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1.
4种单胞藻对卤虫生长和生殖影响的比较研究   总被引:1,自引:0,他引:1  
以亚心形扁藻、小球藻、湛江等鞭金藻、绿色巴夫藻为饵料,卤虫为投喂对象,建立五个实验组分别进行研究,观察不同饵料对卤虫生长和生殖的影响,结果表明:投喂亚心形扁藻的卤虫生长最快,成活率高,生殖量也最多,其次为绿色巴夫藻组,湛江等鞭金藻的饵料效果最差.混合4种单胞藻投喂的效果也较好.  相似文献   

2.
卤虫在WSSV病毒病传播中的媒介作用   总被引:3,自引:0,他引:3  
2003年8月对卤虫进行投喂(浸泡)感染,并进行中国明对虾投喂实验。各组对虾阳性感染率的单因素方差分析比较结果表明,卤虫可以携带有活性的WSSV病毒粒子,并可通过摄食导致对虾间接地携带病毒,但病毒感染能力有限。卤虫病理切片观察结果显示,攻毒后卤虫细胞核没有显著深染及核肿大现象,但无论是横切还是纵切均显示有上皮脱落、部分组织结构松散、细胞结构不完整现象。本试验确定卤虫成体可以携带WSSV并通过投喂感染对虾,使其潜在携带病毒,仔虾阶段投喂成体卤虫应经过严格检疫,制作含有经过检疫卤虫的微囊饵料作为卤虫及其他鲜活饵料的替代品或许是有效的防病措施。  相似文献   

3.
Abstract

Two commercial liquid diet supplements were evaluated as a partial replacement for live foods fed to larval and postlarval Pacific white shrimp, Litopenaeus vannamei. Larvae were stocked in 20-L buckets at a density of 113 shrimp/L and fed one of four diets (5 replicate buckets per diet): (1) a control diet consisting of the diatom, Chaetoceros neogracile, and live Artemia; (2) the control diet plus Treflan (a fungicide); (3) LiquaLife liquid larval diet supplements with reduced concentrations of C. neogracile and Artemia; and (4) Epifeed liquid larval diet supplements with reduced concentrations of Artemia. Diets were evaluated by comparing shrimp rostro-caudal length and survival to PL-8, survival to osmotic and pH stress, as well as shrimp gut fullness, gut lipid content, and fouling. Rostro-caudal length and survival to PL-8 were not significantly different among the four treatments. However, mean survival to PL-8 was 12-34% greater in the Epifeed treatment than in the other three treatments. Shrimp fed Epifeed exhibited greater survival (P < 0.05) when exposed to 7 ppt water for two hours than shrimp in the control treatments. In contrast, there was no significant difference in survival (P > 0.05) among the four treatments when shrimp were exposed to apH of 3.35 for two hours. Although shrimp fed liquid diet supplements had more fouling (P < 0.05) than shrimp in either of the control treatments, commercial liquid diet supplements can be used in penaeid shrimp hatcheries to partially replace Artemia without compromising shrimp growth or survival.  相似文献   

4.
Newly hatched Crangon zoeae from different maternal shrimp were raised separately using an Imhoff cone larval-rearing system. Experimental diets were Chaetoceros gracile , live Artemia nauplii, lyophilized Arremia nauplii particles, calcium alginate microencapsulated (CAM) Artemia nauplii, lyophilized Daphnia magna and CAM Daphnia magna . A dependable "atomizer" design for lab oratory preparation of CAM diets was developed. Initially, survival rates of zoeae fed either Chaetoceros or live Artemia nauplii were evaluated in order to establish a suitable control diet for subsequent growth experiments, and the interbrood and intrabrood components of zoed survival variation were analyzed.
Crangon zoeae ate every type of food offered to them, but only Chaeroceros- fed zoeae survived to metamorphosis. Zoeal survival rates were extremely variable. Interbrood variation was greater than intrabrood variation, but the difference was not statistically significant. Zoeae from smaller maternal shrimp were generally more active and had greater survival rates than zoeae from larger, presumably older and senescent shrimp. Zoeae that were fed Chaetoceros supplemented with CAM Artemia nauplii had significantly greater ( P < 0.001) growth rates than siblings fed only Chaetoceros .
Suspension feeding on Chaetoceros was probably more efficient than raptorial feeding on Artemia nauplii, resulting in greater survival rates. Supplementing a successful microalgal diet with CAM Artemia nauplii allowed greater use of zoeal feeding mechanisms, added an animal nutrient source to the diet, and affected growth rates by increasing the molt increment and decreasing the intermolt period.  相似文献   

5.
Minced Taiwan mauxia shrimp Acetes intermedius , an alternative to brine shrimp Artemia sp. nauplii, has long been used as a principal or supplemental feed in prawn hatcheries in Southeast Asia and India. In this study, black tiger prawn Penaeus monodon postlarvae were fed with frozen brine shrimp nauplii (diet B) or minced Taiwan mauxia shrimp (diet M) for 4 wk to compare their astaxanthin concentration and composition, survival, and growth. Diet B contained two and a half times more total carotenoid (TC) than diet M, with canthaxanthin as a major (79%) carotenoid, but without astaxanthin, a predominant carotenoid in crustaceans. Of its TC in diet M, 55% was β-carotene and 17% astaxanthin. B-prawn (postlarvae fed with diet B) had a similar growth rate as M-prawn and twice the survival rate as M-prawn. The concentrations of total astaxanthin (TA), free astaxanthin (FA), astaxanthin monoester (MA), and astaxanthin di-ester (DA) in B-prawn were all higher than those in M-prawn. Except for FA, no reduction of TA, MA, or DA concentration during the feeding interval was found in B-prawn. However, concentrations of TA, FA, MA, and DA in M-prawn all decreased. TC content of the experimental diets had a greater influence on resulting prawn astaxanthin concentration and composition than carotenoid compositions of the diets.  相似文献   

6.
波纹龙虾早期叶状幼体的培育及其形态观察   总被引:1,自引:0,他引:1  
为了探索波纹龙虾(Panulirus homarus)幼体培育新方法,并系统描述其幼体形态特征.在室内以5种不同的饵料组合投喂波纹龙虾早期叶状幼体.结果表明,扁藻(Platymonas sp.) 卤虫(Artemia salina)组的存活率和变态率最高,到48 d后,幼体存活率达25%,并25%的幼体能顺利完成6次蜕皮;卤虫组、角毛藻(Chaetoceros sp.) 卤虫组的存活率和变态率较低,没有幼体能顺利完成4次蜕皮.对叶状幼体的形态特征进行观察,发现刚孵出和蜕皮1次的叶状幼体无眼柄,第2次蜕皮后开始出现眼柄,第3次蜕皮后开始出现第4步足,第5次蜕皮后开始出现第5步足,在随后的几次蜕皮后,第4步足明显增长,第9次蜕皮后第4步足出现带有羽状刚毛的外肢.以扁藻 卤虫作为饵料时,波纹龙虾早期叶状幼体培育效果较好,并获得了脱皮9次的叶状幼体.  相似文献   

7.
Abstract. A feeding experiment was conducted in aquaria to evaluate growth, survival and food consumption by paddlefish, Polyodon spathula (Walbaum), fry fed live Daphnia pulex, brine shrimp nauplii, Artemia sp. (L.), from two different geographical sources, or one of three commercial dry diets. Fry were fed from first feeding (day 8 post-hatch) to day 17 post-hatch. All diets had similar percentages of crude protein and lipid. Fatty acid composition was similar in all diets except for Great Salt Lake brine shrimp nauplii which had a higher percentage of linolenic acid, 18:3 (n-3), and a lower percentage of eicosapentaenoic acid, 20:5 (n-3). Paddlefish fed live food organisms were significantly (P<0·05) larger than those fed non-living diets. Survival was significantly higher (P<0·05) for fish consuming live Daphnia (95·3%) than all other treatments. Percentage of fish with food in the digestive tract did not significantly differ (P>0·05) among treatments. Poorer growth and survival by fish fed prepared diets may have been due to limited digestibility of the dry diets by paddlefish.  相似文献   

8.
几种植物浆养殖卤虫的饵料效果   总被引:11,自引:0,他引:11  
黄旭雄 《水产学报》2000,24(3):254-258
以各种植物浆为饵料培养卤虫,培养效果差异显著,陆生植物优于水生植物.其中以黄豆叶浆养殖的卤虫成活和生长最佳,第16天时平均存活率(69.6%)和体长(6.436±0.956mm)与螺旋藻粉组相似(分别为66.0%、6.461±1.181mm)而优于酵母组(分别为29.8%、5.216±1.030mm).以黄豆叶浆养殖的卤虫的粗蛋白及氨基酸含量略低于酵母和螺旋藻粉喂养的卤虫,在高密度卤虫养殖中可替代部分螺旋藻粉,降低卤虫养殖的饵料成本.  相似文献   

9.
Larvae of the freshwater prawn, Macrobrachium rosenbergii (De Man), and the striped bass, Morone saxatilis (Walbaum), were fed artificial diets manufactured by a spray-drying process or live Artemia nauplii , separately or in various combinations. Spray-dried diets were neutrally buoyant, water stable (low protein leaching rate), and were satisfactorily consumed as verified by the observation of full guts after feeding. Survival and growth of larvae fed live Artemia nauplii was significantly greater than those of larvae fed spray-dried diets. Lowest survival and growth rates were observed when larvae were fed artificial diets exclusively from the onset of exogenous feeding. Survival rates were positively related to the duration of feeding live Artemia nauplii before weaning to artificial diets. The poor performance associated with spray-dried artificial diets may be the result of the digestive capabilities of the larvae of caridean shrimp and striped bass being insufficient to process compact, dense particles.  相似文献   

10.
The use of dried Artemia biomass meal as an exclusive feed for postlarval white shrimp (Litopenaeus vannamei) was compared with four commercial feeds and three crustacean meals in a series of trials. Postlarvae (PL1–PL6) were stocked at a density of 1.5–2.5/litres in 16 tanks (100 litres volume) and fed, ad libidum, five times a day, over 23–29 days. Feeding postlarval shrimp with dried Artemia biomass resulted in a significantly larger size than feeding with three of the commercial feeds, and the crustacean meals. There was no significant size difference observed in animals fed with Artemia biomass and the commercial 'Golden Pearls' feed for postlarvae, however the coefficient of variation among the size of the 'Golden Pearls' fed animals was significantly higher. The weight increase of animals fed with Artemia biomass was higher than in animals fed with all the tested feeds. The survival rate was not significantly different in animals fed with Artemia flakes from 'Salt Creek', 'Bio-Marine', 'Golden Pearls' and Artemia biomass, however the survival rate was significantly larger in animals fed with Artemia biomass than in animals fed with the crustacean meals and 'Artemac.' Results suggest that dried Artemia biomass is a well-suited feed for postlarval L. vannamei.  相似文献   

11.
Penueus vannumei larvae (P2 through M3) and early postlarvae (<24 h old postlarva) were fed diets consisting of algae-only, nematodes ( Panugrellus redivivus ) plus algae or Artemia plus algae. Growth (dry biomass gain) of second and third stage protozoea larvae fed the nematodealgae diet was significantly better than that of larvae fed the Artemia-algae diet. From the first mysis through the postlarval substage (<24 h old), growth of shrimp fed the nematodealgae diet equalled that of larvae fed the Artemia-algae diet. All larval substages fed nematodes plus algae accumulated significantly greater biomass than those fed a diet of only algae. Survival and percent metamorphosis of larvae fed nematodes plus algae did not differ significantly from that of larvae fed either Artemia plus algae or algae alone. A nematodealgae feeding regime, which potentially yields growth, survival and metamorphosis equal to that obtained on the standard Artemia plus algae regime, is proposed for P. vannamei .  相似文献   

12.
Nauplii of the brine shrimp, Artemia , and cultures of the soil nematode, Panagrellus redivivus , were fed a suspension of the water-insoluble antibacterial Romet-30 (sulfadimethoxine plus ormetoprim) to determine if they could be used as a mechanism for delivery of the drug to larval penaeid shrimp.
Romet-30 was added to groups of brine shrimp nauplii and nematodes in 1 L beakers to achieve a concentration of 3 mg of drug per ml of seawater. After a 4 h uptake period, predetermined numbers of organisms were assayed for antibiotic content using the micro-plate diffusion method and a Romet-30 sensitive strain of Vibrio alginolyticus as the indicator. The resulting zones of inhibition were compared to that of a standard Kirby-Bauer sensitivity disc containing 25 μg of the drug that was placed on each plate as standard control. Negative controls consisted of equal numbers of nauplii and nematodes that were not exposed to the antibiotic. Artemia nauplii and nematodes accumulated approximately 0.1 μg of the drug per nauplius and approximately 0.25 μg per nematode. They exhibited no signs of toxicity from exposure to the drug and contained the antibiotic in sufficient quantity to provide a theoretical therapeutic dose of the drug when fed to penaeid larvae.
In subsequent tests, normal and diseased larval Penaeus stylirostris readily accepted Romet-30 enriched Artemia . Diseased larvae fed the drug in this manner exhibited a statistically greater overall survival and a greater survival to the post-larval stage by Student's t -test at the 5% significance level.  相似文献   

13.
The use of dried Artemia biomass meal as an exclusive feed for postlarval white shrimp (Litopenaeus vannamei) was compared with four commercial feeds and three crustacean meals in a series of trials. Postlarvae (PL1–PL6) were stocked at a density of 1.5–2.5/litres in 16 tanks (100 litres volume) and fed, ad libidum, five times a day, over 23–29 days. Feeding postlarval shrimp with dried Artemia biomass resulted in a significantly larger size than feeding with three of the commercial feeds, and the crustacean meals. There was no significant size difference observed in animals fed with Artemia biomass and the commercial 'Golden Pearls' feed for postlarvae, however the coefficient of variation among the size of the 'Golden Pearls' fed animals was significantly higher. The weight increase of animals fed with Artemia biomass was higher than in animals fed with all the tested feeds. The survival rate was not significantly different in animals fed with Artemia flakes from 'Salt Creek', 'Bio-Marine', 'Golden Pearls' and Artemia biomass, however the survival rate was significantly larger in animals fed with Artemia biomass than in animals fed with the crustacean meals and 'Artemac.' Results suggest that dried Artemia biomass is a well-suited feed for postlarval L. vannamei.  相似文献   

14.
A 20‐week feeding trial was conducted to measure growth, nutrient utilization and faecal/gut bacterial counts in triplicate groups of red hybrid tilapia, Oreochromis sp., when fed diets supplemented with 0.5% organic acids blend (OAB), 1.0% OAB, 0.5% oxytetracycline (OTC) or a control diet (no additives). At the end of the feeding trial, tilapia were challenged with Streptococcus agalactiae for 22 days. Fish fed the OTC diet had significantly higher (P < 0.05) growth than the control treatment, while growth between fish fed the OTC or OAB diets was not significantly different (P > 0.05). Phosphorus, dry matter and ash digestibility were significantly higher in the 1.0% OAB diet than the control diet. Fish fed the OAB diets had significantly lower colony‐forming units of adherent gut bacteria compared to the control or OTC treatments while those fed the 1.0% OAB diet had the lowest total faecal bacterial counts. Tilapia fed the 0.5% OTC or OAB diet had significantly higher resistance to S. agalactiae than those fed the control diet. This study indicates that dietary organic acids can potentially replace OTC as a growth promoter and antimicrobial in tilapia feeds.  相似文献   

15.
Oxytetracycline (OTC) in shrimp shells may be dispersed to the environment as shrimp shred old cuticle in growout ponds. The study aims to assess the kinetics of OTC accumulated in shrimp shell. Sub‐adult male Litopenaeus vannamei in the C–D0 molting stage, were force fed with medicated feeds at various accurate dose levels that included 50, 500 and 1000 mg/kg‐body weight (BW). In addition to hemolymph, hepatopancreas and muscle were serially collected for 50 mg/kg‐BW‐dose group while cuticle was sampled for higher dose levels. All were assayed for OTC by a validated high‐performance liquid chromatography (HPLC) method. Mineral contents in shell samples of 500 mg/kg‐BW‐dose were also determined. The bioavailability markedly decreased with increasing dose due to incomplete dissolution and/or mild dysfunction in absorption. Administered doses, 2.69 and 2.25%, ended up in the shell after dosing with 500 and 1000 mg/kg‐BW, respectively. OTC data after a dose of 50 mg/kg‐BW was fitted into a three‐compartment model with an added shell compartment with r2 of 0.9920. The model was successfully extrapolated to predict OTC distribution in shell at higher doses. In addition, there was evidence that OTC may disturb the biomineralization process via complex formation with calcium and magnesium lowering the exoskeleton mineral contents.  相似文献   

16.
Abstract— The effects of four kinds of foods ( Artemia nauplii, rotifer, and microalgae Chaetoceros gracilis and Isochrysis galbana ) and four temperature regimes on survival and development of larval Lysmata wurdemanni , a marine ornamental shrimp, were determined. The larvae fed with Chaetoceros or Isochrysis only survived for a maximum of 17 d, before developing to zoea IV. The survivorship of the larvae fed with Artemia nauplii or rotifer from zoea II to post-larvae was 66.7% and 68.9%. respectively, without significant difference ( P > 0.05). But larvae fed with Artemia nauplii grew significantly ( P < 0.05) faster than those fed with rotifer. Larvae fed with Artemia nauplii reached postlarvae in 29–32 d, compared with 32–36 d in the rotifer treatment. Artemia nauplii are also suitable food for the postlarvae and juveniles of L. wurdemanni . The 30 postlarvae fed on Artemia nauplii all survived to reach sexual maturity in 50 to 70 d, growing from about 7 to 28 mm in total length. The effects of four temperature regimes on larval development of L. wurdemanni was also investigated. The duration (mean ± SD d) to the postlarvae at temperatures of 26 C (37.4 ± 5.4) and of 26–30 C daily (40.2 ± 5.8) was significantly ( P < 0.05) longer than that at 28.5 C (29.3 ± 4.8) and at 27–29 C daily (28.7 ± 3.5).  相似文献   

17.
饵料对不同品系卤虫生长和生殖的影响   总被引:2,自引:0,他引:2  
用盐藻、米糠、螺旋藻粉、酵母、米糠 盐藻这5种饵料类型培养江苏台北盐场、西藏巴里坤湖、山西运城、美国大盐湖等4个品系的卤虫,考察其生长、存活和繁殖特性.结果表明米糠、螺旋藻、米糠 盐藻利于卤虫的生长,盐藻和米糠 盐藻利于卤虫的成活,米糠、螺旋藻、米糠 盐藻可促进卤虫的繁殖;美国大盐湖品系的综合性能最佳,以它作为增养殖对象是较好的选择。  相似文献   

18.
饵料和养殖密度对中国对虾幼虾生长及存活率的影响   总被引:6,自引:0,他引:6  
在实验用虾遗传背景一致的情况下,分析了3种饵料(配合饲料、冰冻鲜鱼肉和活卤虫)和4个不同的养殖密度对小水体中国对虾幼虾生长和存活率的影响。结果表明,饵料和饲养密度对中国对虾幼虾生长及存活率有显著影响。在饵料、养殖密度单因子实验及饵料和养殖密度相结合的双因子实验中,幼虾的生长均表现出极其显著的差异(P<0.01),活卤虫对幼虾生长的效应尤为突出。而养殖密度对中国对虾的行为生物学、个体间体重增量均有影响。随着养殖密度的提高,中国对虾增重变慢;同时,个体间体重增量差异变大。随着养殖密度的增加,中国对虾幼虾的存活率呈下降趋势,但不同饵料对存活率影响变化幅度较大,波动在58.1%~85.2%之间,其中投喂活卤虫养殖密度为50尾/桶的存活率最高(85.2%);投喂配合饵料4个养殖密度梯度的存活率变化不明显;而投喂冰冻鱼肉4个养殖密度梯度的存活率变化较大。因此,采用合适的饵料和养殖密度,能够提高中国对虾的生长速度,提高对虾养成存活率,改善生长过程中对虾群体体长、体重的整齐度。  相似文献   

19.
Experimental studies were conducted by injecting or feeding white spot syndrome virus (WSSV) derived from infected shrimp, Penaeus monodon (Fabricius), collected from the south-east coast of India, to five species of shrimp, two species of freshwater prawns, four species of crabs and three species of lobsters. All species examined were susceptible to the virus. Experimental infections in the shrimp had the same clinical symptoms and histopathological characteristics as in naturally infected P. monodon . A cumulative mortality of 100% was observed within 5–7 days in shrimp injected with WSSV and 7–9 days in shrimp fed with infected tissue. Two species of mud crab, Scylla sp., survived the infection for 30 days without any clinical symptoms. All three species of lobsters, Panulirus sp., and the freshwater prawn, Macrobrachium rosenbergii (De Man), survived the infection for 70 days without clinical symptoms. However, bioassay and histology using healthy P. monodon revealed that crabs, prawns and lobsters may act as asymptomatic carriers/reservoir hosts of WSSV. This is the first report to suggest the carrier/reservoir capacity of these hosts through histological and bioassay evidences. Ultrastructural details of the virus in experimentally infected shrimp, P. vannamei , (Boone), were also studied.  相似文献   

20.
Recent improvements in enriching Artemia make mahimahi culture possible without using copepods. Mahimahi survive better when cultured copepods Euterpina acutifrons are used, especially when the larvae are under stresses such as high stocking density, cold weather, or the rigors of metamorphosis. This study looked at some differences between copepods and enriched Artemia .This study tested stress resistance by holding postlarval (PL) mahimahi out of water in a hand net for varying periods of time. Recovery from this stress shock was higher in PLs whose diet was higher in docosahexaenoic acid (DHA). DHA appeared to play an important role in stress resistance. High eicosapentaenoate (EPA) or high HUFA did not confer stress resistance when DHA levels were low. Copepods contained higher levels of many essential amino acids in addition to higher levels of DHA compared with enriched brine shrimp.
Larvae sickened by disease appeared to lose fat, including DHA, but they conserved DHA relative to other fatty acids. Even when sick, mahimahi larvae fed a copepod diet resisted stress better than larvae fed an enriched brine shrimp diet.
Optimal Artemia enrichment levels of DHA for mahimahi have not been reached and this may explain why it has been difficult for others to raise this fish consistently.
E. acutifrons is relatively easy to culture, but may not be practical for commercial hatcheries, due to low yield (less than 10 g per 100 L per week).  相似文献   

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