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草莓种质资源常低温保存试验 总被引：2，自引：0，他引：2
大多数草莓不耐高温 ,在南方常常难以越夏 ,这就给南方草莓种质资源的田间保存带来很大难度。每年我所草莓资源圃均有草莓植株因夏季高温而死亡。植物离体保存技术是植物种质资源保存的又一种方法 ,由于不受外界环境条件的影响 ,因而具有田间保存无法比拟的优点。植物离体保存方法一般可分为超低温保存、常低温保存和常温保存。超低温保存一般所需费用较大 ,适用于长期保存。常温保存一般使用植物生长抑制剂 ,保存时间较长 ,保存结束后植株恢复生长较慢。常低温保存所需费用较低 ,保存结束后植株恢复生长较快 ,恢复生长技术较简单。本试验针… 相似文献
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以3种食用百合试管苗为试材,采用限制生长保存法,研究了不同浓度生长抑制剂对食用百合试管苗保存效果的影响,并对保存后的试管苗进行遗传稳定性检测,以期建立食用百合种质资源离体保存体系。结果表明:龙芽百合试管苗用10mg·L~(-1)防落素(PCPA)处理保存300d后,试管苗生长缓慢,存活率达96.00%;川百合试管苗用2.0mg·L~(-1)青鲜素(MH)处理保存150d后,试管苗的抑制生长效果明显,结鳞率和存活率高达100.00%;兰州百合试管苗用10mg·L~(-1)多效唑(PP333)处理保存300d后,试管苗抑制作用明显,结鳞率达100.00%,存活率达94.40%。比较限制生长保存后与未保存植株的可溶性蛋白和酯酶同工酶图谱,各处理和对照图谱带相似,初步证明了以上保存方法的可行性,较好的保持了遗传稳定性。 相似文献
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百合种质资源限制生长法保存研究 总被引：12，自引：0，他引：12
对切花百合(Lilium L. ) 品种‘Siberia’ (西伯利亚) 试管苗限制生长保存方法及保存后恢复生长的再生植株的遗传稳定性进行了研究。结果表明, 常温( 20 ±1) ℃、光照强度1 500 lx、光照时间12 h /d的条件下, 在MS或者1 /2MS培养基中添加1.0～3.0 mg/L的脱落酸(ABA) 能够有效延长百合试管苗的继代间隔时间。离体保存百合试管苗9个月时存活率均在80%以上。在添加50～90 g/L高浓度蔗糖的培养基中能够保存百合试管苗11个月以上, 保存过程中植株生长缓慢, 基部有紫色鳞茎生成。而添加10～50 g/L甘露醇或10～40 mg/L矮壮素(CCC) 对百合试管苗生长没有起到明显的限制作用。各种限制生长法保存的试管苗转移到恢复培养基上培养1个月左右均能长成正常的植株, 其形态特征以及可溶性蛋白质、过氧化物酶同工酶和酯酶同工酶图谱与对照相比没有明显差异。 相似文献
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园林植物养护存在的问题及解决对策 总被引：1，自引：0，他引：1
针对目前我国园林植物养护环节薄弱的现状,从园林植物养护的重要性出发,对园林植物养护中存在的问题进行分析,并提出了相应的解决方法。 相似文献
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以寒地城市文化休闲广场—哈尔滨市开发区景观广场为例,从植物种类、植物数量、植物规格、种植形式及养护投入角度,对广场的可持续植物景观营造所存在的一些问题进行了分析。提出了寒地城市休闲文化广场可持续植物景观营造的基本策略:铺设生态型透水铺装,收集利用道路与广场雨水;收集利用山坡、微地形和其它绿地雨水;合理规划植物种植种类、数量、规格与形式;科学设置绿荫广场,创造舒适的林下休闲活动空间。 相似文献
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Although urban habitats contribute to the conservation of species diversity, urbanization has significantly reduced biodiversity by causing environmental changes such as habitat loss and fragmentation. Therefore, research on urban biodiversity studies has become increasingly important. Historical heritage sites are recognized as important habitats in remnant green spaces in urban areas. We aimed to evaluate the role of historical sites in conserving biodiversity in urban areas. As the land in these historical sites is not modified, they have the potential to conserve biodiversity through continued maintenance activities such as mowing and tree cutting. In Japan, Tamagawa-josui, a waterway that runs from west to east through the Tokyo megacity (35° 40′ N, 139° 25′ E), has been recognized as a civil engineering heritage landmark that preserves water utilization technology from the early modern period (1600s, Edo-era). The present study examined the relationships between plant diversity and green space in a historic site of a megacity (i.e., Tamagawa-josui) and determined the factors that influence plant diversity. Specifically, we examined the relationships between plant species indices (species richness and species compositions) and environmental factors (management, environmental conditions, and landscape factors). The present study analyzed spatial changes in the plant species composition in Tamagawa-josui. We demonstrated that tree canopy openness was positively correlated with plant species richness, and the increased disturbance associated with developing historical sites as urban parks was negatively correlated with native plant species richness. In addition, there was significant species turnover in the plant community from upstream to downstream in Tamagawa-josui, which could largely be explained by spatial factors. We demonstrated that historical sites can provide potential habitats for the conservation of the plant species diversity, which is based on the effectiveness of the management of their vegetation. 相似文献
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舟山本岛岩质边坡植被恢复植物多样性及群落演替 总被引：1，自引：0，他引：1
通过植被演替的时空互代,在舟山本岛选取具有代表性的不同恢复时期的矿山边坡作为研究样地,考察样地内植物物种构成特征,并根据样地内植物群落特征及不同恢复年限将其划分为4个不同的植物群落(草丛、草灌丛、灌丛、乔灌丛)演替阶段,对比分析不同演替阶段的植物多样性变化及植物群落的演替规律。结果表明:随着恢复年限的增加,多样性指数呈上升趋势,但均匀度指数则略呈下降趋势,植物群落演替总体呈良性发展趋势。岩质边坡植被恢复的研究为舟山地区或其它海岛生境条件下的岩质边坡植被重建过程中的植物配置及养护管理提供理论指导。 相似文献
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Wetland vegetation distribution modelling for the identification of constraining environmental variables 总被引：1，自引：0，他引：1
Wetland ecosystems are of primary concern for nature conservation and restoration. Adequate conservation and restoration strategies
emerge from a scientific comprehension of wetland properties and processes. Hereby, the understanding of plant species and
vegetation patterns in relation to environmental gradients is an important issue. The modelling approaches in this study statistically
relate vegetation patterns to measured environmental gradients in a lowland wetland ecosystem. Measured environmental gradients
included groundwater quantity and quality aspects, soil properties and vegetation management. Among this variety, the objective
was to identify the key environmental gradients constraining the vegetation, using recently developed methodologies within
the modelling approaches. Comparison of results indicated that different environmental gradients were considered to be important
by different methodologies. 相似文献