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1.
The effects of sufficient milk intake as well as of 32 hours of fasting after birth, administration of actinomycin D (intraperitoneal application of 1 mg/kg five weight), and fasting in combination with actinomycin D on the development of body and liver weights, crude protein levels in homogenate and supernatant of liver, kidneys, and M. semitendinosus as well as on the activities of certain tissue enzymes were analysed with four groups of piglets (n = 4). Fasting, administration of actinomycin, and fasting in combination with actinomycin D resulted in rapid reduction in body and liver weights, while the crude protein levels in those tissues were not affected with significance. GOT and fructose-1,6-diphosphatase activities in supernatant from liver tended to decline under fasting conditions. The ATPase activity in the homogenate of the above tissues did not change in response to differentiated treatment. 相似文献
2.
E Kolb B Beyer K V Brenner E Grün G Gründel C Schineff U Schmidt M Stirn 《Archiv fuer experimentelle veterinaermedizin》1979,33(2):207-224
The crude protein levels as well as the activities of various enzymes were studied in certain tissues of fetuses (80th through 114th days of development), piglets of different age groups, and pigs for slaughter. In most of the tissues tested the postnatal activities of Na-K-ATPase were beyond those recorded from fetuses. The highest GOT activities were recorded from the liver, myocardium, and kidneys. Activities were found to rise sizeably in some tissues after birth. The activity of GPT, too, exhibited age-dependent variations. The activity of leucine-aminopeptidase increased strongly after birth in liver and kidneys. Acid phosphatase activity was less markedly influenced by development phases. Those enzymes which are involved in the formation of fructose and glucose (aldolreductase, glucuronate-reductase, and sorbite-dehydrogenase) had their highest activities, all age-dependent, in liver and kidneys. 相似文献
3.
选择新生仔猪15头，分别于出生当日(0 d)、出生后3d及7d屠宰取样，制作肝脏电镜切片进行组织学分析，并测定肝脏中DNA、RNA含量及常规生化指标。结果显示：新生仔猪肝细胞中内质网、线粒体等细胞器都很丰富，而且各细胞器的结构已经发育成熟。肝脏质量在仔猪出生后1周尤其是3d内迅速增加(P＜O．01)，远远快于体重增加的速度。仔猪肝脏的蛋白质含量在生后1周也迅速增加(P＜O．01)；而肝糖原贮备在刚出生时较高，出生后迅速下降(P＜O．01)。仔猪出生后肝脏中DNA和RNA浓度的变化趋势正好相反，前者在出生后3d内明显下降(P＜O．05)，4～7d又有增加趋势，而RNA浓度以3d为最高。试验结果表明，仔猪到出生时肝细胞的结构和功能基本发育成熟。仔猪出生以后，肝脏的生长发育十分迅速，不仅肝细胞的数量快速增加，肝细胞的体积也不断增大。 相似文献
4.
Modification of some hematologic and biochemical indices in mice consecutive mecadox administration.
Mecadox addition to the diet of laboratory mice in doses of 25 mg/kg/day for 30 days resulted in some hematologic and biochemical modifications: 1. Red cell count declined moderately, leucocyte and neutrophile count was higher and reached their normal values 15 days following treatment. 2. Modifications of liver transaminases expressed by a diminution of GOT and GPT activities were observed together with a reduction of free nitrogen. Values returned to normal within 15 days after treatment. 3. Mecadox stimulated weight gain in treated animals during the first 15 days of treatment. No significant differences were observed in weight gain between the two groups during the period which followed treatment. 相似文献
5.
The following parameters are recommended for activity determination of FDPase by release of inorganic phosphate at 37 degrees C of enzyme from liver and kidneys of swine: pH 7.5, 2 mMol concentration of fructose-1,6-diphosphate, 5 mMol concentration of Mg ions, and ten minutes of incubation. When the activity of FDPase was analysed with temperatures of 22 degrees C, and 45 degrees C, rises were recorded up to the highest temperature. ADTA was strongly activating along with 2 mMol of Mg ions in a test arrangement of 5 mMol concentration. Manganese, zinc, and cobalt ions are strong activators even in low concentrations (0.2 or 1.0 mMol), whereas copper, cadmium, and mercury ions are strong inhibitors of the enzyme. Average activities of FDPase were analysed by means of the optical test in the liver and kidneys of fetuses aged 98 days. They were 1.79 or 0.38 units in one gram of tissue. In pigs for slaughter they had been 2.06 or 3.58 units in one gram of tissue. 相似文献
6.
The properties of fructose-1.6-disphosphatase in supernatant of homogenate of liver, kidneys, and adductor muscles of cattle were tested. EDTA was found to activate the enzyme up to concentrations of 10 mMol. The pH optimum was 7.5 in the presence of EDTA. Even lower concentrations of magnesium ions caused activation of the enzyme, but an activating effect was obtained as well from relatively high Mg concentrations. Fructose-1.6-diphosphatase could by activated also by 0.2 mMol Mn or Co ions or 1 mMol Zn ions. Inhibitive action was obtained from Cu, Cd, Pb, and Hg ions. Microsomal fractions of cattle liver and kidney caused high activity of glucose-6-phosphatase, but only low action was obtained be using microsomal fractions of mesenteric mucous membrane or brain. Mg ions, basically, failed to trigger activation, and higher concentrations even caused inhibition. The relatively high activity of both fructose-1.6-diphosphatase and glucose-6-phosphatase in kidney of cattle appeared to suggest an involvement of those enzymes in gluconeogenesis. 相似文献
7.
家蚕丝素蛋白对大鼠胆固醇代射的影响 总被引：1，自引：0，他引：1
研究了经酸解后制得的家蚕丝素蛋白氨基酸组成以及对高血脂实验鼠血清和肝脏内胆固醇及转氨酶的活性变化的影响。结果表明：所得的家蚕丝素蛋白粉氨基酸组成丰富，其中甘氨酸、丙氨酸、丝氨酸和酪氨酸含量占全部氨基酸总量的95%；家蚕丝素蛋白能显著地降低血清胆固醇含量和谷草转氨酶及谷丙转氨酶活性，提高动物肝组织中的胆固醇浓度和谷丙转氨酶的活性。 相似文献
8.
研究了经酸解后制得的家蚕丝素蛋白氨基酸组成以及对高血脂实验鼠血清和肝脏内胆固醇及转氨酶的活性变化的影响。结果表明 :所得的家蚕丝素蛋白粉氨基酸组成丰富 ,其中甘氨酸、丙氨酸、丝氨酸和酪氨酸含量占全部氨基酸总量的 95 %;家蚕丝素蛋白能显著地降低血清胆固醇含量和谷草转氨酶及谷丙转氨酶活性 ,提高动物肝组织中的胆固醇浓度和谷丙转氨酶的活性。 相似文献
9.
乳源性生物活性肽对新生IUGR仔猪肝脏抗氧化功能的影响 总被引：2，自引：0，他引：2
本试验选择苏太初生IUGR仔猪10窝共20头,配对分为4组:胰岛素组(I组)、牛乳组(M组)、酪蛋白酶解产物组(CH组)和酪蛋白组(C组),饲喂3d后宰杀。另选5只初生IUGR仔猪立即宰杀,作为0d对照组(A组)。M组喂牛乳,I组在牛乳中添加2.5mg/L胰岛素;C组用1.5%酪蛋白水溶液取代10%(V/V)的牛乳,CH组用1.5%酪蛋白酶解液取代10%(V/V)的牛乳。宰杀后分析肝脏和血浆中SOD活性和MDA含量以及血浆中GPT和GOT水平。试验结果显示:酪蛋白酶解物可显著降低肝脏和血浆MDA含量,提高SOD活性(P<0.05),胰岛素可显著降低血浆中MDA含量(P<0.05);3日龄仔猪GPT和GOT均高于初生仔猪,其中I组、C组和CH组GPT均显著高于M组(P<0.05)。结果提示:胰岛素和酪蛋白酶解物可提高IUGR仔猪肝脏的抗氧化功能,促进肝细胞的生长发育。 相似文献
10.
家蚕后部丝腺转氨酶活性及其与茧质性状关系 总被引：1，自引：0，他引：1
采用酶学速率法分析了８个家蚕品种末龄第３、５、７日后部丝腺谷丙转氨酶（ＧＰＴ）、谷草转氨酶（ＧＯＴ）活力和比活力。少丝量品种末龄ＧＰＴ、ＧＯＴ比活力随末龄发育逐渐升高，多丝量品种ＧＰＴ比活力随末龄发育而不断下降，而ＧＯＴ比活力一直较高或略有升高。ＧＰＴ、ＧＯＴ比活力与茧层率、茧层量、全茧量的遗传相关，末龄第３天为显著正相关，第５天相关不显著，第７天为极显著负相关。ＧＯＴ、ＧＰＴ比活力遗传力分别达８７．４４％、８０．４％，可作为产丝量选择的重要生化指标。 相似文献
11.
Purified brush border membrane of Cotugnia digonopora showed the presence of a number of phosphohydrolases. Among these, alkaline phosphatase was extremely active. Other enzymes such as glucose-6-phosphatase, fructose-1,6-diphosphatase, cAMP-phosphodiesterase, 5'-nucleotidase and adenosine-triphosphatase were also active. Observations were made on the activities of various ATPases; whereas the enzyme was activated by Ca++ and Mg++ in an additive manner, its sensitivity to ouabain was negligible. Furthermore, in the presence of EDTA the enzyme activity was quite significant. The treatment of isolated brush border membrane with mebendazole, niclosamide and praziquantel in vitro did not alter the activity of these enzymes. However, treatment of intact worms drastically affected the integrity of the membrane. 相似文献
12.
通过测定小鼠血清谷草转氨酶(GOT)、谷丙转氨酶(GPT)的活性,探讨柠檬酸、Ca2+及二者联合对小鼠肝脏潜在毒性。将体重(20±2)g的昆明系小鼠100只,随机分为对照组,柠檬酸低、中、高剂量组,Ca2+低、中、高剂量组,柠檬酸联合Ca2+低、中、高剂量组,每组10只。分别经腹腔注射途径作相应处理,0.2 mL/只,对照组注射等量的生理盐水,注射2次,第2次注射7 d后,眼球取血,分离血清,分别测定血清中GOT和GPT的活性。结果显示,各组GOT活性均无显著差异(P>0.05)。与对照组GPT活性相比,钙高剂量组GPT活性升高51.66%,差异显著(P<0.05);钙中剂量组GPT活性升高42.63%,差异显著(P<0.05);联合高剂量组GPT活性升高35.32%,差异显著(P<0.05)。本研究结果表明,一定剂量的Ca2+能引起肝脏细胞损伤,与柠檬酸联合后,降低了其对肝脏的毒性。 相似文献
13.
为研究酪蛋白酶解物对胎儿宫内发育迟缓(IUGR)新生仔猪胰腺生长发育的影响,试验选择初生IUGR仔猪15头,分为新生组(N)、酪蛋白组(C)和酪蛋白酶解物组(CH),人工饲喂3d后宰杀取样。结果显示,人工饲喂3d后,IUGR仔猪胰腺重量、胰腺重/体重和胰腺蛋白质、DNA、RNA、胰高血糖素的含量以及胰淀粉酶、胰脂肪酶总活性均较新生组极显著提高(P<0.01),而胰总蛋白酶和胰蛋白酶总活性均显著下降(P<0.05),胰糜蛋白酶总活性也呈下降趋势;CH组胰糜蛋白酶总活性比C组高50.00%(P<0.01),CH组胰腺组织蛋白质、DNA含量及胰总蛋白酶、胰淀粉酶总活性也较C组不同程度提高(P>0.05)。表明IUGR仔猪生后胰腺生长发育十分迅速,酪蛋白酶解物有刺激新生IUGR仔猪胰腺胰糜蛋白酶活性提高的趋势。 相似文献
14.
选取胎次相近、体况相似、健康状况良好的长×大二元杂交空怀母猪100头,将其均分成5组,每组20头。第1、2、3、4、5组母猪被分别喂以D1、D2、D3、D4、D5试验饲粮。在饲养试验期间,测定母猪窝产仔数,仔猪初生重、成活率、断奶重,母猪产后发情间隔天数（空怀天数）、返情率等繁殖指标;并分别于母猪采食试验饲粮后5d、孕后30d、产仔后7d,每组取8头母猪前腔静脉采血,测定血清中Cl-、Na＋、HCO3-、FSH、LH、Pg含量,GPT、GOT活性等生化参数。根据各项指标测定的结果可推断：第3组母猪饲粮中阴离子的配比（即添加：NaCl 0．40％、NaHC3。0．40％）最宜;其次是第4组母猪饲粮中阴离子的配比（即添加：NaCl0．40％、NaHCO。0．80％）较宜;再次是第2组母猪饲粮中阴离子的配比（即添加：NaCl0．40％、NaHCO30．80％）。第1、5组母猪饲粮中阴离子的配比似乎不够理想,即母猪饲粮中添加的NaCl过少（0．20％）或过多（0．80％）。 相似文献
15.
16.
QFM合剂麻醉剂量对犬肝、肾功能的影响 总被引：2，自引：0，他引：2
QFM合剂是一种新型的犬用复合麻醉制剂。本试验旨在研究其对犬肝、肾功能的影响。试验用7条犬肌肉注射QFM合剂0．15～0．2mL(每千克体质量)，观察比较了注药前、后犬血清GOT、GPT、AKP活性和尿素氮、肌酐含量的变化。结果证明：注药前、后犬血清GOT、GPT、AKP活性和尿素氮、肌酐含量的变化差异不显著，在正常生理范围内。说明QFM合剂应用于犬的麻醉，时犬的肝、肾功能无明显不良影响，进一步证明了QFM合剂应用于犬的临床安全性。 相似文献
17.
It has since long been demonstrated that perinatal asphyxia in pigs can result in perinatal mortality, decreased viability/vitality at birth and reduced survival rates until 10 days of age. In human perinatology much interest is presently focussed on strategies to prevent adverse outcome arising from birth asphyxia. Given the rather high perinatal and postnatal losses in the pig industry, it is very interesting to explore rescue strategies to reduce adverse effects of postischemic organ damage caused by perinatal asphyxia also in newborn piglets.
The aim of this study was to determine the effects of postnatal treatment of piglets with 2-IminoBiotin (2-IB), a selective inhibitor of neuronal and inducible NitricOxideSynthase (nNOS and iNOS), under farm conditions on postnatal growth rates, morbidity and mortality.
In total 81 piglets from 15 litters were used. Immediately after birth, blood was collected from the umbilical artery and piglets were alternately assigned to either a control (saline) or drug (2-IB; 0.18 mg/kg bodyweight every 4 h during 24 h) group. Administration of control or drug started with an intravenous injection in the umbilical vein immediately after birth, followed by 6 intraperitoneal injections at 4 h intervals, starting 4 h after the intravenous injection. Piglets were checked for growth, morbidity and mortality until the experiment was finished (average age: 44 days).
Postnatal treatment with 2-IB resulted in significantly increased growth rates at 10 days of age (P = 0.02) (165 g/day compared to 140 g/day in controls), independently of health status and birth weight. At weaning 2-IB treated piglets tended to show higher growth rates (P = 0.06). Growth rates at the end of the experiment, morbidity and mortality were not affected by treatment.
It is hypothesized that 2-IB reduces the rather limited, nearly ‘physiological’ harmful effects occurring in the gastrointestinal tract, resulting from a short period of hypoxia-ischemia experienced during birth. This might explain the significantly increased growth rates at 10 days of life in 2-IB treated piglets. Selective inhibition of nNOS and iNOS might also result in increased availability of l-arginine for protein synthesis in newborns resulting in higher postnatal growth rates. However these issues need further investigations.
In conclusion, this study showed a positive effect of immediate postpartum administration of 2-IB during the first day after birth on growth rates up to 10 days of age. Furthermore, no negative effects of 2-IB treatment on piglet health and survival were found. 相似文献
18.
胎儿宫内发育迟缓对新生仔猪小肠组织发育的影响 总被引：3，自引：0，他引：3
为研究胎儿宫内发育迟缓(IUGR)对新生仔猪肠道生长发育的影响,比较了初生组(D0)、母乳饲喂3 d组(D3)和母乳饲喂7 d组(D7)3组中正常仔猪和宫内发育迟缓仔猪的小肠长度和重量、组织形态学结构及黏膜DNA、RNA和蛋白质含量。结果显示,初生时,新生正常仔猪小肠总重和黏膜重、RNA和蛋白质含量显著高于IUGR仔猪(P<0.05)。新生IUGR仔猪小肠的肠壁厚度、绒毛高度显著低于正常仔猪(P<0.05);而IUGR仔猪相对于体重的小肠长度极显著高于正常仔猪(P<0.01)。生长至3日龄时,正常猪小肠长度、小肠黏膜重显著高于IUGR猪(P<0.05),小肠总重、肌肉重极显著高于IUGR猪(P<0.01);IUGR仔猪小肠的肠壁厚度、绒毛高度显著低于正常仔猪(P<0.05),而IUGR仔猪相对于体重的小肠长度显著高于正常仔猪(P<0.05)。7日龄时,正常猪的小肠黏膜RNA含量显著高于IUGR猪(P<0.05),其余指标没有显著差别。整个试验结果表明宫内发育迟缓对仔猪小肠组织发育造成不利影响,导致新生仔猪小肠黏膜重量、蛋白质含量、肠壁厚度和绒毛高度的降低;IUGR仔猪的小肠组织生长在出生后1周内能部分实现补偿,而且补偿生长的实现是随着日龄增加逐步完全的。 相似文献
19.
Clinical study on experimental Theileria annulata infections of cattle. 1. Clinical-chemical studies
The extent of liver and kidney damage in 10 young steers, infected with a stabilate of Theileria annulata, was estimated by the determination of enzymes (GOT, GPT, SDH, ALD), serum levels of bilirubin and urea. At the same time the effectiveness of some liver protecting medical agents was tested. The following results were obtained: Change in the activity of the enzymes GOT, SDH and ALD and the increase of bilirubin during the advanced course of the disease are indicative of severe tissue damage in the liver. The levels of urea were found to be in the normal range with only a few exceptions. This seems to exclude the involvement of the kidney in the disease. The group of animals receiving liver protecting medication did not show any difference compared with those animals without medication. 相似文献
20.
本试验旨在研究复方中草药制剂对早期断奶仔猪蛋白质合成能力及抗应激能力的影响。选用平均体重为（7.27±0.18）kg、断奶日龄为（28±2）d的长白猪×约克夏猪仔猪126头,采用单因素完全随机区组试验设计,分为7组,每组3个重复,每个重复6头猪。日粮中中草药复方制剂剂型为水煎剂和散剂2种。试验1组为空白对照组;试验2、3、4组为水煎剂添加组,添加量分别为0.05%、0.10%、0.15%;试验5、6、7组为散剂添加组,添加量分别为0.05%、0.10%、0.15%。试验期21 d。结果表明,与对照组相比,复方中草药制剂可显著提高早期断奶仔猪血清中碱性磷酸酶（AKP）、谷草转氨酶（GOT）、谷丙转氨酶（GPT）的活力及总蛋白（TP）、白蛋白（ALB）的含量（P<0.05）,显著降低血清中尿素氮（BUN）含量（P<0.05）;显著降低早期断奶仔猪血清中肌酸激酶（CK）活力及葡萄糖（GLU）含量（P<0.05）。综上所述,在本试验条件下,复方中草药制剂能显著促进早期断奶仔猪机体蛋白质的合成,降低断奶仔猪的应激,且中草药剂型散剂优于水煎剂,最适添加比例为0.15%。 相似文献