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1.
Denitrification reactors have proven their functionality in commercial recirculation aquaculture systems (RAS). Nevertheless, clogging occurs due to the low hydraulic loads necessary to accomplish anoxic conditions for a successful denitrification process in RAS, which hampers the adjustment of stable working conditions within fixed bed denitrification reactors. Reactors working on the basis of activated sludge demand careful hydraulic control and/or complex configurations for sludge retention.To develop a low-maintenance denitrification reactor, an enclosed moving bed filter, driven by recirculation of the inherent, oxygen poor gas was designed. A Self cleaning Inherent gas Denitrification reactor (SID-reactor) of 0.65 m3, which offered a moving bed volume of 0.39 m3 was connected with a RAS of semi-industrial scale for pike perch (Sander lucioperca) production. This species indicates suboptimal environmental conditions (as e.g. NO3-N concentrations above approximately 68 mg l−1) by prompt reduction of the feed intake. In different experimental series, the SID-reactor was operated with denatured ethanol, methanol, acetic acid or glycerin as carbon sources and changing operational modes.Clogging was prevented by a 40 second inherent gas recirculation twice an hour, which provided continuous, maintenance free operation with marginal energy demand. With inlet (RAS) and outlet NO3-N concentrations in the range of 49 mg l−1 and 12 mg l−1, mean denitrification rates of 199 g to 235 g NO3-N per m3 moving bed volume and day were determined for all tested carbon sources. Negative effects on the feed intake of the reared pike perch were detected with all carbon sources except methanol. Changing the mode of operation to continuous circulation of the filter bed at inlet NO3-N concentrations of 26 mg l−1, the denitrification performance reached 451 g NO3-N per m3 moving bed volume and day. The SID-reactor allowed for the reduction of freshwater exchange in the pike perch RAS from 600 l to 70 l (−88%) and the sodium bicarbonate buffer from 182 g to 31 g (−83%) per kg of administered food. The easy and reliable operation of the SID-reactor could help to establish controlled denitrification as a routine purification step in RAS.  相似文献   

2.
《Aquacultural Engineering》2007,36(2):127-136
This study investigated formaldehyde removal in submerged fixed media biofilters in commercial and pilot scale recirculation aquaculture systems. Steady removal of formaldehyde (F) was observed immediately after simulated therapeutic treatment in closed systems and complete removal occurred within 1–4 days depending on water temperature. Formaldehyde removal was dependent on available biofilter surface area, and comparable rates of surface specific removal (SSR) were observed in two different systems. SSR was positively correlated to temperature (Q10 = 3.4) with estimates of 2.1 mg F/(m2 h) at 5.7 °C to 6.5 ± 0.2 mg F/(m2 h) at 14.5 °C. The estimates for SSR of formaldehyde can be used to predict actual treatment and effluent concentration with more accuracy. Furthermore, the results allow calculation on biofilter removal capacity of formaldehyde, applicable for developing biofilters ensuring sufficient formaldehyde removal in effluent water.  相似文献   

3.
To improve the removal efficiency for dissolved wastes within CycloBio (CB) fluidized sand biofilters (FSBs) in recirculating aquaculture systems, we investigated their structural design and optimization using computational fluid dynamics (CFD) modeling tools, an orthogonal test method, and experimental verification. Results showed that the effects of structural parameters on bed expansion from large to small were: cone height, cone diameter and slot width. The best combination was: cone height 60 mm, cone diameter 165 mm, and slot width 1.0 mm. The solid phase was well distributed not only in the radial direction, but also in the axial direction in the optimized CB FSB. The bed expansion (40%–120%) was increased about 13%. Energy savings were 21%–28% at the same bed expansion. When the optimized CB FSB was used to treat synthetic aquaculture wastewater, with three bed expansions and four levels of C/N, total ammonia nitrogen removal rate expressed per unit of expanded bed volume was high, from 629 to 881 g m−3 day−1. All results indicated that the structure of the optimized CB FSB was more reasonable and that the combination of CFD simulation and the orthogonal test method could be successfully applied to structural optimization.  相似文献   

4.
In zero-exchange superintensive culture systems, flocculated particles (bioflocs) accumulate in the water column. Consequently, some control over the concentration of these particles must be performed. The objective of this study is to evaluate the effects of three concentrations of bioflocs on microbial activity, selected water quality indicators and performance of Litopenaeus vannamei in a tank system operated with no water exchange. A 44-day study was conducted with juvenile (6.8 g) shrimp stocked in twelve 850 L tanks at a stocking density of 459 shrimp m−3. Biofloc levels were expressed as three presets of total suspended solids (TSS) concentrations, as follows: 200 mg L−1 (T200), 400–600 mg L−1 (T400–600), and 800–1000 mg L−1 (T800–1000). TSS levels were controlled by attaching a 40 L settling tank to each culture tank. Reduction of TSS to concentrations close to 200 mg L−1 decreased the time of bacterial cell residence and significantly reduced the nitrification rates in the water (P < 0.05). The tanks in the T200 treatment had a greater variability of ammonia and nitrite (P < 0.05), which led to the need to increase the C:N ratio of the organic substrate to control ammonia through its assimilation into heterotrophic bacterial biomass. But the higher production of heterotrophic bacteria in T200 (P < 0.05) increased the dissolved oxygen demand. Nitrification rates were higher (P < 0.05) in tanks with TSS concentrations above 400 mg L−1, and ammonia and nitrite were significantly lower than in the T200 tanks. We suggest that ammonia and nitrite in the T400–600 and T800–1000 tanks were controlled primarily by nitrifying bacteria, which provided higher stability of these parameters and of dissolved oxygen. Regarding shrimp performance, the reduction of TSS to levels close to 200 mg L−1 was associated with better nutritional quality of bioflocs. Nevertheless, differences in biofloc levels and nutritional quality were not sufficient to affect the weight gain by shrimp. The rate of shrimp survival and the final shrimp biomass were lower (P < 0.05) when the TSS concentrations were higher than 800 mg L−1. Analysis of the shrimps’ gills showed a higher degree of occlusion in the T800–1000 treatment (P < 0.05), which suggests that the shrimp have an intolerance to environments with a solids concentration above 800 mg L−1. Our results show that intermediate levels of bioflocs (TSS between 400 and 600 mg L−1) appear to be more suitable to superintensive culture of L. vannamei since they create factors propitious for maintaining the system’s productivity and stability  相似文献   

5.
Three different commercially available structural plastic media were evaluated in triplicate in moving bed biofilters under low salinity (11–12 ppt) warm water culture conditions and two different feed loading rates. The culture system consisted of nine separate modules that include a double drain fish culture tank paired to a moving bed biofilter. The biofilters were filled with 0.11 m3 of one of three different types of floating plastic structured media. The three types of media evaluated were K1 kaldnes media, MB3 media, and AMB media. Volumetric total ammonia nitrogen (TAN) removal rates (g TAN removed/m3 media-day), TAN removal efficiency, and biofilm kinetic constants, Ki (h−1) were determined for the three media types at two different daily feed load rates of 3.5 and 8.2 kg feed/m3 media. The feed provided was a 4.8 mm slow sinking marine grower diet pellet (45% protein, 17% fat). Average (±standard deviation, SD) volumetric TAN removal rates (VTR) at the lower feed load for the three media types were 92.2 ± 26.3, 86.1 ± 27.5, and 82.5 ± 25.9 for the MB3, AMB, and K1 kaldnes media, respectively. At the higher feed load the average VTR for the three media types was 186.4 ± 53.7, 172.9 ± 47.8, and 139.9 ± 38.9 for the MB3, AMB, and K1 kaldnes media, respectively. Influent TAN concentrations varied by the feed load rate and ranged from 0.55 to 0.93 mg/L and 0.83 to 1.87 mg/L for the low and higher feed loads, respectively. The percent TAN removal rates for the MB3 media was the highest of the three media types at both the low and high feed load rates averaging 12.3% and 14.4%, respectively. The MB3 media was selected for use in the moving bed biofilters because of the greater VTR and removal efficiency results for use in the 0.11 m3 moving bed biofilters of the hatchery recirculating aquaculture system.  相似文献   

6.
The ability to consistently and cost-effectively reduce nitrate-nitrogen loads in effluent from recirculating aquaculture systems would enhance the industry's environmental stewardship and allow improved facility proximity to large markets in sensitive watersheds. Heterotrophic denitrification technologies specifically employing organic carbon found in aquaculture system waste offer a unique synergy for treatment of land-based, closed-containment production outflows. For space-efficient fluidized sand biofilters to be used as such denitrification reactors, system parameters (e.g., influent dissolved oxygen and carbon to nitrogen ratios, C:N) must be evaluated to most effectively use an endogenous carbon source. The objectives of this work were to quantify nitrate removal under a range of C:Ns and to explore the biofilter bacterial community using three replicated fluidized sand biofilters (height 3.9 m, diameter 0.31 m; fluidized sand volume plus biofilm volume of 0.206 m3) operated at a hydraulic retention time of 15 min and a hydraulic loading rate of 188 L/min m2 at The Conservation Fund Freshwater Institute in Shepherdstown, West Virginia, USA. Nitrate reduction was consistently observed during the biofilter study period (26.9 ± 0.9% removal efficiency; 402 ± 14 g NO3-N/(m3 biofilter d)) although nitrite-N and total ammonium nitrogen concentrations slightly increased (11 and 13% increases, respectively). Nitrate removal efficiency was correlated with carbonaceous oxygen demand to nitrate ratios (R2 > 0.70). Nitrate removal rates during the study period were moderately negatively correlated with influent dissolved oxygen concentration indicating it may be possible the biofilter hydraulic retention time was too short to provide optimized nitrate removal. It is reasonable to assume that the efficiency of nitrate removal across the fluidized sand biofilters could be substantially increased, as long as organic carbon was not limiting, by increasing biofilter bed depths (to 6–10 m), and thus hydraulic retention time. These findings provide a low-cost yet effective technology to remove nitrate-nitrogen from effluent waters of land-based closed-containment aquaculture systems.  相似文献   

7.
Biological filtration, or biofiltration, is the key technology in recirculating aquaculture systems. Sequencing microbead biofilters, in which the media maintains a continuous up-and-down movement, are based on traditional microbead filters but offer superior filtration properties. The performance characteristics of a sequencing microbead biofilter installed in a recirculating aquaculture system for rearing Barcoo perch at 29 ± 1 °C were examined. The total ammonia-nitrogen (TAN) concentrations and the nitrite-nitrogen concentrations during a 52-day culture period were maintained blow 1.6 mg/L and 0.9 mg/L. In order to ensure efficient biofiltration, the optimal actual application of hydraulic retention time was determined to be approximately 3–5 min. The water flow produced by the reciprocating motion of the media served to wash away suspended solids, ensuring the occurrence of optimal nitrification processes. Additionally, the reciprocating motion of the media enhanced ammonia treatment efficiency significantly by improving the transport of nutrients and nitrification activity. Compared to a static situation the ammonia removal rate increased by 27% based on the application of up-and-down reciprocating movement. The biofilm on the microbead forms as a compact, complex, and homogeneous structure, consisting of numerous microscopic thin sheets. Additionally, a multitude of pores, interstitial voids, and vertical channels were widely observed to convey obviously advantageous properties in support of fluid passage, thus enhancing mass transfer and ultimately contributing to biofiltration effectiveness. The optimum biofilm thickness for providing efficient biofiltration was determined to be approximately 70 μm for this filter.  相似文献   

8.
Particulate and dissolved nitrogen (N) waste components are removed in recirculating aquaculture systems (RAS) using different cleaning technologies, and to dimension and optimize their removal efficiency requires that the expected daily load of the different waste forms can be estimated. Using a laboratory, mass-balance approach, the current study examined the effects of commercially applied feeding levels on the loading of different N waste forms, including daily fluctuations in dissolved total nitrogen (TN), total ammonia nitrogen (TAN), urea-N, and non-characterized, dissolved N deriving from juvenile rainbow trout (Oncorhynchus mykiss). In addition, the study examined whether there was a removal of urea-N across a moving bed biofilter operated as end-of-pipe under commercial conditions. The laboratory, mass-balance study showed that there were no effects of feeding levels (1.3, 1.5 or 1.7% of the biomass per day ) on the excretion of dissolved N components, which constituted the majority of total N waste (>81.6% on average). The excretion of urea-N and non-characterized, dissolved N components constituted 12–13% and 9–11%, respectively of dissolved TN. The excretion of urea-N was largely constant and independent of the daily feeding practice, whereas that of non-characterized N appeared to reflect the daily feeding activity, following the trends in TN and TAN. The time limited feeding regime applied in the laboratory study resulted in a pulse in the excretion of TAN that a biofilter may be unable to fully level out, potentially resulting in unnoticed, critical water quality conditions in intensive RAS during certain times of the day. Particulate N waste constituted a minor fraction of total N waste (<18.4% on average), and the actual loading depended on the digestibility of dietary protein/nitrogen. Results from the commercially operated, nitrifying biofilter showed that urea-N was removed at a rate of 0.014 g N m2 day−1. Compared to the removal of TAN (0.208 g N m2 day−1), the moving bed biofilter was 1.07 times more active in removing dissolved N than immediately expected when only considering TAN.  相似文献   

9.
There is a need to develop practical methods to reduce nitrate–nitrogen loads from recirculating aquaculture systems to facilitate increased food protein production simultaneously with attainment of water quality goals. The most common wastewater denitrification treatment systems utilize methanol-fueled heterotrophs, but sulfur-based autotrophic denitrification may allow a shift away from potentially expensive carbon sources. The objective of this work was to assess the nitrate-reduction potential of fluidized sulfur-based biofilters for treatment of aquaculture wastewater. Three fluidized biofilters (height 3.9 m, diameter 0.31 m; operational volume 0.206 m3) were filled with sulfur particles (0.30 mm effective particle size; static bed depth approximately 0.9 m) and operated in triplicate mode (Phase I: 37–39% expansion; 3.2–3.3 min hydraulic retention time; 860–888 L/(m2 min) hydraulic loading rate) and independently to achieve a range of hydraulic retention times (Phase II: 42–13% expansion; 3.2–4.8 min hydraulic retention time). During Phase I, despite only removing 1.57 ± 0.15 and 1.82 ± 0.32 mg NO3–N/L each pass through the biofilter, removal rates were the highest reported for sulfur-based denitrification systems (0.71 ± 0.07 and 0.80 ± 0.15 g N removed/(L bioreactor-d)). Lower than expected sulfate production and alkalinity consumption indicated some of the nitrate removal was due to heterotrophic denitrification, and thus denitrification was mixotrophic. Microbial analysis indicated the presence of Thiobacillus denitrificans, a widely known autotrophic denitrifier, in addition to several heterotrophic denitrifiers. Phase II showed that longer retention times tended to result in more nitrate removal and sulfate production, but increasing the retention time through flow rate manipulation may create fluidization challenges for these sulfur particles.  相似文献   

10.
One of the challenges that Recirculating Aquaculture Systems (RAS) are still facing is the risk that in RAS fish grow less than in flow-through systems due to the accumulation of substances originating from feed, fish or bacteria associated with the water re-use. The present study investigated whether RAS with high and low accumulation levels of these substances affect feed intake and growth of Nile tilapia Oreochromis niloticus, African catfish Clarias gariepinus, and European eel Anguilla Anguilla. One-hundred and twenty individuals of each species were used (start body weights: Nile tilapia 264.8 ± 8.3 g; African catfish 253.2 ± 2.1 g and European eel 66.6 ± 1.3 g). For a period of 39 days, growth and feed intake were compared between high and low accumulation RAS. HIGH accumulation RAS was designed for maximal accumulation of substances in the water by operating the system at nearly-closed conditions (30 L/kg feed/d), using mature biofilters and high feed loads; and (2) LOW accumulation RAS was designed to be a proxy for flow-through systems by operating at high water exchange rates (1500 L/kg feed/d), new biofilters and low feed load. HIGH accumulation RAS induced a reduction in feed intake (42%) and growth (83%) of Nile tilapia, as compared to systems that are a proxy for flow-through conditions. This effect was not observed in European eel and African catfish. The cause of this reduced feed intake and growth rate of Nile tilapia is still unclear and should be addressed in further studies.  相似文献   

11.
A new physico-chemical process for ammonia removal from fresh-water recirculated aquaculture systems (RASs) is introduced. The method is based on separating NH4+ from RAS water through an ion-exchange resin, which is subsequently regenerated by simultaneous chemical desorption and indirect electrochemical ammonia oxidation. Approach advantages include (1) only slight temperature dependence and no dependence on bacterial predators and chemical toxins; (2) no startup period is required and the system can be switched on and off at will; and (3) the fish are grown in much lower bacterial concentration, making the potential for both disease and off-flavor, lower. A small pilot scale RAS was operated for 51 d for proving the concept. The system was stocked by 105 tilapia fish (initial weight 35.8 g). The fish, which were maintained at high TAN (total ammonia nitrogen) concentrations (10–23 mgN L−1) and fish density of up to 20 kg m−3, grew at a rate identical to their established growth potential. NH3(aq) concentrations in the fish tank were maintained lower than the assumed toxicity threshold (0.1 mgN L−1) by operating the pond water at low pH (6.5–6.7). The low pH resulted in efficient CO2 air stripping, and low resultant CO2(aq) concentrations (<7 mg L−1). Due to efficient solids removal, no nitrification was observed in the fish tank and measured nitrite and nitrate concentrations were very low. The system was operated successfully, first at 10% and then at 5% daily makeup water exchange rate. The normalized operational costs, calculated based on data derived from the pilot operation, amounted to 28.7 $ cent per kg fish feed. The volume of the proposed process was calculated to be ∼13 times smaller than that of a typical RAS biofilter. The results show the process to be highly feasible from both the operational and economical standpoints.  相似文献   

12.
The removal of Tricaine Methanesulfonate from water in a fixed-bed column packed with pyrolysed paper mill sludge was evaluated for application in Recirculating Aquaculture Systems. Breakthrough adsorption curves were determined for this pharmaceutical, under different operating conditions. In a laboratory-scale approach, with synthetic effluent, a bed adsorption capacity of 125 mg g−1 was obtained under a flux of 56 L min−1 m−2, with 400 mg L−1 of MS-222 with 4.5 cm bed depth. The performance of the continuous adsorption in a column packed with biological paper mill sludge-based carbon, using real aquaculture wastewater as matrix, was not significantly affected. Then, scale-up was carried out and the bed depth service time model was applied and provided good predictions. Finally, the thermal regeneration of the exhausted adsorbent was assessed, which revealed satisfactory results for one cycle reusability after saturation; however, considering the nature of its precursor, the use of new adsorbent could be more profitable than its regeneration, functioning also as an additional option for the management of such wastes.  相似文献   

13.
From an environmental point of view, hydrogen peroxide (HP) has beneficial attributes compared with other disinfectants in terms of its ready degradation and neutral by‐products. The rapid degradation of HP can, however, cause difficulties with regard to safe and efficient water treatment when applied in different systems. In this study, we investigated the degradation kinetics of HP in biofilters from water recirculating aquaculture systems (RAS). The potential effect of HP on the nitrification process in the biofilters was also examined. Biofilter elements from two different pilot‐scale RAS were exposed to various HP treatments in batch experiments, and the HP concentration was found to follow an exponential decay. The biofilter ammonia and nitrite oxidation processes showed quick recuperation after exposure to a single dose of HP up to 30 mg L?1. An average HP concentration of 10–13 mg L?1 maintained over 3 h had a moderate inhibitory effect on the biofilter elements from one of the RAS with relatively high organic loading, while the nitrification was severely inhibited in the pilot‐scale biofilters from the other RAS with a relatively low organic loading. A pilot‐scale RAS, equipped with two biofilter units, both a moving‐bed (Biomedia) and a fixed‐bed (BIO‐BLOK®) biofilter, was subjected to an average HP concentration of ~12 mg L?1 for 3 h. The ammonium‐ and nitrite‐degrading efficiencies of both the Biomedia and the BIO‐BLOK® filters were drastically reduced. The filters had not reverted to pre‐HP exposure efficiency after 24 h, suggesting a possible long‐term impact on the biofilters.  相似文献   

14.
There is a need for simple, maintenance-free technologies for removing nitrogen (N) from aquaculture effluents. Denitrifying woodchip bioreactors have been used successfully to remove nitrate-N (NO3-N) from ground and surface waters and may potentially be applied to dilute aquaculture effluents as well. Real-life applicability in commercial, outdoor fish farms including practical start-up issues such as e.g. time till stable performance and potential leaching are, however, unknown to the industry.This case study consequently investigated the temporal performance of a woodchip bioreactor (12.5 m3) during start-up. The bioreactor was operated end-of-pipe at a commercial, outdoor rainbow trout (Oncorhynchus mykiss) farm in Denmark operated at low recirculation intensity. Applying an empty bed contact time (EBCT) of 5 h, the specific objectives of the study were to resolve: i) how fast the bioreactor would start to remove NO3-N; ii) how fast steady state was achieved; iii) which NO3-N removal rates could be attained at the relatively low effluent temperature (∼8 °C) and iv) to which extent any concomitant leaching of phosphorous (P), ammonia or organic matter would occur.In- and outlet grab samples were obtained every 6 h until the bioreactor was in steady state (2 weeks) followed by weekly 24 h pooled samples for another 3 weeks (5 weeks in total). Additional grab samples were obtained from 9 sampling ports within the bioreactor on 3 consecutive days during steady state. Samples were analyzed for dissolved nutrients (total N, nitrate, nitrite, ammonium, total phosphorous, ortho-phosphorous, BOD5 and COD). In addition, oxygen, temperature and pH were logged every 30 min while sampling and alkalinity were measured once a week.Removal of NO3-N started immediately and remained stable at 7.06 ± 0.81 g NO3-N/m3/d (n = 6) throughout the sampling period. Increased effluent NO2-N concentrations (peaking at 1.14 mg NO2-N/l after 4–5 days) were transiently observed during the initial 11 days. After that, the woodchip bioreactor was largely in steady state with respect to N-balances corroborated by a close match between filtered total-N (TNdiss) and NO3-N removal rates. Measurements within the bed showed that the majority of the influent dissolved oxygen (DO) was consumed within the first part of the bioreactor and that NO3-N removal thereafter proceeded gradually with distance within the bed. Leaching of non-structural, dissolved organic compounds were observed just after startup, causing a short-term (1 week) increase in effluent concentrations of COD, BOD5, P and ammonium.Additional measurements carried out until 147 days after start-up showed that the woodchip bioreactor continued to remove TNdiss at an average removal rate of 7.81 ± 0.82 g N/m3/d, and that the initial leakage of P stopped altogether.In summary, the study demonstrated that woodchip bioreactors can effectively remove NO3-N from dilute aquacultural effluents at low temperatures and commercial conditions and that stable performance is achieved within a few weeks.  相似文献   

15.
The hydrodynamic characterization and the performance evaluation of an aerobic three phase fluidized bed reactor in wastewater fish culture treatment are presented in this report. The objective of this study was to evaluate the organic matter, nitrogen and phosphorous removal efficiency in a physical and biological wastewater treatment system of an intensive Nile Tilapia laboratory production with recirculation. The treatment system comprised of a conventional sedimentation basin operated at a hydraulic detention time HDT of 2.94 h and an aerobic three phase airlift fluidized bed reactor AAFBR operated at an 11.9 min HDT. Granular activated carbon was used as support media with density of 1.64 g/cm3 and effective size of 0.34 mm in an 80 g/L constant concentration. Mean removal efficiencies of BOD, COD, phosphorous, total ammonia nitrogen and total nitrogen were 47%, 77%, 38%, 27% and 24%, respectively. The evaluated system proved an effective alternative for water reuse in the recirculation system capable of maintaining water quality characteristics within the recommended values for fish farming and met the Brazilian standards for final effluent discharges with exception of phosphorous values.  相似文献   

16.
A model is presented for the prediction of mercury (Hg) concentrations in the edible tissues of farmed southern bluefin tuna, Thunnus maccoyii (SBT) during commercial culture. The predictive model was developed using the initial Hg concentration of wild-caught SBT (mg kg? 1), Hg concentration of feed (mg kg? 1), SBT feeding rate (kg kg? 1 day? 1), SBT tissue growth rate (kg day? 1) and least squares minimisation estimation of Hg uptake efficiency, η. Model development and validation was based on experimental data collected from commercially cultured SBT which are farmed in in-sea pontoons (sea-pontoons). Model predictions indicate that over a typical farming season SBT from each of three commercially operated sea-pontoons experienced a 0.1 mg kg? 1, 0.14 mg kg? 1 and > 0.2 mg kg? 1 reduction in the Hg concentration of muscular tissues respectively. This is the first study to attempt to estimate Hg uptake efficiency in a tuna species. Estimation of Hg uptake efficiency and development of a working model for prediction of Hg concentration in SBT under varying culture diets, represent the first steps towards taking the guesswork out of managing Hg residues in cultured tuna.  相似文献   

17.
The removal of phytoplankton cells from aquaculture systems generally results in the reduction of nitrogenous waste and improves water quality. With this study, the effects of chitosan concentration, environmental condition and pH adjustment on flocculation of phytoplankton in marine shrimp (Litopenaeus vannamei) culture tanks were investigated. The remaining phytoplankton and suspended solids in the system were indicators for evaluating the efficiency of chitosan on flocculation. The results indicate that the flocculation efficiency of chitosan was highest (>85%) and remained fairly constant at a chitosan concentration of 40–80 mg L?1 and a pH range of 7–9 after chitosan addition. With this novel technique including 40 mg L?1 chitosan addition, pH adjustment to 6.5 and then to 8.5, high efficiency and consistency of flocculation were achieved. This technique could also be applied with various water alkalinity up to 400 mg CaCO3 L?1. The experiment for phytoplankton removal by chitosan flocculation in the recirculating aquaculture system showed that flocculation efficiency remained constant even though flocculation was repeated several times.  相似文献   

18.
《Aquacultural Engineering》2010,42(3):188-193
The removal of phytoplankton cells from aquaculture systems generally results in the reduction of nitrogenous waste and improves water quality. With this study, the effects of chitosan concentration, environmental condition and pH adjustment on flocculation of phytoplankton in marine shrimp (Litopenaeus vannamei) culture tanks were investigated. The remaining phytoplankton and suspended solids in the system were indicators for evaluating the efficiency of chitosan on flocculation. The results indicate that the flocculation efficiency of chitosan was highest (>85%) and remained fairly constant at a chitosan concentration of 40–80 mg L−1 and a pH range of 7–9 after chitosan addition. With this novel technique including 40 mg L−1 chitosan addition, pH adjustment to 6.5 and then to 8.5, high efficiency and consistency of flocculation were achieved. This technique could also be applied with various water alkalinity up to 400 mg CaCO3 L−1. The experiment for phytoplankton removal by chitosan flocculation in the recirculating aquaculture system showed that flocculation efficiency remained constant even though flocculation was repeated several times.  相似文献   

19.
Laboratory data and information from the literature were used to develop an equation that models ammonia removal by submerged biofilters. The equation is based on the half-order/zero-order kinetics model and fixed biofilm nitrification, which indicates that the nitrification rate is a linear function of the ammonia concentration at ammonia concentrations <2.0 mg/l. Input data for the equation include easily gathered information of flow rate, biofilter size, maximum permissible ammonia level, biofilter influent and biofilter effluent ammonia concentrations, temperature, and ammonia production rate. The equation can be used to determine the carrying capacity of the submerged biofilters, to estimate biofilter sizes needed to support various fish loads, and to investigate the effects of changes in biofilter system parameters on carrying capacity. Comparisons of the predicted and actual carrying capacities of several experimental biofilters indicated that the equation accurately predicted carrying capacity under most conditions. Studies of the sensitivity of the equation to changes in system parameters demonstrated that flow rate limits biofilter performance and suggested an approach for determining cost-effective flow rates for biofilter operation.  相似文献   

20.
Florfenicol (Aquaflor®) is the only U.S. Food and Drug Administration (FDA) approved drug for treating diseased fish reared in recirculating aquaculture systems (RAS). Treating diseased fish in RAS is challenging because of the potential to damage nitrifying bacteria in the biofilters. Impaired nitrification can lead to concentrations of ammonia and nitrite that compromise fish welfare. The objective of this study was to determine the effects of a FDA‐approved parasiticide and fungicide, Parasite‐S® (formalin), on biofilter nitrification. Stable biofilters were exposed once to 0, 9.25, 18.5, 37, or 55.5 mg/L formaldehyde. Total ammonia nitrogen (TAN) and nitrite nitrogen were monitored daily before and throughout the study to quantify biofilter function. Formaldehyde concentrations ≥37 mg/L increased TAN and nitrite nitrogen concentrations, and nitrification did not recover to pre‐exposure concentrations up to 8 day postexposure. On the basis of those results, a second trial was conducted. Stable biofilters were exposed once or on four consecutive days to 9.25 or 18.5 mg/L formaldehyde. Biofilters repeatedly exposed to formaldehyde showed signs of impairment and had variable recovery relative to single exposures. Results of this study may help identify formaldehyde concentrations that can be safely applied to RAS when treating diseased fish.  相似文献   

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