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骨髓间充质干细胞的研究进展 总被引：1，自引：0，他引：1
骨髓间充质干细胞是存在于骨髓中的除造血干细胞以外的另一类具有多向分化潜能的干细胞。在一定的诱导条件下 ,这类细胞可定向分化为多种造血以外组织 ,特别是中胚层和神经外胚层来源的组织细胞。例如成骨细胞、成软骨细胞、脂肪细胞、腱细胞、肌肉细胞、神经细胞等。骨髓间充质干细胞具有贴壁生长的特性 ,在体外易分离和扩增 ,还易于外源基因的转入和表达 ,在人类医学上被认为是一种理想的治疗性细胞和基因治疗中的靶细胞。本文针对骨髓间充质干细胞的研究进展和在临床医学上的应用进行综述 相似文献
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《黑龙江畜牧兽医》2014,(11)
为了验证联合应用两种干细胞治疗肝硬化疾病的有效性,试验采用贴壁培养法分离小鼠骨髓间充质干细胞,并在体外单克隆纯化,采用免疫磁珠法分离脐带血造血干细胞,对造血干细胞进行无血清培养及生物学性状检测,然后用间充质干细胞联合造血干细胞附加肝细胞生长因子治疗Babl/c裸鼠肝硬化。结果表明:成功纯化培养小鼠骨髓间充质干细胞,体外无血清培养获得脐带血造血干细胞;肝细胞生长因子40 ng/mL、间充质干细胞5×106个、造血干细胞5×106个组作用于Babl/c裸鼠肝硬化模型,Babl/c裸鼠肝功能显著改善,丙氨酸转氨酶由198 U/L下降至86 U/L,天冬氨酸转氨酶由398.7 U/L下降至288.6 U/L。说明小鼠骨髓间充质干细胞能够在体外实现单克隆纯化,小鼠脐带血造血干细胞可以在体外无血清培养并大量克隆,干细胞对肝硬化疾病具有明显的治疗效果。 相似文献
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鸡的免疫球蛋白及其应用 总被引：1，自引：0，他引：1
梁荣 《国外兽医学(畜禽疾病)》1996,17(2):4-6
鸡的中枢免疫系统由骨髓、胸腺和法氏囊组成，来自骨髓的多能干细胞在胸腺被诱导分化为胸腺依赖性淋巴细胞（Ｔ细胞）参与细胞介导的免疫反应。来自骨髓的多能干细胞进入法氏囊成熟分化为囊依赖性淋巴细胞（Ｂ细胞），再运输到外周免疫器官，参与体液免疫反应。最近的研究证实法氏囊还有二级淋巴样器官的功能，即它能捕捉抗原和合成某些抗体。与哺乳动物ＩｇＧ相比，鸡的ＩｇＧ（ＩｇＹ）具有取材方便、产量高、稳定性好以及可消除用 相似文献
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旨在对山羊的骨髓间充质干细胞(BMSCs)进行分离培养。将分离得到的BMSCs进行传代培养,采用RT-PCR法检测其干细胞转录因子oct4和sox2基因,以验证其干细胞特性;在此基础上绘制BMSCs的生长曲线,对其生物学特性进行直观了解。结果表明,分离到的山羊BMSCs原代细胞呈现出成纤维样,并能够表达oct4和sox2基因,证明了其干细胞特性;其生长曲线呈"S"型,在1~2 d时为潜伏期,第3天时进入指数生长期,第7天时进入平台期。结果提示,分离得到的细胞具有BMSCs的特性,能够用于后续的相关试验研究。 相似文献
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鸡的免疫系统及其作用机理凌育（广东省家禽科学研究所广州市石井镇５１０４３０）鸡的免疫系统包括法氏囊、骨髓、脾脏、哈德氏腺、盲肠扁桃体和原始淋巴结（ｐｒｉｍｉｔｉｖｅｌｙｍｐｈｎｏｄｅｓ）。淋巴细胞是主要的免疫功能细胞，起始于淋巴干细胞，后者来源于孵化... 相似文献
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Generation of neuronal-like cells from umbilical cord blood-derived mesenchymal stem cells of a RFP-transgenic cloned cat 总被引：1，自引：0，他引：1
Jin GZ Yin XJ Yu XF Cho SJ Choi EG Lee YS Jeon JT Yee ST Kong IK 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(7):723-726
Umbilical cord blood (UCB)-derived mesenchymal stem cells (MSCs) are multipotent adult stem cells, which can differentiation into cells of connective tissue and neural lineages. This study investigated the potential for neuronal differentiation of red fluorescent protein (RFP)-transgenic cat UCB-derived MSCs. The cells were cultured in pre-induction medium for 24 hr and in neuronal-induction medium for 72 hr. Immunofluorescent staining showed that 6.85% of the total cells were beta III-tubulin-positive, 3.37% were neurofilament light (NF-L)-positive and 7.04% were neurofilament medium (NF-M)-positive. A beta III-tubulin band was detected by western blot analysis. Our results demonstrate that RFP-transgenic UCB-derived MSCs can be differentiated into neuronal cells in vitro. Thus, RFP-transgenic MSCs could provide alternative tracing material for stem cell transplantation. 相似文献
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Ferrari M Corradi A Lazzaretti M De'Cillà M Losi CG Villa R Lanfranchi A 《Veterinary research communications》2007,31(Z1):1-8
The use of adult stem cells in tissue regeneration appears to be a powerful research tool, due to the intrinsic characteristics of these cells, i.e., self-renewal and unlimited capacity for proliferation. In particular, mesenchymal stem cells (MSCs) obtained from bone marrow or peripheral blood can be easily isolated, cultivated, propagated and can be differentiated into several specialized cell types thanks to their plasticity. Among these cells, MSCs can evolve into cardiac cell lineages. Since heart damage leads to the irreversible loss of cardiac function, cell transplantation could be a potential therapy for heart injury. Our laboratory has focused on the purification and expansion of rat and sheep MSCs, their differentiation into cardiomyocytes and their characterisation. Numerous results indicate that MSCs could be promising for therapy, however we need to better understand the biology of stem cells to improve methods for delivery and/or pharmacological activation. These techniques can indeed track engrafted cells and systems to guarantee their safe use. 相似文献
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Arnhold SJ Goletz I Klein H Stumpf G Beluche LA Rohde C Addicks K Litzke LF 《American journal of veterinary research》2007,68(10):1095-1105
OBJECTIVE: To isolate and characterize bone marrow-derived equine mesenchymal stem cells (MSCs) for possible future therapeutic applications in horses. SAMPLE POPULATION: Equine MSCs were isolated from bone marrow aspirates obtained from the sternum of 30 donor horses. PROCEDURES: Cells were cultured in medium (alpha-minimum essential medium) with a fetal calf serum content of 20%. Equine MSC features were analyzed to determine selfrenewing and differentiation capacity. For potential therapeutic applications, the migratory potential of equine MSCs was determined. An adenoviral vector was used to determine the transduction rate of equine MSCs. RESULTS: Equine MSCs can be culture-expanded. Equine MSCs undergo cryopreservation in liquid nitrogen without altering morphologic characteristics. Furthermore, equine MSCs maintain their ability to proliferate and differentiate after thawing. Immunocytochemically, the expression of the stem cell marker CD90 can be detected on equine MSCs. The multilineage differentiation potential of equine MSCs was revealed by their ability to undergo adipogenic, osteogenic, and chondrogenic differentiation. CONCLUSIONS AND CLINICAL RELEVANCE: Our data indicate that bone marrow-derived stromal cells of horses can be characterized as MSCs. Equine MSCs have a high transduction rate and migratory potential and adapt to scaffold material in culture. As an autologous cell population, equine MSCs can be regarded as a promising cell population for tissue engineering in lesions of the musculoskeletal system in horses. 相似文献
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Impact of source tissue and ex vivo expansion on the characterization of goat mesenchymal stem cells
Nuradilla Mohamad-Fauzi Pablo J Ross Elizabeth A Maga James D Murray 《畜牧与生物技术杂志(英文版)》2015,(2):230-251
Background: There is considerable interest in using goats as models for genetically engineering dairy animals and also for using stem cells as therapeutics for bone and cartilage repair. Mesenchymal stem cells(MSCs) have been isolated and characterized from various species, but are poorly characterized in goats.Results: Goat MSCs isolated from bone marrow(BM-MSCs) and adipose tissue(ASCs) have the ability to undergo osteogenic, adipogenic and chondrogenic differentiation. Cytochemical staining and gene expression analysis show that ASCs have a greater capacity for adipogenic differentiation compared to BM-MSCs and fibroblasts. Different methods of inducing adipogenesis also affect the extent and profile of adipogenic differentiation in MSCs. Goat fibroblasts were not capable of osteogenesis, hence distinguishing them from the MSCs. Goat MSCs and fibroblasts express CD90, CD105, CD73 but not CD45, and exhibit cytoplasmic localization of OCT4 protein. Goat MSCs can be stably transfected by Nucleofection, but, as evidenced by colony-forming efficiency(CFE), yield significantly different levels of progenitor cells that are robust enough to proliferate into colonies of integrants following G418 selection.BM-MSCs expanded over increasing passages in vitro maintained karyotypic stability up to 20 passages in culture,exhibited an increase in adipogenic differentiation and CFE, but showed altered morphology and amenability to genetic modification by selection.Conclusions: Our findings provide characterization information on goat MSCs, and show that there can be significant differences between MSCs isolated from different tissues and from within the same tissue. Fibroblasts do not exhibit trilineage differentiation potential at the same capacity as MSCs, making it a more reliable method for distinguishing MSCs from fibroblasts, compared to cell surface marker expression. 相似文献
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Robert J. Harman 《Veterinary dermatology》2013,24(1):90-e24
Background – Adult stem cells come from many sources and have the capacity to differentiate into many cell types, including those of the skin. The most commonly studied stem cells are those termed mesenchymal stem cells (MSCs), which are easily isolated from bone marrow and adipose tissue. Mesenchymal stem cells are known to produce a wide array of cytokines that modulate the regeneration process. The ease of collection, propagation and use of these MSCs in therapy of traumatic, ischaemic and immune‐mediated skin conditions is emerging. Approach and evidence – In traumatic and ischaemic skin damage, MSCs are used in tissue‐engineered skin and by direct injection into damaged tissue. For immune‐mediated diseases, systemic administration of stem cells can modulate the immune system. The earliest clinical work has been with autologous stem cell sources, such as adipose tissue and bone marrow. In immune‐mediated diseases, the MSCs are used to downregulate production of inflammatory cytokines and to block T‐cell activation. Cells are generally given intravenously. Multiple sclerosis, rheumatoid arthritis and lupus have been successfully treated in human clinical trials. Mesenchymal stem cells can also stimulate resident local cells, such as keratinocytes and progenitor cells, to proliferate, migrate and repair skin injury and disease. Looking ahead – The discovery of the MSC in adipose tissue has spawned a global effort to utilize these cells in therapy of a wide range of diseases of the skin. Reconstructive surgery, scar blocking and resolution and skin regeneration have all been shown to be possible in human and animal studies. 相似文献
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《畜牧与生物技术杂志(英文版)》2016,(4)
Background: Adult mesenchymal stem cells(MSCs) can be conveniently sampled from bone marrow, peripheral blood, muscle, adipose and connective tissue, harvested from various species, including, rodents, dogs, cats, horses,sheep, goats and human beings. The MSCs isolated from adult tissues vary in their morphological and functional properties. These variations are further complicated when cells are expanded by passaging in culture. These differences and changes in MSCs must be considered prior to their application in the clinic or in a basic research study. Goats are commonly used as animal models for bone tissue engineering to test the potential of stem cells for bone regeneration. As a result, goat MSCs isolated from bone marrow or adipose tissue should be evaluated using in vitro assays, prior to their application in a tissue engineering project.Results: In this study, we compared the stem cell properties of MSCs isolated from goat bone marrow and adipose tissue. We used quantitative and qualitative assays with a focus on osteogenesis, including, colony forming unit, rate of cell proliferation, tri-lineage differentiation and expression profiling of key signal transduction proteins to compare MSCs from low and high passages. Primary cultures generated from each source displayed the stem cell characteristics,with variations in their osteogenic potentials. Most importantly, low passaged bone marrow MSCs displayed a significantly higher and superior osteogenic potential, and hence, will be the preferred choice for bone tissue engineering in future in vivo experiments. In the bone marrow MSCs, this process is potentially mediated by the p38 MAPK pathway. On the other hand, osteogenic differentiation in the adipose tissue MSCs may involve the p44/42 MAPK pathway.Conclusions: Based on these data, we can conclude that bone marrow and fat-derived MSCs undergo osteogenesis via two distinct signaling pathways. Even though the bone marrow MSCs are the preferred source for bone tissue engineering, the adipose tissue MSCs are an attractive alternative source and undergo osteo-differentiation differently from the bone marrow MSCs and hence, might require a cell-based enhancer/inducer to improve their osteogenic regenerative capacity. 相似文献
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Ki-Soo Park Yong-Soon Lee Kyung-Sun Kang 《Journal of veterinary science (Suw?n-si, Korea)》2006,7(4):343-348
Mesenchymal stem cells (MSCs) have the capabilities for self-renewal and differentiation into cells with the phenotypes of bone, cartilage, neurons and fat cells. These features of MSCs have attracted the attention of investigators for using MSCs for cell-based therapies to treat several human diseases. Because bone marrow-derived cells, which are a main source of MSCs, are not always acceptable due to a significant drop in their cell number and proliferative/differentiation capacity with age, human umbilical cord blood (UCB) cells are good substitutes for BMCs due to the immaturity of newborn cells. Although the isolation of hematopoietic stem cells from UCB has been well established, the isolation and characterization of MSCs from UCB still need to be established and evaluated. In this study, we isolated and characterized MSCs. UCB-derived mononuclear cells, which gave rise to adherent cells, exhibited either an osteoclast or a mesenchymal-like phenotype. The attached cells with mesenchymal phenotypes displayed fibroblast-like morphologies, and they expressed mesenchym-related antigens (SH2 and vimentin) and periodic acid Schiff activity. Also, UCB-derived MSCs were able to transdifferentiate into bone and 2 types of neuronal cells, in vitro. Therefore, it is suggested that the MSCs from UCB might be a good alternative to bone marrow cells for transplantation or cell therapy. 相似文献
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Min-Soo Seo Yun-Hyeok Jeong Jeung-Ran Park Sang-Bum Park Kyoung-Hwan Rho Hyung-Sik Kim Kyung-Rok Yu Seung-Hee Lee Ji-Won Jung Yong-Soon Lee Kyung-Sun Kang 《Journal of veterinary science (Suw?n-si, Korea)》2009,10(3):181-187
Human umbilical cord blood-derived mesenchymal stem cells (MSCs) are known to possess the potential for multiple differentiations abilities in vitro and in vivo. In canine system, studying stem cell therapy is important, but so far, stem cells from canine were not identified and characterized. In this study, we successfully isolated and characterized MSCs from the canine umbilical cord and its fetal blood. Canine MSCs (cMSCs) were grown in medium containing low glucose DMEM with 20% FBS. The cMSCs have stem cells expression patterns which are concerned with MSCs surface markers by fluorescence-activated cell sorter analysis. The cMSCs had multipotent abilities. In the neuronal differentiation study, the cMSCs expressed the neuronal markers glial fibrillary acidic protein (GFAP), neuronal class III β tubulin (Tuj-1), neurofilament M (NF160) in the basal culture media. After neuronal differentiation, the cMSCs expressed the neuronal markers Nestin, GFAP, Tuj-1, microtubule-associated protein 2, NF160. In the osteogenic & chondrogenic differentiation studies, cMSCs were stained with alizarin red and toluidine blue staining, respectively. With osteogenic differentiation, the cMSCs presented osteoblastic differentiation genes by RT-PCR. This finding also suggests that cMSCs might have the ability to differentiate multipotentially. It was concluded that isolated MSCs from canine cord blood have multipotential differentiation abilities. Therefore, it is suggested that cMSCs may represent a be a good model system for stem cell biology and could be useful as a therapeutic modality for canine incurable or intractable diseases, including spinal cord injuries in future regenerative medicine studies. 相似文献
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Reich CM Raabe O Wenisch S Bridger PS Kramer M Arnhold S 《Veterinary research communications》2012,36(2):139-148
In the dog, mesenchymal stem cells (MSCs) have been shown to reside in the bone marrow (bone marrow-derived mesenchymal stem cells: BM-MSCs) as well as in the adipose tissue (adipose tissue-derived stem cells: ADSCs). Potential application fields for these multipotent MSCs in small animal practice are joint diseases as MSCs of both sources have shown to possess chondrogenic differentiation ability. However, it is not clear whether the chondrogenic differentiation potential of cells of these two distinct tissues is truly equal. Therefore, we compared MSCs of both origins in this study in terms of their chondrogenic differentiation ability and suitability for clinical application. BM-MSCs harvested from the femoral neck and ADSCs from intra-abdominal fat tissue were examined for their morphology, population doubling time (PDT) and CD90 surface antigen expression. RT-PCR served to assess expression of pluripotency marker Oct4 and early differentiation marker genes. Chondrogenic differentiation ability was compared and validated using histochemistry, transmission electron microscopy (TEM) and quantitative RT-PCR. Both cell populations presented a highly similar morphology and marker expression in an undifferentiated stage except that freshly isolated ADSCs demonstrated a significantly faster PDT than BM-MSCs. In contrast, BM-MSCs revealed a morphological superior cartilage formation by the production of a more abundant and structured hyaline matrix and higher expression of lineage specific genes under the applied standard differentiation protocol. However, further investigations are necessary in order to find out if chondrogenic differentiation can be improved in canine ADSCs using different protocols and/or supplements. 相似文献