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1.
为了研究马齿苋(Portulaca oleracea L.)多糖(PHP)抗肿瘤作用的免疫学机制,试验建立荷U14宫颈癌实体瘤小鼠模型,将荷瘤小鼠随机分为荷瘤对照组、阳性对照组(CTX)、PHP低剂量组、PHP高剂量组,连续给药13 d后,解剖取胸腺和脾脏,测定胸腺指数和脾脏指数,应用碳粒廓清试验测定PHP对荷瘤小鼠免疫吞噬功能的影响;Con A诱导荷瘤小鼠脾淋巴细胞转化试验检测PHP对荷瘤小鼠机体细胞免疫功能的影响;乳酸脱氢酶释放法检测PHP对荷瘤小鼠非特异性免疫功能的影响;血清溶血素测定法检测PHP对荷瘤小鼠体液免疫功能的影响。结果表明:PHP可明显提高荷瘤小鼠免疫器官重量及增强荷瘤小鼠细胞免疫及体液免疫功能。说明PHP抗肿瘤作用的机制可能与提高机体免疫功能有关。  相似文献   

2.
目的研究番茄红素的体内抗肿瘤作用.方法通过S180肉瘤移植建立荷瘤小鼠模型,给予番茄红素后观察荷瘤小鼠肿瘤生长情况及对小鼠免疫系统和抗氧化酶功能的影响.结果番茄红素具有明显的抗肿瘤作用,可增强小鼠脾脏淋巴细胞增殖能力;提高自然杀伤细胞活性;明显提高CD4 和CD8 T细胞数量及其比值;可提高小鼠血清SOD、GSHPx活性,降低MDA含量.结论番茄红素明显的抑制肿瘤生长的作用,其作用可能与机体免疫系统功能的增强和抗氧化酶活力的提高有关.  相似文献   

3.
用不同剂量的旋毛虫感染BALB/c小鼠,并将试验组分为荷瘤11 d前接种旋毛虫组和荷瘤11 d后接种旋毛虫组,2组均于荷瘤20 d后解剖小鼠,测定小鼠体内肿瘤的体积、重量及抑瘤率,并检测T淋巴细胞亚群。试验结果表明旋毛虫对BALB/c小鼠体内的MCF-7肿瘤细胞的生长有一定的抑制作用,并且荷瘤11 d前接种旋毛虫组的抑瘤效果优于荷瘤11 d后接种旋毛虫组。  相似文献   

4.
目的研究番茄红素的体内抗肿瘤作用。方法通过S180肉瘤移植建立荷瘤小鼠模型，给予番茄红素后观察荷瘤小鼠肿瘤生长情况及对小鼠免疫系统和抗氧化酶功能的影响。结果番茄红素具有明显的抗肿瘤作用，可增强小鼠脾脏淋巴细胞增殖能力；提高自然杀伤细胞活性；明显提高CD^4＋和CD^8＋T细胞数量及其比值；可提高小鼠血清SOD、GSHPx活性，降低MDA含量。结论番茄红素明显的抑制肿瘤生长的作用，其作用可能与机体免疫系统功能的增强和抗氧化酶活力的提高有关。  相似文献   

5.
《中国兽医学报》2015,(12):1921-1927
为评价重组新城疫病毒(NDV)血凝素-神经氨酸酶(HN)基因植物乳杆菌对鼠结肠癌(CRC)潜在的抑制作用,试验以前期构建的可表达NDV HN基因的植物乳杆菌(简称重组菌HN/Lab)为研究对象,通过肿瘤诱导建立小鼠CRC皮下移植瘤模型,通过腹部手术建立小鼠原位移植瘤模型,并在肿瘤诱导前后给荷瘤鼠灌胃重组菌和不表达HN基因的植物乳杆菌(L.p),通过监测肿瘤体积、荷瘤鼠的存活率以及肿瘤组织的病理学来评价重组菌对CRC的抑制作用。结果表明,试验分别成功建立了小鼠CRC皮下移植瘤和原位移植瘤模型;重组菌(CT26+HN/Lab组)能明显抑制荷瘤鼠皮下肿瘤的生长,分别在肿瘤诱导后25,30 d差异显著(P0.05),重组菌组小鼠的存活率和生存时间也显著高于CT26组(P0.05);重组菌对小鼠原位移植瘤模型抑制效果明显,在肿瘤诱导7 d后即能明显抑制肿瘤的生长(P0.05),抑瘤率为43.13%,与植物乳杆菌(CT26+L.p组)23.03%抑瘤率相比有了明显的提高;病理组织学观察结果显示,重组菌诱导肿瘤坏死明显,浸润的淋巴细胞数量增多,能够抑制肿瘤细胞向黏膜层转移。因此,NDV HN基因重组乳酸菌对鼠CRC模型具有较好的抑制作用。  相似文献   

6.
为了研究鄂尔多斯高原碱湖钝顶螺旋藻多糖的抗肿瘤作用和免疫作用,试验采用S180腹水瘤模型,观察鄂尔多斯高原碱湖钝顶螺旋藻多糖不同剂量组小鼠的生存时间、生存状况、肿瘤分布及瘤体外观,测定多糖对小鼠免疫器官指数的影响。结果表明:高、中、低3个剂量的钝顶螺旋藻多糖均能改善腹水瘤小鼠的生存状况,延长小鼠的生存时间,缩小肿瘤在体内的扩散范围;不同剂量组小鼠胸腺指数和脾脏指数均高于荷瘤对照组,以中剂量组作用效果最为显著(P0.05),3个剂量组之间差异均不显著。说明鄂尔多斯高原碱湖钝顶螺旋藻多糖能够抑制肿瘤在小鼠体内的生长与扩散,改善肿瘤小鼠的生存质量,同时促进小鼠免疫器官的生长发育,从而增强免疫功能。  相似文献   

7.
为了观察苏尼特双峰驼自然发酵乳在体内对小鼠腹水型肝癌H22细胞的细胞周期变化的影响,采取在给小鼠接种肿瘤前4天给予苏尼特双峰驼自然发酵乳,接种肿瘤后继续按预定剂量给予12d的方法进行试验,然后计算抑瘤率、脾脏指数、胸腺指数,检测增殖细胞核抗原(PCNA)表达率,用流式细胞仪检测肿瘤细胞周期变化.结果表明苏尼特双峰驼自然发酵乳高剂量组的抑瘤率为35.83%,中剂量组为31.94%,低剂量组为15.56%;脾脏指数、胸腺指数都显著提高(P<0.01或P<0.05);苏尼特双峰驼天然发酵乳对小鼠H22肝癌肿瘤组织的PCNA表达有下调作用,可阻止肿瘤细胞的增殖,抑制其生长.流式细胞仪检测发现,苏尼特双峰驼自然发酵乳可使肿瘤细胞的细胞周期阻滞在S期.因此说明,苏尼特双峰驼自然发酵乳在体内有抑瘤作用,其机制与提高机体免疫力,使细胞周期阻滞并可能继而诱发凋亡有关.  相似文献   

8.
本试验研究了四君子汤发酵液对S180肿瘤模型小鼠的抗肿瘤作用和对免疫功能的影响。采用血清溶血素法检测荷瘤小鼠体液免疫功能;MTT比色法检测淋巴细胞的增殖和流式细胞术检测T细胞亚群。结果表明,四君子汤发酵液具有明显的抑制肿瘤生长的作用,并且可以增强环磷酰胺（CTX）的抗肿瘤作用,降低CTX免疫抑制的副作用。四君子汤发酵液可以提高免疫器官指数;对肿瘤小鼠脾淋巴细胞转化、血清抗体产生都有促进作用;可以明显提高模型小鼠CD3^＋和CD8^＋T淋巴细胞水平,降低由于肿瘤引起的CD4^＋/CD8^＋T淋巴细胞比值的升高,与模型组比较差异显著（P〈0.05）。研究表明四君子汤发酵液的抗肿瘤作用和增强机体免疫调节功能有关。  相似文献   

9.
在 C57BL/6小鼠的左后肢肌肉注射脂质体包裹的含新城疫病毒 HN基因和鸡贫血病病毒 VP3基因的重组质粒。 2周后 ,在左后肢相同部位皮下接种 B16黑色素瘤细胞 2× 10 5个 ,致瘤 2、10 d后 ,分别肌注相同的重组质粒 .通过细胞毒 T淋巴细胞和自然杀伤细胞 (NK)以及小鼠血清中唾液酸含量的测定 ,探讨新城疫病毒抑瘤的机制以及新城疫病毒 HN基因与鸡贫血病病毒 VP3基因的协同抑瘤效应。结果表明 ,与对照组相比 ,注射含 HN基因和 VP3基因的重组质粒可使荷瘤鼠的肿瘤体积缩小。用新城疫病毒或含新城疫病毒 HN基因重组质粒治疗的荷瘤鼠 ,NK活性、CTL 活性明显高于对照组 (P <0 .0 5 ) ,血清中唾液酸含量低于对照组 ,但差异均不显著 (P >0 .0 5 )。从而证明新城疫病毒 HN基因能够增强小鼠对移植瘤的免疫杀伤力 ,是新城疫病毒发挥抗肿瘤作用的主要功能性基因。  相似文献   

10.
研究E0-E2基因和表达蛋白及抗体水平在小鼠体内的最长存留时间,以确定表达猪瘟病毒E0-E2基因重组腺病毒疫苗(rAd-E0-E2)在小鼠体内的存留规律。本试验选用30只SPF级别的健康小鼠,随机分为A、B组,各免疫组以1×106 IFU/只剂量接种rAd-E0-E2,对照组注射生理盐水。A组20只于接种后不同特定时间(2只/次)采集血液和组织进行PCR和IFA检测E0-E2基因及表达蛋白残留情况;B组10只于接种后1、3、5、7、10、14、21、28d采集血清检测猪瘟抗体水平。PCR和IFA检测显示,在接种12、24、36h后肝、脾、肾组织和血清中E0-E2基因和蛋白检出率为100%,接种48h后小鼠肝、脾组织检出率为50%,肾组织和血清检出率为100%。接种72h以后,小鼠血液和组织检测均为阴性。接种后7d试验组体内抗体阳性率为40%,接种后10d小鼠体内抗体阳性率为100%,抗体阻断率在43%～51.2%,接种后28d,猪瘟抗体阳性率为100%,抗体阻断率在75.6%～87.5%。表明rAd-E0-E2在小鼠体内的存留时间最长72h,接种时间与E2抗体水平呈正相关。  相似文献   

11.
Primary abdominal visceral soft tissue sarcomas (STSs) are rare tumours in dogs with little information available on outcomes. The goal of this retrospective, multi‐institutional study was to describe the common tumour types, location and prognostic factors associated with primary abdominal visceral STSs. Medical records were searched for dogs with primary abdominal visceral STSs at six institutions and were retrospectively reviewed. Tumours were graded using the previously described grading scheme for STSs of the skin and subcutis when information in the histopathology report contained adequate details. Forty‐two dogs were included in the study. Five dogs had grade I tumours, 11 had grade II and 15 had grade III tumours. The most common tumour type was leiomyosarcoma (38.1%). The most common tumour locations were the spleen (47.6%) and small intestine (23.8%). The local recurrence rate was low (4.7%). Metastasis was present at the time of surgery in 23.8%, and the overall metastatic rate was 40.4%. Mitotic index of ≥9 was associated with significantly shorter survival time (MST 269 days) compared with a mitotic index of <9 (MST not reached). The MST for grade I STSs was not reached, was 589 days for grade II and 158 days for grade III. Dogs with grade III tumours were more likely to develop metastatic disease. Neither location of the primary tumour nor the histologic subtype was associated with survival time. Histologic grading of abdominal visceral STSs using the previously described scheme is prognostic and should be provided on histopathology reports.  相似文献   

12.
A 10-year-old Thoroughbred mare was referred to Tennessee Equine Hospital for evaluation and treatment of a subepiglottic mass. Physical and laboratory examinations revealed no other abnormalities. The tumour was excised through a laryngotomy site using transendoscopic laser and sharp excision. Histological evaluation revealed a poorly differentiated sarcoma with features of a leiomyosarcoma, a rarely identified neoplasm in the oropharynx of the horse. The mare developed intermittent dorsal displacement of the soft palate (iDDSP) after surgery but was able to resume full training. There was no evidence of regrowth 8 months post-operatively.  相似文献   

13.
Computed tomographic angiography (CTA) and magnetic resonance imaging (MRI) have been described as methods for preoperative surgical planning in cats with feline injection site sarcomas (FISS), however, few published studies have compared these modalities. The objective of this retrospective, secondary analysis study was to determine if imaging features of FISS on CTA and MRI are predictive of neoplastic peritumoral projections. Archived data from a previous prospective study were retrieved for 10 cats with FISS. All cats had been evaluated in a single anesthetic episode with MRI and dual phase CT (CTA) imaging followed by surgical removal. Histopathological grading and targeted histopathology of imaging‐identified peritumoral projections were performed. Two observers evaluated the CTA and MRI studies for FISS shape, margination, size, enhancement pattern, postcontrast uniformity, pre‐ and postcontrast margination, the number of muscles involved, mass mineralization, and bone lysis. Metal was present in the imaging field of three of 10 cats, resulting in one nondiagnostic MRI. Peritumoral projections were detected in all cats with both imaging modalities, and most were benign. At least one neoplastic peritumoral projection was detected in six cats using MRI, five cats using CTA, and three cats with both modalities. Higher grade FISS were larger than low grade using MRI, and FISS were larger using MRI. Other FISS imaging features using MRI and CTA were similar. Findings supported use of either MRI or CTA for detecting neoplastic peritumoral projections in cats with FISS. Authors recommend CTA for cats with known metallic objects in the scan field.  相似文献   

14.
Canine histiocytic sarcoma (HS) is an aggressive neoplasia with variable clinical course and fatal outcome. The goals of this study were to evaluate a large cohort of canine patients with immunohistochemically confirmed HS and identify clinical prognostic factors. Biopsy submissions to the Michigan State University with tentative HS diagnoses were histologically and immunohistochemically confirmed, medical records collected, and interviews with relevant veterinary clinics conducted. Of 1391 histopathology submissions with a diagnosis containing the word ‘histiocytic’, 335 were suspicious for malignancy, and 180 were consistent with HS and had adequate clinical information recorded. The most commonly represented breeds were Bernese mountain dogs (n = 53), labrador retrievers (n = 26) and golden retrievers (n = 17). Median survival for all dogs in the study was 170 days, and subgroup analysis identified palliative treatment, disseminated HS, and concurrent use of corticosteroids as statistically significant negative factors for survival, in both uni‐ and multi‐variate methodologies.  相似文献   

15.
Ocular sarcoma was diagnosed by light microscopic examination in enucleated globes ( n  = 4), orbital tissue biopsy ( n  = 1) and ocular evisceration contents ( n  = 1) from six cats. To determine if feline leukemia virus (FeLV) or a replication-defective FeLV, feline sarcoma virus (FeSV), was present in these ocular sarcomas, immunohistochemistry (IHC) and polymerase chain reaction (PCR) for FeLV were utilized. Immunohistochemical staining for FeLV glycoprotein 70 (gp70) was performed on all six formalin-fixed, paraffin-embedded tumors using an avidin–biotin complex technique. DNA was extracted from each specimen and a 166 bp region of the FeLV long-terminal repeat (LTR) was amplified by PCR. All tumors were composed primarily of spindle cells; two neoplasms had PAS-positive basement membrane enveloping areas of spindle cells. All tumors involved the uvea and five of six tumors showed transcleral extension, one of which invaded the optic nerve. Immunohistochemical staining for FeLV gp 70 was negative. PCR to amplify a portion of the FeLV LTR was negative. Based on these findings of these limited number of cases, FeLV/FeSV may not play a role in the tumorigenesis of feline ocular sarcomas. However, additional tumors representing all morphological subtypes should be investigated for the presence of viral antigen and DNA. It is important to determine the etiology and pathogenesis of these malignant ocular sarcomas. If the cell of origin and pathogenesis involve ocular and lenticular injury, and FeLV/FeSV is not present, then the clinical management of cases of feline ocular trauma, uveitis and glaucoma may prevent the development of this tumor.  相似文献   

16.
Thirty‐seven cases of histiocytic‐like sarcomas (HLSs) in flat‐coated retriever dogs were evaluated retrospectively. This tumour accounted for 36% of the malignant tumours seen in this breed during the study period. The median age at presentation was 8.2 years. Thirty‐four dogs presented with a swelling or mass in a muscle group or surrounding a joint. The remaining three presented for rib (1), cutaneous (1) or primary splenic origin (1). A high rate of metastasis to local lymph nodes (45%), thorax (20%) and abdominal organs (20% confirmed) was seen. Overall metastastic rate by the time of death was 70%. The median survival for all dogs was 123 days. The most significant prognostic indicator was presence of distant metastasis at the time of diagnosis with median survival of 68 or 200 days, with or without metastasis, respectively. Chemotherapy and radiation therapy significantly improved survival. Dogs given chemotherapy survived a median of 185 versus 34 days for dogs that were not (P = 0.0008). Dogs treated with radiation survived a median of 182 versus 60 days for those that were not (P = 0.0282). Dogs receiving only palliative therapy survived a median of 17 versus 167 days in dogs receiving any kind of radiation, chemotherapy, surgery or combinations. A set protocol of radiation and CCNU (RTCCNU) induced minimal toxicity and provided a median survival of 208 versus 68 days for all other dogs. While this tumour carries a poor long‐term prognosis in flat‐coated retrievers, it is reasonable to treat these dogs for palliation of signs and extension of life.  相似文献   

17.
Histiocytic sarcoma was investigated histopathologically and immunohistochemically in 17 four-toed hedgehogs (Atelerix albiventris), along with a review of their clinical data. Cases were histopathologically classified into two types: round-polygonal cell type (6 cases) and spindle cell type (11 cases). Round-polygonal cell type was found in visceral organs such as the spleen, lymph nodes, and more, and most cases of this type were consistent with disseminated histiocytic sarcoma. On the other hand, spindle cell type occurred mainly in skin, and almost all cases were consistent with localized histiocytic sarcoma. The prognosis of patients with round-polygonal cell type appeared worse than that of spindle cell type. Immunohistochemically, neoplastic cells of spindle cell type showed stronger reactivity against human leukocyte antigen-DR than round-polygonal cell type. Neoplastic cells of all cases showed strong reactivity against ionized calcium-binding adapter molecule-1 (Iba-1) and various reactivities against cluster of differentiation (CD) 204. Regardless of morphological classification, most tumor cells were negative for CD163, suggesting that this marker is less effective for the diagnosis of histiocytic sarcoma. The results of this study suggest that Iba-1 is the most effective marker for histiocytic sarcoma.  相似文献   

18.
The present report describes a rare case of spontaneous primary histiocytic sarcoma of the popliteal lymph node in a 19-week-old female Sprague-Dawley (SD) rat. At necropsy, a 10 mm-diameter whitish nodule was found at the site of the femoral muscle in the right hindlimb. Histopathologically, the nodule comprised large pleomorphic histiocyte-like cells with abundant eosinophilic or foamy cytoplasm. Multinucleated giant cells, necrotic foci surrounded by palisading arrays of epithelioid histiocyte-like cells and phagocytosis of cell debris or erythrocytes by the neoplastic cells were occasionally observed. Invasion of the tumor cells into the surrounding adipose tissue was found focally, but there were no distal metastases. Immunohistochemically, the neoplastic cells were positive for vimentin, CD68 (ED1) and lysozyme. We concluded that this tumor occurred in the popliteal lymph node, considering the anatomical location of the lesion and the presence of the remnants of lymphoid tissue involved in the tumor.  相似文献   

19.
闫大任  王杰  刘双 《野生动物》2010,31(5):252-253
2007年5月,哈尔滨市某北极狐养殖场一患病2岁雄性北极狐,因右后肢肿胀而就诊。经体格检查和X线拍片检查初步确诊为右后肢股骨肿瘤,患病北极狐就诊第2 d死亡,经病理剖检和病理组织学检查分析,诊断为右后肢股骨Ewing肉瘤。  相似文献   

20.
A 16-year-old Thoroughbred mare presented with a 1-year history of a growing mass on the rostral mandible. Biopsy performed by the referring veterinarian revealed chronic granulating fibrosis and stomatitis. Due to progressive loss of body condition and increasing size, the mare presented to the clinic. Radiographic examination of the mandible revealed osteolytic lesions. Mandibulectomy was performed under general anaesthesia. The horse's demeanour and feed intake improved significantly and the cosmetic outcome was acceptable. Histopathological examination confirmed a sarcoma, most likely a fibrosarcoma.  相似文献   

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