共查询到20条相似文献，搜索用时 65 毫秒
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本研究利用组织培养技术对进行培养挽救,以为了提高育种成功率.试验结果表明:外植体的最佳消毒方法为75％酒精消毒30 s后在0.1％升汞处理8min,幼胚的成活率为90％.随着外植体消毒时间增加,幼胚成活率先上升后下降.幼胚最佳萌发培养基为1/2MS+ BA0.1 mg·L-1+ NAA0.01 mg·L-1.最佳继代增殖培养基为MS+ BA0.1 mg· L-1+ NAA0.01 mg·L-1,增殖系数达到6.33.对不定芽增殖最有利条件为:pH值6.0、琼脂浓度6g·L-1和蔗糖浓度30 g·L-1.最佳生根培养基为1/2MS+ IBA0.2 mg·L-1,生根率为93.2％. 相似文献
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杂交马褂木组织培养技术研究 总被引：1，自引：1，他引：0
外植体采自杂交马褂木的幼树或扦插苗,采集部位为其顶芽或腋芽,最佳培养基为MS+BA2.0 mg/L+NAA0.1 mg/L。采用DCR为基本培养基,加入抗褐化试剂柠檬酸50.0mg/L,Vc 50.0 mg/L,继代增殖率可以达到2.8倍,最高达到4倍。生根培养基为1/2MS+NAA1.5 mg/L+活性炭0.5 g/L,瓶苗生根率高达61%。组培苗直接移栽到红心土,成活率达到93%以上。 相似文献
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以野生耐盐变异柽柳植株的萌动芽为材料,进行了不同植物生长调节剂及浓度对萌动芽生长、生长芽分化、不定芽生根的影响及试管苗的移栽、扦插、移植试验,成功建立了耐盐野生变异柽柳植株的无性系。结果表明:MS+BA 0.1 mg.L-1+GA 0.5 mg.L-1+IAA 0.2 mg.L-1是暗培养条件下培养芽生长的理想培养基;MS+AgNO31.0 mg.L-1+BA 1.0mg.L-1+NAA 0.1 mg.L-1是不定芽分化培养的理想培养基;1/2MS+IAA 0.4 mg.L-1是试管苗生根培养的理想培养基;移植到海边沙滩上的试管苗保持了耐盐变异性状,并且生长旺盛,根系发达。 相似文献
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以叶片、叶柄或叶茎为外植体,在培养基MS+6-BA 1～2mg/L+NAA 0.1mg/L+2,4-D 0.1～0.2mg/L上均可诱导愈伤组织的产生,其中以MS+6-BA1.0 mg/L+NAA0.1mg/L+2,4-D 0.1mg/L效果最好,诱导率可达87%以上;其最佳的分化培养基MS+6-BA 1.5mg/L+NAA 0.2mg/L上,分化率可以达到90%以上,大哥大红掌最佳的增殖培养MS+6-BA 2mg/L+NAA0.2mg/L,培养30d芽的增殖系数达到3.45,降低6-BA浓度或提高NAA浓度有利于培养壮苗;1/2MS+NAA 0.1mg/L+1g/L活性炭的培养基诱导植株生根效果最好,生根率86%,平均每株苗生根3条;生根试管苗移栽于泥炭土:珍珠岩=5∶1的基质,成活率约86%。 相似文献
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为了探索白花泡桐的幼化技术,以13个白花泡桐优树为试验材料,通过组织培养法对白花泡桐优树材料的幼化技术进行了研究。结果表明:嫁接嫩芽为最适合的外植体;MS+BA 4.0 mg.L-1+NAA 0.3mg.L-1为初代芽诱导最佳培养基;1/2 MS+BA4.0 mg.L-1+NAA0.3 mg.L-1为继代培养幼化的最合适的培养基;12个白花泡桐优树材料成功得到幼化。1/2 MS+NAA0.1 mg.L-1或1/2 MS+IBA0.1 mg.L-1+NAA0.1 mg.L-1为最理想的生根培养基。炼苗在室内进行,炼苗一个月后大棚壮苗,成活率可达95%以上。 相似文献
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《森林与环境学报》2010,(1)
以紫毛野牡丹茎段为外植体,以MS为基本培养基,通过不同的激素种类和浓度配比,对紫毛野牡丹组培快繁体系建立进行了研究。结果表明,外植体表面消毒用70%乙醇浸泡30s,无菌水冲洗2遍,0.1%升汞浸泡5-7min,无菌水冲洗5遍消毒效果最好,污染率仅为21%;最佳初代培养基配方是MS+6-BA1.0mg.L-1+NAA0.5mg.L-1;继代培养最佳配方为MS+6-BA1.0mg.L-1+NAA0.1mg.L-1,平均增殖率达到3.4;最佳生根配方为1/2MS+NAA(IBA)0.1-0.2mg.L-1;生根苗移栽于黄心土以及混合基质中,成活率达到80%。 相似文献
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金叶风箱果组培技术研究! 总被引：1，自引：0，他引：1
通过对金叶风箱果组培工厂化育苗技术研究的阐述,总结了金叶风箱果组培工厂化育苗最优培养基配方:诱导培养基配方为MS+BA 0.6mg.L-1+NAA 0.05mg.L-1+蔗糖35g.L-1+琼脂8g.L-1,诱导率达到了84%;增殖培养基配方为MS+BA 1.2mg.L-1+IBA 1.0mg.L-1+蔗糖35g.L-1+琼脂8g.L-1,增殖倍数达到6.11;生根培养基配方为1/2MS+NAA0.05mg.L-1+IBA 1.0mg.L-1+蔗糖35g.L-1+琼脂8g.L-1,生根率达95%。 相似文献
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摘要：外植体采自杂交马褂木的幼树或扦插苗,采集部位为其顶芽或腋芽,最佳培养基为MS+BA 2.0 mg/L +NAA 0.1 mg/L。采用DCR为基本培养基,加入抗褐化试剂柠檬酸50.0 mg/L,Vc 50.0 mg/L,继代增殖率可以达到2.8倍,最高达到4倍。生根培养基为1/2MS+NAA 1.5 mg/L +活性炭0.5 g/L,瓶苗生根率高达61%。组培苗直接移栽到红心土,成活率达到93%以上。 相似文献
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《Scandinavian Journal of Forest Research》2012,27(5):427-435
In Scandinavia, moose (Alces alces L.) sometimes cause severe browsing damage to economically-important pine. Moose-vehicle accidents have spurred construction of fences along roads, and these may interfere with moose migration between summer and winter ranges, or the road alone may be a barrier. If this happens and moose build up along roads, landowners may suffer economically. Therefore, this study investigated whether roads, fences or other factors influence the use of young pine stands by moose. Eighty stands along roads in northern Sweden were evaluated in which individually-browsed branches were counted on 9972 pines. Moose browsing was not significantly related to birch (Betula pendula Roth, B. pubescens Ehrh.) density, nor did it differ between pines (Pinus contorta Douglas or P. sylvestris L.). However, increased pine density, site productivity and proximity to a highway were associated with increased browsing. Further large-scale studies are needed to understand moose habitat selection and the effects of roads. 相似文献
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《Scandinavian Journal of Forest Research》2012,27(3):206-217
The root systems of 2-yr-old Picea glauca, Picea mariana and Pinus banksiana seedlings were submitted to various frost temperatures during an artificial frost to induce different levels of root damage. Frost-damaged and control seedlings were placed in a greenhouse under high and low soil moisture regimes. Seedling growth and physiology were evaluated periodically. Seedling survival was reduced when root damage reached levels of 60-80%. Root systems of all three species showed partial to total recovery by the end of the experiment. In general, root freezing damage caused reductions in seedling growth, with these reductions becoming less significant over time. Root damage had little to no effect on black spruce and jack pine seedling physiology, while white spruce CO 2 uptake decreased with increasing root damage. Shoot nitrogen content of all three species decreased slightly with increasing root damage. 相似文献
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Contour hedgerows of multipurpose tree species in the sloping tea lands of Sri Lanka are expected to reduce soil erosion and
also add significant amounts of plant nutrients to the soil via periodic prunings. The objective of this experiment was to characterize the biomass decomposition pattern and quantify the
amount of nutrients added through prunings of six tree species (Calliandra calothyrsus, Senna spectabilis, Euphatorium innulifolium, Flemingia congesta, Gliricidia sepium and Tithonia diversifolia) currently being used in hedgerows associated with tea. Withered leaf and stem prunings (50 g) were enclosed in 2-mm litter
bags, placed at 5-cm depth and retrieved after one, three, six, nine and 12 weeks. Loss of initial dry weight, N, P and K
was measured. Single exponential decay function adequately described both dry weight and nutrient loss. Tree species differed
significantly in their rate of breakdown with decomposition constants (k) varying from 0.0299 to 0.2006 week−1 for leaves and from 0.0225 to 0.0633 week−1 for stems. Gliricidia showed the highest k for leaves with the rest in the following descending order: Senna > Tithonia ≥ Euphatorium > Calliandra > Flemingia. A similar pattern was observed for loss of all nutrients with Calliandra and Flemingia always having lower k values than the rest. Although N immobilization was not observed, immobilization of P and K was observed
during the first week of incubation in some species, particularly in stem prunings. Annual biomass of prunings differed significantly
between tree species in the following descending order: Calliandra > Senna > Flemingia > Tithonia > Gliricidia > Euphatorium. Calliandra added the greatest amount of nutrients annually to the soil with Euphatorium adding the least. Calliandra prunings provided the annual total K requirement and 49% of the N requirement of mature tea. However, none of the species
provided more than 5% of the P requirement. It is concluded that among the tree species tested, Calliandra and Flemingia are the most suitable for contour hedgerows in tea plantations of this agroclimatic region because of their higher soil nutrient
enrichment capacity and slower decomposition rates which would minimize leaching losses.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
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Astra Zaluma Lauma Bruna Darta Klavina Natalija Burnevica Kristine Kenigsvalde Andis Lazdins Talis Gaitnieks 《Forest Pathology》2019,49(6)
Heterobasidion parviporum and Heterobasidion annosum are widely distributed root‐rot fungi that infect conifers throughout Europe. Infection of conifer stumps by spores of these pathogens can be controlled by treating fresh stumps with a competing non‐pathogenic fungus, Phlebiopsis gigantea. In this study, growth of three Latvian strains of P. gigantea and the biological control agent ‘Rotstop’ strain was evaluated in stem pieces of Norway spruce, Scots pine, lodgepole pine, Douglas‐fir, Weymouth pine, Siberian larch and Sitka spruce. The growth rates of one H. parviporum and one H. annosum isolate were also measured in the same stem pieces. The growth rate of P. gigantea varied greatly in wood of different conifer species. It was higher in the three pine species, lower in Norway spruce and lowest in Sitka spruce and Siberian larch, and in Douglas‐fir, this fungus did not grow. The largest area of wood occupied by P. gigantea was in lodgepole pine. Growth of Latvian isolates of P. gigantea in the wood of Pinus and Picea species was comparable to that of the Rotstop isolate. Consequently, stump treatment with local P. gigantea isolates should be recommended. However, our results suggest that Douglas‐fir stump treatment against Heterobasidion by P. gigantea may be ineffective and other stump treatment methods should be considered. 相似文献
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《Scandinavian Journal of Forest Research》2012,27(2):118-130
The effects of seed weight and seed type on seedling growth of Pinus sylvestris (L.) were studied by seeding individually weighed orchard and stand seed in different mixtures under harsh (direct seeding in field) and optimal (seeding in nursery) conditions. In the nursery experiment an increase in the seed weight from 3 to 7 mg increased the seedling height by 10-27% and total weight by 27-113%, and decreased the height/diameter ratio by 5-6% after 2 yrs. With elimination of seed weight effects, orchard seedlings were 2% taller than stand seedlings in year 2. Without elimination of seed weight effects, orchard seedlings were 7-13% taller. In the field experiment an increase in the seed weight from 3 to 7 mg increased seedling height by 18-65%, stem volume by 81-274% and the number of top-buds by 23-34% in year 5. After elimination of seed weight effects, orchard seedlings were 7-13% taller than stand seedlings and without elimination of seed weight effects 20-21% taller after 5 yrs. Even after elimination of both seed weight and genetic effects orchard seedlings were 3-9% larger than stand seedlings in the field experiment. In conclusion, the influence of seed weight and seed type on growth traits and slenderness is highly significant and the influence seems to be greater in harsh conditions. 相似文献
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《Scandinavian Journal of Forest Research》2012,27(1):53-61
This study investigated the stand structure in pine, spruce and deciduous forests in the border district of Finland and Russia. A total of 46 mature forest stands was selected as pairs, the members of each pair being as similar as possible with respect to their forest site type, age, moisture and topography. The stands were then compared between the two countries by means of basal areas and number of stems. The proportions of dominating tree species were 2-12% lower, and correspondingly the proportions of secondary tree species higher, in Russian forests. The density of the forest stock was also higher in each forest type in Russia. The forests in the two countries differed most radically in terms of the abundance of dead trees. The amount was two to four times higher in Russian deciduous and spruce forests, and in pine forests the difference was 10-fold. The stand structures indicated that Russian coniferous stands, in particular, were more heterogeneous than intensively managed pine and spruce stands in Finland. 相似文献
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《Scandinavian Journal of Forest Research》2012,27(1):15-27
The root collar diameter and the height:diameter ratio are of particular importance in container-grown seedlings where a high density in the containers may produce spindly seedlings. Temperature regimes and light quality are known to affect plant growth. The aim of this study was to identify responses in Picea abies (L.) Karst. seedlings grown with light providing different red:far-red ratios and under temperature regimes with alternating day (DT) and night temperature (NT) from negative (DT < NT) to positive (DT > NT) difference (DIF) between DT and NT. Experiments were conducted in controlled environment chambers and in a daylight phytotron. Only limited thermoperiodic responses appeared in P . abies seedlings with respect to seedling height and dry weight accumulation. The formation of terminal buds, however, was clearly delayed in seedlings grown at negative DIF. The results indicate a requirement for day extension light that is high in far-red, to prevent terminal bud formation under natural short-day conditions. An extended study should be conducted to clarify the minimum level of light intensity and the optimal light quality needed to prevent terminal bud formation under natural short-day conditions. 相似文献
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本文分析了CAD在设计中引起的正面和负面影响,并进行了系统的阐述,从而使设计者在应用中能够保持客观的态度。 相似文献
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Steven Maranz Amadou Niang Antoine Kalinganire Djeneba Konaté Bocary Kaya 《Agroforestry Systems》2008,72(3):231-239
Baobab leaves form an important part of the local diet in Sahel countries and elsewhere in Africa. Existing leaf nutritional
data and agroforestry performance information are based solely on Adansonia digitata L., the baobab of continental Africa. The introduction potential of Adansonia species from the center of diversity in Madagascar and from Australia remains untapped. To assess this potential, the mineral
contents and B1 and B2 vitamin levels of dried baobab leaves were determined for five-year old trees of A. digitata, A. gibbosa (A. Cunn.) Guymer ex D. Baum, A. rubrostipa Jum. & H. Perrier (syn. A. fony Baill.), A. perrieri Capuron and A. za Baill. grown in an introduction trial in Mali. Nutritional data were evaluated against survival and vigor to identify promising
germplasm. Leaf vitamin and crude protein contents were highest in the Madagascar species, especially A. rubrostipa (B1 88 mg 100 g−1, B2 187 mg 100 g−1, protein 20.7% dry weight). However, the local species far outperformed the introductions in survival, tree height, basal
diameter and resistance to termites. We suggest grafting as a way of harnessing the vigor of well-adapted local baobab varieties
to the superior nutritional profiles of A. rubrostipa and others. Cross-species grafting tests in Adansonia were successful, thus creating new agroforestry possibilities with different scion/rootstock combinations. 相似文献
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《Wood material science & engineering》2013,8(2):76-84
Abstract Wood in general and wooden studs in particular are often distorted owing to uneven shrinkage during the drying process in the sawmill. Twist is often the most detrimental of all types of distortion, and it is caused by spiral grain in combination with variations in moisture content. For sawmills, the objective is to produce dried, straight boards, and one method of dealing with boards with excessive spiral grain is to sort them out and then dry them in a pretwisted position to obtain straight boards after drying. A model using the finite element (FE) method for the simulation of drying twist distortions was first calibrated against laboratory experiments in which boards were dried with and without restraints and pretwists. After the calibration, the FE results were compared with industrial test results for boards that were dried without restraints or with restraints with zero pretwist, i.e. straight restraints. The FE model used an elastic–ideally plastic material model to obtain permanent deformations. The calibration was to set the yield stresses so that there was a good match between FE results and results from the laboratory experiments. The comparison between the industrial test results and the FE results showed that the FE model is capable of realistic simulations of drying boards with and without restraints and presumably also pretwists. 相似文献