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1.
Popcorn inbred lines with more efficient nitrogen use are better able to uptake nitrogen from the soil and convert it into higher grain yield, resulting in lower environmental and economic impacts caused by nitrogen fertilization. The objectives of this study were to (i) identify inbred lines superior in nitrogen use (ii) assess the genetic diversity between popcorn inbred lines under high and low N conditions and (iii) investigate the causal effects of several traits in nitrogen use efficiency (NUE). We evaluated 25 popcorn inbred lines under high and low N, and several traits related to NUE and its components were measured. Efficient and inefficient inbred lines under both N levels were identified and can be useful for generating a segregating population for quantitative trait loci mapping. The genetic diversity assessment based on phenotypic traits grouped the inbred lines into four clusters under both N levels, and the efficient inbred lines were grouped together, as were the inefficient lines. The most divergent inbred lines under high N were classified as efficient and intermediate and can be useful to generate a divergent breeding population with a high frequency of favorable genes for NUE. Nitrogen uptake efficiency (NUpE) was considered the most important trait for NUE. To improve the accuracy of selection for NUE, a selection index involving the total root length (TRL), daily growth and NUpE traits under both N levels is recommended, as these traits had high correlations with and direct effects on NUE.  相似文献   

2.
Wheat genotypes that efficiently capture and convert available soil nitrogen into harvested grain protein are key to sustainably meeting the rising global demand for grain protein. The purposes of this study were: to characterize the genetic variation for nitrogen use efficiency (NUE) traits within hard winter wheat adapted to the Great Plains of the United States and evaluate trends in the germplasm with year of release; to explore relationships among traits that may be used for selection within breeding programs; and to identify quantitative trait loci associated with NUE traits in this germplasm. NUE traits were measured in a panel of 299 hard winter wheat genotypes, representing historically important and contemporary germplasm, from across the growing region. Trials were grown in two years at two levels of nitrogen fertility. Genotype and genotype × year interaction effects were highly significant for NUE traits, while genotype × nitrogen rate interactions were non-significant. Strong genetic correlations of plant height and flowering date with NUE traits were observed. Wheat breeders have improved NUE: the subset of 183 genotypes that were released as cultivars after 1960 demonstrated significant trends with year of release for improved grain N yield, grain yield, nitrogen harvest index, nitrogen uptake efficiency, nitrogen utilization efficiency, and post-anthesis nitrogen uptake. In genome-wide association analyses, plant height and flowering date were important covariates in the mixed models, and plant height and flowering date substantially explained the variation in NUE traits in this germplasm. Marker-trait associations were identified that may prove useful in breeding.  相似文献   

3.
The benefits of improving nitrogen use efficiency (NUE) in crops are typically studied through the performance of the individual crop. However, in order to increase yields in a sustainable way, improving NUE of the cropping systems must be the aim. We did a model simulation study to investigate how improvement of NUE traits of individual crops affects the succeeding crops and the NUE of the crop rotation. Based on experimental results parameterization was altered for different types of improved NUE in the EU-Rotate_N model, e.g. through higher N harvest index, reduced litter loss or improved root depth penetration rate. The different ways of improving NUE have different effects on the cropping system, affecting either N uptake, the ability of the crop to hold on to N already taken up, or the fraction of crop N being harvested. Due to the different modes of action, the model simulations show that these changes in NUE traits will also have different effects on N leaching loss and on N availability and N loss in the following years. Simulations also show that the effect of genotypes with improved NUE depend on environment and crop management. This is true for the improved crop itself and when its effect is analyzed for the whole cropping system. The environmental conditions, crop choices and management will all affect the fate of the N left in the soil, and whether this will contribute mainly to leaching loss or be used for production in later crops. As an example, increasing pre-crop fertilization was shown to affect the leaching after the following oilseed rape crop with up to 50 kg N ha?1 taken up before it was lost to the environment when pre-crop fertilization as well as root depth penetration rate was high. All in all, the simulations illustrate the concept of NUE as the result of interactions between genotype, environment and crop management (G×E×M).  相似文献   

4.
马铃薯是我国主要粮食作物之一,马铃薯分子育种研究具有重要意义。在二倍体马铃薯中,重要性状控制基因的QTL定位及克隆已经有大量报道。近年,随着同源四倍体分析软件的开发,四倍体马铃薯的遗传图谱构建和QTL定位也取得了突破性进展。分子标记是马铃薯育种的重要辅助手段,可快速准确筛选出多个优良性状。对马铃薯重要农艺性状的QTL定位和克隆,以及3个抗病性状分子标记辅助选育的研究进展进行概述,为加快马铃薯分子育种研究提供参考和实践依据。  相似文献   

5.
Potato has a variety of reproductive uniquenesses besides its clonal propagation by tubers. These traits are controlled by a different kind of genetic control. The reproductive information has been applied to enable interspecific hybridization to enhance valuable traits, such as disease and pest resistances, from the tuber-bearing Solanum gene pool. While progress has been made in potato breeding, many resources have been invested due to the requirements of large populations and long time frame. This is not only due to the general pitfalls in plant breeding, but also due to the complexity of polyploid genetics. Tetraploid genetics is the most prominent aspect associated with potato breeding. Genetic maps and markers have contributed to potato breeding, and genome information further elucidates questions in potato evolution and supports comprehensive potato breeding. Challenges yet remain on recognizing intellectual property rights to breeding and germplasm, and also on regulatory aspects to incorporate modern biotechnology for increasing genetic variation in potato breeding.  相似文献   

6.
陕西省不同年代玉米品种产量和氮效率性状的变化   总被引:2,自引:0,他引:2  
明确陕西省不同年代玉米产量和氮效率性状响应氮肥的变化趋势,对西北旱区玉米高产氮高效品种选育具有重要的实践意义。本文以1981—2010年间陕西省12个玉米主栽品种为材料,于2011年和2012年在典型旱区陕西长武进行3个氮水平(0、120和240 kg hm–2)的田间试验,分析了不同年代玉米品种农艺和氮效率性状变化趋势。结果显示,不同年代玉米品种籽粒产量随氮水平增加而提高,在施氮0、120和240 kg hm–2处理下籽粒产量增益分别为每年46、65和83 kg hm–2。所有氮水平下2000—2010年间品种产量和生物量显著高于1980—1989年间品种,而秸秆产量变化不明显;现代玉米品种(2000—2010年)产量的增加归因于穗粒数、千粒重和生物量的提高。不同年代玉米品种消光系数随氮水平增加而降低,说明现代玉米品种(2000—2010年)较老品种(1980—1989年)叶片直立,截获更多的光能,致使产量和生物量高。随着年代的递进,玉米品种氮肥农学利用率呈递增趋势,在低氮水平下现代品种氮肥利用效率最高,且显著高于老品种。氮肥农学利用率与氮吸收效率(NUpE)和花后氮素积累量呈显著相关(r=0.75;r=0.72),而与氮生理效率(NUtE)和花前氮素积累量相关性不显著(r=0.42;r=0.39)。说明现代玉米品种氮肥农学利用率提高主要来自氮肥吸收效率和花后氮素积累量的增加。上述结果表明,陕西玉米育种应注重穗粒数、千粒重、氮吸收效率性状和株型结构改良,低氮环境压力选择将有助于旱区玉米高产氮高效新品种培育。  相似文献   

7.
One approach to decrease the environmental impact of crop production and reduce costs is to optimize agronomic practices and genotypes so that nutrients are used more efficiently. In this study the effects of agronomic practices (rotations, crop protection, fertilization) on yields, nitrogen use efficiency (NUE) and associated parameters were studied in an experiment using two winter wheat genotypes (Cordiale and Scaro) in one season and two potato genotypes (Sarpo Mira and Sante) in two seasons. The wheat showed no varietal differences in yield and NUE; instead the fertilization regime was the main factor affecting yield and NUE with higher values observed when conventional fertilization was used. The exception was for wheat grown after three years grass/clover ley when there was no added yield benefit from conventional fertilization of the organically bred variety (Scaro). This demonstrates the potential for N fixing crops to provide sufficient N to high yielding cereals if grown for long enough prior to planting. The greatest gains in NUE were achieved by combining an N efficient genotype with conventional crop management in an organic rotation. Fertilization and genotypic variation were the main factors affecting potato tuber yield and NUE, with the late maturing Sarpo Mira displaying elevated yields and NUE compared with the early maturing Sante. The use of organic fertility sources resulted in lower NUE, but N release from organic sources may increase NUE of future crops. This highlights the need for long-term nutrient balance and modelling studies to assess NUE at the crop rotation scale.  相似文献   

8.
绿色超级稻品种的农艺与生理性状分析   总被引:2,自引:0,他引:2  
探明绿色超级稻的农艺与生理性状,对于培育和选用绿色超级稻品种有重要意义。本研究以4个绿色超级稻品种为材料,1个超级稻品种和1个非超级稻品种为对照,观察了绿色超级稻的农艺与生理表现。结果表明,与对照品种相比,绿色超级稻品种具有较高的产量和氮素利用效率。绿色超级稻品种较高的产量得益于总颖花数和结实率的同步提高,较高的氮素利用率主要在于较高的植株氮素籽粒生产效率(氮素内部利用效率)。绿色超级稻具有较高的茎蘖成穗率和粒叶比,抽穗期较高的糖花比,灌浆期较高的作物生长速率、净同化率、根系氧化力和茎中同化物向籽粒的运转率和成熟期较高的收获指数。这些性状与产量及植株氮素籽粒生产效率均呈极显著的正相关。建议将上述性状作为培育和选用绿色超级稻品种的参考指标。  相似文献   

9.
不同氮敏感性粳稻品种的氮代谢与光合特性比较   总被引:3,自引:0,他引:3  
本文旨在探明不同氮敏感性粳稻品种氮代谢与光合特性。以2个氮敏感高产品种淮稻5号和连粳7号, 2个氮钝感品种宁粳1号和扬粳4038为材料, 设置0和200 kg hm-2 2个施氮水平, 研究其产量、氮肥利用效率以及地上部生理性状的变化特点。结果表明, 在2种施氮水平下, 氮敏感品种的产量和氮肥利用效率显著高于氮钝感品种。与氮钝感品种相比, 氮敏感品种具有较高的光合速率和氮素积累, 较强的氮代谢酶活性和较高的光合氮素利用效率, 抽穗期茎叶中积累较多的可溶性糖和淀粉, 抽穗至成熟期茎鞘中非结构性碳水化合物向籽粒转运率较高。表明氮敏感品种在较低施氮量下具有较高的生理活性和物质生产效率; 这些特征可作为筛选高产氮敏感水稻品种的重要生理指标。  相似文献   

10.
The physiological responses of potato (Solanum tuberosum L) cultivars to soil drought at the tuberization phase and their impact on agronomically important traits were investigated in potted plants under semi‐controlled conditions. Genotype‐dependent responses of potato to water deficiency were evaluated on two pairs (tolerant/sensitive) of Polish cultivars. Each pair of cultivars had a similar genetic background but was extremely different in terms of drought tolerance evaluated on the basis of loss of tuber yield under field conditions. The results clearly indicate different mechanisms of tolerance to water deficiency and the ability to recover from soil drought in two tolerant but genetically unrelated cultivars. When subjected to soil drought, the cultivar Gwiazda had low rates of transpiration and photosynthesis and low levels of stomatal conductance due to hypersensitivity to ABA, but its maximal photochemistry efficiency and PSII performance index were unchanged. Another strategy was displayed by the dehydration‐avoidant cultivar Tajfun, which kept the stomata partially open. Thus, the plants were able to retain a relatively high rate of photosynthesis over transpiration. The parameters measured for cultivar Tajfun for photosynthesis and transpiration were the same after plant rewatering, similar to the control plants. This was not the case for the cultivar Gwiazda. The ability of plants to regenerate after soil drought relief appears to be a good indicator of potato susceptibility to soil drought and allows the yield of potato tubers to be predicted. The physiological traits identified in closely related potato cultivars but differed in their drought tolerance seem to be useful for genetic engineering and breeding programmes.  相似文献   

11.
Spinach is a leafy vegetable that requires a high N fertilization to have a satisfactory yield and quality, in part because it has poor nitrogen use efficiency (NUE). Therefore, there is a need to breed for cultivars with an excellent NUE. To this end the genetic diversity for NUE-related traits was studied in a diverse set of commercial cultivars. This set was evaluated in a hydroponic system using the Ingestad model; the system was set at a relative growth rate of 0.14 and 0.18 g g?1 day?1 (low and high N, respectively). Experiments were performed at low and high plant density. Traits monitored for single plants included fresh and dry weight, leaf area, specific leaf area, dry weight ratio between root and shoot, and chlorophyll content. The high density experiment showed more genotypic variation for the observed traits than the low density one. Biomass production was considerably lower at low than at high N. Path analysis revealed that leaf area had the highest direct effect on NUE, while specific leaf area was an important trait determining variation in NUE at low N. Slow and fast growing genotypes were shown to use different strategies to utilize N, and these strategies are expressed differently at high and low N availability. This indicates that improving spinach for NUE is feasible using the analysed genotypes as source material, and different strategies can be targeted for adaptation of spinach cultivars to low N conditions.  相似文献   

12.
阐明水稻品种演进过程中植株生产力和资源利用效率的变化特征,对高产高效协调的水稻品种选育和稻作技术改进具有重要的理论参考意义。本研究以1950-2010年间辽宁省的12个水稻主栽品种为材料,探讨氮肥施用对不同年代水稻品种植株生产力和氮肥利用效率的影响。结果表明,1990s及之后品种的最高生物学产量和籽粒产量均显著高于1980s及以前的品种,1990s及之后品种产量的递增主要归功于穗粒数的增加。其最高产量的施氮量显著高于1980s及以前的品种,低氮条件下不同年代品种间的生产力差异不显著。随年代的递进,水稻品种的氮肥农学利用率、偏生产力呈现递增趋势,但生理利用率和吸收利用率的变化趋势不明显;茎叶氮含量变化不明显,但籽粒氮含量呈下降趋势。随施氮量的递增,各年代品种植株含氮量均呈现递增趋势,但农学利用率、偏生产力、生理利用率、吸收利用率均呈下降趋势。在低氮条件下1990s及之后品种的氮肥利用效率与1980s及以前品种的差异不明显,但高氮下前者的氮肥利用效率显著高于后者。上述结果表明,虽然辽宁水稻植株生产力提升是品种改良和施肥水平提高的双重作用的结果,但在今后品种选育和栽培技术改进中要进一步协调好高产与资源高效的关系。  相似文献   

13.
This review presents an overview of accomplishments on different aspects of cowpea breeding for drought tolerance. Furthermore it provides options to enhance the genetic potential of the crop by minimizing yield loss due to drought stress. Recent efforts have focused on the genetic dissection of drought tolerance through identification of markers defining quantitative trait loci (QTL) with effects on specific traits related to drought tolerance. Others have studied the relationship of the drought response and yield components, morphological traits and physiological parameters. To our knowledge, QTLs with effects on drought tolerance have not yet been identified in cowpea. The main reason is that very few researchers are working on drought tolerance in cowpea. Some other reasons might be related to the complex nature of the drought stress response, and partly to the difficulties associated with reliable and reproducible measurements of a single trait linked to specific molecular markers to be used for marker assisted breeding. Despite the fact that extensive research has been conducted on the screening aspects for drought tolerance in cowpea only very few—like the ‘wooden box’ technique—have been successfully used to select parental genotypes exhibiting different mechanisms of drought tolerance. Field and pot testing of these genotypes demonstrated a close correspondence between drought tolerance at seedling and reproductive stages. Some researchers selected a variety of candidate genes and used differential screening methods to identify cDNAs from genes that may underlie different drought tolerance pathways in cowpea. Reverse genetic analysis still needs to be done to confirm the functions of these genes in cowpea. Understanding the genetics of drought tolerance and identification of DNA markers linked to QTLs, with a clear path towards localizing chromosomal regions or candidate genes involved in drought tolerance will help cowpea breeders to develop improved varieties that combine drought tolerance with other desired traits using marker assisted selection.
  相似文献   

14.
稻种资源苗期氮素营养效率的分类、鉴定与评价   总被引:5,自引:0,他引:5  
程建峰  戴廷波  曹卫星  姜东 《作物学报》2005,31(12):1640-1647
在低氮(20 mg/kg)、中氮(40 mg/kg)和高氮(60 mg/kg)3个水平下,调查了88份稻种资源的苗期性状并测定了氮素营养效率,包括氮素利用效率(NUE)、氮素吸收效率(NAE)和氮素利用效率响应度(NUER)。结果表明,苗期不同性状对低氮、中氮和高氮的敏感性不同,不同氮素水平间的单株根体积、地上部干重、吸氮量和根干重等性状变异较大,低氮胁迫加大了种质间的差异。不同水稻苗期性状对氮素的响应度不同,性状在种质间的变异服从正态分布。通过分类、 鉴定和评价,供试稻种资源分为13种氮素营养效率类型,它们存在明显的基因型差异,呈典型的正态分布,即低效和高效的较少,以NUE、NAE和NUER中效类型最多。NUE、NAE和NUER三指标间还存在很强的互补性和拮抗性,极少存在两个以上指标同为低效或高效的稻种资源类型。水稻氮素营养效率类型较陆稻丰富,但分布较分散,总体分布明显偏向于低氮素利用效率一侧。籼稻氮素营养效率的类型略多于粳稻,而粳稻类型的总体分布明显偏向于低氟素利用效率一侧。地方晶种氮素营养效亭类型较多、分布较集中,总体分布明显偏向于高氮素利用效率一侧:杂交稻氟素营养效率的类型最少、分布最集中,而常规稻氮素营养效率类型最多、分布较分散,常规稻和杂交稻品种的总体分布明显偏向于低氮素利用效率一侧。上述研究结果对探讨稻种资源蓖素营养效率的遗传特性、选育高效氮素营养的新型种质及阐明高效氮素营养的生理机制等具一定参考价值。  相似文献   

15.
This study aimed to identify whether and how sugarcane (Saccharum spp.) breeding in Argentina modified nitrogen‐use efficiency (NUE), water‐use efficiency (WUE) and radiation‐use efficiency (RUE). Thirteen varieties were grown in two consecutive seasons. Trends in different traits were estimated by fitting the data to linear or bilinear regression models. There was a linear increase in NUE and WUE with the year of release throughout the 70‐year span, whereas water use was not modified by sugarcane breeding. There was a positive and strong (r > 0.90; P < 0.01) association between NUE and WUE and between sugar yield and NUE or WUE. Although RUE was not modified by sugarcane breeding, the amount of radiation intercepted by the crop increased with the year of release. Modern varieties had a higher maximum interception and needed fewer days to reach maximum interception than old varieties. This study suggests that applying ecophysiological knowledge would be instrumental in sugarcane breeding programmes in order to develop varieties with high resource‐use efficiency and capable to adapt to global climate change.  相似文献   

16.
玉米氮效率的杂种优势分析   总被引:14,自引:0,他引:14  
陈范骏  米国华  春亮  刘建安  王艳  张福锁 《作物学报》2004,30(10):1014-1018
在氮高效自交系筛选工作的基础上,利用NCⅡ设计进行了杂种优势分析,同时探讨了氮高效育种中亲本选配等问题。结果表明:以农大108在两个氮水平下的产量为对照,18个自交系组配的72个杂交组合中,在高氮(200 kg N·hm-2)条件下有7个组合优势超过对照10%,在低氮(不施氮)条件下有5个组合优势超过对照10%,而且氮高效杂交组合  相似文献   

17.
水稻生理生化特性对氮肥的反应及与氮利用效率的关系   总被引:37,自引:6,他引:37  
选用水稻氮高效基因型IR72和9311及氮低效基因型Lemont和PECOS,采用土培方法,在5个施氮量(0、0.51、1.02、1.53、2.04 g N 钵-1,分别相当于0、75、150、225、300 kg N hm-2)处理下,研究了生理生化特性对氮肥的反应及与氮效率的关系。结果表明,在幼穗分化期,氮高效基因型水稻的可溶性蛋白含量相对低,而谷氨酰胺合成酶(GS)活性高;不同氮效率基因型间1,5-二磷酸核酮糖羧化酶/加氧酶(Rubisco)含量的差异不大;两种氮效率基因型间的净光合速率(Pn)在幼穗分化期差异不明显。而在齐穗期,氮低效基因型的Pn比高效基因型的低28.66%左右;氮低效基因型在两个时期的单位叶绿素光合速率(Pn/Chl)比氮高效基因型分别低18.51%和29.67%左右。在成熟期,氮高效基因型干物质积累能力强,籽粒产量高。这些结果说明氮效率不同的基因型对氮肥的生理反应差异大。相关性分析表明,低氮水平时(0~1.53 g N 钵-1), GS酶活性与收获时生物量呈显著或极显著正相关;氮肥偏生产力(PFP)、氮肥农学利用率(AE)及氮素生理利用率(NUEb)分别与GS活性、Pn/Chl和齐穗期的Pn呈显著正相关,而与可溶性蛋白含量、Rubisco含量显著负相关;氮肥吸收效率(RE)与这些生理指标没有显著相关。结果表明水稻光合特征及氮代谢与水稻氮效率间存在紧密的关系,GS活性和可溶性蛋白含量对评价水稻氮肥利用率具有重要的参考价值。  相似文献   

18.
中国作物新基因发掘:现状、挑战与展望   总被引:6,自引:0,他引:6  
中国作物新基因发掘是实现中国作物种质资源优势向基因资源优势转变和作物分子育种的基础。本文对中国近10年来水稻、小麦、玉米、大豆、棉花和油菜等主要作物基因发掘研究的进展进行了分析和评述。中国作物基因发掘也取得了一系列突破性进展:(1)创制出一批具有特色的基因发掘材料,包括基于中国作物遗传多样性的核心种质、基于优异资源的遗传分离群体和基于人工诱变的突变体等;(2)基因发掘技术和方法有所突破,尤其是在针对基因特点整合各种基因发掘技术、改进基因/QTL的生物统计算法等,提高了基因发掘的效率;(3)作物农艺性状的标记与基因定位已成为常规遗传研究方法,初步定位了一批抗病、抗逆、优质、养分高效、高产相关基因/QTL,其中,有500多个基因已精细定位;(4)以水稻为代表的作物基因克隆及功能研究在国际上受到瞩目,在主要作物中已克隆了300多个基因,其中,在目标作物中验证的基因数超过70个。在国际作物基因发掘高效化、规模化及实用化发展过程中,中国作物基因发掘也取得了重要进展。然而,中国作物基因发掘的数量和质量还远远不能满足作物分子育种的需求,与国际作物基因发掘也存在差距,具体表现为不同作物基因发掘研究进展不平衡、发掘基因的数量还相对有限、已发掘的基因中具有重大利用价值的基因不多。针对中国基因发掘面临的问题和世界各国以及跨国生物技术公司争夺基因的巨大挑战,作者提出了中国作物基因发掘应重点提高基因发掘效率,加强重要基因克隆及基因的价值评估,以生物产业发展需求为导向的基因发掘策略。  相似文献   

19.
Biological resources are the basic infrastructure of bioscience research. Rice (Oryza sativa L.) is a good experimental model for research in cereal crops and monocots and includes important genetic materials used in breeding. The availability of genetic materials, including mutants, is important for rice research. In addition, Oryza species are attractive to researchers for both finding useful genes for breeding and for understanding the mechanism of genome evolution that enables wild plants to adapt to their own habitats. NBRP-RICE contributes to rice research by promoting the usage of genetic materials, especially wild Oryza accessions and mutant lines. Our activity includes collection, preservation and distribution of those materials and the provision of basic information on them, such as morphological and physiological traits and genomic information. In this review paper, we introduce the activities of NBRP-RICE and our database, Oryzabase, which facilitates the access to NBRP-RICE resources and their genomic sequences as well as the current situation of wild Oryza genome sequencing efforts by NBRP-RICE and other institutes.  相似文献   

20.
Despite its high nitrogen absorption capacity, oilseed rape (OSR) has a low apparent nitrogen use efficiency (NUE), which makes its production highly dependent on nitrogen fertilization. Improving NUE in OSR is therefore a main target in breeding. The objectives of the present work were to determine the genomic regions (QTLs) associated with yield and to assess their stability under contrasted nitrogen nutrition regimes. One mapping population, AM, was tested in a French location for three growing seasons (2011, 2012 and 2013), under two nitrogen conditions (optimal and low). Eight yield-related traits were scored and nitrogen-responsive traits were calculated. A total of 104 QTLs were detected of which 28 controlled flowering time and 76 were related to yield and yield components. Very few genotype × nitrogen interactions were detected and the QTLs were highly stable between the nitrogen conditions. In contrast, only a few QTLs were stable across the years of the trial, suggesting a strong QTL × year interaction. Finally, eleven critical genomic regions that were stable across nitrogen conditions and/or trial years were identified. One particular region located on the A5 linkage group appears to be a promising candidate for marker assisted selection programs. The different strategies for OSR breeding using the QTLs found in the present study are discussed.  相似文献   

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