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在前期功能性试验基础上 ,根据商品化要求 ,将蜂胶制剂制成乳化型制剂和与蜂蜜混合型制剂等 ,以此为样品与前期制剂和市场同类制剂进行功能性比较试验。实验证明 :该乳化制剂在总黄酮含量、抗菌活性、降血糖等生物学功能上均优于或接近其他产品  相似文献   

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This article reviews the effects of using Yersinia enterocolitica serotype: 09 somatic antigen in diagnostic tests for brucellosis. The tests employed include the standard tube agglutination test, the microplate agglutination test, the quantitative plate agglutination test, the growth agglutination test and the indirect hemagglutination technique. Only the growth agglutination technique demonstrated an increased sensitivity over the STAT. Neither the microplate agglutination test nor quantitative plate tests offered any significant advantages over the STAT. The QPAT using Brucella O antigen was clearly subject to false negative results. The indirect hemagglutination technique showed extreme variation in results, the significance of which (if any) was unclear.  相似文献   

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The purpose of our study was to describe synchronous primary tumours and previously undetected metastases in 736 dogs with confirmed neoplasia in which computed tomography (CT) scans were performed for diagnostic, staging and/or radiation treatment planning purposes. All CTs were reviewed by a radiologist. Tumour‐associated CT abnormalities were detected in 38/736 (5%), including confirmed or suspected synchronous primary neoplasms (n = 24), metastases of the primary tumour (n = 9) or both (n = 3). In lymph nodes (LN) that were considered abnormal on CT scan and were aspirated, 23% contained metastasis, and 6% of ‘normal’ appearing LN that were aspirated contained metastasis. Thorough evaluation of CTs and routine aspiration of regional LN are critical because results affect recommendations to perform additional staging tests and treatment for the primary and secondary tumour(s).  相似文献   

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BACKGROUND: Alterations in the appearance and function of gastrointestinal mucosa are common after strenuous exercise. However, the duration of exercise required to alter the gastrointestinal mucosa has not been reported. HYPOTHESIS: We used 42 sled dogs to test the hypothesis that the magnitude of exercise-induced gastrointestinal mucosal dysfunction is related to exercise duration. ANIMALS: Six dogs served as conditioned controls, and the remaining dogs were randomly chosen for examination after 1-5 consecutive days of running at 100 miles/d. METHODS: Gastroduodenoscopy and measurement of gastric permeability were performed 24 hours after cessation of exercise. Intestinal protein loss (represented by fecal alpha-1 protease inhibitor concentration) was measured within 6 hours of cessation of exercise. Twelve of the 42 dogs were examined again after 5 months of detraining to determine the effect of training on gastrointestinal mucosal function. RESULTS: Exercise increased gastric permeability (P = .04) and endoscopic severity of gastric lesions (P < .0001), but neither variable was significantly affected by distance traveled. Acute exercise had no effect on intestinal protein loss. Untrained dogs had significantly lower fecal alpha-1 protease inhibitor concentrations compared with trained, unexercised dogs. Training had no effect on gastric permeability to sucrose or the endoscopic appearance of the stomach. CONCLUSIONS AND CLINICAL IMPORTANCE: These data suggest that relatively modest exercise is required to increase intestinal protein loss, but more substantial exercise is required to cause alterations in the proximal gastrointestinal tract. However, none of these alterations appear to progress with increasing exercise duration.  相似文献   

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本试验对某猪场关节肿大疑似猪链球菌的仔猪进行剖检并无菌操作抽取关节液,对3份关节液进行细菌分离和鉴定,共分离到2株病原菌,对这2株病原菌进行革兰氏染色,结果都为G球菌,多数成双,也有三五个一起,少数成长链;进一步通过生化鉴定、溶血现象观察和动物致病性试验,结果表明,其菌落形态、染色、培养特性及理化性质符合链球菌的特点,并都具有β溶血现象;通过药敏试验发现,2株菌株对许多种药物都产生了一定的耐药性,只有头孢唑啉和哌拉西林为高度敏感药物。综合该养殖场仔猪关节肿大的流行病学、临床症状、病理剖检、细菌分离、鉴定结果和临床用药效果,判定该场仔猪关节肿大的主要病原为关节炎型链球菌感染所致。  相似文献   

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OBJECTIVE: To determine the most relevant aero-allergens involved in canine atopic dermatitis in southeastern Australia and provide information about these aero-allergens to the general practitioner. PROCEDURE: Dogs presented to the Animal Skin & Allergy Clinic with history and clinical signs of atopic dermatitis were injected intradermally with 38 different allergens and negative and positive control. Intradermal skin tests in 1000 dogs were retrospectively evaluated. RESULTS: One third of all patients reacted to the house dust mite Dermatophagoides farinae. Allergens reacting in more than 15% of the patients were wheat (Triticum aestivum), sweet vernal (Anthoxanthum odoratum), English couch (Agropyron repens), yellow dock (Rumex crispus), Mexican tea (Chenopodium ambrosioides), plantain (Plantago lanceolata), melaleuca (Melaleuca quinquenervia) and peppercorn (Schimus spp). CONCLUSION: House dust mites are the most common allergens in canine atopic dermatitis in southeastern Australia and D farinae is involved most frequently. However, a number of grass, weed and tree pollens also are involved regularly.  相似文献   

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利用PALL滤器将灭活后的新城疫、禽流感、传染性支气管炎、产蛋下降综合征等病毒分别进行10倍浓缩,经检验合格后,分别按照一定比例将抗原浓缩液与灭菌的医用白油配比,经胶体磨制备成三联或四联油乳剂灭活疫苗,均通过了实验室的各项指标检测。经过在隔离器进行实验室安全和效力检验,以及田间试验证明,该疫苗安全有效,抗体水平高,维持时间长,能够保护鸡群抵抗新城疫、禽流感、传染性支气管炎等强毒的攻击。  相似文献   

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Epidemiologic methods are essential to understanding infectious diseases in aquaculture. Unfortunately, many of these methods are poorly understood or not utilized by fish-health scientists and aquaculturists — often because of the lack of contact with epidemiologists who are willing to investigate fish diseases. In this paper, we describe direct interactions between epidemiologists and fish-health specialists that have resulted in an improved understanding of the causes and management of infectious diseases in aquaculture. We focus on risk-factor studies, risk analysis and infectious-disease modeling, evaluation of diagnostic tests and experimental studies. We also describe characteristics of confined fish populations that make them ideal for developing and testing epidemiologic models and the theoretical and practical challenges of designing and conducting epidemiologic studies in fish farms. Throughout our presentation, emphasis is given to characteristics, opportunities and problems associated mainly with conducting epidemiologic studies to intensive aquaculture systems. We conclude that the development of increased cooperation among epidemiologists, fish-health scientists and aquaculturists will be mutually beneficial and, therefore, efforts for such collaboration should be initiated from all parties involved.  相似文献   

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Eighteen strains of Mycoplasma hyorhinis and a strain of Mycoplasma suipneumoniae were tested in 4 serological tests, i. e., disc growth inhibition, metabolic inhibition, indirect haemagglutination and indirect epi-immunofluorescence. Only with immunofluorescence could all tested strains of M. hyorhinis be shown; no cross-reactions between M. hyorhinis and M. suipneumoniae could be detected. The other tests failed in many cases to identify strains of the same species, and they gave cross-reactions between M. hyorhinis and M. suipneumoniae.  相似文献   

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Horses, donkeys, and mules have been important in Turkey for agriculture, transport, and the military for hundreds of years. Equids number more than 0.5 million in Turkey. Most horses are local types but emphasis is now on Thoroughbreds and Arabians for racing and competitions. New roles have not materialized for donkeys and mules that continue to perform their traditional activities. Disease control is assured mainly by public services acting within laws governing diseases and welfare. African horse sickness, glanders, dourine, equine infectious anemia, vesicular stomatitis, equine encephalomyelitis, anthrax, and rabies are notifiable diseases but none of the mainly equine diseases has been reported in Turkey for many years. Several zoonoses, including toxoplasmosis, brucellosis, listeriosis, and Rhodococcus infections, have been identified by serodiagnosis over wide areas, but animals carrying antibodies rarely exhibit clinical symptoms. Among other diseases in Turkish Equidae are piroplasmosis, respiratory infections, contagious equine metritis and equine influenza. Other viral and bacterial pathogens have been identified in isolated investigations. Internal and external parasites are a major cause of economic loss. Much research on equine diseases has been undertaken in the last decade of the 20th and first decade of the 21st centuries, perhaps because of Turkey's possible accession to the European Union and the goal to harmonize identification and control procedures and also because of the country's increasing participation in international equine events. This paper reviews animal health services and provides a bibliography of more than 100 references covering horse, donkey, and mule diseases in Turkey.  相似文献   

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