谷氨酰胺对3日龄肉仔鸡的胸腺、脾脏和法氏囊...  相似文献   

12.
This study evaluated the influence of inoculation of different nutrients into eggs of 18-d-old broiler embryos. On d 18 of incubation, before transferring, eggs were injected with a solution containing either maltose, a multivitamin supplement, zinc-glycine, glutamine, a mixture containing all these elements, or sodium chloride (control). After hatching, 2,400 male broiler chicks were placed in an experimental broiler house and distributed into 60 floor pens in a completely randomized design comprising a 2 × 6 factorial arrangement of treatments (2 egg weights × 6 solutions), for a total of 12 treatments. Birds and feed consumption were measured weekly. At 42 d of age, 3 birds per replicate pen were randomly selected for processing. Birds derived from heavier eggs had greater hatchability and hatching weight. At 42 d of age, birds from heavier eggs had higher BW, carcass weight, and breast meat weight. The livability of birds derived from heavy eggs was higher at 7 and 14 d of age. The in ovo inoculation of the nutrients to 18-d-old embryos did not influence live performance or carcass traits. The technique of in ovo inoculation of certain nutrients may be used in industrial poultry production, but further studies are required to define the best solutions or mixture of nutrients to be used.  相似文献   

13.
试验旨在探讨胚蛋注射乳酸菌对蛋雏鸡孵化性能、免疫器官指数、十二指肠中SIgA以及血清中白细胞介素2(IL-2)和干扰素γ(IFN-γ)含量的影响.将300个海兰褐蛋鸡种蛋随机分成4组,每组5个重复,每个重复15个种蛋.18日胚龄时,试验Ⅰ、Ⅱ、Ⅲ组分别向羊膜腔内无菌注入0.2 mL含活菌数2×106、2×107和2×1...  相似文献   

14.
An experiment was conducted to evaluate the effects of in ovo injection of chrysin, quercetin and ascorbic acid on hatchability, somatic attributes, hepatic antioxidant status and early post‐hatch growth performance of broiler chicks. Four hundred and eighty embryonated broiler breeder eggs containing live 18‐day‐old embryos were divided into six groups of 80 eggs each. One group remained intact and served as a control group (i), whereas the other five groups were injected with the prepared injection solutions as follows: (ii) 0.05 ml distilled water; (iii) 0.05 ml distilled water containing 6 mg ascorbic acid; (iv) 0.05 ml dimethyl sulfoxide (DMSO); (v) 0.05 ml DMSO containing 4.5 mg quercetin; and (vi) 0.05 ml DMSO containing 4.5 mg chrysin. The hatchability rate, hatching weight, residual yolk sac weight, yolk sac‐free body weight, liver weight, hepatic glutathione peroxidase and total superoxide dismutase activities, as well as malondialdehyde concentrations, were not affected by the injected solutions. There were no differences between chicks hatched from the control and in ovo injected eggs in weight gain, feed intake and feed conversion ratio from 0 to 11 days of age. However, the specific contrast performed between the in ovo injected groups and intact eggs revealed that in ovo injection significantly increased hatchability rate (p = .0493). This finding also implies that our injection procedure was harmless. In conclusion, the intra‐egg injection of chrysin, quercetin or ascorbic acid at the injection rates used in this study did not have a significant effect on hatchability, somatic characteristics, early growth performance and hepatic antioxidant status of broiler chicks. However, the overall hatchability was higher in the in ovo injected eggs as compared to non‐injected ones. These findings also confirmed the harmlessness of the procedure developed for in ovo injection in this study.  相似文献   

15.
为探讨胚胎注射L-精氨酸和L-鸟氨酸对肉仔鸡早期肠道发育的影响,试验选用200枚孵化18d的艾维因肉仔鸡活胚蛋,随机分成4个处理组,每个处理组5个重复,每个重复10枚蛋。处理组I不注射任何营养物质作为对照,处理组II、III、IV分别向羊膜腔内无菌注射L-精氨酸(0.5%)、L-鸟氨酸(0.5%)、L-精氨酸+L-鸟氨酸(0.25%+0.25%)营养液1m。L结果表明:与对照组相比,其他各处理组的肉仔鸡早期肠道黏膜形态学结构都得以改善(P<0.05或P<0.01),其改善程度在不同时间(出壳后2、72h、7d)、不同肠段(十二指肠、空肠、回肠)、不同处理之间有所不同,且L-精氨酸与L-鸟氨酸的加性效应不明显。胚胎注射都可极显著提高肠道隐窝细胞增生率(P<0.01),其中以注射L-鸟氨酸效果最好。  相似文献   

16.
17.
1. A total of 1200 Cobb broiler breeder eggs were incubated under either standard conditions (C group) or high CO(2) levels (CO(2) group) during the first 10 d of incubation. The CO(2) level of the CO(2) incubator was attained gradually by a natural build-up of CO(2) due to air-tight closure of the incubator. From d 10 of incubation onwards, all eggs were incubated under standard incubation conditions. At d 18 of incubation, the eggs of both incubation groups (CO(2) and C group) were either injected with water-soluble dexamethasone (DEXA group) or with saline (0.9% NaCl; saline group) or were not injected (control). 2. Body weights, plasma hormonal concentrations (T(3), T(4) and corticosteroid) and glucose concentrations were measured regularly during the perinatal (at IP, internal pipping) and post-hatch period (at 1, 2, 4 and 6 weeks post-hatch). Additionally, hatchability and pattern of embryonic mortality were determined after hatch. 3. The results showed that high CO(2) levels during the first 10 d of incubation or dexamethasone injection at d 18 of incubation decreased embryo mortality, mainly because of a reduction in embryo malpositioning. However, a combination of a CO(2) incubation and a dexamethasone injection led to an increase in embryo mortality and therefore a decrease in hatchability. 4. Although dexamethasone injection at embryonic d 18 decreased body weight in the second week of the rearing period and CO(2) incubation increased body weight during the first 2 weeks of the rearing period, no consistent effect of both the incubation and injection treatments on body weight at slaughter age was observed.  相似文献   

18.
The purpose of this experiment was to investigate the effects of different intensities of monochromatic green light on hatching,embryo development,and early growth and development of chickens during incubation.810 New Pudong chicken eggs with similar size were randomly divided into 3 groups (n=270).The eggs were hatched in dark,low illumination (20~50 lux),or high illumination (200~300 lux) LED monochromatic green light (λ=525 nm) respectively during 1~18.5 days of incubation period,with 24 h light per day.From 18.5 days of incubation,the eggs of 3 groups were transferred to the same hatcher without light.Incubation temperature and humidity conditions were consistent among groups.The hatchability of fertile eggs,percentage of early dead embryo,body weight (BW) at birth and 10 weeks of age,serum hormones (thyroid hormone,calcitonin,growth hormone,melatonin and osteocalcin),and blood physiological and biochemical indicators (alkaline phosphatase,insulin-like growth factor and calcium of one-day-old and 3-week-old chicks) were examined.The results showed that 3 groups had similar early dead embryo number and percentage.At the 20.5 d after incubation,the hatchability of fertile eggs in the low illumination group and the high illumination group was about 2.3 times and 5.2 times of that in the dark group.At the 21.5 d after incubation,the hatchability of fertile eggs in the dark group was 82.8%.This parameter of the low illumination group and high illumination group was 6.5% and 1.9% higher than that of the dark group.The percentage of healthy chicks of dark group was 98.6%,this parameter of the low illumination group was 0.4% lower than that of dark group,while high illumination group and dark group was almost the same.The birth BW of males of the high illumination group was lower than that of the other two groups (P<0.05),but there was no difference between the low illumination group and the dark group (P>0.05).The trend of birth BW of females was basically consistent with that of males.The birth BW of female chicks in the high illumination group was lower than those of other two groups (P<0.05),and there was no difference between other two groups (P>0.05).At 10 weeks of age,there was no difference in BW between males or females of 3 groups (P<0.05).For the day-old chicks,there was no difference in blood and hormones indicators among groups (P>0.05).For the 3-week-old New Pudong roosters,the serum melatonin level(P<0.05)in the high and low illumination groups were higher than that in the dark group,and there was no difference in other indicators (P>0.05).In conclusion,compared to the dark condition,providing proper intensity of monochromatic green light during the incubation could promote the development of chicken embryo,and advance the hatching process without impairing chicks’health and following growth performance.  相似文献   

19.
In this study, the influence of a branched‐chain amino acid blend (BCAA composed of 3 l ‐leucine:1 l ‐valine:2 l ‐isoleucine) injected into the amniotic fluid was evaluated for embryonic growth, yolk‐sac (YS) utilization and development of gastrointestinal tract (GIT) and skeletal muscles of turkey embryos from day 24 of incubation (24E) to hatching, together with hatchability, poult quality and liver L* (lightness), a* (redness) and b* (yellowness) values at hatch. At day 22 of incubation, embryonated eggs (n = 240) were assigned to three treatments, that is, eggs were not injected (control, NC) or injected with 1.5 ml sterile solution with 0.9% salt (SA) or 0.2% BCAA blend (BCAAb). These solutions were injected manually into the amniotic fluid of the embryonated eggs. To determine weights and lengths (where appropriate) of the studied organs and tissues, four embryonated eggs and poults per treatment were selected at 24E and at hatch. While the BCAAb decreased the YS and embryo weight, hatchability and the liver L* value, it increased the weight and quality of poults and the weights of breast and thigh muscles at hatch. In conclusion, the in ovo feeding of the BCAA blend negatively affected hatchability but positively affected hatching weight and poult quality by improving development of skeletal muscles and by regulating energy metabolism.  相似文献   

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