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1.
In 159 comparative studies with fully grown sheep and heifers of the digestibility and of rumen physiologic values at a variation of the feed ration in the crude fibre content between 112 and 318 g, in the crude protein content between 94 and 194 g and in the content of nitrogen free extractive between 484 and 641 g/kg DM is provided that the digestibility of energy in heifers was, on average, 4 digestibility units lower than in sheep. The corresponding values for the digestibility of crude protein and crude fibre are 12 and 3.5 digestibility units. Due to the lower methane energy excretion of the heifers the difference at the level of metabolizable energy is reduced to 1.5% of the gross energy. The level of the differences in digestibility is influenced by the development of the young cattle and the composition of the rations. In young cattle of the live weight range between 100 and 150 kg the digestibility of the energy is 6-8 units lower than in sheep. A higher crude fibre content in the ration diminishes the difference in the digestibility of energy and the nutrients between the two animal species.  相似文献   

2.
This study examined intraspecific variation in mineral composition and rumen degradability of Atriplex nummularia plants and the influence on selection preferences of sheep. Individual plants were categorised into high, medium and least preference groups by assessing the order in which they were selected by sheep. Nine plants were selected from each group and the regrowth of these plants was analysed for neutral detergent fibre (NDF), crude protein (CP), mineral composition and rumen degradability of dry matter. The data was subjected to one-way and multivariate analyses of variance. Highly preferred plants had a higher concentration of CP, phosphorous (P) and magnesium (Mg) in their edible forage compared to the medium or least preferred plants. Individual preferences of sheep were not, however, associated with the rumen degradability parameters. Principal component analysis revealed that highly preferred plants had lower NDF, manganese (Mn) and zinc (Zn), and higher CP, calcium, P, Mg and potassium values compared to the least preferred plants. In contrast, medium preferred plants exhibited inconsistent patterns, with a tendency to have higher sodium chloride and sodium, and lower Mn, Zn and copper, concentrations in the forage.  相似文献   

3.
The experiment aimed to study the difference on the dietary nutrient digestibility of different genetic basis beef cattle during two nutrient levels.Forty-eight heads of different genetic basis beef cattle （Charolais×Simmental-crossbred cattle,Gelbvieh×Simmental-crossbred cattle,Angus×Simmental-crossbred cattle and Limousin×Simmental-crossbred cattle) were introduced，feeding two different nutritional level of diets，adopting 4×2 two factor design，and carrying on the seven days of digestion experiment using endogeny indicator method.The results showed as follows：①From genetic basis，crude protein digestibility in Gelbvieh×Simmental-crossbred cattle was significantly higher than that in Angus×Simmental-crossbred cattle (P＜0.05)，and extremely significantly higher than that in Limousin×Simmental-crossbred cattle（P<0.01）, crude fat digestibility in Gelbvieh×Simmental-crossbred cattle was extremely significantly higher than that in Charolais×Simmental-crossbred cattle and Angus×Simmental-crossbred cattle (P＜0.01).②From nutritional level，the nutrient digestibility of beef cattle in feeding a high nutrition diet were extremely significantly higher than that in feeding a low nutrition diet (P＜0.01).③From the interaction of genetic basis and nutritional level，nutrient digestibility decreased with the increase of nutritional level of diet in Charolais×Simmental-crossbred cattle,Angus×Simmental-crossbred cattle and Limousin×Simmental-crossbred cattle，while Gelbvieh×Simmental-crossbred cattle were on the contrary.The results indicated that genetic basis had significant influences on crude protein digestibility and crude fat digestibility （P＜0.05），nutritional level of diet had significant influences on the digestibility of dry matter，organic matter，crude protein，crude fiber，crude ash and crude fat （P＜0.05），crude protein digestibility were influenced significantly by genetic basis and nutritional level of diet at the same time （P＜0.05）.  相似文献   

4.
Despite indications of a considerable spread of Maedi-Visna among sheep flocks in Germany, prevalence studies of this important infection are hardly available. Prior to any health schemes and guidelines, knowledge about regional disease distribution is essential. Depending upon herd size, 70 farms were randomly selected, of which 41 cooperated. A total of 2229 blood samples were taken at random and serologically examined. For assessment of selected farm characteristics a questionnaire exercise was conducted at all farms involved. The average herd prevalence is 51.2%, the within-herd prevalence is 28,8%. In the unvariate analysis of risk factors, small (10-100 sheep) and large (> 250 sheep) farms are more MVV-affected than medium sized farms. The average stable and pasture space per sheep is larger at non-infected- compared to infected farms. Owners judgement on general herd health turns out to be better at non-infected compared to infected farms. Taking infected farms only, the risk of within-herd prevalence above 20% is significant higher in crossbred than in purebred flocks.  相似文献   

5.
Rations with energy parts of 50, 25 and 10% from barley, maize and potatoes were investigated comparatively by means of total metabolism experiments with oxen with a view to arriving at a more precise estimation equation of net energy fat for cattle. Parallel to the investigations with oxen the energy and nutrients digestibility of the rations in wethers was measured. The crude fibre content of the rations ranged from 166 to 271 g and the starch content from 69 to 330 g/kg DM. The daily starch intake of the oxen ranged from 575 to 2739 g on nutrition level (NL) 1.7 and from 365 to 1804 g on NL 1.1. The energy digestibility of the rations in oxen with energy parts of 50% barley, maize and potatoes was on average 73.5, 73.9 and 75.3%, of the rations with energy parts of 25% on average 72.2, 71.6 and 72.4% and of the rations with energy parts of 10% on average 68.8, 69.5 and 69.8%. The digestibility of energy and nutrients in cattle and sheep was in good agreement excepted crude protein and crude fat; these were digested 4-5%-units lower from cattle than from sheep. The increase of the nutrition level by one unit lowered the digestibility of rations with energy parts of 50 and 25% from concentrates in cattle about 3-6 units and of rations with energy parts of 10% from concentrates about one %-unit. Information about rumen physiological data is given comparatively between cattle and sheep.  相似文献   

6.
[目的]试验旨在研究不同蛋白源饲料对秦川肉牛日增重、屠宰性能及肉品质的影响。[方法]选择20头体重相近、健康状况良好的12月龄左右的秦川肉牛(母牛)为试验动物,随机将试验牛分为5组,各组分别饲喂5种不同精饲料。试验结束后,对照组(31%豆粕组)、试验Ⅰ组(36%菜籽粕组)和试验Ⅳ组(42%紫苏饼组)各选3头参试牛进行屠宰。[结果]试验Ⅳ组的全期总增重、平均日增重和屠宰率均显著高于对照组(P<0.05);试验Ⅳ组背最长肌中干物质和粗蛋白含量均高于其他两组,但各组间均差异不显著(P>0.05);对照组和试验Ⅰ组大理石花纹均是3级,试验Ⅳ组大理石花纹是4级。[结论]本试验条件下,精饲料中添加42%的紫苏饼能够提高肉牛日增重和屠宰率,能够提高牛肉中氨基酸的含量和粗蛋白水平。  相似文献   

7.
Ten reared cows of a Japanese Black cattle herd in Kagoshima prefecture, Japan, exhibited extremely low blood urea nitrogen (BUN) concentration (2.6 ± 0.6 mg/dL). Examination of dietary feed nutrition and relevant pastureland soil content suggested a correlation with crude protein (CP) deficiency or unbalanced nutritional dietary feeds. Thirteen months after the introduction of a dietary remedial measure (bean cake supplementation), BUN, total cholesterol and albumin concentration from five of the original 10 cows increased significantly compared with their values of before the dietary remedy. The postpartum day open period was significantly lower after the dietary remedial measure than that before it. The abnormally low BUN levels of the cattle herd may be due to inadequate dietary nutritional content, primarily from the imbalance of total digestible nutrient and CP of the feed and far lower han average CP value. In conclusion, routine examination of serum biochemical parameters in Japanese Black breeding cattle may be a useful strategy for determining subclinical metabolic failure of cattle herds, and consequently, its effect on reproductive performance of the herd.  相似文献   

8.
Cyanide detoxification is catalysed by two enzymes: rhodanese [thiosulphate: cyanide sulphurtransferase, E.C. 2.8.1.1], and 3-mercaptopyruvate sulphurtransferase [3-MST, EC. 2.8.1.2]. In the present work, the activity of the two enzymes in the crude extracts of different tissues and in the mitochondrial and cytosolic fractions of tissues from some ruminants (camels, cattle and sheep) and birds (chickens and pigeons) have been compared. Rhodanese activity was almost exclusively present in the mitochondrial fraction. In ruminants and chickens the highest activity of rhodanese was found in the liver, followed by the kidney. In pigeons, however, the enzyme activity was the highest in the kidneys. In camels' tissues, the rhodanese activity was significantly (P < 0.05) lower than in cattle or sheep, and the enzyme activities in the two latter species were similar. The activity of 3-MST in the crude extract of tissues from camels was similar to that in sheep, but higher than that in cattle. The enzyme activity was equally distributed between the mitochondrial and cytosolic fractions in the liver and kidneys of camels, cattle and sheep.  相似文献   

9.
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10.
Fourteen isolates of Bacteroides nodosus were cultured from cattle on six farms and examined for colony morphology, pilation, agglutinability, proteolytic activity and pathogenicity for sheep. Where sheep were also present on the farms, isolates from these were compared with those from cattle.Colonies of the bovine isolates were moderately fimbriate. Cells from these colonies were pilated, but not to the extent observed on virulent sheep isolates. The proteolytic activity of the isolates was also less than that described for virulent ovine isolates. When inoculated into sheep, B. nodosus from cattle produced only mild interdigital inflammation. There was no separation of horn characteristic of virulent foot-rot. Preliminary studies, based on agglutination tests, suggest that B. nodosus with different surface antigens may occur in cattle in the same herd and in cattle and sheep on the same farm. On one of six farms isolates from cattle and sheep were indistinguishable by the agglutination test.  相似文献   

11.
Monoclonal antibody-based competitive ELISA (C-ELISA) has been used for the specific measurement of antibodies to peste des petits ruminants (PPR) viruses in sheep, goats, cattle and Buffalo. Serum samples from sheep (n = 232), goats (n = 428), cattle (n = 43), buffalo (n = 89) were tested. The animals had not been vaccinated against rinderpest or PPR. Findings suggested that the sero-positive cases were significantly higher in sheep (51.29%) than in goats (39.02%) (P = 0.002). The overall sero-prevalence of PPRV in small ruminants was 43.33%. The PPR antibodies seroprevalence was 67.42% in buffalo and 41.86% in cattle which was significantly higher in buffalo (P = 0.005). The overall sero-prevalence of PPRV in large ruminants was 59.09%. Cattle and buffalo sera showed a high prevalence of antibody against PPR virus which may explain the difficulty experienced in achieving high post-vaccination immunity levels against rinderpest. Because antibodies against PPR virus are both cross-neutralizing and cross-protective against rinderpest virus, further vaccination in the presence of antibodies against PPR virus may be a waste of national resources. It was also suggested that antibodies to PPR virus could prevent an immune response to the rinderpest vaccine. This paper presents serological evidence for the transmission of PPR virus from sheep and goats to cattle and buffalo and highlights the need to include PPR serology in the sero-monitoring programme to give a better indication of national herd immunity of sheep and goats against PPR.  相似文献   

12.
A study was conducted in 1994-96 with the aim of assessing the serological prevalence of Border Disease (BD) among sheep and goats in Denmark and to investigate possible relations to herd factors. From each of 1000 herds, 2 blood samples were obtained from animals older than 1 year. The examination for antibodies was performed using a blocking ELISA detecting antibodies to pestivirus. Data from 815 herds were analysed statistically by the maximum likelihood method in a multinomial model. The estimated herd prevalence was 0.083 and the estimated individual prevalence within the positive herds was 0.50. There was no difference between the prevalence in sheep and goat herds. Records for well over half of the herds could be combined with data from the Danish Central Husbandry Register. No association between occurrence of BD and herd size was found. Cattle were registered as contemporarily present on 135 out of 521 herds which was shown to be strongly associated to BD. The estimated herd prevalences of BD among farms with and without contemporary cattle were 0.24 and 0.042, respectively.  相似文献   

13.
SRY是Y染色体上具体决定生物雄性性别的基因片段,是多数哺乳动物性别决定基因之一。本试验采用克隆测序SRY基因并结合生物信息学对其序列进行分析,利用软件Codon W分析麦洼牦牛、普通牛、绵羊、山羊、猪、小鼠、鸡和人该基因编码区的密码子偏好性。克隆测序得到SRY基因序列含1个690 bp的开放阅读框,共编码229个氨基酸;其编码区核苷酸序列与普通牛、绵羊、山羊、猪、小鼠、鸡、人相应序列间的一致性分别为99.9%、93.9%、91.2%、76.5%、43.3%、21.4%、69.1%。经聚类分析,麦洼牦牛首先与普通牛聚在一起,绵羊与山羊聚为一类,这两类相聚后再依次与猪、人、小鼠相聚,最后与鸡聚为一类,其结果与以往的生物学分类结果一致。该基因编码的蛋白质分子式为C1155H1827N351O348S11,相对分子质量为2655.0,理论等电点PI为9.55,亲水性平均数为-0.855。其密码子偏好性分析显示,T3s为0.2905、C3s为0.3240、A3s为0.3728、G3s为0.2963,第3位碱基中出现G和C占第3位碱基总量的48%,同义密码子数为61。上述数据表明麦洼牦牛SRY基因编码序列与普通牛、绵羊、山羊、猪、人具有较高的一致性,在物种进化过程中较为保守。该基因对含A的密码子有较高的偏爱性,尽量避开以G结尾的密码子,且其所编码的蛋白质为亲水性蛋白。  相似文献   

14.
OBJECTIVE: To examine healthy slaughter-age cattle and sheep on-farm for the excretion of Salmonella serovars in faeces and to identify possible risk factors using a questionnaire. PROCEDURE: The study involved 215 herds and flocks in the four eastern states of Australia, 56 with prior history of salmonellosis. Production systems examined included pasture beef cattle, feedlot beef cattle, dairy cattle, prime lambs and mutton sheep and animals were all at slaughter age. From each herd or flock, 25 animals were sampled and the samples pooled for Salmonella culture. All Salmonella isolated were serotyped and any Salmonella Typhimurium isolates were phage typed. Questionnaires on each production system, prepared in Epi Info 6.04, were designed to identify risk factors associated with Salmonella spp excretion, with separate questionnaires designed for each production system. RESULTS: Salmonellae were identified in all production systems and were more commonly isolated from dairies and beef feedlots than other systems. Statistical analysis revealed that dairy cattle were significantly more likely to shed Salmonella in faeces than pasture beef cattle, mutton sheep and prime lambs (P<0.05). A wide diversity of Salmonella serovars, all of which have been isolated from humans in Australia, was identified in both cattle and sheep. Analysis of the questionnaires showed access to new arrivals was a significant risk factor for Salmonella excretion on dairy properties. For beef feedlots, the presence of large numbers of flies in the feedlot pens or around stored manure were significant risk factors for Salmonella excretion. CONCLUSION: Dairy cattle pose the highest risk of all the slaughter-age animals tested. Some of the identified risk factors can be overcome by improved management practices, especially in relation to hygiene.  相似文献   

15.
［目的］研究性别、部位对肉质的影响,了解德宏高峰牛肉质特性。［方法］选择放牧饲养的德宏高峰牛为试验动物,屠宰分割后取肉样,进行失水率、剪切力、蒸煮损失、肉色、水分、粗蛋白、粗脂肪、灰分测定。［结果］阉割公牛枕头肉的L*值显著(P<0.05)高于公牛和母牛,并且母牛L*值显著(P<0.05)高于公牛。阉割公牛辣椒肉L*值显著(P<0.05)高于公牛。母牛辣椒肉失水率显著(P<0.05)低于阉割公牛,阉割公牛眼肉的水分显著(P<0.05)高于公牛和母牛。辣椒肉剪切力极限著(P<0.01)低于眼肉和枕头肉,显著(P<0.05)低于霖肉。枕头肉L*值极限著(P<0.01)高于霖肉,显著(P<0.05)高于眼肉和辣椒肉,b值显著(P<0.05)高于霖肉。眼肉水分极限著(P<0.01)低于枕头肉、霖肉和辣椒肉。眼肉粗蛋白极限著(P<0.01)高于霖肉。［结论］性别对肉色有显著影响,对失水率和水分含量有影响。部位对肉质有显著影响。市售德宏高峰牛牛肉品质有待进一步提高。  相似文献   

16.
Five bluetongue virus (BTV) diagnostic tests were evaluated for use in free-ranging bighorn sheep. We sampled one bighorn sheep population four times between 1989 and 1995. The tests evaluated included virus isolation (VI), polymerase-chain reaction (PCR), serum neutralization (SN), agar-gel immunodiffusion (AGID), and competitive enzyme-linked immunosorbent assay (c-ELISA). The c-ELISA, AGID and SN tests had high levels of agreement in determining serogroup exposure in bighorn sheep. We used maximum-likelihood algorithms to estimate the parameters of each diagnostic test used. Although the c-ELISA and AGID had high sensitivity and specificity, the SN had perfect specificity but lower apparent sensitivity. Due to the potential of cross-reactions among multiple serotypes, results of the SN must be interpreted with caution when assessing serotype exposure in an area where multiple serotypes are endemic. The PCR assay delineated convalescent antibody titers from more-recent infections, and consequently, was pivotal in distinguishing a different exposure pattern between the bighorn sheep and cattle in an adjacent herd. Based on an increasing seroprevalence (50% to 100%), BTV circulated through this bighorn sheep population between 1989 and 1993. This increase in seroprevalence coincided with a bighorn die-off due to BTV infection in June, 1991. An adjacent cattle herd was sampled in 1995 for comparison. The bighorn sheep and adjacent cattle had different patterns of exposure to BTV between 1994 and 1995. There was no evidence that BTV circulated through the bighorn sheep population from 1994 to 1995. In 1995, seroprevalence to BTV decreased to 72%, none of yearling bighorn was seropositive, and all of the 39 bighorn sheep were PCR-negative. In contrast, all adult cattle were seropositive to BTV by c-ELISA and SN, and 4 of the calves were seropositive; 11 of the 24 cattle were PCR-positive, including all five calves. Overall, the pattern of temporal herd immunity in the bighorn sheep appeared to follow a classic epidemic curve, with the appearance and subsequent disappearance of herd immunity coinciding with the 1991 die-off in this population. As low levels of herd immunity and high proportions of susceptible animals are key factors in the development of epidemics, this population of bighorn sheep may be at increased risk for a BTV epidemic in the future.  相似文献   

17.
Browsing camels have access to different plant species in relation to the kind of pasture they can reach. A study was conducted in an arid region of Southern Tunisia to determine the dietary preference and forage quality of free ranging camels. Foliage consisting of leaves from Limonium pruinosum, Retama raetam and Stipa tenacissima, was collected during the dry season to evaluate the chemical characteristics and nutritional value of these browse fodder species. The dietary preference was studied using 15 adult camels which were selected from a herd of 50 animals appropriately marked for identification. There was a significant difference in the chemical composition and nutritional value of plant species collected. Based on crude protein (CP) content and nutritional value, the three fodder species browsed can be recommended as good-quality food source for camels under pastoral management.  相似文献   

18.
为了探索尿素对饲料利用率的影响,以瘤胃瘘管羊为实验动物,采用尼龙袋法,研究了尿素浓度梯度对饲料在羊瘤胃内降解率和主要养分含量变化的影响。选择3只瘤胃瘘管羊为实验动物,实验设对照组和试验组,对照组未添加尿素,试验组尿素浓度分别设为1%、2%、3%、4%和5%,测定饲料在瘤胃内6、12和24h时的粗脂肪、粗纤维、灰分、粗蛋白、钙含量和总降解率。结果表明,尿素添加水平在2%和3%时,饲料的消化率显著高于对照组和5%尿素组,饲料蛋白质、纤维素和脂肪含量显著低于对照组和5%尿素组,说明适量添加尿素可促进饲料营养物质利用率;添加2%和3%尿素可促进饲料钙的前期利用率,添加3%和4%尿素可促进饲料灰分的前期利用率。  相似文献   

19.
Objective To determine the proportion and incidence of calves persistently infected with bovine pestivirus in calves (n = 1521) supplied to the Tick Fever Research Centre and to assess the test regime to detect calves persistently infected with bovine pestivirus.
Design Calves, 1 to 6 weeks old, selected for use in the production of the tick fever vaccine were collected from 21 properties in 56 separate groups between October 1990 and December 1996. Each group was examined for the presence of calves persistently infected with bovine pestivirus.
Procedure All calves were routinely tested for antibody to bovine pestivirus and bovine pestivirus antigen using a serum neutralisation test and an antigen-capture ELISA, respectively. Pooled lymphocyte samples from calves were also monitored for bovine pestivirus by inoculation of sheep. Whole herd testing was carried out in eight herds, using a serum neutralisation test as a screen test followed by an antigen-capture ELISA of cattle with a serum neutralisation test titre of less than 32.
Results Fourteen of the 1521 calves tested (0.9%), were detected as persistently infected and the incidence ranged from 0.0 to 3.0 % per year over 6 years. Persistently infected calves were found in 13 of the 59 groups and originated from 7 of the 21 herds used. In whole herd testing on the properties of origin, cattle persistently infected with bovine pestivirus were detected in four of the eight herds tested
Conclusions The proportion of calves persistently infected with bovine pestivirus is similar to that in other countries and indicates that bovine pestivirus could be a significant cause of economic loss in Australian cattle herds. In detecting calves persistently infected with bovine pestivirus, the combination of sheep inoculation, paired antigen-capture ELISA and serum neutralisation tests appeared to be highly sensitive and specific.  相似文献   

20.
饲料型四倍体刺槐叶粉饲用价值的比较研究   总被引：21，自引：4，他引：17  
测定了廊坊地区饲料型四倍体刺槐Robinia pseudoacacia叶粉的粗蛋白、粗纤维、钙、磷等常规营养成分的含量及18种氨基酸组分,运用必需氨基酸分、必需氨基酸指数及综合评价法对其营养价值及饲用价值进行评价.结果表明:饲料型四倍体刺槐叶粉的粗蛋白和18种氨基酸含量分别高达27.27%和18.55%,粗纤维含量为16.47%,属于优良的植物饲料蛋白源;其中12种必需氨基酸含量之和为10.67%,必需氨基酸分为68.1,氨基酸组成较平衡;对牛、猪、鸡、鸭、鹅、草鱼、对虾和羊8种动物的必需氨基酸指数均大于0.86,可作为这些动物的优良蛋白饲料.  相似文献   

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