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R. P. Bansal R. C. Joshi B. Sharma S. K. Bandyopadhyay U. Chandra 《Tropical animal health and production》1986,18(3):133-136
Summary The single radial hemolysis [SRH] test was employed for detection of rinderpest antibodies in post-vaccinated serum samples
as also in serum samples from animals recovered from rinderpest infection. The results were compared with counterimmunoelectrophoresis
[CIE] and serum neutralisation [SN] tests. The CIE test was found to be more sensitive than SRH but because of ease and simplicity
SRH can also be used for monitoring antibody development after vaccination.
Resumen Se utilizó la prueba radial sencilla de hemólisis (RSH) para detectar anticuerpos de rinderpest, en muestras de suero de animales vacunados y recuperados de la enfermedad. Los resultados se compararon con las pruebas de contrainmunoelectroforésis (CIE) y sero neutralización (SN). La prueba CIE fue más sensitiva que la RSH, pero debido a la sencillez de manejo, se recomienda la RSH para medir el nivel de anticuerpos post vacunales.
Résumé Le test d’hémolyse radiale simple (HRS) a été utilisé pour la détection des anticorps antibovipestiques dans des échantillons de sérums après vaccination et aussi dans des échantillons de sérums d’animaux convalescents. Les résultats ont été comparés avec les tests de contre-immunoélectrophorèse (CIE) et de séroneutralisation (SN). On a trouvé que le test CIE est plus sensible que le HRS mais par suite de son aisance et de sa facilité, le HRS peut aussi être employé pour suivre le développement des anticorps après vaccination.相似文献
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R. P. Bansal R. C. Joshi B. Sharma U. Chandra 《Tropical animal health and production》1987,19(1):53-55
Reverse phase passive haemagglutination [RPHA] test was applied for the detection of rinderpest antigen in various organs of rinderpest infected cattle. The results of RPHA were compared with counter immunoelectrophoresis [CIE] and single radial haemolysis [SRH] test. RPHA was as sensitive as CIE and SRH in detecting rinderpest antigen. 相似文献
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Fixed parameters for different hypothetical strains of rinderpest virus (RV) and different susceptible populations are described together with details of their derivation. Simulations were then carried out in a computer model to determine the effects that varying these parameters would have on the behaviour of RV in the different populations. The results indicated that virulent strains of RV are more likely to behave in epidemic fashion whereas milder strains tend towards persistence and the establishment of endemicity. High herd immunity levels prevent virus transmission and low herd immunity levels encourage epidemic transmission. Intermediate levels of immunity assist the establishment of endemicity. The virus is able to persist in large populations for longer than in small populations. Different vaccination strategies were also investigated. In areas where vaccination is inefficient annual vaccination of all stock may be the best policy for inducing high levels of herd immunity. In endemic areas and in herds recovering from epidemics the prevalence of clinically affected animals may be very low. In these situations veterinary officers are more likely to find clinical cases by examining cattle for mouth lesions rather than by checking for diarrhoea or high mortalities. 相似文献
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Summary A microplate enzyme-linked immunosorbent assay (ELISA) was developed which detected antibodies to a soluble antigen prepared from sonicated rinderpest virus-infected cells. The ELISA detected titres of antibody to the virus in the sera of cattle 3 weeks after immunisation with tissue culture rinderpest virus vaccine which were similar to those detected by the virus neutralisation test. The ELISA test shows potential as a rapid and economic technique for screening large numbers of sera for antibody to rinderpest virus.
La Prueba Micro-Elisa Para Detectar Anticuerpos Producidos Por Antigenos Del Virus De Rinderpest
Resumen Se utilizó la prueba micro-ELISA para detectar anticuerpos producidos por un antígeno soluble preparado con células sonicadasinfectadas con el virus de rinderpest. La prueba ELISA detectó anticuerpos en el suero de bovinos, 3 semanas después de que éstos fueron inmunizados con la vacuna de rinderpest, preparada ésta en cultivos celulares. Los anticuerpos detectados fueron similares a los estudiados mediante la prueba de neutralización viral. La prueba ELISA se perfila como una técnica rápida y económica para trabajar un número apreciable de muestras de suero con el fin de detectar anticuerpos del virus de rinderpest.
Un Micro-Test Elisa Pour Deceler Les Anticorps Specifiques Du Vir Us Bovipestique
Résumé Un test immuno-enzymatique (ELISA) a été mis au point déceler les anticorps correspondant à un antigène préparé par traitement aux ultra-sons de cellules infectées. Les titres sériques obtenus par cette méthode dans les sérums de bovins immunisés trois semaines auparavant avec du vaccin de culture cellulaire se sont révélés comparables à ceux obtenus par la méthode classique de séroneutralisation. Le test ELISA apparait comme un moyen rapide et économique pour rechercher les anticorps spécifiques du virus bovipestique dans des sérums en grand nombre.相似文献
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The development of an epidemiological model of rinderpest in cattle and wildlife populations is described. The model uses a state-transition structure, incorporating a stochastic element through the use of Monte-Carlo methods modified to allow large populations to be simulated. The potential applications include the estimation of "safe" host population immunity rates and the design of cost-effective rinderpest vaccination programmes. 相似文献
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R. C. Joshi B. Sharma S. K. Bandyopadhyay R. P. Bansal 《Tropical animal health and production》1984,16(3):167-170
Rinderpest antibodies were detected by employing the fluorescent antibody test (FAT) and the immunoperoxidase test (IPT) and the results were compared with the counterimmuno electrophoresis test (CIE). FAT was found to be the most sensitive in detecting post-vaccinal antibodies followed by IPT and CIE tests. 相似文献
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N. D. J. Chandran K. Kumanan R. A. Venkatesan 《Tropical animal health and production》1995,27(2):89-92
Summary Six field isolates believed to be rinderpest viruses and 2 known strains of peste des petits ruminants (PPR) viruses were titrated in the presence of normal rabbit serum and with hyperimmune rinderpest antiserum prepared in rabbits. The known PPR viruses had indices less than 10 whereas 4 of the suspect field isolates had indices greater than a hundred. Two suspect field isolates had indices less than 20; both were collected from small ruminant wild life and are probably PPR viruses.
Diferenciacion De Peste De Los Pequenos Rumiantes Y Virus De Rinderpest Mediante Indices De Neutrilizacion Utilizando Antisueros Hiperinmunes De Rinderpest
Resumen Seis aislamientos de campo sospediosos de Rinderpest y dos cepas conocidas de Peste de los pequeños rumiantes (PPR) fueron titulados en titulados en presencia de suero normal de conejo y suero hiperimmune a Rinderpest, prepardo en conjo. Las cepas concidos de PPR dierond titulos menores de 10, mientras que 4 de los aislamientos sospechosos de Rinderpest dieron titulos mayores de 100. Los 2 aislamientos de campo restantes dieron titulos menores de 20. Ambos fueron aislados de pequenños ruminantes silvestras y probablemente sean virus de PPR.
Differenciation Des Virus De La Peste Bovine Et De La Peste Des Petits Ruminants Par Les Indices De Neutralisation Utilisant Des Antiserums Hyperimmuns De La Peste Bovine
Résumé La titration de 6 isolats recuellis sur le terrain et considérés comme étant du virus de la peste bovine, et 2 souches connues du virus de la peste des petits ruminants (PPR) ont été titrés en présence de sérum normal de lapin et avec un antisérum hyperimmun de la peste bovine préparé sur lapins. Les virus identifiés PPR avaient des indices inférieurs à 10 alors que 4 des isolats suspects présentaient des indices supérieurs à 100. Deux isolats suspects avaient des indices inférieurs à 20 et tous deux provenaient de petits ruminants sauvages et sont probablement des virus de la PPR.相似文献
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The direct immunoperoxidase test has been used to detect rinderpest virus antigens in infected bovine kidney cell cultures to study the multiplication of the virus. Infected bovine kidney coverslip cultures were sequentially tested with peroxidase-labelled, anti-rinderpest globulins at 3, 6, 24, 48, 72, 96, 120 and 144 h post-infection. The progressive virus-specific cytopathic changes compared well with the increase in the number of cells showing the presence of viral antigens when tested by the direct immunoperoxidase test. The specificity of the reaction was confirmed by using negative and antiserum-blocked BK cell cultures on coverslips. 相似文献
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