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1.
通过禽流感灭活抗原配合黏膜免疫佐剂鼻腔免疫乳鸽,研究鸽呼吸道各段抗体分泌细胞的分布和数量。结果显示,首免后第3、5周,应用CpG免疫后肺IgG分泌细胞面积显著高于对照组（P〈0.05）,首免后第5,7周,应用CpG免疫后肺IgA分泌细胞面积显著高于对照组（P〈0.05）;首免后第3、5、7周,应用灭活禽流感抗原免疫后,鸽呼吸道各部位IgG分泌细胞和IgA分泌细胞面积与对照组无显著差异;应用禽流感抗原配合CpG和胆酸钠免疫后鸽呼吸道各部位单位面积中IgA分泌细胞和IgG分泌细胞面积均显著或极显著高于对照组（P〈0.01,P〈0.05）。结果表明,灭活禽流感抗原配合黏膜免疫佐剂通过鼻腔免疫能够提高呼吸道中IgA分泌细胞和IgG分泌细胞的面积,增强局部呼吸道体液免疫应答水平。 相似文献
2.
《畜牧兽医学报》2016,(6)
拟研究左旋咪唑、聚乙烯亚胺和聚肌胞分别配合灭活H5N1禽流感病毒口服免疫雏鸡,空肠、回肠中IgA和IgG抗体分泌细胞数量和分布的变化。应用左旋咪唑、聚乙烯亚胺和聚肌胞分别配合灭活H5N1禽流感病毒经口免疫雏鸡后,通过免疫组织化学方法显示空肠、回肠IgA和IgG分泌细胞。聚乙烯亚胺配合灭活禽流感病毒口服免疫后,空肠、回肠IgA和IgG分泌细胞的数量均显著(P0.05)高于单独口服灭活禽流感病毒免疫后的数量;左旋咪唑和聚肌胞分别配合灭活禽流感病毒口服免疫后,空肠、回肠IgA分泌细胞数量显著(P0.05)增加,IgG分泌细胞数量无明显变化。研究表明,左旋咪唑、聚肌胞和聚乙烯亚胺分别配合灭活禽流感病毒口服免疫雏鸡,均能提高肠道局部体液免疫水平,聚乙烯亚胺的效果最好,价格更便宜。 相似文献
3.
口蹄疫病毒(foot-and-mouth disease virus,FMDV)主要通过呼吸道传播,切断呼吸道传播途径能有效的防御口蹄疫。本研究应用O型口蹄疫灭活病毒配合枯草芽胞杆菌通过喷鼻气雾免疫牛来探讨对牛呼吸道黏膜免疫应答及系统免疫应答的影响。结果显示,免疫后牛鼻黏膜与肺内支气管黏膜中的IgA分泌细胞的数量极显著增多(P0.01),而常规口蹄疫灭活苗对IgA分泌细胞数量的影响不大(P0.05);同时,ELISA结果显示鼻、气管、肺和肺内支气管中IL-12、TNF-α水平均极显著增加(P0.01),尤其是在气管和肺内支气管中最为明显,但IL-6无明显差异(P0.05)。从免疫第3天开始牛鼻液、唾液中口蹄疫特异性抗体SIgA以及血清中O型口蹄疫病毒特异性抗体均显著增加(P0.01或P0.05),且维持至3个月。本研究结果表明O型口蹄疫灭活病毒配合枯草芽胞杆菌喷鼻后可刺激牛呼吸道局部体液免疫和细胞免疫,同时还可诱导全身系统免疫应答,本研究为口蹄疫灭活病毒鼻腔免疫提供理论基础和应用前景。 相似文献
4.
猪呼吸道IgA和IgG分泌细胞的分布 总被引：1，自引：0，他引：1
旨在研究猪呼吸道IgA和IgG分泌细胞的分布。取健康50日龄猪的软腭扁桃体、咽扁桃体、气管和肺组织,应用免疫组织化学技术研究猪呼吸道中IgA和IgG分泌细胞的变化。结果显示,在软腭扁桃体中,IgA和IgG分泌细胞主要分布在隐窝上皮下;在咽扁桃体中,IgA和IgG分泌细胞主要集中分布在黏膜上皮下;在气管中,IgA和IgG分泌细胞主要分布在黏膜上皮下固有层和气管腺周围,在气管上皮之间也有少量IgA分泌细胞分布;肺中的IgA和IgG分泌细胞主要集中分布在肺泡隔和各级支气管的黏膜上皮下。另外,我们观察到肺中的2种分泌细胞着色明显比气管中淡。对2种分泌细胞进行统计学分析发现,IgA分泌细胞在气管中分布最多,其次是咽扁桃体、软腭扁桃体,肺中最少;而IgG分泌细胞则在咽扁桃体中最多,其次是气管、肺,软腭扁桃体中最少。结果表明,猪呼吸道含有较多的抗体分泌细胞,但不同部位不同的抗体分泌细胞分布不尽相同,该结果可为猪呼吸道黏膜免疫及相关疫病发生机理的研究提供理论依据。 相似文献
5.
《畜牧与兽医》2016,(3):117-120
研究左旋咪唑配合灭活H5N1禽流感病毒(IAIV)口服免疫对鸡免疫反应和生长性能的影响。用左旋咪唑配合灭活H5N1禽流感病毒口服免疫雏鸡后,通过检测口腔、肠道和呼吸道中特异性Ig A、血清中特异性Ig G和HI抗体水平对免疫效果进行评价,通过称重、检测空肠绒毛长度来评价生长性能。左旋咪唑配合灭活禽流感病毒口服免疫雏鸡后,口腔棉拭子、肠道和气管涮洗液中特异性Ig A水平极显著(P0.01)或显著(P0.05)高于单独口服IAIV;左旋咪唑配合灭活禽流感病毒口服免疫雏鸡后,体重没有变化。本研究表明,左旋咪唑配合灭活H5N1禽流感病毒口服免疫雏鸡,不仅能刺激局部的黏膜免疫应答,还能诱导全身免疫应答反应,对鸡的生长性能影响不大。 相似文献
6.
《中国预防兽医学报》2018,(10)
本研究采用O型口蹄疫灭活病毒配合枯草芽孢杆菌喷鼻气雾免疫健康牛,并设单独使用枯草芽孢杆菌组、灭活病毒、常规疫苗免疫组和空白对照组,评价O型口蹄疫灭活病毒配合免疫增强剂枯草芽孢杆菌通过喷鼻气雾免疫健康牛对其呼吸道先天免疫力的影响。通过免疫组织化学染色显示,免疫后灭活病毒配合免疫增强剂试验组牛鼻黏膜与肺内支气管黏膜中TLR7的表达和CD3~+淋巴细胞呈极显著增多(p0.01);而常规口蹄疫灭活苗对其TLR7的表达和CD3~+淋巴细胞数量的影响不显著(p0.05)。ELISA检测结果显示,与对照组相比,灭活病毒配合免疫增强剂试验组牛呼吸道组织中IL-12水平呈极显著增加(p0.01),IL-4和IL-10水平无明显差异(p0.05);而常规口蹄疫灭活苗对牛呼吸道组织中IL-4、IL-10和IL-12的影响不明显(p0.05)。表明O型口蹄疫灭活病毒配合枯草芽孢杆菌能够提高牛呼吸道先天免疫力。本研究为研发口蹄疫灭活病毒的鼻腔疫苗提供重要的试验数据和参考。 相似文献
7.
为了解H7N9亚型禽流感油乳灭活苗对不同品种鸭诱导免疫应答的基本能力和不同接种方法诱导各种鸭产生HI抗体的情况,试验采用H7N9亚型禽流感油乳灭活苗对4个品种(番鸭、白鸭、水鸭、麻鸭)的雏鸭进行3种不同程序的免疫试验(2周龄肌肉注射0.3 mL/只为程序1,2周龄肌肉注射0.5 mL/只为程序2,2周龄肌肉注射0.3 mL/只、3周龄肌肉注射0.6 mL/只为程序3)。结果表明,程序1免疫的雏鸭可在免疫后2周产生4.09~5.73 log_2HI抗体,免疫后1~8周均值为4.54~7.03 log_2;程序2免疫的雏鸭可在免疫后2周产生4.20~6.27 log_2的HI抗体,免疫后1~8周均值5.53~6.57 log_2;程序3免疫的雏鸭可在免后2周达到4.91~7.36 log_2,免疫后1~8周均值6.31~7.87 log_2。结果提示,该疫苗对4种鸭都具有良好的免疫原性,免疫后可较快地产生较高水平的抗体,且维持较长时间(8周以上),同时初步建立了鸭H7N9亚型禽流感油乳灭活疫苗的基本免疫程序。 相似文献
8.
几种黏膜免疫佐剂对鸡小肠IgA分泌细胞的影响 总被引：8，自引：2，他引：8
分别在新城疫Ⅳ系弱毒苗中添加黏膜免疫佐剂乳酸杆菌、CpG DNA、重组IL-2、氟化钠和大豆黄酮，经口免疫鸡后，研究十二指肠、空肠、Peyer’s斑单位面积IgA分泌细胞的变化。首先提纯鸡IgA和制备兔抗鸡IgA血清，然后应用免疫组化技术显示鸡小肠IgA分泌细胞。结果表明，在免疫后第3周、第5周乳酸杆菌组比新城疫组（ND）极显著增加各段小肠IgA分泌细胞的数量（P〈0．01）；CpGDNA、重组IL-2和大豆黄酮在整个免疫期内均明显增加鸡小肠黏膜局部IgA分泌细胞数量；NaF对鸡体黏膜局部IgA分泌细胞数量无明显增加。结果表明乳酸杆菌、CpGDNA、重组IL-2和大豆黄酮是有效的口服黏膜免疫佐剂。 相似文献
9.
10.
为探讨鸭瘟弱毒苗诱导鸭局部黏膜和系统免疫中抗体发生的规律,将鸭瘟病毒Cha株弱毒苗皮下注射免疫20日龄樱桃谷鸭后60 d内定时随机剖杀5只鸭,采集血清、胆汁、气管和消化道(食道、十二指肠、空肠、回肠、盲肠和直肠)分泌液,应用间接ELISA检测抗DPV的IgA、IgM和IgG滴度(以Log10表示).结果表明:①血清:抗体滴度由高到低为IgG、IgM和IgA,相应的检测到的时间段分别为免疫后6～60,3～15,12～36 d.②胆汁:抗体滴度由高到低为IgA、IgG和IgM,相应的检测到的时间段分别为免疫后9～21,15～27,3～12 d.③分泌液:气管和消化道分泌液中抗体滴度由高到低均为IgA、IgM和IgG,其中IgA抗体滴度由高到低为十二指肠、食道、气管、空肠、盲肠、回肠和直肠,相应的检测到IgA的时间段分别为免疫后3～60,9～60,3～60,9～60,12～60,12～27,6～36d;IgM由高到低为气管、食道、十二指肠、空肠、盲肠、直肠和回肠,相应的检测到IgM的时间段分别为免疫后3～12,6～15,3～12,6～12,9～12,6～9,6～9 d;IgG由高到低为食道、十二指肠、气管、空肠、盲肠、直肠和回肠,相应的检测到IgG的时间段分别为免疫后9～36,12～27,6～36,9～36,12～36,9～21,15～21 d.综上,鸭瘟弱毒疫苗皮下免疫鸭后,IgM和IgG分别是系统免疫中体液免疫的先锋抗体和主要抗体;IgA是气管、消化道和胆汁中的主要抗体. 相似文献
11.
分泌IgA和IgG的浆细胞是黏膜免疫系统中两种重要的效应细胞。本研究旨在探究分泌IgA和IgG的浆细胞在家兔鼻腔中的分布特征。以20只健康成年家兔为研究对象，根据硬腭褶皱和牙齿特征，依次将家兔鼻腔分为Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅴ 5段，运用组织学、免疫组织化学、图像分析及统计学方法，对分泌IgA和IgG的浆细胞在家兔鼻腔中的分布特征进行了详细的研究。免疫组化结果显示：分泌IgA和IgG的浆细胞在家兔鼻腔每段都有分布，主要弥散分布于鼻黏膜固有层中；其形态呈圆形或卵圆形，核一般位于细胞一侧，胞质丰富呈阳性反应。统计结果显示：分泌IgA和IgG的浆细胞在鼻腔不同段的分布密度由高到低依次是：鼻腔第Ⅳ段、第Ⅴ段、第Ⅲ段、第Ⅱ段、第Ⅰ段。其中分泌IgA的浆细胞在鼻腔Ⅲ、Ⅳ、Ⅴ段的分布密度显著高于鼻腔Ⅰ、Ⅱ段（P<0.05），在上颌鼻甲、鼻中隔和筛鼻甲的分布密度显著高于上鼻甲（P<0.05）；与分泌IgA的浆细胞相比，分泌IgG的浆细胞除鼻腔第Ⅳ段的分布密度显著高于鼻腔Ⅲ、Ⅴ段（P<0.05）外，其分布变化趋势基本与分泌IgA的浆细胞类似。结果证明，分泌IgA和IgG的浆细胞弥散分布于家兔每段鼻腔的鼻黏膜固有层中，有利于SIgA和IgG分子在整个鼻黏膜形成完整的保护屏障；分泌IgA和IgG的浆细胞主要分布在家兔鼻腔Ⅲ、Ⅳ、Ⅴ段及上颌鼻甲、筛鼻甲和鼻中隔，提示这些区域是家兔鼻腔免疫的重要效应部位。本研究为家兔呼吸系统疾病的预防及进一步探讨家兔鼻腔免疫应答的机制提供重要的理论依据。 相似文献
12.
Pengcheng LI Yunfeng LI Guoqing SHAO Qinghua YU Qian YANG 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(5):519-525
The aim of this study was to evaluate the immune responses to intranasal and
intrapulmonary vaccinations with the attenuated Mycoplasma hyopneumoniae
(Mhp) 168 strain in the local respiratory tract in pigs. Twenty-four
pigs were randomly divided into 4 groups: an intranasal immunization group, an
intrapulmonary immunization group, an intramuscular immunization group and a control
group. The levels of local respiratory tract cellular and humoral immune responses were
investigated. The levels of interleukin (IL)-6 in the early stage of immunization
(P<0.01), local specific secretory IgA (sIgA) in nasal swab samples
(P<0.01); and IgA- and IgG-secreting cells in the nasal mucosa and
trachea were higher after intranasal vaccination (P<0.01) than in the
control group. Interestingly, intrapulmonary immunization induced much stronger immune
responses than intranasal immunization. Intrapulmonary immunization also significantly
increased the secretion of IL-6 and local specific sIgA and the numbers of IgA- and
IgG-secreting cells. The levels of IL-10 and interferon-γ in the nasal swab samples and
the numbers of CD4+ and CD8+ T lymphocytes in the lung and hilar
lymph nodes were significantly increased by intrapulmonary immunization compared with
those in the control group (P<0.01). These data suggest that
intrapulmonary immunization with attenuated Mhp is effective in evoking
local cellular and humoral immune responses in the respiratory tract. Intrapulmonary
immunization with Mhp may be a promising route for defense against
Mhp in pigs. 相似文献
13.
Lavage techniques were used to obtain secretions from the nasal cavity, trachea and bronchi of conscious horses. The techniques, which utilised fibreoptic endoscopy for recovery of tracheal and bronchial secretions, were well tolerated by the horses. The recovery rates of the lavaged fluids were acceptable, but were lowest for bronchial secretions, and there was minimal contamination by blood. The fluids were analysed for IgG and IgM by single radial immunodiffusion, and for IgA and albumin by rocket immunoelectrophoresis. Relative to albumin there was significantly more IgA and IgM, and significantly less IgG, in the nasal cavity than the trachea. IgA and IgM levels were greatest in the nasal cavity and decreased progressively to the bronchi, whereas IgG levels showed the reverse trend. The immunoglobulin: albumin ratios of secretions taken from many levels of the tract were significantly higher than those of serum, suggesting local production of immunoglobulin in the respiratory tract. 相似文献
14.
Influenza is a common respiratory disease in pigs, and since swine influenza viruses are zoonotic pathogens, they also pose human health risks. Pigs infected with influenza virus mount an effective immune response and are protected from subsequent challenge, whereas the currently available, inactivated-virus vaccine does not consistently confer complete protection to challenge. To develop and evaluate new vaccination strategies, it is imperative to fully understand the immune responses that are associated with protection following natural infection. Therefore, we have evaluated the phenotype and kinetics of immune responses to primary and re-challenge infection with H1N1 swine influenza virus in the pig. Through the use of isotype-specific antibody secreting cell ELISPOT assays, interferon-gamma ELISPOT assays and isotype-specific ELISAs on serum, nasal wash and bronchoalveolar lavage samples, we defined the humoral and cellular immune responses, both locally in the respiratory tract and systemically, to this viral infection. Virus-specific serum IgG, IgA, and HI titers all peaked 2-3 weeks after primary infection and did not substantially increase after re-challenge. The predominant virus-specific isotype in serum was IgG. Pigs responded with virus-specific IgG and IgA in both the upper (nasal washes) and lower (bronchoalveolar lavages) airways; IgA was the predominant isotype in both sites. Despite the fact that the pigs were completely protected from re-challenge, the antibody titers in the nasal washes increased. Results of the antibody-secreting cell ELISPOT assays demonstrated that the numbers of both IgG and IgA secreting cells in the nasal mucosa were dramatically higher than in any other tissue examined. In contrast, IFN-gamma secreting cells were predominantly localized to the spleen and tracheobronchial lymph nodes. These data will be helpful in the future development and evaluation of novel vaccines. 相似文献
15.
R F Sheldrake 《Veterinary immunology and immunopathology》1989,21(2):177-186
Experiments were conducted to determine whether intraperitoneal (IP) immunization and subsequent intratracheal (IT) challenge were able to augment the specific antibody response in secretions of the porcine respiratory tract. Following IP immunization and two IT challenges within a period of 18 days the specific IgG and IgA antibody response was elevated in respiratory tract secretions and serum. While a portion of the anti-ovalbumin (OVA) IgG in respiratory tract secretions was generated locally, it would appear that the bulk of anti-OVA IgA was derived from serum. 相似文献
16.
R F Sheldrake 《Research in veterinary science》1990,48(1):47-52
Pigs which had been immunised intraperitoneally with ovalbumin were subsequently challenged intratracheally with ovalbumin at intervals over a 200 day period. Intratracheal challenge 74 and 77 days after intraperitoneal priming resulted in a significant antigen-specific IgA response in respiratory tract secretion (RTS) on day 81. After a further 91 days, similar intratracheal challenge resulted in a lesser antigen specific IgA response in RTS. These trends were examined relative to antigen-specific IgA levels in serum and relative to antigen-specific IgG levels in serum and RTS. In addition, at the completion of the experiment the majority of anti-ovalbumin containing cells (AOCC) in the tracheal lamina propria were of the IgA class. Previously it has been shown that intratracheal challenge alone with ovalbumin had a negligible effect on AOCC in the trachea, and on specific antibody levels in respiratory tract secretion. The present findings suggest the existence of a memory mechanism operating in the porcine respiratory tract. 相似文献
17.
Xuefeng Qi Xiaoyan Yang Anchun Cheng Mingshu Wang Dekang Zhu Renyong Jia Qihui Luo Xiaoyue Chen 《Research in veterinary science》2009,87(2):218-225
Duck virus enteritis (DVE) is an acute and contagious herpes virus infection of duck, geese and swans with high morbidity and mortality. The development of specific mucosal immune system against duck enteritis virus (DEV) infection for ducks has been hindered by a lack of knowledge concerning the purification of immunoglobulin A (IgA) of duck. In the present work, the method for purification of duck immunoglobulin A was developed, and the induction of intestinal mucosal immune responses against DEV was studied by orally infected ducklings with virulent DEV. The results showed that a continuous increased DEV DNA levels were observed in blood and various organs examined of orally infected ducklings throughout the infection, which was accompanied by the development of infection in ducklings from mild progressed to severe pathological lesions. Furthermore, a marked increased level of DEV-specific IgA and IgG antibodies in bile, serum and the intestinal tract, as well as the density of IgA+ cells in intestine were detected between 1 and 12 days p.i., followed by a drastic reduction of the antibody levels and the density of IgA+ cells at 15 days p.i. The results indicate that the DVE infection can stimulate both IgA-dominated antibody immune responses in the intestinal tract, and IgG-dominated antibody systemic immunity in the serum of ducklings orally inoculated with virulent DEV. The severe lesions of the villus epithelial cells and the lymphoid organs can suppress the intestinal mucosal immune responses. 相似文献
18.
感染鸡贫血病毒雏鸡接种新城疫疫苗后局部粘膜免疫功能的变化 总被引：6，自引：0，他引：6
雏鸡1日龄感染鸡贫血病毒（CAV），8日龄接种Lasota疫苗，以未感染免疫雏鸡为对照，于免疫后7、14、28d检测其哈德尔腺和盲肠扁桃体T细胞及IgG^ 、IgM^ 、IgA^ 抗体生成细胞数量，泪液、气管液、肠液、胆汁中IgG、IgM、IgA含量以及泪液、胆汁HI抗体滴度的动态变化。揭示了感染CAV雏鸡接种ND疫苗免疫后哈德尔腺、盲肠扁桃体的T细胞和IgG^ 、IgM^ 、IgA^ 抗体生成细胞数量，泪液、气管液、肠液、胆汁中免疫球蛋白IgG、IgM、IgA含量以及泪液、胆汁HI抗体滴度，均较未感染免疫雏鸡明显减少。表明眼部、呼吸道和消化道局部粘膜免疫防御能力减弱。 相似文献
19.
Gnotobiotic calves were inoculated by the intratracheal route with Mycoplasma bovis and the specific antibody response in sera and tracheo-bronchial washings examined by radioimmunoassay. In sera an IgM response which reached a peak two weeks post infection was followed by IgG1 and IgG2 antibody responses. Low levels of IgA antibody were detected in sera three and four weeks after infection. The predominant antibody in tracheo-bronchial washings 2 weeks after infection was IgA. Four weeks after infection IgG1 antibody predominated, but IgG2 and IgA antibodies were also present. Cells containing Ig were present in the cellular accumulations around the necrotic zones produced by M. bovis in the lung parenchyma two and four weeks after infection. IgG1 containing cells predominated in these cellular infiltrates. IgG2 producing cells were the next in frequency. It is concluded that the lung lesion caused by M. bovis is partly due to the host's immune response, presumably contributing to the control of the infection, and that the cells infiltrating the lung are a major source of the local and systemic IgG antibody that is detected after infection. IgA staining cells were observed in the submucosa of tissues from nasal cavity and trachea. These cells are probably the source of IgA in tracheo-bronchial washings and sera since IgA-producing cells were not a predominant component of the lesion in the lung parenchyma. 相似文献