共查询到18条相似文献，搜索用时 625 毫秒
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玉米致死性坏死病是由玉米褪绿斑驳病毒(Maize chlorotic mottle virus,MCMV)和一种或多种马铃薯Y病毒科病毒复合侵染引起的。2014年1月在对云南省玉溪市玉米病毒病害的调查中发现了一些表现严重花叶、矮化叶片甚至整个植株坏死症状的玉米。对采集样品进行RT-PCR检测,所有样品中都同时检测到了MCMV和甘蔗花叶病毒(Sugarcane mosaic virus,SCMV),在一个样品中同时检测到了MCMV、SCMV和南方水稻黑条矮缩病毒(Southern rice black-streaked dwarf virus)。 相似文献
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玉米褪绿斑驳病毒实时荧光RT-PCR检测方法研究 总被引：2，自引：0，他引：2
玉米褪绿斑驳病毒(Maize chlorotic mottle virus,MCMV)是我国对外公布的检疫性有害生物。本研究根据该病毒外壳蛋白基因的保守序列,设计得到特异性引物及Taqman荧光探针,建立了MCMV的实时荧光RT-PCR方法,并对其灵敏度与特异性进行了研究。该方法针对2个不同来源的毒株均能得到典型扩增曲线,而没有从小麦线条花叶病毒、玉米粗缩病毒和玉米矮花叶病毒的RNA得到扩增曲线,表明引物与荧光探针具有良好的特异性。针对玉米褪绿斑驳病毒RNA不同稀释度样品,实时荧光RT-PCR检测低限达到10-5稀释度,检测灵敏度要比普通RT-PCR高出100倍。因此,本研究建立的MCMV实时荧光方法具有特异性强、灵敏度高和快速有效的优点。 相似文献
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玉米褪绿斑驳病毒(Maize chlorotic mottle virus,MCMV)是我国进境植物检疫性有害生物,与马铃薯Y病毒科的部分病毒复合侵染导致玉米致死性坏死病。利用Maxent模型,分析预测了MCMV在当前和未来气候条件下的潜在地理分布区。结果显示,MCMV在全球的适生区主要集中在大洋洲东部局部地区,亚洲、非洲、北美洲的南部地区,南美洲的西北和东北部地区;在我国的适生区范围主要集中在中部和南部地区。根据预测结果,提出对MCMV的防控措施建议。 相似文献
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重组酶聚合酶扩增技术(RPA)快速检测玉米褪绿斑驳病毒 总被引：5，自引：3，他引：2
玉米褪绿斑驳病毒(maize chlorotic mottle virus,MCMV)是我国颁布的一种禁止进境的检疫性有害生物,自然寄主有玉米、小麦、黍、大麦等。自然情况下,MCMV常与甘蔗花叶病毒(sugarcane mosaic virus,SCMV)、玉米矮花叶病毒(maize dwarf mosaic virus,MDMV)、小麦线条花叶病毒(wheat streak mosaic virus,WSMV)共同侵染玉米,可造成玉米产量损失高达10%~90%(Hilker et al.,2017)。 相似文献
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为探讨烟草脆裂病毒（tobacco rattle virus,TRV）载体介导的基因沉默技术对朱顶红褪绿环斑病毒（Hippeastrum chlorotic ringspot virus,HCRV）运动蛋白基因NSm的沉默效应,以本氏烟Nicotiana tabacum为材料,构建靶向HCRV NSm序列的基因沉默表达载体pTRV2-NSm,经农杆菌Agrobacterium介导侵染烟苗,检测其侵染效率,人工接种HCRV到沉默表达载体处理过的烟苗,通过观察发病症状、统计病情并应用实时荧光定量PCR(quantitative real-time PCR,qRT-PCR)技术检测TRV介导的基因沉默体系对HCRV侵染的沉默效应。结果表明,pTRV2-NSm沉默表达载体对本氏烟植株的侵染率达到95.00%,并且对本氏烟的生长无明显影响;与对照相比,经沉默载体处理的烟草植株接种HCRV后发病率显著降低,在接种后14 d发病率下降了90个百分点,病毒积累量明显下降,随着时间延长pTRV2-NSm对病毒的抑制效果持续升高,在接种后14 d对病毒病的防治效果最高,达93.13%;qRT-PCR检测发... 相似文献
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玉米是我国重要的粮食作物、饲料和工业原材料。随着我国粮食贸易量增加,病原物入侵的风险也随之加大。该文对4种主要的检疫性玉米病毒——玉米褪绿斑驳病毒 Maize chlorotic mottle machlomovirus、玉米矮花叶病毒 Maize dwarf mosaic potyvirus、小麦线条花叶病毒 Wheat streakmosaic tritimovirus和玉米褪绿矮缩病毒 Maize chlorotic dwarf waikavirus的生物学特性及其危害与分布情况进行归纳总结,同时对这些病毒致病与寄主抗病的相关机制以及检测方法的研究进展进行介绍,并针对检疫性玉米病毒的防控,在快速检测体系的建立与抗病毒种质的创制方面提出展望。 相似文献
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为明确玉米褪绿斑驳病毒 （maize chlorotic mottle virus, MCMV）对我国玉米生产的经济损失,通过收集、整理玉米产量、种植面积、市场价格以及MCMV潜在地理分布、危害和防控等相关数据,基于随机模型利用@RISK软件分别预测MCMV在不防控和防控场景下对我国玉米产业造成的潜在经济损失。结果表明,在不防控场景下, MCMV对我国玉米产业造成的潜在经济损失总量的90%置信区间为329.44亿~508.96亿元;而在防控场景下, MCMV对我国玉米产业造成的潜在经济损失总量的90%置信区间为51.62亿~76.23亿元,可挽回潜在经济损失的90%置信区间为278.38亿~437.93亿元。说明MCMV严重威胁我国玉米生产,建议有关部门加强检疫阻截防控工作,保障我国玉米产业安全。 相似文献
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RNA干扰被认为是转录后基因沉默的一种机制。RNA干扰通过小干扰RNA特异性降解目标mRNA来沉默基因表达。本文以烟草花叶病毒126kD蛋白为靶蛋白,在原生质体水平上研究了小干扰RNA对病毒侵染的干扰和抑制作用。ELISA和Northern杂交的实验结果表明,共转染小干扰RNA和TMV的原生质体内检测到较低的病毒含量。在枯斑寄主上,叶片接种小干扰RNA和TMV共转染原生质体后,与对照叶片相比,仅有很少量的病斑产生。这说明,小干扰RNA能够在原生质体水平对病毒起到干扰和抑制作用。因此认为,烟草原生质体系统有利于快速和定量分析小干扰RNA介导对植物病毒的抑制作用。 相似文献
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Yan Shi Yanhong Qin Yanyong Cao Hu Sun Tao Zhou Yiguo Hong Zaifeng Fan 《European journal of plant pathology / European Foundation for Plant Pathology》2011,131(2):317-326
Expression of many host genes can be altered during virus infection. In a previous study of sugarcane mosaic virus (SCMV)
infection in maize (Zea mays), we observed that expression of ZmTrm2, a gene encoding thioredoxin m, was up-regulated at about 10 days post-inoculation (dpi). In this present study we determined that ZmTrm2 silencing in maize by virus-induced gene silencing significantly enhanced systemic SCMV infection. In contrast transient
over-expression of ZmTrm2 in maize protoplasts inhibited accumulation of SCMV viral RNA. Furthermore, we found that in inoculated Nicotiana tabacum leaves transient expression of ZmTrm2 inhibited accumulation of the RNA of tobacco vein-banding mosaic virus (TVBMV), a potyvirus infecting dicotyledonous plants.
Interestingly in ZmTrm2 transiently expressed N. tabacum leaves, we detected by semi-quantitative RT-PCR a reduced level of the mRNA of class I beta-1, 3-glucanase (GluI), a protein known to have a role in cell wall callose deposition and viral movement. Our data indicate that the maize ZmTrm2 plays an inhibitory role during infection of plants by SCMV and TVBMV. 相似文献
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永安地区发病辣椒植株表现小叶、黄化、丛枝、簇芽等症状。利用植原体16SrDNA基因的通用引物R16mF2/R16mR2和R16F2n/R16R2,对发病辣椒植株总DNA进行巢式PCR检测,获得约1.2kb的特异性DNA片段。经测序并在GenBank数据库进行比对分析,共获得4条植原体特定的16SrDNA基因序列(CHY-C4-1、CHYY1-1、CHY-Y7-1、CHY-G1-1)。将测得的4条序列与已报道的植原体序列进行同源性比对,并构建系统进化树,结果显示获得的4条植原体序列均聚类到16SrI组,其中CHY-Y1-1、CHY-Y7-1、CHY-G1-1与16SrI-B亚组植原体聚类到同一支,而CHY-C4-1与已报道的16SrI组内的6个亚组均未聚类到一支,因此建议将CHY-C4-1命名为新的亚组。利用iPhyClassifier在线分析软件对获得的4条植原体序列进行虚拟RFLP分析,结果与进化树获得的结果一致。 相似文献
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D. F. Quito-Avila R. A. Alvarez A. A. Mendoza 《European journal of plant pathology / European Foundation for Plant Pathology》2016,146(3):705-710
Maize plants displaying severe mosaic, chlorosis and, in some cases, necrosis were observed in two provinces of Ecuador. Shotgun sequencing of cDNA obtained from double-stranded RNA extracted from symptomatic samples revealed the presence of Sugarcane mosaic virus (SCMV) and Maize chlorotic mottle virus (MCMV), two components of maize lethal necrosis. In 2015, a survey conducted in Los Ríos, Manabí and Guayas, major maize production areas in the country, showed that the two viruses were present in a significant number of plants. Mixed infections were detected and associated with severe symptoms. MCMV was also detected in two hybrid seed lots, a significant finding for the epidemiology of the disease in Ecuador. The report of maize lethal necrosis, today the most significant virus disease of maize, in Ecuador is of great concern for producers in the South American continent because of the potential of an epidemic as that observed in east Africa in recent years. 相似文献
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进境玉米种子携带玉米褪绿斑驳病毒的检测与鉴定 总被引：3，自引：0，他引：3
Imported maize seeds were planted in a quarantine greenhouse and two seedlings with chlorotic mottle symptoms were observed. The ELISA results showed that the two seedlings reacted positively with antibody against Maize chlorotic mottle virus (MCMV). The positive samples were further identified by RT-PCR method and the 711 bp size target bands were amplified specifically. The similarity range of nucleotide sequence of both RT-PCR product and six MCMV coat protein genes was 97%-99%. The amino acid sequence homology was 97.88%-99.89%. Based on the above results, the virus was identified as MCMV. It was the first time that MCMV was intercepted from imported maize seeds. 相似文献
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Effect of temperature on RNA silencing of a negative‐stranded RNA plant virus: Citrus psorosis virus
K. Velázquez A. Renovell M. Comellas P. Serra M. L. García J. A. Pina L. Navarro P. Moreno J. Guerri 《Plant pathology》2010,59(5):982-990
Citrus psorosis virus (CPsV), genus Ophiovirus, causes a bark scaling disease of citrus. CPsV virions are kinked filaments with three negative‐stranded RNA molecules (vRNA) and a 48 kDa coat protein. The effect of temperature on symptom expression, virus accumulation and RNA silencing was examined in sweet orange seedlings (Citrus sinensis) graft‐inoculated with three different CPsV isolates and grown in a glasshouse at 26/18°C or 32/26°C (day/night). Most plants kept in the cooler glasshouse showed a shock reaction in the first flush with shoot necrosis, and then moderate to intense chlorotic flecking and spotting in young leaves, whereas plants incubated at 32/26°C did not exhibit shoot necrosis, and young leaf symptoms were milder. Virus titre estimated by ELISA and by northern and dot blot hybridization paralleled symptom intensity, with significantly higher virus accumulation in plants incubated at 26/18°C. The amount of CPsV‐derived small RNAs (CPsV‐sRNAs) slightly increased at 32/26°C, with the ratio of CPsV‐sRNA/vRNA being higher at 32/26°C than at 26/18°C. These results suggest that (i) CPsV infection induces RNA silencing in citrus plants, (ii) symptom intensity is associated with virus accumulation, and (iii) temperature increase enhances the RNA silencing response of citrus plants and decreases virus accumulation. 相似文献
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