共查询到19条相似文献，搜索用时 657 毫秒
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试验以"临川虎奶菇-0121"为供试菌株,对虎奶菇"冷菌热菇"种植模式进行探究。结果表明,在低温季节发菌,可利用虎奶菇自身的呼吸作用产热提升温度,能促使菌丝体生长加快,提前完成发菌。采用带盖套环封口技术,在阻隔杂菌的同时,空气流通好,也更有利于虎奶菇菌丝体生长。 相似文献
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虎奶菇生物学特性研究 总被引：4，自引：3，他引：1
研究了影响好氧菌虎奶菇菌核生长发育的因子。结果表明,虎奶菇菌丝生长最适温度为３２℃,菌核生长适温为２３～２８℃;增养料消毒前较适宜ｐＨ为７．５～８;适宜料水比为１∶２．２;较适宜碳源为玉米淀粉和淀粉;较适宜氮源为麦麸和牛粪。 相似文献
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矮生观赏杉木DNA甲基化的水平与模式分析 总被引：1，自引：0，他引：1
为探讨杉木矮化变异与DNA甲基化的关系,以矮生观赏杉木与野生杉木为试验材料,采用基于DNA甲基化敏感扩增多态性分析（Methylation-sensitive amplification polymorphism,MSAP）方法,研究其DNA 序列CCGG 位点的甲基化水平及模式变化特征。应用20个引物组合,在矮生观赏杉木和野生杉木的叶片DNA中均检测出745个CCGG位点,其中甲基化位点数分别为508个和505个,分别占总扩增位点数的68.17%和67.83%;在矮生观赏杉木与野生杉木木质部DNA中分别检测到742个和737个CCGG位点,其中甲基化位点数分别为471个与498个,分别占总扩增位点数的63.52%和67.51%,差异达极显著水平（P < 0.01）。与野生型相比,矮生观赏杉木叶片和木质部DNA甲基化模式发生了一定变化,在叶片DNA中,去甲基化率为17.81%,明显高于超甲基化率15.44%;在木质部DNA中,去甲基化率17.25%,也明显高于超甲基化率14.65%。通过甲基化序列的初步克隆及比对分析发现,矮生观赏杉木中参与MAPK级联途径的蛋白磷酸酶IBR5基因启动子区域的甲基化水平上升。因此推测,植物激素信号转导及其调控基因的甲基化变化可能是矮生观赏杉木形成的原因之一。 相似文献
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AIM:To explore the DNA methylation markers for non-invasive prenatal diagnosis of trisomy 21. METHODS:The DNA methylation ratios of 3 genes (4 fragments) on chromosome 21 (CGI149, CGI045, HLCS-1 and HLCS-2) in blood cells from 13 non-pregnant women, and in placental tissues from 15 euploid and 11 trisomy 21 pregnant women were measured using the methods of methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA) and bisulfite sequencing in combination. RESULTS:The results obtained from MS-MLPA were consistent with the results from bisulfite sequencing. The fragments of CGI149, CGI045 and HLCS-2 were unmethylated in the non-pregnant woman blood cells. HLCS-1 and HLCS-2 were methylated in all euploid and all trisomy 21 placentae. CGI149 was methylated in 13 (13/15) of the euploid and 10 (10/11) of the trisomy 21 placental tissues. CGI045 was methylated in 11 (11/15) of the euploid and all the Trisomy 21 placental tissues. Only HLCS-2 was found to be methylated in all placental tissues but unmethylated in all non-pregnant woman blood cells. Only the DNA methylation ratio in CGI149 was significantly different between euploid and trisomy 21 placental tissue (P<0.05). No difference among HLCS-1, HLCS-2 and CGI045 was observed. CONCLUSION: MS-MLPA is an effective alternative to bisulfite sequencing for the assessment of methylation ratios in placental tissue. CGI149, CGI045, HLCS-1 and HLCS-2 are not appropriate DNA methylation markers for non-invasive prenatal diagnosis of trisomy 21. 相似文献
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AIM：To examine DNA methylation at CpG sites in the promoter region of tumor necrosis factor-alpha (TNF-α) gene in dengue virus type 2 (DENV2)-infected peripheral blood mononuclear cells (PBMC).
METHODS：DNA methylation in the promoter region of TNF-α gene was measured by bisulfite sequencing PCR.
RESULTS：The promoter region of TNF-α gene was from -294 bp to +58 bp, including 11 CpG sites. The PCR products identified by aga-rose gel electrophoresis were consistent with the theoretical size. Two sites were methylated at 0 h and 6 h and 6 sites were methylated at 12 h in TNF-α gene promoter region in DENV2-infected PBMC. The average methylation rates were 103%, 121% and 255% at 0 h, 6 h and 12 h, respectively. Significant differences between 0 h and 12 h and between 6 h and 12 h were observed.
CONCLUSION：The DNA methylation in the promoter region of TNF-α gene is increased in DENV2-infected PBMCs. 相似文献
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今年是《园艺学报》创刊发行50周年。50年来,《园艺学报》坚持为学术交流服务,为促进学科发展作贡献的办刊原则,以"科学性;创新性;对生产和科研有参考启迪作用"的标准,收录和发表了大量高水平的论文,记载了几代科技工作者呕心沥血创新之作,反映了中国园艺科学技术和园艺产业的发展历程。 相似文献
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AIM: To investigate the correlation of gene modification between histone H3 lysine 4 (H3K4) trimethylation and DNA methylation in IgA nephropathy patients. METHODS: H3K4 trimethylation variations were analyzed in peripheral blood mononuclear cells (PBMCs) from 40 IgAN patients and 40 healthy controls by the method of chromatin immunoprecipitation linked to microarrays (ChIP-chip). ChIP real-time PCR was used to validate the microarray results. Quantitative real-time PCR (qRT-PCR) was employed to analyze mRNA expression. DNA methylation in 4 selected positive genes was analyzed by the method of methylated DNA immunoprecipitation-quantitative PCR (MeDIP-qPCR). RESULTS: IgAN patients displayed higher H3K4 trimethylation level and lower DNA methylation level than those of the healthy controls. There were significant differences in DNA methylation and H3K4 trimethylation of 4 selected genes between IgAN patients and the healthy controls (P<0.05). CONCLUSION: Our studies indicate that significant alterations of H3K4 trimethylation and DNA methylation exist in IgAN patients. There is a correlation of gene modification between DNA mathylation and histone H3 lysine 4 trimethylation in IgAN patients. 相似文献
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蜜环菌原种品种及配方筛选试验 总被引：1，自引：0，他引：1
比较了蜜环菌原种不同品种及配方的菌丝(索)生长速度,结果表明蜜环菌AM6、密A9、宁强A9-1三个菌株的菌丝(索)生长速度最快,分别为2.86 mm.d-1、2.80 mm.d-1、2.71 mm.d-1,与其他品种相比差异极显著;原种配方以配方2(木屑50%、玉米芯25%、麦麸12%、玉米粉10%、葡萄糖1%、石膏1%、石灰0.5%、磷酸二氢钾0.3%、硫酸镁0.2%、VB1 10 mg)最好,平均生长速度达2.18 mm.d-1,与其他配方差异极显著。因此,该研究筛选出的优良品种及配方可以在生产上推广应用。 相似文献
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LUO Hai-dan WANG Dong-hui LIN Wei-da SU Bo-jin ZHAO Cun-you YANG Hui-ling 《园艺学报》2015,31(7):1300-1304
AIM: To compare the differences of the genome-wide methylation levels and methylated regions between nasopharyngeal carcinoma (NPC) cells in the same genetic background but different radiation resistance (CNE-2 cells and CNE-2R cells). METHODS: Using the method which was developed by Doctor Zhao Cun-you, based on using methyl-sensitive restriction enzyme to measure the genome-wide methylation levels. In addition, MeDIP-Seq was used to analyze the methylated regions in 6 gene functional elements, including the upstream 2k sequence, 5'UTR, coding sequence, intron, 3'UTR and downstream 2k sequence, between CNE-2 cells and CNE-2R cells. RESULTS: The genome-wide methylation level was approximately 30% lower in CNE-2R cells than that in CNE-2 cells. No obvious difference on the amount of genes and the coverage of the peak in the 6 gene functional elements was observed. However, the methylation pattern of plentiful genes had altered in the gene function elements. CONCLUSION: The genome-wide methylation levels and methylated regions between NPC cells in the same genetic background but different radiation resistance were quite different, indicating that the DNA methylation may be associated with NPC radioresistance. 相似文献
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由丝核菌引起的甜瓜果腐病是辽宁省近年发生的新病害,在棚室甜瓜生产中发生渐趋严重。根据病原菌形态、致病性、菌丝融合群和rDNA-ITS序列分析,确定其病原菌为茄丝核菌（Rhizoctonia solani）,AG-4 HGI融合群。病原菌生物学特性测定结果表明,菌丝生长最适温度28 ℃;最适pH 6.0 ~ 8.0;供试10种培养基中,在甜瓜煎汁培养基中菌丝生长最快;供试9种碳源和10种氮源中,最适碳源为可溶性淀粉,最适氮源为蛋白胨;光照对菌丝生长影响较小。菌核形成最适条件为温度25 ℃,pH 7.0,理查培养基,黑暗条件;以山梨醇为碳源的PA培养基和以KNO3为氮源的查氏培养基有利于菌核形成。对于病菌菌核萌发胡萝卜煎汁培养基最为适宜;供试9种碳源和10种氮源中,最适碳源为麦芽糖,最适氮源为蛋白胨;菌核萌发最适温度28 ℃,最适pH 5.0 ~ 8.0,黑暗条件利于菌核萌发;菌丝致死温度50 ℃、10 min;菌核致死温度53 ℃、10 min。 相似文献