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丽格海棠叶片的组织培养研究 总被引:1,自引:0,他引:1
取丽格海棠叶片作外植体,通过试验,分析不同激素配比对芽的诱导、增殖及生根的影响,筛选出丽格海棠叶片组织培养的适宜培养基及试管苗最佳移栽基质。结果表明:其最佳诱导培养基为MS+6-BA1.5mg/L+NAA0.8mg/L,不定芽诱导率达100%;最佳继代培养基是MS+6-BA1.0mg/L+NAA0.5mg/L,增殖倍数达6.5倍;最佳生根培养基是1/2MS+NAA0.4mg/L,生根率达100%;试管苗最佳移栽基质为用1/2MS大量元素浇透的草炭,移栽成活率为85%。 相似文献
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颐和园古西府海棠的组织培养与快速繁殖 总被引:1,自引:0,他引:1
以北京颐和园古西府海棠嫩枝为外植体进行组织培养研究,结果表明:取西府海棠当年新生嫩茎务切段为外植体进行组织培养,其中最适不定芽启动培养基为1/2MS+IBA0.3mg/L+NAA0.05mg/L;将无菌苗接种到分化培养基MS+6-BA1.5mg/L+NAA0.4mg/L上,组培苗生长健壮且分化率高,增殖系数可达4.16;不定根最适诱导培养基为1/2MS+IBA0.6mg/L和1/2MS+NAA1.0mg/L,生根率分别达100%、98%。炼苗基质为蛭石:珍珠岩体积比:1:1。试管苗移栽成活率达92%。 相似文献
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以印楝茎尖为外植体进行离体快速繁殖,外植体采用次氯酸钠预处理,用0.1%的HgCl2浸泡消毒,共设66个处理,3次重复,分3次试验,结果表明,芽增殖培养基最佳组合为MS+6-BA 7.0g/L IAA 0.01mg/L ZT 0.1mg/L,生根培养基为1/2MS+NAA0.5mg/L,能有效促进芽生根,且移栽成活率可达80%以上。 相似文献
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红叶石楠组织培养快速繁殖技术研究 总被引:3,自引:0,他引:3
红叶石楠是一种优良的彩色观叶园林植物,采用植物组织培养方法对其快速繁殖技术进行研究。结果显示:外植体诱导培养基采用MS+6-BA0.5mg/L+IBA0.1mg/L,不定芽的诱导率达76%;增殖培养基采用MS+6-BA2.0mg/L+KT0.5mg/L+IBA0.2mg/L,丛生芽增殖率达6.32倍;生根培养基为1/2MS+NAA0.5mg/L+CCO0.5mg/L,生根率达100%。 相似文献
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文章对华灰莉木组织培养快繁育苗技术进行研究。结果表明,以华灰莉木嫩茎为外植体,不定芽增殖系数高达2.095倍,持续增殖培养时,每个继代周期约有35%~40%的不定芽可分切转生根培养。适宜华灰莉木不定芽快速增殖的培养基为MS+6-BA3.0mg/L+NAA0.1mg/L,或MS+6-BA3.5mg/L+NAA0.2mg/L,蔗糖30g/L;而诱导生根的适宜培养基为1/2MS+IBA1.0~2.0mg/L,蔗糖15g/L,生根率达94%。生根试管植株炼苗后移植存活率达90%。 相似文献
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以大扁杏龙王帽为材料,研究了外植体的消毒、基本培养基、取材部位和培养条件等因素对组培苗生长的影响。结果表明,采用1 g/L HgCl2与20 g/L NaHCO3按体积比1∶1混合的消毒合剂处理外植体8 min,灭菌成功率可达98%;以改良的MS(1/2NH4NO3)培养基为基本培养基,适合大扁杏的分化培养;最适外植体为茎尖,最佳培养条件为温度25~27℃,光照强度2 000~3 000 lx,光照时间16 h/d,pH 5.6~5.8。 相似文献
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柳桉组培快繁技术 总被引:1,自引:0,他引:1
以3年生柳桉优树环割促萌枝条为外植体,以MS为基本培养基,通过激素种类与浓度、大量元素配比等试验,探讨了柳桉腋芽诱导、继代增殖、生根培养基配方。结果表明:外植体宜采用中等幼嫩的茎段,用75%酒精消毒30 s、0.1%升汞溶液消毒4 min效果最佳;在3/4 MS+0.3 mg.L-16-BA+0.2 mg.L-1NAA培养基上进行侧芽诱导,柳桉外植体出芽率最高;在改良MS+0.2 mg.L-16-BA+0.2 mg.L-1NAA继代培养基上,柳桉增殖效果最好,其芽体健壮,生长正常,增殖率3倍以上;应用1/2MS培养基附加ABT 1#0.5 mg.L-1和IBA 0.1 mg.L-1,其生根率可达95%以上,生根3~4条,苗木生长健壮,移栽成活率可达92%以上。这些配方已成功地应用于柳桉优良无性系苗木的工厂化生产,单个超净台月苗木生产能力达7万株以上。 相似文献
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对广宁红花油茶(Camellia semiserrata)组织培养快繁育苗方法进行试验,结果表明,采用两种浓度升汞液(0.025%和0.1%)进行二次消毒的处理方法可将外植体污染率控制在50%以下。在快繁增殖阶段,培养基组分为WPM+TDZ 4.0 mg/L+6-BA 1.0 mg/L+NAA 0.3 mg/L+蔗糖30 g/L可有效诱导外植体分化丛生芽;WPM+TDZ 1.0 mg/L +6-BA1.0 mg/L +NAA 0.3 mg/L+蔗糖30 g/L可维持芽丛持续快速增殖;而WPM+6-BA 1.5 mg/L +NAA 0.5 mg/L+蔗糖30 g/L有利于促进不定芽个体生长。通常的培养方法无法诱导植株生根,但经过针对调节极性表现的程序系列培养可显著提高植株极性反应水平,调整生理反应的同步性,促使不定根分化,生根率最高达90%。再生植株移栽的平均存活率为85.4%。 相似文献
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以成熟种子为外植体建立了荻植株再生体系。荻成熟种子经20%“84”消毒液处理20min,接种于MS+4%蔗糖(pH5.5)培养基中培养5d,萌发率为92%,褐化率为8%,污染率为0。萌发种子继代转入愈伤诱导培养基MS+lmg/16-BA+1mg/12,4-D+0.5mg/lNAA+4%蔗糖(pH5.5),光照条件下愈伤组织诱导率为86%,黑暗条件下愈伤组织诱导率64%。荻愈伤组织在Ms+1IYlg,L6-BA+0.1mg/L2,4-D+0.5mg/LNAA+4%蔗糖(pH5.5)进行不定芽诱导,不定芽发生率为91%。无根丛生芽继代转入不定根诱导培养1/2MS+0.25mg/LIBA+0.25mg/LNAA+4%蔗糖(pH5.5),5天可见不定根形成,15天后不定根发生率为92%。研究还发现,荻种子萌发后可直接继代转入不定芽分化培养基形成健壮不定芽,不定芽诱导率为95%。 相似文献
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为了建立无籽刺梨组织培养技术体系,从而有效解决生产用苗紧缺问题,以无籽刺梨带叶腋的嫩茎为外植体进行了组织培养试验,研究了不同消毒时长、培养基种类及激素浓度对无籽刺梨外植体消毒效果、分化培养、增殖培养及生根培养的影响情况。结果表明:外植体经清水冲洗后用75%的酒精消毒20 s,以0.1%的升汞消毒9 min,污染率低至0;培养基1/2MS+0.50 mg/L TDZ+0.02 mg/L 2,4-D+5.0 mg/L Ag NO_3适用于无籽刺梨叶柄的分化培养,分化率为50.0%;适用于无籽刺梨增殖的培养基为MS+0.50 mg/L 6-BA+0.10 mg/L NAA,增殖倍数达5.56;在1/2MS+0.10 mg/L IBA+0.20 mg/L NAA+0.30 g/L活性炭的培养基上,无籽刺梨的生根率为92.5%;在腐殖土︰红土︰珍珠岩=1︰1︰1的基质中炼苗,无籽刺梨组培苗的成活率可达97.2%。 相似文献
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无刺枸骨具有较高的生态和观赏价值.笔者以带芽新梢茎段为外植体,研究了培养基配方、培养方式以及移栽和扦插基质等对其组培快繁的影响.结果表明:无刺枸骨芽诱导和增殖的最佳培养基为MS(或B5)+BA3.0 mg/L+IBA0.1 mg/L(或NAA0.1 mg/L)+3.0%蔗糖+0.7%琼脂;生根培养则以试管外扦插生根为好... 相似文献
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Vegetative propagation techniques are recognized as indispensable tools for mass multiplication of important multipurpose
trees adopted in different agroforestry systems. Albizia procera, one among important species, is difficult to propagate commercially either by stem / root cuttings or layering. A study
was undertaken to develop procedure for its in vitro regeneration through organogenesis. Explants collected from 15±2 yr-old mature plus trees and from 15 days old juvenile seedlings
were regenerated with exogenous application of different hormones. Epicotyl and hypocotyl explants excised from juvenile seedlings
showed higher callusing than axillary bud and shoot tip explants derived from mature trees. Benzylaminopurine (BA) at 3 μg/l
was most effective, which induced hundred percent callusing in epicotyl and hypocotyl explants in 1/2 Murashige and Skoog
(MS) medium. Callus originated from axillary buds and apical shoot tips of mature trees failed to form organs, however callus
derived from epicotyl and hypocotyl explants proliferated and formed de novo shoots and leaflets. A concentration of 3 μg/l of BA was found effective for shoot proliferation. Shoots grew vigorously
in 2 μg/l gibberellic acid (GA3) treatment and rooted in 1/2 MS medium supplemented with indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA). Rooting
was most successful on medium supplemented with 6 μg/l IBA alone on which 93.3% of the shoots formed roots. Sand or vermiculite
supplemented with 4 ml of yoshida solution proved as best hardening media, which recorded 70-80% survival of plantlets. One
year old tissue culture raised plants had comparatively more height, collar diameter, biomass, and root shoot ratio than plants
raised from cuttings and seeds of the same age. The procedures enumerated provide a basis for the development of in vitro techniques for rapid multiplication of A. procera.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献