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1.
为了解新疆北疆地区猪支原体肺炎的分子流行病学特征,对新疆北疆5个地区的部分规模化猪场进行了调查,采用PCR方法对疑似病料进行了ldh基因检测,将阳性产物测序与在GenBank已登录的标准菌株序列进行同源性分析。结果显示:214份病变肺脏中,阳性病料为122份,阳性率为57.01%(122/214),ldh基因序列与参考菌株同源性为97.3% 100%。检测结果表明新疆北疆部分地区感染猪肺炎支原体情况比较严重。 相似文献
2.
为了解天津部分猪场副猪嗜血杆菌病(HPs)和猪传染性胸膜肺炎(PCP)的流行情况,利用间接血凝试验对采自9个规模猪场的178份猪血清样品进行副猪嗜血杆菌抗体检测,对采自8个规模猪场的166份猪血清样品进行猪传染性胸膜肺炎放线杆菌抗体检测。结果表明:副猪嗜血杆菌阳性率为18.54%(33/178),其中断奶仔猪和保育阶段猪阳性率为23.58%(25/106);猪传染性胸膜肺炎的阳性率为21.08%(35/166),其中断奶仔猪阳性率为20.93%(9/43),育肥猪阳性率为27.66%(13/47),种猪阳性率为21.62%(8/37)。说明天津地区部分猪场的副猪嗜血杆菌病和猪传染性胸膜肺炎呈地方性流行。 相似文献
3.
猪支原体肺炎又称猪气喘病,是危害养猪业最重要的疾病之一。本研究通过采集新疆伊宁市周边地区规模化猪场、养殖合作社及生猪散养户饲养的疑似支原体肺炎猪只的鼻拭子或病料(肺脏),利用荧光定量PCR方法对猪支原体肺炎感染情况进行调查,并结合对周边规模化养殖场、养殖合作社和散养户进行的支原体肺炎流行病学调查进行分析,结果显示伊宁市周边规模化养殖场、养殖合作社及周边散养户的猪只均有猪支原体肺炎感染,其中鼻拭子采样阳性率为38.8%;肺脏病料阳性率为57.8%,结果也反映了散养户发生支原体肺炎感染的情况较为严重。同时通过支原体肺炎的流行病学调查结果综合分析该病的发生及发展规律对预防控制新疆地区猪支原体肺炎提供理论依据。 相似文献
4.
用PCR方法对来源于湖南省株洲市、益阳市、长沙市、岳阳市、邵阳市、衡阳市等6个地区部分规模猪场的322份屠宰猪肺样品进行猪肺炎支原体检测。结果发现,猪肺炎支原体在该地普遍存在,检出率分别为:株洲市19.2%(11/57),益阳市18.1%(8/44),长沙市20.0%(13/65),岳阳市17.5%(7/40),邵阳市23.2%(13/56),衡阳市25.0%(15/60)。序列分析表明,试验的PCR产物与国内已出版的相关序列分别有98%~100%的同源性。试验结果证实了猪肺炎支原体在湖南省规模化猪场不同程度存在,对湖南省防控猪支原体肺炎提供了数据参考。 相似文献
5.
为了解石河子垦区规模猪场猪流感病毒H1N1亚型(SIV)感染情况,采集石河子垦区6个规模猪场血清,采用血清学方法进行HIN1亚型猪流感病毒抗体检测,同时调查混合感染情况。结果:186份血清样品H1N1亚型SIV抗体阳性率为27.42%(51/186),6个猪场感染阳性率为50%(3/6);51份阳性血清中,82.35%(42/51)感染了猪肺炎支原体(Mhp),80.39%(41/51)感染了猪圆环病毒2型(PCV2),29.41%(15/51)感染了猪胸膜肺炎放线杆菌(APP),50.98%(26/51)感染了副猪嗜血杆菌(HPS),62.74%(32/51)感染了猪链球菌2型(SS2)。说明垦区部分规模猪场存在H1N1亚型SIV感染情况,且混合感染情况比较严重。 相似文献
6.
为了解副猪嗜血杆菌(HPS)在石河子规模猪场的流行情况,试验采用ELISA方法进行了副猪嗜血杆菌抗体的检测,并对抗体阳性血清进行了其他呼吸道病混合感染血清学调查。结果表明:330份血清样品HPS抗体阳性率为60.30%(199/330),12个猪场感染阳性率为91.67%(11/12);199份阳性血清中,24.62%(49/199)感染了H1N1亚型猪流感病毒(SIV),49.25%(98/199)感染了猪肺炎支原体(Mhp),84.42%(168/199)感染了猪圆环病毒2型(PCV-2),35.18%(70/199)感染了猪胸膜肺炎放线杆菌(APP),49.75%(99/199)感染了猪链球菌2型(SS2)。说明垦区大部分规模猪场存在HPS感染情况,且混合感染情况比较严重。 相似文献
7.
猪气喘病在我国广泛流行,为查清该病在贵州省流行情况,笔者对部分猪场和散养户进行了流行病学调查,证实猪群咳嗽、气喘现象较为普遍;对疑似病猪、死猪采集病料,用PCR试剂盒检测猪肺炎支原体病原阳性率为25%;对猪群抽检血样,用ELISA试剂盒检测猪肺炎支原体血清抗体阳性率为30%以上。以上调研证明,贵州猪场猪支原体肺炎感染较为严重,这与临床调查结果基本相符。分析原因可能与贵州气候、养猪户不重视保温、绝大多数养猪户不接种猪肺炎支原体疫苗有一定关系,这为今后我省防治该病提供了重要依据。 相似文献
8.
为了解新疆部分地区绵羊包虫病流行情况,笔者采用ELISA法检测新疆35个县（市、区）共计1450份绵羊血清样本。结果显示：新疆部分地区绵羊包虫病血清阳性率为11.66%(169/1450),以铁门关市绵羊包虫病血清阳性率较高,为23.08%;不同调查点绵羊包虫病血清阳性率统计学差异显著（P<0.05）。规模化场和散养绵羊包虫病血清阳性率分别为10.89%(33/303)和11.86%(33/303),无统计学差异（P>0.05）。新疆南疆地区、北疆地区和东疆地区绵羊包虫病血清阳性率分别为13.16%(139/1056)、6.54%(17/260)和9.70%(13/134),不同区系绵羊包虫病血清阳性率不具有统计学差异（P>0.05）。调查结果表明,新疆地区绵羊包虫病感染仍较为普遍,应持续加强犬的管理和驱虫,并给农牧民普及包虫病防治知识。 相似文献
9.
为了解新疆巴州部分地区猪感染弓形虫病的现状,本试验以弓形虫Tg SAG2蛋白作为包被抗原对巴州地区433份猪血清样品进行了ELISA检测。结果显示,平均阳性率为12.70%(55/433)。其中,散养猪场阳性率为16.83%(35/208),规模化养殖场为9.94%(16/161),散养猪场阳性检出率显著高于规模化养殖场(P0.05);在各县市采集的血清样品中,尉犁县阳性率最高,为26.53%(13/49),和静县和焉耆县阳性率最低,分别为6.52%(3/46)和6.82%(3/44)。本次试验大范围地调查了巴州地区猪弓形虫病的流行现状,为该地区弓形虫病的防控奠定了理论依据。 相似文献
10.
猪肺炎支原体是呼吸系统疾病综合征(PRDC)的重要病原,由此引起的喘气病给猪场造成显著的经济损失。了解目前规模化猪场猪肺炎支原体的感染动态对于制定相应的科学防疫策略大有帮助。本研究采用阻断ELISA方法对我国部分地区规模化猪场猪肺炎支原体血清流行病学状况进行了系统监测与分析,6个未免疫喘气病疫苗的规模化猪场全部为血清学阳性场,其中种猪的阳性率为90.5%,仔猪的阳性率为31.1%。后备、1～2胎、3～4胎、5～6胎以及大于6胎种猪抗体阳性率分别为100%、100%、95.2%、85.7%以及71.4%。4、7、10、13、16、20、24周龄仔猪抗体阳性率分别为16.2%、0%、0%、16.7%、35.3%、71.4%、100%。这表明我国规模化猪场肺炎支原体感染非常普遍,种猪和后期育成猪是猪场的主要传染源,但部分猪场存在早期感染,由于喘气病发展进程缓慢,因此规模化猪场应根据猪场生产体系、管理水平、感染压力和感染动态制定合适的免疫方案,并采取综合措施控制由支原体肺炎造成的损失,提高猪场生产指标。 相似文献
11.
In late December 1985, 1485 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1985 and 44% of them responded. Veterinarians reported that 137,300 dogs were blood-tested to check for microfilariae and 1210 dogs were found with heartworm. Another 36 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number diagnosed in 1985 as 1247 (0.91%). Heartworm was reported from all provinces except Prince Edward Island, Newfoundland and Saskatchewan but most (1126) of the cases were in Ontario. Southwestern Ontario continued to be the primary focus of the infection in Canada. From Quebec, 91 cases were reported mostly from and around Montreal. From Manitoba, 19 cases were reported from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 28% of the cases were observed with clinical signs of heartworm disease and 78% had a history of not having left Canada. 相似文献
12.
In late November 1988, 1581 small and mixed animal clinics and institutional veterinarians across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1988, and 46% of them responded. Veterinarians reported that 181,577 dogs were blood-tested for heartworm disease and 367 dogs were found with D. immitis microfilariae. Another 60 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1988 as 441 (0.24%). 相似文献
13.
In late December 1984, 1853 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1984 and 35% of them responded. Veterinarians reported that 97,794 dogs were blood-tested to check for microfilariae and 1417 dogs (1.45% of those tested) were found with heartworm. Another 34 dogs were amicrofilaremic, but were diagnosed as having heartworm disease, to give the total number diagnosed in 1984 as 1451 (1.48%). Heartworm was reported from all provinces except Prince Edward Island and Newfoundland but most (1310) of the cases were in Ontario. In Quebec, 126 cases were reported mostly from west of Montreal. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 27% of the cases were observed with clinical signs of heartworm disease and 72% had a history of not having left Canada. Southwestern Ontario continued to be the primary focus of the infection. 相似文献
15.
In late November 1991, 1883 clinics in Canada were sent a questionnaire to assess the status of Dirofilaria immitis in dogs in 1991 and there was a 60.0% response. There were 344,031 dogs tested for heart-worm (HW), 627 were found infected and the prevalence of HW infection was 0.18%. There were 417 dogs with HW in Ontario, 116 in Manitoba, 38 in Quebec, 53 in British Columbia, three in Alberta, and one in Nova Scotia. In British Columbia, all of the infected dogs but one were from the Okanagan valley which, as from 1991, is a new focus of infection in Canada. Most dogs with HW had not been on preventive medication in 1990, and the prevalence among dogs tested and unprotected was 0.59%. That prevalence was considerably higher in endemic areas. Companion dogs, over three years of age and maintained primarily outdoors in rural areas, were most frequently infected. One cat was diagnosed with D. immitis and 33 dogs had Dipetalonema reconditium. 相似文献
16.
In late November 1989, 1732 clinics and institutional veterinarians were sent a questionnaire to assess the status of Dirofilaria immitis, and 51.7% responded. Of 247,716 dogs tested, 394 had D. immitis microfilariae and 51 were amicrofilaremic for a total of 445 cases and heartworm prevalence of 0.17%. Most (408) of these dogs had no preventive medication and the prevalence among dogs tested and unprotected was 1.01%. That prevalence was considerably higher in endemic areas. Thirty-seven dogs with heartworm had preventive medication. Heartworm was most frequent in companion dogs over three years of age maintained outdoors in rural areas. About 75% of the cases had never left Canada, 26% had clinical signs and 125 were not treated. Heartworm was reported from British Columbia, Manitoba, Ontario, Quebec, Nova Scotia and Newfoundland, but 383 cases were in Ontario. South-western Ontario was the primary focus of infection. There were 33 cases in Quebec and 24 in Manitoba, mainly found in and around Metropolitan Montreal and Winnipeg respectively. 相似文献
17.
In late November 1987, 1246 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1987, and 50% of them responded. Veterinarians reported that 165,428 dogs were blood tested for heartworm disease and 511 dogs were found with D. immitis microfilariae. Another 78 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1987 as 589 (0.35%). 相似文献
18.
In late December 1986, 1224 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1986; 46% of them responded. Veterinarians reported that 150,989 dogs were blood-tested for microfilariae and 869 dogs were found with heartworm. Another 65 dogs were amicrofilaremic but diagnosed with heartworm disease and one was found with heartworm at necropsy to give the total number diagnosed in 1986 as 935 (0.62%). Heartworm was reported from Manitoba, New Brunswick, Ontario and Quebec, but most (810) of the cases were from Ontario. South-western Ontario continued to be the primary focus of the infection in Canada. There were 103 cases reported from Quebec, mostly from and around Montreal, and 21 cases from Manitoba, from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 33% of the cases were observed with clinical signs of heartworm disease and 81% had a history of not having left Canada. 相似文献
20.
In late December 1983, 2 800 veterinarians across Canada were sent a questionnaire in order to assess the status of heartworm disease in Canada in 1983 and 26% of them responded. Veterinarians reported that 59 504 dogs were blood-tested to check for microfilariae and 771 dogs (1.30% of those tested) were found with Dirofilaria immitis. Heartworm disease was diagnosed in all provinces except New Brunswick and Newfoundland but most (733) of the cases were in Ontario. Heartworm disease was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 31% of the cases were observed with clinical signs of heartworm disease and 64% had a history of not having left Canada. Southwestern Ontario continues to be the focus of the infection and most of the dogs there had not left the province previously. 相似文献
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