共查询到19条相似文献，搜索用时 187 毫秒
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《四川畜牧兽医》2021,(9)
为探讨制作小脊椎动物骨骼透明标本的适宜环境温度和碱液浓度,缩短骨骼透明标本的制作时间,更好地观察动物体整体骨骼的形态以及骨骼与肌肉的位置关系,本试验分别在三种不同的自然气温条件下选择四种小脊椎动物(蛙、鲫鱼、仔鼠和金鱼)致死后除去内脏,经过固定、脱水脱脂、软化透明、染色、脱色、固定保存等步骤制作骨骼透明标本;另选择在中等温度和室温条件下采用不同碱液浓度对标本进行软化透明。试验结果显示:不同的室温条件对标本制作的影响明显,气温越高完成标本的制作周期越短,染色效果也能达到预期的效果;碱液浓度越高对标本软组织的软化透明越快,但碱液浓度要逐步升高,而且不同体积的标本要选用不同的碱液浓度。 相似文献
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一种心脏透明标本的制作方法 总被引：1，自引：0，他引：1
为了进行心脏透明标本制作方法的研究,完整暴露心脏的冠状循环,便于学生直观地观察心脏自身的血液循环,笔者在实践中不断摸索,成功地制作了一种心脏透明标本,现报道如下. 相似文献
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为了制作效果良好的肝片形吸虫制片标本,以便于更好的为教学和科研服务,对福尔马林固定、苏木精染色、二甲苯透明及中性树胶封固等处理方法的用量和处理时间进行摸索,寻找最佳条件,提高肝片形吸虫制片标本的效果.结果表明对标本进行酒精梯度脱水处理并延长二甲苯的透明时间,制片标本的观察效果明显提高,经处理后的虫体染色效果良好,吸盘、消化道、生殖系统等特征结构清晰可见. 相似文献
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寄生虫标本在寄生虫学的教学、科研、寄生虫病的诊断和防治工作中发挥着十分重要的作用.将寄生虫制成玻片标本,可延长标本的保存时间,且可显示原始状态下难以观察的结构,是鉴别寄生虫虫种的重要手段和方法.一个永久性虫体标本的制作过程包括固定、染色、脱水、透明和封片等五大步骤,但具体到不同虫种,实际操作步骤亦有所不同. 相似文献
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主要介绍了动物病理大体标本和病理组织切片的制作过程,包括取材、处理、固定、保存、透明、浸蜡、包埋及切片染色等,同时介绍了动物病理组织标本在兽医病理学理论教学及实践或实验教学过程中的重要作用,在提高学生的学习兴趣、培养学生的综合应用能力方面具有重要意义。 相似文献
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展示骨骼系统的透明标本已成为研究骨骼形态发育的重要方法,被广泛应用于比较解剖 学领域,尤其适用于难以剖解的胚胎和小型脊椎动物.它不仅是研究骨骼和关节在体内的 自然位置和连接形式的重要方法,还是研究动物生长发育期骨骼的定型和骨化进程,以及胚 胎毒理学等领域的独特方法.小鼠是哺乳动物的模式动物[1],在胚后初期的生长 发育速度非常快,是研究骨骼生长的重要阶段[2].本文利用透明标本的制作技术 ,对断奶前小鼠四肢骨骼的形态学变化进行了观察,对骨骼的形成和发育情况进行了探讨. 相似文献
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Rafael Latorre Kees de Jong Mircea‐Constantin Sora Octavio Lpez‐Albors Carlos Baptista 《Anatomia, histologia, embryologia》2019,48(6):557-563
Epoxy plastination techniques were developed to obtain thin transparent body slices with high anatomical detail. This is facilitated because the plastinated tissue is transparent and the topography of the anatomical structures well preserved. For this reason, thin epoxy slices are currently used for research purposes in both macroscopic and microscopic studies. The protocol for the conventional epoxy technique (E12) follows the main steps of plastination—specimen preparation, dehydration, impregnation and curing/casting. Preparation begins with selection of the specimen, followed by freezing and slicing. Either fresh or fixed (embalmed) tissue is suitable for epoxy plastination, while slice thickness is kept between 1.5 and 3 mm. Impregnation mixture is made of epoxy E12 resin plus E1 hardener (100 ppw; 28 ppw). This mixture is reactive and temperature sensitive, and for this reason, total impregnation time under vacuum at room laboratory temperature should not last for more than 20–24 hr. Casting of impregnated slices is done in either flat chambers or by the so‐called sandwich method in either fresh mixture or the one used for impregnation. Curing is completed at 40°C to allow a complete polymerization of the epoxy‐mixture. After curing, slices can be photographed, scanned or used for anatomical study under screen negatoscope, magnification glass or fluorescent microscope. Based on epoxy sheet plastination, many anatomical papers have recent observations of and/or clarification of anatomical concepts in different areas of medical expertice. 相似文献
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An intermediate-technology pattern matching model and decision support system for veterinary diagnosis is described. Six diseases of cattle occurring in the tropics are used to illustrate the model. The pattern matching model is composed of a series of transparent overlays and a template. Each transparent overlay represents a sign state and contains sign frequency information for the diseases on the template. By superimposing multiple transparent overlays upon the disease template, a ranked list of differential diagnoses can be obtained. Ranking is by summation of disease sign frequencies. Modifications to accommodate observational uncertainty are presented. Disease prevalences can be represented in the model. 相似文献
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在“三黄连”合剂质量标准基础上,通过光加速试验、加速试验考查温度、湿度、光照等因素对该制剂理化性质的影响,评价“三黄连”合剂的稳定性,为新兽药申报、生产、包装、贮存和运输条件提供必要资料。采用薄层色谱法对“三黄连”合剂中的有效成分黄芩苷、连翘苷进行定性鉴别,结合pH值、相对密度、有效成分含量等检测指标,进行光加速试验和加速试验。结果表明,“三黄连”合剂在规定时间检测,在各自对照品相应位置上均显相同颜色的斑点;pH值、相对密度、有效成分含量等均符合规定;“三黄连”合剂对光不敏感,在生产、贮存和运输过程中不必考虑避光,可以采用透明容器进行包装。 相似文献
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Nguyen Viet LINH Kazuhiro KIKUCHI Michiko NAKAI Fuminori TANIHARA Junko NOGUCHI Hiroyuki KANEKO Thanh Quang DANG-NGUYEN Nguyen Thi MEN Nguyen VAN HANH Tamas SOMFAI Bui Xuan NGUYEN Takashi NAGAI Noboru MANABE 《The Journal of reproduction and development》2013,59(6):549-556
Mitochondria are reported to be critical in in vitro maturation of
oocytes and subsequent embryo development after fertilization, but their contribution
for fertilization has not been investigated in detail. In the present study, we
investigate the contribution of mitochondria to fertilization using reconstructed
porcine oocytes by fusion of ooplasmic fragments produced by serial centrifugations
(centri-fusion). Firstly, we evaluated the characteristics of ooplasmic fragments.
Three types of fragments were obtained by centrifugation of porcine oocytes matured
in vitro for 46 h: brownish (B), transparent (T) and large (L)
fragments containing both B and T parts in a fragment. The production efficiencies of
these types of fragments were 71.7, 91.0 and 17.8 fragments/100 oocytes,
respectively. In experiments, L fragments were excluded because they contained both
brownish and transparent components that were apparently intermediate between B and T
fragments. Observations by confocal microscopy after staining with MitoTracker Red
CMXRos® and transmission electron microscopy revealed highly condensed
active mitochondria in B fragments in contrast to T fragments that contained only
sparse organelles. We reconstructed oocytes by fusion of a karyoplast and two
cytoplasts from B and T fragments (B and T oocytes, respectively). The B oocytes
showed higher sperm penetration (95.8%) and male pronuclear formation rates (94.2%)
by in vitro fertilization than T oocytes (66.7% and 50.0%,
respectively). These results suggest that the active mitochondria in oocytes may be
related to their ability for fertilization. 相似文献