共查询到18条相似文献，搜索用时 821 毫秒
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本实验对注射用盐酸罗沙替丁醋酸酯的细菌内毒素检查方法进行研究,建立快速准确的细菌内毒素检查法。按《中国药典》2010版附录ⅪE细菌内毒素检查方法进行。利用不同厂末生产的鲎试荆进行干扰试验,全面考察注射用盐酸罗沙替丁醋酸酯对细茼内毒素控查的干扰作用,以确定最佳试验条件。注射用盐酸罗沙替丁醋酸酯可以采用稀释方法排除干扰。本细茵内毒素检查结果安全、可靠。 相似文献
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对油剂普鲁卡因青霉素注射液细菌内毒素检查方法进行了研究,在样品中加入适量的无菌、无内毒素的０．１ｍｏｌ／Ｌ氢氧化钠％吐温－８０溶液;并用０．１ｍｏｌ／Ｌ盐酸调节样品液的ＰＨ值,解决了样品的溶解方法,。用稀释法排除样品对细菌内毒素检查的干扰。实验结果表明,选用鲎试剂灵敏度为０．５ＥＵ／ｍｌ将样品稀释 １２５００ｕ／ｍｌ的溶液后可消除其干扰作用,因此,用细菌内毒素检查法作为该药品的热原物质检查是可行持 相似文献
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按照《中国兽药典》二○○五年版一部[1]中注射用硫酸链霉素细菌内毒素检查法对样品复核检验,试验表明该方法是可行的;并与《美国药典》进行了比较,结果说明《中国兽药典》注射用硫酸链霉素细菌内毒素检查法对细菌内毒素的限值表达比《美国药典》29版[2]更合理、更科学。 相似文献
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参照《中国兽药典》2000版收载的细菌内毒素检查法，验证了硫酸卡那霉素注射液的细菌内毒素检查法。通过干扰试验证明，硫酸卡那霉素注射液对鲎试剂的凝集反应无干扰作用，用灵敏度为0．25EU／mL的鲎试剂检查细菌内毒素的方法可行、有效。可以用细菌内毒素检查法替代家兔热原检查法来检测硫酸卡那霉素注射液的热原。 相似文献
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杨秀玉 《中国预防兽医学报》2009,31(12)
为检测转移因子(TF)注射液中细菌内毒素,本研究采用鲎试剂与细菌内毒素产生凝集反应的凝胶法对国内1个生物制品厂的TF注射液样品进行了检测,实验结果表明当TF注射液稀释至0.025 mg/mL时,对鲎试剂的凝集反应无干扰作用.因此,该方法适用于TF注射液中细菌内毒素检测,并可以用于该项生物制品的质量监控. 相似文献
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目的：考察细菌内毒素检查法对葡萄糖酸钙注射液进行检查的可行性。方法：用细菌内毒素检测法对家兔热原试验的葡萄糖酸钙注射液进行比较试验。结果：供试品对鲎试剂与内毒素的反应无干扰作用。家兔热原检查法结果与细菌内毒素检查法结果相吻合。结论：用细菌内毒素检查法检测葡萄糖酸钙注射液热原是可行的。 相似文献
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抗菌药物治疗奶牛子宫内膜炎后牛奶中药物残留及其对酸奶发酵的影响 总被引：1，自引：0，他引：1
用TTC法检测44例经过抗菌药物治疗的患子宫内膜炎荷斯坦奶牛。其中，41例采用子宫内灌注药物治疗，主要药物有土霉素、金霉素和庆大霉素；每个病例治疗1～5次不等，3例采用静脉注射，主要药物有磺胺嘧啶钠和头孢噻啶。结果表明，31例经土霉素子宫内灌注的奶牛，停药后第24h所采的奶样均可检出抗生素；其中6头奶牛在第6d仍然抗生素阳性，残留时间最长的可达8d。8例用金霉素子宫灌注治疗的奶牛，停药后第48h所采的奶样，抗生素检出率100％，其在牛奶中的残留时间为2～5d不等。2例经庆大霉素子宫灌注治疗的患病牛，药残时间分别为1～2d。3头经静脉滴注治疗的病牛，牛奶中的药残时间约达到9d以上。此外，从3头用金霉素子宫内灌注的奶牛采集牛奶用于乳酸菌发酵试验。结果表明，牛奶TTC阳性时，发酵产酸较低。在治疗后6h采集的牛奶都不能正常发酵产酸、凝固。3例试验牛牛奶发酵产生的酸度分别于治疗后第13、24和48h恢复到治疗前的水平。 相似文献
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为严格控制盐酸土霉素可溶性粉的质量,对盐酸土霉素及其可溶性粉按标准中鉴别方法进行试验。结果发现：该方法鉴别（1）、（2）项存在疏漏之处,不能客观地评价产品的质量。通过查找原因和分析,对标准提出修正建议,以便质量标准更加科学客观,可操作性更强。 相似文献
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F P Gregorut L C Baptista A S Paulim J R Guerreiro E R Secato M Macari 《British poultry science》1992,33(4):769-774
1. The effect of bacterial endotoxin injection was studied in growing pullets of different ages. Commercial chicks were divided into 5 groups according to age. Bacterial endotoxins (E. coli and S. typhimurium) were injected intravenously and rectal temperature was measured over a period of 300 min. 2. The results showed no significant effect of age on the febrile response induced by bacterial endotoxins, but a slight tendency towards a reduced fever peak was observed with increasing age. The response latency also increased with age. 相似文献
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Data from the literature on the clinical effects of bacterial endotoxins in ruminants are reviewed. Special attention is paid to the effects on body temperature and reticulo-rumen motility. Furthermore, the effects of repeated intravenous injection of endotoxin are summarised. Pathophysiological disturbances after intramammary infusion of endotoxins proved to be identical to those found after intravenous injection of non-lethal doses. Strikingly, however, no marked inhibitory effect on rumen motility nor abortion was observed after intramammary infusion of endotoxins. Moreover, in cows that were made tolerant to endotoxin by daily intravenous injections, intramammary infusion of one-fifth of this daily dose produced a maximum effect on body temperature and plasma Zn concentrations. This suggests that inflammatory endogenous mediators were released in the udder and then absorbed into the blood circulation, rather than the absorption of endotoxin. 相似文献
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《Journal of aquatic animal health》2013,25(4):331-336
Abstract The efficacy of oxytetracycline was assessed as a potential therapy for male reproductive tract syndrome in a population of 160 previously affected, pond-reared, adult Penaeus vannamei. All animals were individually tagged, and the severity of disease was assessed grossly 2 weeks before, during, and 4 weeks after feed medicated with oxytetracycline hydrochloride was administered. Bacterial cultures indicated that more Vibrio sp. were isolated from animals with melanized spermatophores than from animals with normal spermatophores at the same site. Vibrio alginolyticus was the most common bacterial isolate, and the bacterial isolates were sensitive to oxytetracycline. A t-test analysis of the mean lesion severity scores showed no significant difference between the control animals and animals that received the medicated feed. A significant difference in severity scores among designated severity groups (low, medium, high; P < 0.01) was maintained throughout the experiment. Overall, the trend in all severity groups was a gradual increase in severity over time. These results suggest that this case of male reproductive tract syndrome was not responsive to oxytetracycline therapy. In addition, these results provide further evidence of a noninfectious primary etiology. 相似文献
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In vitro sensitivities to antimicrobial drugs of ureaplasmas isolated from the bovine respiratory tract, genital tract and eye 总被引：4，自引：0，他引：4
The sensitivity to 18 antimicrobial drugs was examined for 66 strains of Ureaplasma sp isolated from respiratory tracts of calves suffering from enzootic pneumonia, urinary tracts of bulls and eyes of cows suffering from infectious bovine kerato-conjunctivitis. Furamizole, tiamulin fumarate, erythromycin lactobionate, malidomycin C, doxycycline hydrochloride, kitasamycin tartrate, tylosin tartrate, T-2636C, tetracycline hydrochloride, oxytetracycline hydrochloride, chlortetracycline hydrochloride, oleandomycin phosphate, furazolidone, spiramycin adipate, chloramphenicol and thiophenicol showed strong inhibiting activity on all the test strains. Among them, furamizole, tiamulin fumarate and erythromycin lactobionate were most active. Kanamycin sulphate showed weak activity on all the strains tested. The differences in origin of the test strains did not affect their sensitivity to any of the drugs. 相似文献
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采用紫外分光光度法和高效液相色谱法测定盐酸土霉素可溶性粉中土霉素的含量。紫外分光光度法试验表明,用0.01 mol/L盐酸溶液作参比液,以268 nm为测定波长,土霉素在4~32μg/mL范围内吸光度与浓度呈良好的线性关系,相关系数r=0.9999,平均回收率(n=5)为101.04%,RSD=1.02%。高效液相色谱法试验表明,以0.05 mol/L草酸铵溶液-二甲基甲酰胺-0.2 mol/L磷酸氢二铵溶液(75∶20∶5)为流动相,以280 nm为检测波长,土霉素在0.02~0.14mg/mL范围内浓度与峰面积线性关系良好,相关系数r=0.9999,平均回收率(n=5)为100.52%,RSD=0.25%。紫外分光光度法、高效液相色谱法与抗生素微生物检定法比较,结果基本一致。 相似文献
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任含柳路美玉段鹏黄月辉 《中国兽药杂志》2020,54(3):49-52
为考察新型长效土霉素注射液的安全性,分别对小白鼠和家兔进行急性毒性和局部刺激性试验。随机将60只小白鼠分为6组,每组10只,其中雌雄各半,进行急性毒性试验考察;随机将9只家兔分为3组,每组3只,进行局部刺激性试验。结果表明,该新型长效土霉素注射液小鼠肌肉注射LD 50为600.32 mg/kg,LD 50的95%的可信区间为(600.32±2.44)mg/kg;随着时间延长和给药剂量增加,刺激性增强,注射长效土霉素7 d后,家兔股四头肌出现红肿、发紫、坏死、光泽消失现象,15 d后肌肉组织的坏死范围扩大。该产品对小白鼠表现为低毒,对家兔的股四头肌有局部刺激性。 相似文献