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为明确云南省昆明市露地栽培条件下番茄斑萎病毒病的发生流行特征,于2014—2015年采用病害系统调查法结合病毒ELISA及RT-PCR检测方法研究露地栽培条件下由番茄斑萎病毒(tomato spotted wilt virus,TSWV)引起的病毒病发生规律及其重要寄主种类,并研究利用防虫网隔离蓟马对番茄斑萎病毒病的防控效果。结果表明:番茄斑萎病毒病在露地番茄主要种植期3—10月普遍发生,番茄苗期和移栽初期是该病毒病防控的关键期,带毒种苗调运是该病毒病的主要传播途径;田间多种茄科和菊科植物是TSWV的重要中间寄主。在田间,菊科寄主植物油麦菜、莴苣、鬼针草、牛膝菊上TSWV的检出率均较高,在42.53%～81.63%之间;茄科寄主植物中辣椒上TSWV的检出率最高,为41.99%,其次为马铃薯,TSWV检出率为27.78%,在番茄上TSWV的检出率为19.02%,因此生产中应对这些TSWV重要中间寄主给予更多关注和防控。应用防虫网能有效隔离蓟马,使番茄斑萎病毒病发病率和病情指数较对照分别降低了6.44个百分点和5.31,可有效降低番茄苗期及定植期斑萎病毒病的发生。 相似文献
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番茄斑萎病毒Tomato spotted wilt virus （TSWV）是我国进境植物检疫性有害生物,近年来相继在国内一些省份发现。利用 TSWV 的通用引物 NF302／NR575对从山东烟台地区收集的15份疑似感染番茄斑萎病毒病的番茄样品进行检测;进一步对特异引物 TSWV-NF2037／TSWV-NR2825扩增的 TSWV 的 N 基因序列克隆、测序,并对 N 基因片段编码氨基酸进行遗传距离及系统发育分析。结果表明,15份疑似样品中有4个样品扩增得到 TSWV 病毒片段;基于 N 基因序列分析发现,山东 TSWV 番茄分离物与云南 TSWV 番茄分离物（AEI70836．1）的遗传距离最近,为0．8％,且与云南 TSWV 番茄分离物聚为一支。这是山东地区首次利用分子标记证实番茄斑萎病毒病的危害。 相似文献
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病毒病对番茄生产造成严重危害, 近年来在番茄种植新区发生严重, 疑似为种子带毒传播。本研究通过对云南省怒江州番茄种植新区的番茄病毒病样品采用RNA-seq高通量测序, RT-PCR验证的方法检测病毒种类;对番茄病果种子进行超薄切片制样透射电子显微镜观察, 将病果种子播种后对种苗进行RT-PCR带毒检测。结果表明, RNA-seq高通量测序及RT-PCR检测到的病毒有番茄环纹斑点病毒(tomato zonate spot orthotospovirus, TZSV)、番茄黄斑驳相关病毒(tomato yellow mottle-associated virus, TYMaV)、辣椒脉斑驳病毒(chili veinal mottle virus, ChiVMV)、南方番茄病毒(southern tomato virus, STV)。透射电镜观察到种胚细胞及胚乳细胞中分布典型的正番茄斑萎病毒属Orthotospovirus病毒粒体。病果种子播种28 d后的种苗具有病毒病症状, 通过RT-PCR检出TZSV、ChiVMV、STV, 检出率分别为60%、100%、80%。上述研究结果为TZSV通过种子传播提供了有利的证据, 并为源头防控番茄病毒病提供了依据。 相似文献
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正番茄斑萎病毒属病毒(orthotospoviruses)是严重危害云南蔬菜等重要农业经济作物的病毒病原之一。采用血清学检测、小RNA深度测序以及RT-PCR验证相结合的方法,从云南省昆明市晋宁区的主要作物寄主(番茄、辣椒、油麦菜)、重要中间寄主(鬼针草)和传毒介体(蓟马)中鉴定到TSWV、TZSV、PCSV和INSV 4种病毒,其中TSWV为该地区的主要优势病毒,而PCSV则是首次报道侵染鬼针草。通过对云南番茄斑萎病毒病害重病区作物寄主、中间寄主及蓟马三者进行病毒种类分析研究,明确TSWV为引起云南省昆明市晋宁区作物的主要病毒,TZSV、PCSV和INSV零星发生于不同寄主中。 相似文献
5.
山东烟台地区发生番茄斑萎病毒病危害 总被引：3，自引：0，他引：3
番茄斑萎病毒Tomato spotted wilt virus(TSWV)是我国进境植物检疫性有害生物,近年来相继在国内一些省份发现。利用TSWV的通用引物NF302/NR575对从山东烟台地区收集的15份疑似感染番茄斑萎病毒病的番茄样品进行检测;进一步对特异引物TSWV-NF2037/TSWV-NR2825扩增的TSWV的N基因序列克隆、测序,并对N基因片段编码氨基酸进行遗传距离及系统发育分析。结果表明,15份疑似样品中有4个样品扩增得到TSWV病毒片段;基于N基因序列分析发现,山东TSWV番茄分离物与云南TSWV番茄分离物(AEI70836.1)的遗传距离最近,为0.8%,且与云南TSWV番茄分离物聚为一支。这是山东地区首次利用分子标记证实番茄斑萎病毒病的危害。 相似文献
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辣椒是云南省主要经济作物之一,近年来病毒病尤其是正番茄斑萎病毒属病毒发病严重,影响了辣椒产量和品质。利用RT-PCR技术对从云南辣椒主产区采集的疑似感染正番茄斑萎病毒属病毒的25份辣椒样品进行分子鉴定,结果显示,12份样品检测出正番茄斑萎病毒属病毒,检出率为48.0%,其中6份是番茄斑萎病毒tomato spotted wilt orthotospovirus (TSWV),检出率为24.0%;5份是番茄环纹斑点病毒tomato zonate spot orthotospovirus (TZSV),检出率为20.0%;有1份是TSWV和TZSV复合侵染,检出率为4.0%,这是在云南辣椒生产上首次发现TSWV和TZSV的复合侵染。通过鉴定,初步了解正番茄斑萎病毒属病毒在云南辣椒生产中的发生情况和种类,为制定云南地区该属病毒的防治策略提供理论依据。 相似文献
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调查发现北京地区一温室栽培茄子Solanum melongena L.出现严重病毒病。利用基于小RNA的高通量测序技术和RT-PCR方法,明确了引起茄子病害的病毒种类为番茄斑萎病毒,将其命名为TSWV-eggplant分离物。进一步克隆了该病毒的基因组全长(S RNA、M RNA、L RNA),并构建其系统发育树。结果表明,该分离物的S RNA与美国分离物亲缘关系较近,M RNA与中国分离物亲缘关系较近,而L RNA与韩国分离物亲缘关系较近。因此,本研究发现的TSWV分离物与国内已发生报道的分离物不同,该分离物是否存在不同分离物之间基因组的重组需要进一步研究。 相似文献
10.
番茄斑萎病毒Tomato spotted wilt tospovirus(TSWV)是严重危害世界经济作物的一种病毒,寄主范围广泛。我们在研究中发现番茄斑萎病毒能侵染我国大蒜Allium sativum L.。利用摩擦接种法将TSWV接种到健康大蒜上,结果显示:接种14 d后大蒜新生叶片出现褪绿和白色斑点症状。ELISA检测显示大蒜叶片汁液与TSWV的单克隆抗体产生血清学反应,采用TSWV N基因的引物对大蒜叶片总RNA进行RT-PCR,结果扩增出约800 bp的条带,在NCBI上BLAST显示与TSWV YN5576的同源性最高,为98.52%。这些数据表明番茄斑萎病毒系统侵染我国大蒜。 相似文献
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广东番茄上检测到Tospovirus病毒 总被引：1，自引：0，他引：1
Some tomato samples possibly infected by tospovirus in Guangdong were detected with indirect ELISA and RT-PCR. The results showed that the virus infected tomato did not react with the antiserum of Tomato spotted wilt virus (TSWV), but about 500 bp fragment of RT-PCR shared 83%-84% nucleotide identities with N gene of those reported tospoviruses. The phylogenetic tree of the N gene fragment compared with those of other tospoviruses indicated that the virus infected tomato was belonged to Tospovirus. 相似文献
13.
Eduardo Segundo María P. Carmona Elisa Sáez Leonardo Velasco Germán Martín Leticia Ruiz Dirk Janssen Isabel M. Cuadrado 《European journal of plant pathology / European Foundation for Plant Pathology》2008,122(4):579-591
A study was conducted to determine the identity and prevalence of viruses in 455 greenhouses in the main Spanish green bean
growing area. Directed surveys were conducted in 422 crops from 2000–2004 to collect samples from diseased plants displaying
symptoms that could be attributed to viruses. The samples were analysed to detect any virus by means of dsRNA extraction,
mechanical inoculation to test plants, as well as ELISA and/or RT-PCR tests to detect potyviruses, geminiviruses and viruses
previously known to infect beans in Spain. Random surveys were conducted in the years 2002 and 2005 (in 21 and 12 greenhouses,
respectively) to study the actual incidence of known viruses in the area. Symptoms were recorded in 23,108 plants from which
664 plants were collected and analysed by ELISA or RT-PCR. The results of the directed surveys showed that all the analyzed
crops carried the cryptic virus Phaseolus vulgaris endornavirus (PVuV), whereas phytopathogenic viruses appeared in smaller percentages of the crops: Tomato yellow leaf curl virus (TYLCV) 20.4%, Southern bean mosaic virus (SBMV) 9.0%, Tomato spotted wilt virus (TSWV) 4.0%, and the new species Bean yellow disorder virus (BnYDV) that broke out in 2004 with occurrence values higher than 34.3% that year. From 2000–2004 an important decrease in
TYLCV was observed, along with a slight increase in SBMV and a consistently low occurrence of TSWV. The results of the random
surveys confirmed the increased occurrence of virus detected during the directed surveys, and furthermore demonstrated the
percentage of incidence for each virus. 相似文献
14.
Synergistic Interaction Between Tomato chlorosis virus and Tomato spotted wilt virus Results in Breakdown of Resistance in Tomato 总被引：1，自引：0，他引：1
ABSTRACT Multiple viral infections frequently are found in single plants of cultivated and wild hosts in nature, with unpredictable pathological consequences. Synergistic reactions were observed in mixed infections in tomato plants doubly infected with the positive-sense and phloem-limited single-stranded RNA (ssRNA) crinivirus Tomato chlorosis virus (ToCV) and the negative-sense ssRNA tospovirus Tomato spotted wilt virus (TSWV). Synergism in a tomato cultivar susceptible to both viruses resulted in a rapid death of plants. A pronounced enhancement of ToCV accumulation mediated by TSWV co-infection was observed with no evident egress of ToCV from phloem tissues. No consistent alteration of TSWV accumulation was detected. More remarkable was the synergism observed in tomato cultivars which carry the Sw-5 resistance gene, which are resistant to TSWV. Pre-infection with ToCV resulted in susceptibility to TSWV, whereas co-inoculations did not. This suggested that a threshold level or a time lapse is needed for ToCV to interfere or downregulate the defense response in the TSWV-resistant plants. 相似文献
15.
黑龙江地区番茄斑萎病毒的鉴定及其部分生物学特征分析 总被引：2，自引：0，他引：2
近年来,番茄斑萎病毒Tomato spotted wilt tospovirus (TSWV)在我国多个地区发生严重危害。本研究采用小RNA深度测序和RT-PCR相结合的方法对采自黑龙江的番茄病毒病样品中的病毒进行了鉴定。提取番茄样品的RNA并构建小RNA文库进行测序,数据分析发现比对至TSWV基因组的reads数占比对到病毒核酸总reads数的92.64%,表明侵染此番茄样品的病原物可能是TSWV。利用RT-PCR方法进一步确定侵染此番茄样品的病毒为TSWV,将其命名为TSWV-HLJ1。对TSWV-HLJ1的核苷酸序列进行分析并构建系统进化树,结果表明TSWV-HLJ1与TSWV云南甜椒分离物(TSWV-YNgp)的亲缘关系最近。介体传毒试验表明,西花蓟马可将TSWV-HLJ1传播感染健康寄主植物。摩擦接种试验表明,TSWV黑龙江分离物能够侵染本氏烟、辣椒和番茄。这是我国首次报道在黑龙江地区发现TSWV的危害。 相似文献
16.
Y. Antignus M. Lapidot N. Ganaim J. Cohen O. Lachman M. Pearlsman B. Raccah A. Gera 《Phytoparasitica》1997,25(4):319-330
Received April 24, 1997; received in final form June 29, 1997. Symptoms resembling tomato spotted wilt virus (TSWV) infections
were documented among ornamental and vegetable crops in commercial greenhouses and open fields in Israel. Plants exhibiting
these symptoms were collected from January 1992 to December 1996. Among cultivated plants analyzed for TSWV by enzyme-linked
immunosorbent assay (ELISA), 19 species representing five families were found to be infected; natural infection was also recorded
in six plant species of weeds. Virus identity was characterized by host range, serology and electron microscopy. Serological
reaction with the isolates, found in Israel, using antisera from different sources as well as the sequence analysis of the
nucleocapsid gene, demonstrated that the Israeli isolates of TSWV are a member of tospovirus serogroup I, type I (BR-01 strain).
No virus transmission was found in seeds collected from virus-infected vegetable and ornamental crops. A non-radioactive molecular
probe derived from the cloned nucleocapsid isolate enables specific detection of the virus in crude sap from infected plants.
The detection of TSWV in Israel constitutes a severe potential threat to the ornamental and vegetable industry. 相似文献