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1.
为解决三倍体西瓜种子的萌发问题,以三倍体西瓜种子为材料,研究了不同含水量的珍珠岩引发处理对三倍体西瓜种子萌发及其生理指标的影响.结果表明:引发处理不但显著提高了三倍体西瓜种子的发芽率和发芽速度,而且提高了苗期保护酶的活性及减少MDA的含量.不同处理产生不同的引发效果,其中珍珠岩含水量60%引发处理的效果最好.这说明适宜含水量的珍珠岩引发处理能够加快三倍体西瓜种子萌发速率和萌发一致性.  相似文献   

2.
不同引发方式对葫芦种子萌发的影响   总被引:1,自引:0,他引:1  
亢敏  别之龙  周虓  黄远 《北方园艺》2010,(24):18-21
以蛭石、赤霉酸(GA3)和聚乙二醇(PEG)为引发方式,研究了不同蛭石含水量、溶液处理浓度和处理时间对葫芦种子萌发的影响。结果表明:与对照相比,在适宜的引发条件下3种引发方式均可提高葫芦种子的发芽率,以蛭石含水量50%处理72 h效果最佳,种子发芽势、发芽率分别比对照提高64.10%和24.76%,发芽时间明显缩短。  相似文献   

3.
黑籽南瓜种子基质引发适宜条件研究   总被引:1,自引:1,他引:0  
以黑籽南瓜种子为材料,研究了不同温度、处理时间和基质含水量对黑籽南瓜种子引发效果的影响。结果表明,基质引发处理能明显提高黑籽南瓜种子发芽势、发芽指数和活力指数,明显减少种子平均发芽天数;基质引发温度明显提高幼苗干鲜质量和根体积等,而基质湿度对幼苗生长性状影响不显著。黑籽南瓜种子基质引发处理以5℃、基质湿度65%时激发种子活力的效果最好,在不同基质引发温度和湿度条件下,改变引发持续时间所产生的效果是不一样的。  相似文献   

4.
不同育苗基质对洋葱幼苗生长的影响   总被引:2,自引:0,他引:2  
张建 《长江蔬菜》2010,(12):64-65
采用5种基质进行洋葱育苗,结果表明,采用岩棉、蛭石草炭(V∶V=1∶1)、河沙、珍珠岩加菇渣(V∶V=1∶1)和土壤栽培的洋葱发芽率分别为67.7%,88.6%,81.7%,86.3%,81.2%;发芽势分别为63.4%,82.3%,72.0%,76.7%,56.7%;从壮苗指数看,蛭石草炭、河沙和珍珠岩加菇渣均高于土培和岩棉水培;根系活力以蛭石草炭为最高,其次为岩棉水培,土培最低;植株干物质积累量与基质养分含量呈正相关。  相似文献   

5.
以"奥运08"番茄为研究对象,以玉米秆、珍珠岩、蛭石为试材,研究了100%玉米秆、75%玉米秆+15%珍珠岩+10%蛭石、50%玉米秆+30%珍珠岩+20%蛭石、25%玉米秆+45%珍珠岩+30%蛭石4种复合基质处理对番茄生长的影响。结果表明:75%玉米秆+15%珍珠岩+10%蛭石的基质番茄植株各项生长指标均高于其它处理,产量也明显高于其它处理,因此该处理为玉米秆复合基质番茄的最佳基质配方。  相似文献   

6.
对管道水培生菜的6种育苗基质,即复合基质(泥炭:蛭石:珍珠岩=1:1:1)、陶粒、蛭石、珍珠岩、花泥、滤纸的育苗效果进行了比较研究.结果表明:6个处理的出苗率都较高,均在97%以上,但是花泥和滤纸出苗速度较慢.生菜苗期不同阶段的形态指标(幼苗株高、幼苗地上部鲜重、干重、地下部鲜重、干重等)以选用复合基质育苗效果均表现最佳,其次为陶粒、蛭石,花泥与珍珠岩效果接近,滤纸效果最差;而幼苗根系长度以选用蛭石进行育苗效果最佳,滤纸、复合基质、陶粒次之,花泥的育苗效果与珍珠岩接近.生菜苗期不同阶段的生理指标(叶绿素含量)以选用花泥表现效果最佳,复合基质次之,滤纸最差;而根系活力则以陶粒表现最佳,复合基质与滤纸次之,珍珠岩最差.不同基质育苗经过管道水培后,植株高度、地上部鲜重、干重等方面,选用蛭石均表现最佳,复合基质次之,花泥表现最差.综合来看,在该试验中,复合基质效果最好,其次为蛭石.但从育苗成本和可操作性综合来看,蛭石作为生菜育苗基质更为适合.  相似文献   

7.
以中枣1号为试材,用不同浓度的国繁2号为生根剂,研究了珍珠岩、蛭石、珍珠岩 蛭石 河沙、河沙对枣嫩枝扦插生根的影响.结果表明,用1 000 mg/L~2 000 mg/L生根剂处理插穗,然后扦插在珍珠岩 蛭石 河沙(1:1:1)混合基质中生根效果较好,平均生根数达24根以上,生根率为100 %,根长为6.2 cm以上.  相似文献   

8.
几种蔬菜种子贮存条件的探讨   总被引:1,自引:0,他引:1  
当前有关种子科学的研究结果认为,一般植物的种子大都适宜在较低的温度和含水量的状况下贮藏保存,但种子含水量低于5%也不利于种子的贮存。可是Woodstock, E.H.等人的研究却认为利用冷冻真空干燥的方法,把洋葱、辣椒等种子的含水量降低到2~5%时可提高种子的贮存性能。总之,对最适于种子贮存的含水量究竟应如何掌握,目前的看法仍不一致。Harrington,.F.认为在0℃~50℃的温度和5~14%种子含水量的范围内,均以温度和含水量愈低的贮存条件其贮存效果愈好。1985年国际遗传资源委员会(IBPGR)建议将资源库长期保存资源的库温由-10℃改为0°F…  相似文献   

9.
采用不同的处理措施,对药用植物高山红景天种子和插穗进行无土育苗试验.结果表明:种子繁殖中,温度处理和药剂处理均能明显地提高高山红景天种子的发芽率、出苗率和成活率,其中以变温处理效果最好,种子成活率可达58.67%.扦插无土育苗中IAA处理浓度为600 mg/L时对高山红景天插穗的成活率影响最大,扦插基质以草炭土∶蛭石∶珍珠岩=1∶1∶1为好.  相似文献   

10.
基质引发对辣椒种子发芽的影响   总被引:1,自引:0,他引:1  
以日本鹰爪辣椒和5个中国辣椒品种为试材,以珍珠岩为引发基质,研究了在珍珠岩中添加不同水量进行引发处理对辣椒种子发芽率的影响。结果表明:在珍珠岩中加入一定水分进行引发处理,可以提高种子发芽率,特别对缩短发芽天数、提高发芽的整齐度具有明显的促进作用。经过引发处理的种子,比未引发处理的可提前2~4 d完成发芽,提前4~6 d发芽率达到90%以上。采用珍珠岩作引发基质时,适宜的加水量为每1.5 g珍珠岩加0.4~0.6 mL蒸馏水,以21~24℃条件下引发处理4 d为宜。  相似文献   

11.
AIM: Although endovascular radiotherapy inhibits neointimal hyperplasia, the exact alterations induced by β-particles irradiation remain to be elucidated. The objective of this study was to investigate the ability and the cellular mechanism of local β-particles emission from 188Re to inhibit vascular smooth muscle cells (SMCs). METHODS: The SMCs in vitro were irradiated by 188Re with single doses of 2.6 Gy-25.8 Gy. The effects of β-particles on SMCs, such as effective irradiate doses, the period of inhibition for SMCs proliferation, the changes of cell proliferation rate and DNA synthesis rate, cell cycle progression and related gene expression, were investigated by cell count, [3H]-TdR incorporation, cell cycle progression analysis, cell viability and immunocytochemistry, respectivecy. RESULTS: β-particles irradiation with dose of 5.2 Gy could inhibit significantly SMCs proliferation. At dose of 20.6 Gy DNA synthesis inhibitory rate was 92%, SMCs proliferation rate was only 3%. Renoval of 188Re did not abolish the inhibitory effects of β-particles on SMCs proliferation. The expression of P53 was up regulation and PCNA was down regulation after irradiation. CONCLUSION: β-particles from 188 Re was significantly effective and permanent in inhibiting SMCs proliferation, and inhibitory effect was in dose-dependet manner ED50was 5 Gy, the best dose to inhibit SMCs proliferation was 20 Gy. β-particles irradiation induced SMCs to occur G0/G1 arrest, damaged the ability of SMCs reproliferation and led to cell clonogenic death. P53 and PCNA had regulatiory effects on SMCs proliferation after β-particles irradiation.  相似文献   

12.
AIM:To study the effect of L-Arg on plasma content of endothelin (ET) and the expression of proto-oncogene c-fos mRNA in the left ventricle of rats with renovascular hypertensive hypertrophy. METHODS: The level of c-fos mRNA were measured by in situ hybridization. The ET in plasma were measured by radioimmunoassay. RESULTS:After eight weeks of treatment with L-Arg, the expression of c-fos decreased markedly (P<0.01). The ET content in plasma also decreased significantly by L-Arg(P<0.01).CONCLUSION: Plasma ET content and the expression of c-fos in the left ventricle of rats with renovascular hypertensive hypertrophy could be decreased by L-Arg administration.  相似文献   

13.
Abstract

Saskatoon berry (Amelanchier alnifolia Nutt., Rosaceae) and blueberry (Vaccinium corymbosum L., Ericaceae) are substantially equivalent in all characteristics that are important to the consumer, including fruit color, shape, size, nutrition, texture, and uses. In addition, both fruits are native to North America and they have practically identical historical uses and known health benefits. Their composition, processing, nutritional value and metabolism, intended uses, and levels of undesirable substances are compared.  相似文献   

14.
多效唑对猕猴桃离体试管苗生长及内源激素的影响   总被引:18,自引:0,他引:18  
多效唑(PP333)处理猕猴桃试管苗,降低了其生长强度;植株体内的GA3、IAA和ZT含量下降,ABA的含量上升,乙烯释放率增加;并且能降低外源的GA3和IAA促进生长的作用,而外源的GA3和IAA又能不同程度地逆转多效唑的抑制作用,使植株恢复生长。  相似文献   

15.
AIM: To investigate and screen the sensitive proteins in the formation mechanism of pathological scars by comparing the results of differential proteomic analysis between pathological scars and normal skin.METHODS: Two-dimensional gel electrophoresis was used to detect the protein expression profiles in 8 keloid patients, 8 hypertrophic scar patients and 3 matched normal skin patients.The proteins that showed differential expression of over 4-fold change were cut and analyzed by MALDI-TOF/TOF mass spectrometry.RESULTS: A two-dimensional protein profiling comparison between pathological scars and normal skin was successfully established.On average, 2 978 spots in keloid, 2 975 spots in hypertrophic scar and 3 053 spots in normal skin were identified using gel analysis software.Compared with normal skin, there were totally 36 differentially-expressed proteins in keloid and hypertrophic scar identified from the spots of over 4-fold change, including 16 proteins in both keloid and hypertrophic scar (8 up-regulated and 8 down-regulated), 11 only in keloid (9 up-regulated and 2 down-regulated) and 9 only in hypertrophic scar (4 up-regulated and 5 down-regulated).CONCLUSION: Proteomic analysis can identify the proteins with variance of pathological scars versus normal skin, thus providing probable new clues to reveal the formation mechanism of pathological scars.  相似文献   

16.
The objective of this study was to establish a cryopreservation protocol for hawthorn shoot apices (Crataegus pinnatifida Bge.). Cryopreservation was carried out via encapsulation–dehydration, vitrification, and encapsulation–vitrification on shoot apices excised from in vitro cultures. We began by showing that cold-acclimation enhanced the regrowth of cryopreserved apices from 10.0 to 65.5% in encapsulation–dehydration. We then decided that the encapsulation–dehydration method was an optimal cryopreservation method for hawthorn shoot apices in terms of its high recovery after cryopreservation as well as its ease of use compared with vitrification and encapsulation–vitrification. In encapsulation–dehydration, the protocol leading to optimal regrowth was as follows: after cold-acclimation at 5 °C in the dark for 2 weeks, excised shoot tips were pretreated for 24 h at 25 °C on hormone-free Murashige and Skoog [Murashige, T., Skoog, F., 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15, 473–497] (MS) basal medium with 0.4 mol/L sucrose, then encapsulated and precultured in liquid MS medium with 0.8 mol/L sucrose for 16 h at 25 °C. Precultured beads were dehydrated for 6 h at 25 °C in the dessicator containing 50 g silica gel to a moisture content of 15.3% (fresh-weight basis) before cryostorage for 1 h. In addition, we examined the effect of adding glycerol to both the alginate beads and loading solution to enhance regrowth after cryopreservation in encapsulation–dehydration. In the present study, it was shown that adding 0.5 mol/L glycerol resulted in high regrowth percentages (82.5–90.0%) in four Crataegus species.  相似文献   

17.
AIM:To investigate the effect of metallothionein(MT) on proliferation of rat vascular smooth muscle cells (VSMCs) stimulated by homocysteine and its mechanism. METHODS:VSMCs proliferation was measured by [3-H]-TdR incorporation, mitogen-activated protein kinase(MAPK)activity were determined by immunoprecipitation method, the intracellular contents of MT and malondialdehyde (MDA)were assayed by -hemoglobin saturation method and TBA reaction, respectively, and lactate dehydrogenase (LDH) leakage was measured by NADH oxidation. RESULTS:Hcy(10-6-10-4 mmol/L) stimulated [3-H]-TdR incorporation by the VSMCs in a concentration-dependent manner. Compared with control, [3-H]-TdR incorporation in VSMCs treated with 0.1 mmol/L Hcy was increased by 4.2 fold (P<0.01). Meanwhile, Hcy enhanced MAPK activity, MDA formation and LDH release (P<0.01)in a concentration-dependent manner. Treatment of VSMCs with MT alone did not change above parameters, compared with control. However, MT (10-6-10-4 mol/L)attenuated significantly Hcy-stimulated proliferation of VSMCs (P<0.01)in a concentration-dependent manner. And MT inhibited obviously Hcy-induced activation of MAPK activity, MDA formation and LDH release. Preincubation of VSMCs with 0.5 mmol/L ZnCl2 for 6 h induced an increase cellular MT content by 5.7-fold (P<0.01). The MT-overexpressed VSMCs resisted Hcy-stimulating action on MAPK activity, MDA formation and LDH leakage (P<0.01). CONCLUSION:These results show that MT has an inhibitory effect on Hcy-induced VSMCs proliferation, and that MT could inhibit Hcy-stimulated MAPK activity and lipid peroxidation.  相似文献   

18.
Historic landcover dynamics in a scrubby flatwoods (Tel-4) and scrub landscape (Happy Creek) on John F. Kennedy Space Center were measured using aerial images from 1943, 1951, 1958, 1969, 1979, and 1989. Landcover categories were mapped, digitized, geometrically registered, and overlaid in ARC/INFO. Both study sites have been influenced by various land use histories, including periods of range management, fire suppression, and fire management. Several analyses were performed to help understand the effects of past land management on the amount and spatial distribution of landcover within the study sites. A chi-squared analysis showed a significant difference between the frequency of landcover occurrence and management period. Markov chain models were used to project observed changes over a 100-year period; these showed current management practices being effective at Tel-4 (restoring historic landscape structure) and much less effective at Happy Creek. Documenting impacts of past management regimes on landcover has provided important insight into current landscape composition and will provide the basis for improving land management on Kennedy Space Center and elsewhere.  相似文献   

19.
AIM: Previous studies performed with XBP-01 in vitro indicated that XBP-01 could inhibit vascular smooth muscle cells from being transformed into foam cell and could eliminate the atherosclerotic plaque in C57BL/6J mouse. This experiment is to investigate its mechanism of eliminating plaques in vitro. METHODS: The cultured porcine artery smooth muscle cells incubated with XBP-01 of 0.1 mg/L for 24 h after preincubated with oxidized low density lipoprotein of 15 mg/L for 72 h in vitro. The samples were analyzed by fluorescence microscope, confocal microscope system and flow cytometry. RESULTS: Apoptosis was triggered by being incubated with oxidized low density lipoprotein and this process was accelerated additionally by being incubated with XBP-01. CONCLUSION: XBP-01 can be effective in eliminating atherosclerotic plaque by accelerating the process in which oxidized low density lipoprotein induced smooth muscle cell apoptosis.  相似文献   

20.
Species distribution models (SDMs) often use elevation as a surrogate for temperature or utilise elevation sensitive interpolations from weather stations. These methods may be unsuitable at the landscape scale, especially where there are sparse weather stations, dramatic variations in exposure or low elevational ranges. The goal of this study was to determine whether radiation, moisture or a novel estimate of exposure could improve temperature estimates and SDMs for vegetation on the Illawarra Escarpment, near Sydney, Australia. Forty temperature sensors were placed on the soil surface of an approximately 12,000 ha study site between November 2004 and August 2006. Linear regression was used to determine the relationship with environmental factors. Elevation was correlated more with moderate temperatures (winter maximums, summer minimums, spring and autumn averages) than extreme temperatures (summer maximums, winter minimums). The correlation (r 2) between temperature and environmental factors was improved by up to 0.38 by incorporating exposure, moisture and radiation in the regressions. Summer maximums and winter minimums were predominately determined by exposure to the NW and coastal influences respectively, while exposure to the NE and SW was important during other seasons. These directions correspond with the winds that are most influential in the study area. The improved temperature estimates were used in Generalised Additive Models for 37 plant species. The deviance explained by most models was increased relative to elevation, especially for moist rainforest species. It was concluded that improving the accuracy of seasonal temperature estimates could improve our ability to explain the patchy distribution of many species. Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.  相似文献   

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