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1.
Bovine brucellosis, caused by Brucella
abortus, is a significant problem for both public and animal health in Turkey. This study was conducted on the calving seasons between
2001 and 2006. A total of 626 serum samples of cattle obtained from 27 herds with a history of abortions was examined for
Brucella antibodies by RBPT, SAT and ELISA. Of the cattle sera analysed, 221 (35,30%) and 206 (32, 92%) and 247 (39,45%) were found
to be positive by RBPT , SAT and ELISA, respectively. B. abortus was isolated from 48 (32,21%) of 149 lung samples and stomach contents of the aborted fetuses. Based on the biochemical tests
and the agglutination tests with monospecific A and M antisera, only 3 of the isolates were found to be B. abortus biotype 1 and the remaining 45 were biotype 3. This study also revealed that the dominant biotype of B. abortus was biotype 3 in this region. The determination of the agents responsible for bovine brucellosis and serosurvey of this disease
are expected to help better understanding of this zoonotic infection in this region and neighbouring countries. 相似文献
2.
Qiwei Chen Xiaowei Gong Fuying Zheng Xiaoan Cao Zhaocai Li Jizhang Zhou 《Tropical animal health and production》2014,46(3):503-507
Chlamydophila abortus is an important amphixenosis which in a wide range of animals, associated with reproductive disorders in yaks. In order to assess the prevalence of this infection in yaks in Qinghai, China, a cross-sectional study was carried out, and a total of 674 serum samples were collected from June to October 2012 in six counties, and antibodies to C. abortus were examined by indirect hemagglutination (IHA) test. The overall seroprevalence of C. abortus in yaks was 17.66 % (119/674), and the seroprevalence of antibodies to C. abortus in yaks ranged from 11.82 to 28.43 % among the six different areas, and the difference was statistically significant (P?<?0.05). The seropositivity of C. abortus infection in different age groups varied from 16.33 to 18.49 %, and prevalence in yaks of ≥3 year (18.49 %) was slightly higher than that in yaks of <3 year, but the differences among the age groups were not statistically significant (P?>?0.05). The seroprevalence of C. abortus infection in male yak (16.8 %) was slightly lower than that in females (17.85 %), and the difference was not statistically significant (P?>?0.05). So far, this is the first systematic and comprehensive investigation of C. abortus infectionin in yaks in this area. 相似文献
3.
Studies conducted on the seroprevalence of brucellosis in 98 mithuns maintained at the National Research Centre on Mithun, Nagaland, India revealed that the number of animals found positive for brucellosis in avidin-biotin enzyme-linked-immunosorbent assay, standard tube-agglutination test and Rose-Bengal plate test were 34, 20 and 11%, respectively. Highest prevalence of brucellosis was observed in the Mizoram mithun strain. The relative sensitivity and specificity of the standard tube-agglutination test were 61 and 100%, respectively; the corresponding values of the Rose-Bengal plate test were 33 and 100%, respectively. 相似文献
4.
A comparison of standard serological tests for the diagnosis of bovine brucellosis in Canada. 总被引：1，自引：1，他引：0 
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下载免费PDF全文 B W Stemshorn L B Forbes M D Eaglesome K H Nielsen F J Robertson B S Samagh 《Canadian journal of veterinary research》1985,49(4):391-394
Six agglutination and two complement fixation tests were compared with respect to specificity, sensitivity and relative sensitivity for the serodiagnosis of bovine brucellosis. Based on 1051 sera from brucellosis free herds, the specificity of the tests was 98.9% for the buffered plate antigen test (BPAT), 99.2% and 99.3% for the standard tube and plate agglutination tests (STAT and SPAT), respectively, and 99.8% for the 2-mercaptoethanol test (2MET). On this small sample, the rose bengal plate test (RBPT), card test (CARD) and the complement fixation test (CFT) correctly classed all sera as negative. On a sample of 167 culture positive cattle, the sensitivities of the tests were CFT: 79.0%, BPAT: 75.4, RBPT: 74.9%, CARD: 74.3%, SPAT: 73.1%, STAT: 68.9%, and 2MET: 59.9%. All tests combined detected only 82% of these infected cattle. Analysis of the relative sensitivity of the six agglutination tests gave the following ranking: BPAT greater than RBPT greater than CARD greater than SPAT greater than STAT. The 2MET ranked between the BPAT and RBPT or between the RBPT and CARD depending on the analysis used. The use of the BPAT as a screening test is recommended provided that a test of high specificity and sensitivity such as the CFT is used to confirm screening test reactions. 相似文献
5.
M.D.J. Willet B.V.Sc. M.Ag.Sc. R.D. 《New Zealand veterinary journal》2013,61(3):42-43
Brucella abortus strain 19 vaccine became widely available in New Zealand in 1950. In 1966, vaccination was made compulsory and age restrictions were imposed. Surveys of the cattle population were carried out between 1966 and 1970 to establish the prevalence of brucellosis and to compare the performance of various diagnostic tests under New Zealand conditions. In 1969 and 1970 a voluntary test-and-slaughter scheme was introduced basedon the brucellosis card test as a screening test, the complement fixation test as the definitive test, and the milk ring test to screen accredited dairy herds. This scheme was extended to a compulsory national Brucellosis Eradication Scheme in September 1971, with the aim of bringing all eligible cattle under test by 1977. 相似文献
6.
Bovine herpesvirus-1 (BoHV-1) causes a variety of disease syndromes including, respiratory, nervous and reproductive disorders
both in domestic as well as wild bovines and the disease is prevalent throughout the world including India. In this study,
serum samples of yaks were screened for serological evidence of BoHV-1 in a yak farm with history of abortion and kerato-conjuctivitis
by competition-ELISA. The result of seroprevalence of BoHV-1 infections in yaks (Poephagus grunniens) revealed that the overall seroprevalence was 60.1%. The sero-prevalence of BoHV-1 infections was highest in male calf (67.7%)
followed by yak cows (62.6%), yak bulls (56.8%), and yak heifers (50.0%). 相似文献
7.
S. Bandyopadhyay P. Pal D. Bhattacharya A. K. Bera D. Pan H. Rahman 《Tropical animal health and production》2010,42(1):119-121
Faecal samples were collected from 348 yaks(Bos poephagus) in and around Gurudogmer Plateau, a cold desert area in North Sikkim, India. The prevalence of gastrointestinal parasitic
infection was recorded at 10.05% in both the organized and traditionally managed farms of yak. The pattern of infection was
either single (2.58%) or mixed (7.47%) with a faecal egg count range of 100–200 eggs per gram of faeces in positive animals.
Among the helminths, Haemonchus spp. infection was predominant (6.89%) followed by Nematodirus spp. (1.72%), Cooperia spp. (1.43%) and Dicrocoelium spp. (0.29%). 相似文献
8.
SUMMARY A group of 4 cows was vaccinated with Brucella abortus strain 19, followed 8 weeks later by a single dose of B. abortus 45/20 vaccine. A similar group received 2 doses of B. abortus 45/20 vaccine 8 weeks apart. The antibody responses of the groups were compared by testing whole serums and separated IgM and IgG fractions by the Rose Bengal Plate (RBP) agglutination and the complement fixation tests (CFT) using rough and smooth B. abortus antigens. Animals that had received B. abortus strain 19 responded to the 45/20 vaccine with increased titres to the smooth antigen. These relevant antibodies were predominantly of the IgG class. Standard CFT and RBP test antibodies could be detected in IgM and IgG fractions after the primary inoculation with B. abortus strain 19 vaccine. 相似文献
9.
Md. Ariful Islam Mst. Minara Khatun Byeong-Kirl Baek Sung-Il Lee 《Journal of veterinary science (Suw?n-si, Korea)》2009,10(3):211-218
Immunizing animals in the wild against Brucella (B.) abortus is essential to control bovine brucellosis because cattle can get the disease through close contact with infected wildlife. The aim of this experiment was to evaluate the effectiveness of the B. abortus strain RB51 vaccine in protecting infection as well as vertical transmission in Sprague-Dawley (SD) rats against B. abortus biotype 1. Virgin female SD rats (n = 48) two months of age were divided into two groups: one group (n = 24) received RB51 vaccine intraperitoneally with 3 × 1010 colony forming units (CFU) and the other group (n = 24) was used as non-vaccinated control. Non-vaccinated and RB51-vaccinated rats were challenged with 1.5 × 109 CFU of virulent B. abortus biotype 1 six weeks after vaccination. Three weeks after challenge, all rats were bred. Verification of RB51-vaccine induced protection in SD rats was determined by bacteriological, serological and molecular screening of maternal and fetal tissues at necropsy. The RB51 vaccine elicited 81.25% protection in SD rats against infection with B. abortus biotype 1. Offspring from rats vaccinated with RB51 had a decreased (p < 0.05) prevalence of vertical transmission of B. abortus biotype 1 compared to the offspring from non-vaccinated rats (20.23% and 87.50%, respectively). This is the first report of RB51 vaccination efficacy against the vertical transmission of B. abortus in the SD rat model. 相似文献
10.
Sandip Kumar Khurana Anju Sehrawat Ruchi Tiwari Minakshi Prasad Baldev Gulati Muhammad Zubair Shabbir Rajesh Chhabra Kumaragurubaran Karthik Shailesh Kumar Patel Mamta Pathak Mohd. Iqbal Yatoo Vivek Kumar Gupta Kuldeep Dhama Ranjit Sah Wanpen Chaicumpa 《The Veterinary quarterly》2021,41(1):61
Brucellosis is a zoonotic disease of great animal welfare and economic implications worldwide known since ancient times. The emergence of brucellosis in new areas as well as transmission of brucellosis from wild and domestic animals is of great significance in terms of new epidemiological dimensions. Brucellosis poses a major public health threat by the consumption of non-pasteurized milk and milk products produced by unhygienic dairy farms in endemic areas. Regular and meticulous surveillance is essentially required to determine the true picture of brucellosis especially in areas with continuous high prevalence. Additionally, international migration of humans, animals and trade of animal products has created a challenge for disease spread and diagnosis in non-endemic areas. Isolation and identification remain the gold standard test, which requires expertise. The advancement in diagnostic strategies coupled with screening of newly introduced animals is warranted to control the disease. Of note, the diagnostic value of miRNAs for appropriate detection of B. abortus infection has been shown. The most widely used vaccine strains to protect against Brucella infection and related abortions in cattle are strain 19 and RB51. Moreover, it is very important to note that no vaccine, which is highly protective, safe and effective is available either for bovines or human beings. Research results encourage the use of bacteriophage lysates in treatment of bovine brucellosis. One Health approach can aid in control of this disease, both in animals and man. 相似文献
11.
[目的]为了检验S19号疫苗的预防效果。[方法]用S19号疫苗对未孕母牛进行接种,然后用琥红平板凝集试验监测。[结果]不同个体免后4～5个月的转阳率55.8%,免后6～8个月的转阳率63.5%,免后11～12个月的转阳率57.5%,同一养殖区内注苗后的转阳率平均57.7%,未注苗的自然感染率达到16.2。[结论]说明疫... 相似文献
12.
Seroprevalence and associated risk factors of bovine brucellosis in Addis Ababa dairy farms 总被引：1，自引：1，他引：0
A cross-sectional study was carried out on bovine brucellosis in Addis Ababa dairy farms from November 2003 to April 2004. A total of 1,202 blood samples were collected from non-vaccinated, cross-bred dairy cattle. The Rose Bengal plate test (RBPT) was used as a screening test. Those serum samples reacting positively to RBPT were subjected to the complement fixation test (CFT) for confirmation. The RBPT detected 30 of 1,202 (2.5%) of the samples as brucellosis positive. The positive sera when further retested using CFT, 18 out of the 30 RBPT positive sera were confirmed to be positive. The prevalence of brucellosis based on CFT in the study area was 1.5%, and all positive sera were from female cattle. Result of the questionnaire survey revealed that percentage of 4.4% abortion and 9.5% retained fetal membranes. Abortion and retained fetal membranes were associated with Brucella antibodies (P?相似文献
13.
牦牛和犏牛促卵泡素受体基因5′-侧翼区序列多态性研究 总被引：1，自引：1，他引：0
本研究旨在分析牦牛和犏牛促卵泡素受体(follicle-stimulating hormone receptor,FSHR)基因的多态性,为从遗传角度上解决其繁殖产仔率低的问题提供参考,为筛选繁殖性状分子标记奠定理论基础。研究采用PCRSSCP和直接测序技术,对麦洼牦牛、九龙牦牛、大通牦牛和犏牛共110头个体的FSHR基因5′-侧翼区进行遗传多态性分析,统计基因频率和基因型频率,进行Hardy-Weinberg平衡性检测,计算纯合度、杂合度、多态信息含量和有效等位基因数等遗传多态性指标。结果表明,麦洼牦牛、大通牦牛和九龙牦牛FSHR基因5′-侧翼区核苷酸序列具有多态性,犏牛无多态性;麦洼牦牛存在AA、AB和BB 3种基因型,九龙牦牛和大通牦牛均存在AA、AB 2种基因型,AB基因型在3个牦牛品种中占绝对优势,等位基因A为优势等位基因;麦洼牦牛、九龙牦牛和大通牦牛的多态信息含量分别为0.3693、0.3565、0.3705,均达到了中度多态(0.25PIC0.5),表明各牦牛品种遗传变异较大。 相似文献
14.
Selecting 6 heads of new yak strains(the offspring of reciprocal cross of F1 produced by wild yak male mated with domestic yak female)and 6 domestic yaks at 3 and 12 age in months respectively at Datong Yak Farm of Qinghai Province. This paper was studied for the morphological characteristics of the skin and hair coat of yak and their adaptability to the cold living environment in Qinghai-Tibetan plateau. The results indicated that it should be of vital importance marker for the morphological characteristics of the skin and hair coat with two type of yaks at 3 and 12 age in months and their regulations of growth and development to adapt to the cold living environment in Qinghai-Tibetan plateau,The main morphological indexes of the skin and hair coat of the rearing new yak strain were higher than those of the domestic yaks at the same age in months, or speak precisely, the new yak strain was more powerful adaptable to the alpine cold living environment than that of the domestic yaks. The resvlts above provided scientific basis for the native strain breeding of yak and the new yak strain rearing. 相似文献
15.
Brucellosis is a highly contagious zoonotic infection affecting livestock and human beings. The disease has been reported worldwide except in few countries where it has been eradicated. The prevalence of brucellosis among cattle from 11 farms having a history of abortions was studied. A total of 481 samples comprising of blood, milk, vaginal swabs, vaginal discharges, placental tissues and fetal tissues were collected from 296 animals. Clinical samples were processed for the isolation of Brucella. Serum samples (n = 296) were tested by Rose Bengal Plate Test (RBPT) and indirect ELISA. A total of 90 (30.40%) and 123 (41.55%) samples were positive by RBPT and indirect ELISA, respectively. Also 27.02% samples were positive by both the tests. Brucella isolates (n = 8) were recovered from clinical samples using Brucella selective media. All the isolates demonstrated PCR amplification for the bcsp31 and IS711 genes. Amplification of Brucella abortus specific primer was demonstrated by all the isolates in AMOS PCR indicating isolates to be of either B. abortus biotype 1, 2 or 4. Risk factors for transmission of brucellosis among cattle population were studied by field surveys. It was observed that lack of awareness about brucellosis (OR = 8.739, P = 0.138) and inadequate floor space (OR = 0.278, P = 0.128) were crucial risk factors for transmission of bovine brucellosis. 相似文献
16.
Fosgate GT Diptee MD Ramnanan A Adesiyun AA 《Tropical animal health and production》2011,43(8):1479-1486
The water buffalo is an important domestic animal worldwide, and the local Buffalypso variety was developed in Trinidad to
have improved beef qualities. Brucellosis was diagnosed in Trinidad and Tobago during 1998 in both cattle and domestic water
buffalo (Bubalus bubalis) populations. Brucellosis in the latter species is caused by infection with Brucella abortus, similar to bovine brucellosis. Control of brucellosis is of paramount importance to preservation of the genetic diversity
of these animals in Trinidad, and this has been complicated by differences in the epidemiology of water buffalo and bovine
brucellosis. Some diagnostic tests do not have comparable accuracy between the two species, and the RB51 vaccine does not
adequately protect against infection in water buffalo. The water buffalo in Trinidad may also be more resistant to infection
than cattle. Development of effective vaccination protocols is key to brucellosis control in Buffalypso in Trinidad, and prohibitions
on import of virulent B. abortus strains for vaccine efficacy studies has impeded progress in this area. These Trinidadian strains are of variable virulence;
some might be effective for challenge in vaccine efficacy studies, while other, of lower virulence, may be vaccine candidates
for use in water buffalo. 相似文献
17.
A. A. ADESIYUN S. U. ABDULLAHI J. B. ADEYANJU 《The Journal of small animal practice》1986,27(1):31-37
Serum samples collected from dogs brought for routine physical examination, vaccination and other complaints at the Small Animal Clinic of Ahmadu Bello University, Zaria, Nigeria were tested for Brucella abortus and Brucella canis antibodies. Ninety-five (38-2 per cent) of 249 dogs studied were positive for B. abortus agglutinins by the Rose Bengal plate test (RBPT) but none was sero-positive by the standard agglutination test (SAT). The antibody prevalence for B. canis by the SAT was 28-6 per cent for 224 dogs tested. Exotic breeds of dogs had a prevalence of 34-9 per cent for B. canis agglutinins while 28-1 per cent of local dogs were sero-positive. Twenty-two per cent of dogs older than 2 years were sero-positive compared to a prevalence of 33-3 per cent found amongst dogs younger than 1 year. A similar B. canis infection rate was observed amongst male (29-6 per cent) and female (26-7 per cent) dogs. 相似文献
18.
牦牛病毒性腹泻／粘膜病防制中间试验 总被引：6，自引：0，他引：6
从１９９１￣１９９３年，应用牛病毒性腹泻／粘膜病（ＢＶＤ／ＭＤ）Ｏ系弱毒冻干苗６０万头份对我省青南地区玉树、称多、泽库三个ＢＶＤ／ＭＤ发病严重的县进行了大面积的预防注射，调者被注射牛７６７９７头；同时在该地区应用猪瘟弱毒苗预防注射牛２０万头份，调查被注射牛４８０００头。试验结果：应用ＢＶＤ／ＭＤ Ｏ系弱毒冻干苗使牦牛ＢＶＤ／ＭＤ死亡率由６．６１％降至０．２４％，应用猪瘟弱毒苗亦对该病有较好的防制效 相似文献
19.
为查明甘肃省甘南玛曲县牦牛衣原体感染情况并分析影响其感染的因素,试验采用间接血凝试验(IHA)方法检测了该地区的610份牦牛血清,并应用流行病学、统计学方法对影响牦牛衣原体的因素进行了分析。结果表明,牦牛衣原体抗体阳性率为25.08%,季节、性别和年龄对牦牛衣原体感染的影响具有显著差异(P<0.05),胎次对母牦牛衣原体感染的影响差异显著(P<0.05)。由此可知,甘肃省甘南玛曲地区牦牛衣原体感染率较高,因此应对本地区的衣原体病采取适当的控制措施,以确保人类的安全。 相似文献
20.
U.S Salisu C.A Kudi J.O.O. Bale M Babashani B.Y Kaltungo S.N.A Saidu A Asambe A.Y Baba 《Tropical animal health and production》2017,49(5):1041-1046
A cross-sectional study was carried out to determine the status of Brucella infection in one-humped (Dromedary) camels in the North and Central senatorial districts of Katsina State, Nigeria. Nine hundred and eighty serum samples from live and slaughtered camels were tested. Modified Rose Bengal plate test (RBPT) and serum agglutination test (SAT) with ethylenediaminetetraacetic acid, (EDTA) were used as screening and standard tests, respectively. The prevalence of Brucella antibodies were 110 (11.2%) and 103 (10.5%) for RBPT and SAT, respectively. Of the 472 and 508 serum samples tested from the herds and abattoirs, respectively, 63 (13.3%) and 47 (9.3%) were positive by RBPT while 62 (13.1%) and 41 (8.1%) were positive by SAT, respectively. Based on the results, it was concluded that Brucella antibodies were present in camels in the study area. Poor management practices and mixing of camels with other species of livestock as well as unrestricted movement of camels were proposed to be the reasons for the prevalence of the disease in the study area. In view of the public health importance of the disease, it is recommended that there is the need to develop a strategic plan to decrease spread of brucellosis in the study area. 相似文献