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1.
为探明不同茬口番茄光合特性对土壤水分响应机制,采用盆栽方式研究50%、65%和80%土壤水分处理对冬春两茬番茄叶片PSⅡ光化学活性和光能分配的影响。结果表明,随着土壤相对含水量减少,春茬番茄叶片的叶绿素含量下降,电解质渗透率增加,叶片最大光化学效率(F_v/F_m)、实际光化学效率(ΦPSⅡ)、电子传递速率(ETR)、光化学猝灭系数(q P)和吸收光能用于进行光化学反应份额(P)降低,非光化学猝灭(NPQ)、双光系统间激发能分配不平衡偏离系数(β/α-1)、天线热耗散份额(D)和非光化学猝灭量子产量[Y(NO)]显著升高,表明叶片用于PSⅡ反应中心的光化学活性降低,用于非化学反应的能量增加,叶片发生光抑制和光损伤程度增加,且与水分亏缺程度呈显著正相关,春茬番茄以80%土壤含水量表现较好。冬茬番茄以50%水分处理的F_v/F_m、ΦPSⅡ、ETR、q P和P较低,但生长中后期时,65%水分处理的F_v/F_m、ΦPSⅡ、ETR、q P和P却显著高于80%处理,与充足供水相比,轻度水分胁迫下的番茄叶片的光抑制发生程度较轻。综上可知,春茬番茄以80%水分处理的叶片光化学活性较高,而冬茬番茄以65%水分处理表现较好。本研究结果为不同茬口番茄生产的合理灌溉提供了理论依据。  相似文献   

2.
张绪成  上官周平 《核农学报》2007,21(3):299-304,310
在田间试验条件下,研究了不同抗旱性小麦品种全生育期叶片光合色素含量和叶绿素荧光参数的关系及其氮素响应。结果表明,光合色素各组分含量随施氮量增加有明显的升高趋势,品种间差异又因生育时期而不同;施氮显著提高了拔节期实际光化学效率(ФPSⅡ)和光化学猝灭系数(qP)、扬花期和灌浆期的最大光化学效率(Fv/Fm),降低了全生育期非光化学猝灭系数(qN)。提高叶绿素a含量能显著提高叶片ФPSⅡ、Fv/Fm、qP和降低qN;叶绿素b含量的升高能显著增强热耗散,增加类胡萝卜素含量则促进水地品种叶片光能的光合碳同化作用和旱地品种的热耗散。拔节期对照处理的旱地品种的ФPSⅡ和qN显著高于水地品种,在该时期品种抗旱性差异表现较为明显,能够通过提高光合机构实际光化学效率和热耗散来增强光合机构对干旱的适应能力。  相似文献   

3.
为合理利用黄土丘陵区优良乡土草种,探索人工草地建设中乡土草种的最佳混播比例,选取白羊草(Bothriochloa ischaemum)和达乌里胡枝子(Lespedeza davurica)为研究材料,采用生态替代法,按照白羊草与达乌里胡枝子行比设置了7种组合比例(即0∶10,2∶8,4∶6,5∶5,6∶4,8∶2和10∶0),比较研究了丰水年(2011年,降雨量663.4mm)与偏旱年(2012年,降雨量458.2mm)混播草地土壤水分季节变化特征、年度生物量和水分利用效率。结果表明,白羊草与达乌里胡枝子混播草地土壤水分消耗和补充与混播比例密切相关,但主要受年度降雨量及其季节分配的影响。在2011年,充沛的降雨可补充20—260cm土层土壤水分,并弱化了不同混播比例和土层间土壤水分的时空差异。不同降雨量年份里,白羊草和达乌里胡枝子在6∶4与8∶2两混播比例下的地上生物量和水分利用效率均显著较高。  相似文献   

4.
耐弱光基因型马铃薯在遮阴条件下的光合和荧光特性分析   总被引:1,自引:1,他引:0  
为探寻遮阴对马铃薯光合和荧光特性及吸收光能分配的影响,本研究采用大田试验,以马铃薯不耐弱光品种‘丽薯6号’和耐弱光品种‘中薯20’为材料,出苗后用遮光率70%的黑色遮阳网进行遮阴处理(T),以自然光照为对照(CK),测定了遮阴30 d后马铃薯叶片的光合作用、光响应曲线、CO_2响应曲线、光诱导曲线和叶绿素荧光参数的变化。结果表明:1)遮阴后净光合速率(P_n)、气孔导度(G_s)、蒸腾速率(T_r)、最大净光合速率(P_(max))、光饱和点(LSP)、光补偿点(LCP)较CK显著下降;‘中薯20’的P_n、P_(max)、LSP较高,LCP和暗呼吸速率(R_d)较低。2)不同基因型CO_2响应参数无显著差异,但‘中薯20’的最大羧化速率(V_(cmax))和最大电子传递速率(Jmax)较高,CO_2补偿点(CCP)较低。3)高光诱导过程中,‘中薯20’反应较快,光合能力较强。4)初始荧光(F_o)、最大荧光(F_m)、最大光化学量子效率(F_v/F_m)较CK显著增加,PSⅡ实际光化学量子效率(?F/F_m′)、表观电子传递速率(ETR)和光化学猝灭系数(qP)较CK显著下降,非光化学猝灭系数(NPQ)呈增加趋势。5)非光化学热耗散(ФNPQ)和荧光耗散途径(Фf,d)比例显著增加,光化学猝灭耗散途径(ФPSⅡ)比例显著减少,主要以增加热耗散为主。遮阴后,耐弱光基因型‘中薯20’的NPQ和ФNPQ均高于不耐弱光的‘丽薯6号’,说明‘中薯20’的光合机构保护能力更强。综合分析表明,遮阴后耐弱光基因型马铃薯具有较高的净光合速率、较低的光补偿点、较低的CO_2补偿点、较快的光诱导反应速度和较高的非光化学热耗散能力。  相似文献   

5.
全生育期UV-B辐射增强对棉花生长及光合作用的影响   总被引:2,自引:1,他引:2  
植物光合系统是UV-B辐射最初和最重要的作用靶标。本文在大田条件下进行紫外灯照射处理,研究全生育期UV-B辐射增强(高于环境20%和40%)对棉花形态、干物质积累、光合色素和产量的影响,并通过分析棉花主茎功能叶片的气体交换参数和叶绿素荧光参数,探讨UV-B辐射增强影响棉花光合作用的机制。结果表明,UV-B辐射增强抑制了棉花生长和干物质积累,籽棉产量显著降低,且UV-B辐射越强,抑制作用越明显。随UV-B辐射的增强,棉花主茎功能叶的净光合速率(P_n)在各生育期均显著降低,叶绿素含量呈先升高后降低趋势,气孔导度(Gs)和蒸腾速率(Tr)未发生变化,胞间CO_2浓度(Ci)反而升高,说明P_n下降主要由非气孔限制因素造成。对叶绿素荧光参数的分析表明,PSⅡ的最大光化学量子产率(F_v/F_m)、实际光化学量子效率(ΦPSII)、线性电子传递速率(ETR)和光化学淬灭系数(qP)随着UV-B辐射的增强而降低,非光化学猝灭系数(NPQ)则显著升高,且各叶绿素荧光参数与Pn变化均显著相关;慢速弛豫NPQ(NPQS)及其在NPQ中的比例均随UV-B辐射的增强而显著提高,表明PSⅡ反应中心受损,光化学效率降低。以上结果证明,全生育期UV-B辐射增强降低了棉花的光合叶面积、叶绿素含量和净光合速率,引起棉花生长与物质积累受抑,产量降低。UV-B辐射增强引起的光合速率下降与PSⅡ反应中心遭到破坏密切相关。  相似文献   

6.
为了研究土壤水分与植物生长间的关系,以侧柏为材料进行盆栽试验,人工控制土壤水分,设置4个水分梯度(正常供水、轻度水分胁迫、中度水分胁迫、重度水分胁迫),测定其叶温和叶绿素荧光参数.结果表明:随着干旱胁迫的加剧,叶温和非光化学淬灭升高;初始荧光F0总体上表现为逐渐上升的趋势;而最大荧光Fm、可变荧光Fv、PSⅡ最大原初光化学量子效率Fv/Fm、PSⅡ的潜在活性Fv/F0、PSⅡ实际光化学量子效率和表观光合电子传递速率均表现为下降.参数的日变化呈现单峰型曲线:PSⅡ实际光化学量子效率和非光化学淬灭的日变化曲线为凹形,而表观光合电子传递速率日变化曲线为凸形.可知干旱胁迫导致PSⅡ的电子传递效率降低,降低了光能利用率,影响其光合作用.  相似文献   

7.
通过水培试验, 研究了外源NO供体硝普钠(SNP)处理对100 mmol·L-1 NaCl胁迫下番茄幼苗叶绿素荧光特性的影响.结果表明, 外源NO提高了盐胁迫下番茄幼苗叶片的最大荧光(Fm)、PSⅡ最大光化学效率(Fv/Fm)、PSⅡ潜在活性(Fv/Fo)、表观光合电子传递速率(ETR)、实际光化学效率(ΦPSⅡ)、光化学荧光猝灭系数(qP)和光化学速率(Prate), 降低了初始荧光(Fo)、非光化学荧光猝灭系数(qN)、天线热耗散速率(Drate)和PSⅡ激发能压力(1-qP), 同时降低了激发能在两个光系统间的分配不平衡性.表明外源NO通过减少过剩激发能的耗散, 提高光合电子传递效率, 可有效缓解盐胁迫对番茄PSⅡ系统的伤害, 进而在缓解盐胁迫中发挥重要作用.  相似文献   

8.
谷子对拔节期弱光胁迫的光合生理响应   总被引:5,自引:0,他引:5  
为阐述拔节期弱光胁迫降低谷子产量的光合生理机制,以张杂谷5号和晋谷21号为研究对象,在拔节期分别进行30%、60%和85%的遮阴处理15 d,以不遮阴作为对照,探究谷子叶片的光合色素含量、光合特性、叶绿素荧光参数及产量对其的响应规律。结果表明,遮阴处理降低了2个品种谷子叶片的光合色素含量、净光合速率(Pn)、蒸腾速率(Tr)、气孔导度(Gs)、表观光合电子传递速率(ETR)、PSⅡ实际光化学量子产量(ΦPSⅡ)、光化学猝灭系数(q P)、非调节性能量耗散量子产量[Y(NO)]、穗长、穗重、穗粒重和产量,却显著提高了胞间CO2浓度(Ci)和调节性能量耗散量子产量[Y(NPQ)]。张杂谷5号的表观光合电子传递速率(ETR)、PSⅡ实际光化学量子产量(ΦPSⅡ)和产量在遮阴30%时显著下降,而晋谷21号在遮阴程度超过60%时才显著变化。总之,拔节期弱光胁迫下,谷子叶片的光合色素含量降低,捕捉利用光能的能力减弱,PSⅡ光化学活性降低,光合作用变弱,最终影响产量;张杂谷5号比晋谷21号对弱光胁迫更为敏感。本研究结果为拔节期通过改善谷子叶片的光合功能来增强抗弱光胁迫以达到稳产指明了方向,并为选育耐弱光胁迫谷子提供了依据。  相似文献   

9.
水分胁迫对茶树叶片叶绿素荧光特性的影响   总被引:12,自引:0,他引:12  
以"铁观音"和"福鼎大白茶"两个茶树品种为试验材料,研究水分胁迫对茶树叶片叶绿素荧光特性的影响.结果表明:水分胁迫下,茶树叶片的基础荧光(Fo)显著升高,最大荧光(Fm)、可变荧光(Fv)、PSⅡ原初光能转化效率(Fv/Fm)、PSⅡ的潜在活性(Fv/Fo)显著下降,表明水分胁迫使茶树叶片PSⅡ反应中心受到伤害;水分胁迫还可降低茶树的光化学反应速率(Prate),缩短荧光上升时间(T1/2),抑制PSⅡ反应中心电子的传递(ETR降低),导致天线色素热耗散速率(Drate)、非光化学猝灭(qN)和光合功能相对限制值(LPFD)升高,光化学猝灭(qP)和PSⅡ实际光化学效率(Yield)显著下降.比较水分胁迫下"铁观音"和"福鼎大白茶"叶片叶绿素荧光变化发现,"铁观音"PSⅡ反应中心的胁迫耐性较强,叶片光化学效率较高.  相似文献   

10.
以大花生品种606为材料,选用N-P2O5-K2O含量相同的普通复合肥和控释复合肥,设置不施肥(CK)、拔节期施普通复合肥(JCF)、拔节期施控释复合肥(JCRF)、挑旗期施普通复合肥(FCF)、挑旗期施控释复合肥(FCRF)5个处理,研究了不同肥料类型及施肥方式对麦套花生叶片叶绿素含量、叶绿素荧光参数、根系活力、硝酸还原酶活性和抗氧化活性的影响。结果表明:与普通复合肥处理相比较,控释复合肥处理的F_v/F_m、F_v/F_o和ΦPSⅡ在结荚期之前差异不显著,但在饱果期与成熟期显著高于JCF处理;根系活力与硝酸还原酶活性的变化规律与F_v/F_m、F_v/F_o和ΦPSⅡ基本一致;控释复合肥处理的SOD、POD和APX在花生苗期无明显差异,但在中后期显著升高,丙二醛(MDA)含量显著降低,且小麦挑旗期追肥处理效果优于拔节期追肥。控释复合肥处理对小麦产量无明显影响,但显著提高了花生产量,JCRF处理的荚果产量和籽仁产量较JCF分别提高5.1%和7.6%,FCRF较FCF处理提高5.9%和8.0%。表明在施NP2O5-K2O等量的条件下,控释复合肥能够满足花生生长后期对养分的需求,改善叶肉细胞的光合性能,增强根系活力和NR活性,促进根系对养分的吸收。同时,提高中后期清除活性氧的能力,有助于延缓叶片衰老,从而提高麦套花生产量。两类型肥料对小麦和花生两种作物产量的提高均以挑旗期追肥效果最佳,拔节期追肥次之。  相似文献   

11.
Nonpathogenic binucleate Rhizoctonia fungi (BNR) controlled black shank caused by Phytophthora parasitica var. nicotianae on greenhouse-grown tobacco seedlings in styrofoam float trays. Three BNR isolates were incorporated into a soil-less mix on colonized, pulverized, sifted rice particles; colonized whole rice grains; or on pelleted tobacco seeds coated with 0.5% methyl cellulose. Five-wk-old seedlings were inoculated with zoospores of P. parasitica var. nicotianae and disease rated over 10 d. The level of protection varied with method of BNR application, ranging from 40 to 70%. Overall, control was better when BNR isolates were applied on rice inocula rather than on BNR-colonized tobacco seeds. From 15% to 80% of individual roots from seedlings grown in soil-less mix amended with BNR-colonized rice grains were colonized while only 0–20% of roots from seedlings grown from BNR-colonized tobacco seeds were colonized. Likewise, 37–100% of soil-less mix amended with BNR-colonized rice grains contained BNR's while less than 3% of soil-less mix was colonized when seedlings emerged from BNR-colonized tobacco seeds. This is the first demonstration of biocontrol of Phytophthora by BNR fungi.  相似文献   

12.
茄二十八星瓢虫对雷公藤和曼陀罗提取物的敏感性   总被引:1,自引:0,他引:1  
为研究寄主植物对茄二十八星瓢虫药剂敏感性及体内酶活力的影响,采用浸渍法分别测定了取食茄子、马铃薯、番茄和龙葵的茄二十八星瓢虫幼虫(以下简称幼虫)对雷公藤和曼陀罗提取物的敏感性,用生化方法测定了雷公藤和曼陀罗提取物对幼虫体内乙酰胆碱酯酶(AChE)和主要解毒酶活性的影响。结果表明,雷公藤提取物对取食茄子、马铃薯、番茄和龙葵的幼虫LC50值分别为1.407 9 mg.L-1、1.595 8 mg.L-1、1.464 7 mg.L-1和1.109 7 mg.L-1,相对毒力指数为78.82、69.54、75.76和100;曼陀罗提取物对取食茄子、马铃薯、番茄和龙葵的幼虫LC50值分别为0.641 7 mg.L-1、0.610 3 mg.L-1、0.758 0 mg.L-1和0.488 3 mg.L-1,相对毒力指数为76.09、80.01、64.42和100;取食龙葵的幼虫对2种植物提取物的敏感性显著高于取食茄子、马铃薯和番茄的幼虫。取食4种寄主植物的幼虫体内靶标酶AChE和主要解毒酶谷胱甘肽-S-转移酶(GST)、羧酸酯酶(CarE)、多功能氧化酶(MFO)活力顺序均为茄子>马铃薯>番茄>龙葵。其中,取食番茄和龙葵的幼虫体内AChE和GST活力显著低于取食茄子和马铃薯的幼虫,取食马铃薯、番茄和龙葵的幼虫CarE活力显著低于取食茄子的幼虫,取食4种寄主植物的幼虫MFO活力差异达极显著水平。雷公藤和曼陀罗提取物对幼虫体内AChE和GST、CarE和MFO活性均有抑制作用。其中,对取食马铃薯幼虫的AChE活力抑制作用最强,其次为取食茄子和番茄的幼虫,对取食龙葵的幼虫抑制作用最低。雷公藤和曼陀罗提取物对取食龙葵幼虫CarE活力的抑制作用最强,而对取食马铃薯的幼虫MFO活力的抑制作用最强。寄主植物中的次生物质对解毒酶的诱导或抑制可能是引起药剂敏感性差异的主要原因。  相似文献   

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14.
15.
Christ‘s thorn (Ziziphus spina-christi L. Desf.) is ecologically and economically important in Iran. Since it is a crosspollinated plant with a wide range of genetic variability, an investigation was carried out to determine the best method for mass and clonal propagation. Three vegetative methods were examined, including 1) cutting, where shoots with a 22-25 cm length and three shoot diameters were treated with two culture media; 2) layering, where air layering and trench layering treatments consisted of three growth regulators at three levels of concentration; and 3) tissue culture with nodal segments bearing axillary buds that were removed from shoots of mature trees at different seasons. Several experiments were carried out to determine the best disinfectant chemical, the best culture method, the best season, the appropriate explant characteristics and media type. Rooting was successful only on the sandy beds for cuttings with more than 8.0 mm diameter. For the trench layering method, only one specimen in one replicate rooted a very small root, while for the air layering method, only seedlings with growth regulator treatments rooted successfully. The best season for explant harvesting was determined as mid summer, and among the disinfecting treatments, Ca(OCI)2 at a concentration of 0.5g kg^-1 for 20 minutes was the best. There were no significant differences for shoot weight and length with a Murashige and Skoog (MS) medium with and without a hormone.  相似文献   

16.
A greenhouse experiment was performed to evaluate the influence of Rhizobium when co-inoculated with each of two Paenibacillus polymyxa strains, singly and in mixture on growth, nitrogen content, phytohormone levels and nodulation of the common bean (Phaseolus vulgaris L.) under three levels of drought stress. Stress was applied continuously by the control of matric potential (ψm) through a porous cup. Bean plants cv. Tenderlake were grown in pots with Fluvic Neosol eutrophic soil under three different ψm (S1 −7.0; S2 −70.0 and S3 < −85 kPa). The seeds were inoculated with Rhizobium tropici (CIAT 899) and each of P. polymyxa (DSM 36) and P. polymyxa Loutit (L) singly and in mixture (CIAT 899 + DSM36 + Loutit). Co-inoculation of bean with Rhizobium and both Paenibacillus strains resulted increased plant growth, nitrogen content and nodulation compared to inoculation with Rhizobium alone. This was particularly evident at the most negative ψm (S3 < −85 kPa) we used. Drought stress triggered a change in phytohormonal balance, including an increase in leaf abscisic acid (ABA) content, a small decline in indole acetic acid (IAA) and gibberellic acid (GA3) and a sharp fall in zeatin content in bean leaves. The content of endogenous Cks decreased under water stress, possibly amplifying the response of shoots to increasing ABA content. We hypothesize that co-inoculation of bean with R. tropici (CIAT 899) and P. polymyxa strains (DSM 36) and Loutit (L) mitigates some of the negative effects of drought stress on bean.  相似文献   

17.
A pot experiment was conducted to investigate the mobilization of sparingly soluble inorganic andorganic sources of phosphorus (P) by red clover (Trghlium pmtense L.) whose roots were colonized by the arbuscular mycorrhizal (AM) fungus Glomus mosseae and in association with the phosphate-solubilizing (PS) bacterium Bacillus megaterium ACCC10010. Phosphate-solubilizing bacteria and rock phosphate hada synergistic effect on the colonization of plant roots by the AM fungus. There was a positive interaction between the PS bacterium and the AM fungus in mobilization of rock phosphate, leading to improved plant P nutrition. In dual inoculation with the AM fungus and the PS bacterium, the main contribution to plant P nutrition was made by the AM fungus. Application of P to the low P soil increased phosphatase activityin the rhizosphere. Alkaline phosphatase activity was significantly promoted by inoculation with either the PS bacterium or the AM fungus.  相似文献   

18.
The determinants of saprotrophic or predatory modes of the nematophagous fungus Arthrobotrys oligospora were investigated in soil microcosms and on solid nutrient media. A sterilized soil amended with 1% w/w alfalfa meal (C:N=32) and inoculated with conidia of A. oligospora, showed lower mycelium biomass and higher specific rate of conidia production in the presence of the bacterivorous nematode Caenorhabditis elegans than in its absence. As few as 10 nematodes g−1 soil were sufficient to enhance spore formation by the vegetative mycelium. Given that the fungus was not limited by available carbon and nitrogen, this indicates that nematodes provide essential growth factors regulating the development of A. oligospora. Carbon mineralisation by A. oligospora, measured as the rate of CO2 production, was found to be 25–35% lower in the presence of 20–60 C. elegans g−1 soil compared to soil without nematodes. This showed that A. oligospora had lower saprotrophic activity in the predaceous phase. Trap formation and nematophagous activity of A. oligospora were observed only where conidia were inoculated on nutrient poor medium (water agar), on low-nitrogen medium (Yeast Carbon Base agar) or on medium containing no amino-acids or vitamins (Czapek-Dox agar). A. oligospora did not form trapping structures when grown on nutrient-rich media containing three amino-acids (l-histidine monohydrocloride, dl-methionine and dl-tryptophan) and vitamins (biotin, calcium pantothenate, folic acid, inositol, niacin, p-aminobenzoic acid, pyridoxine hydrochloride, riboflavine, thiamine hydrochloride). It is concluded that predaceous behaviour of A. oligospora can be regulated either by nitrogen sources or by physiologically active compounds (amino-acids or vitamins) present in nematodes.  相似文献   

19.
采用悉生培养系统,研究不同浓度菲对秀丽隐杆线虫、拟丽突属与中杆属线虫的毒性效应,以及3种线虫对菲的去除作用。结果表明:(1)随着菲浓度的增加,3种线虫存活率逐渐降低。秀丽隐杆线虫在不添加菲的处理中,48 h内出现繁殖,而在添加菲的处理中,即使在最低浓度5 mg/L下,繁殖现象也会消失。中杆属与拟丽突属线虫由于世代时间较长,在本试验周期内均未出现繁殖现象。(2)暴露24 h时,比较不同浓度菲处理下线虫的相对死亡率,得到3种线虫的耐性依次为中杆属线虫≥秀丽隐杆线虫≥拟丽突属线虫,且随菲浓度的增加,秀丽隐杆线虫耐性水平逐渐降低;暴露48 h时中杆属线虫耐性依旧高于拟丽突属,而72 h时中杆属与拟丽突属线虫的耐性趋于一致。(3)3种线虫受菲胁迫后均失去头部正常摆动能力,且秀丽隐杆线虫与拟丽突属线虫体长随菲浓度的增加而逐渐降低。(4)不同种类线虫的添加均能促进菲的去除,不同线虫之间无显著差异。因此,菲会显著抑制3种线虫的存活率和生长发育,抑制秀丽隐杆线虫的繁殖。线虫的存活率受线虫种类、暴露时间、菲浓度及其交互作用的影响显著,其中中杆属线虫对菲的综合耐性最强,3种线虫均能促进溶液中菲的去除。  相似文献   

20.
A 5-year study on the soil ecological environment of matsutake(Tricholoma Matsutake)growing areas near Changbai Mountains showed that several factors,such as climate,parent material,organism and landform,which form the soil ecosystem provided sutable ecological environment for the formation and growth of matsutake How to improve the construction and function of the soil ecosystem in order to utilize the natural resource continumously was also studied.  相似文献   

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