共查询到16条相似文献，搜索用时 250 毫秒
1.
采用沙门氏菌多价血清致敏乳胶并制成乳胶抗体,建立沙门氏菌乳胶凝集试验检测方法(LAT),分别用LAT法和常规分离培养检测法对38份牛淋巴结、101份羊淋巴结进行检测.结果:乳胶凝集试验牛淋巴结阳性21份,羊淋巴结阳性44份;常规分离培养检测法牛淋巴结阳性22份,羊淋巴结阳性48份,两种方法的阳性符合率均超过80%.试验表明乳胶凝集试验操作简便、快速、敏感性高、特异性强且可用于现场检测,是一种适合基层单位检测沙门氏菌的可靠方法. 相似文献
2.
乳胶凝集试验检测成品饲料中的沙门氏菌 总被引：5，自引：0，他引：5
用沙氏菌多价血清致敏乳胶并制成乳胶抗体,建立沙氏菌乳胶凝集试验检测方法。用所建立的乳胶凝集试验方法和常规分离培养检测法检测75份成品饲料,结果乳胶凝集试验成品饲料阳性16份,常规分离培养检测法成品饲料阳性18份,两种方法的阳性符合率成品饲料为83.3%,结果表明乳胶凝集试验具有操作简便、省时、特异性强且可用于现场检测等优点,是一种适合基层单位用来检测沙门氏菌的可靠方法。 相似文献
3.
应用Dot-ELISA检测羊胴体中沙门氏菌的研究 总被引：2，自引：0，他引：2
应用Dot ELISA法对西宁某屠宰点 1 0 1份羊胴体淋巴结进行了沙门氏菌的检测 ,同时采用常规分离培养鉴定技术作为对照。结果显示在1 0 1份羊胴体中 ,Dot ELISA检出沙门氏菌阳性 56份 ,阳性率为 55 44% (56/ 1 0 1 ) ;而常规分离培养鉴定技术检出沙门氏菌阳性 48份 ,阳性率为 47 52 % (48/ 1 0 1 )。 2种方法的阳性符合率为 85 71 % ,差异不显著 (P >0 0 5)。 相似文献
4.
以硝酸纤维膜为固相载体 ,利用沙门氏菌多价血清和辣根过氧化物酶 (HRP)标记制备的羊抗兔IgG ,成功地建立对火腿肠中沙门氏菌的Dot ELISA检测法 ,同时 ,采用常规分离培养鉴定技术作为对照试验 ,对 80份火腿肠进行了沙门氏菌的检测。结果显示 ,在 80份火腿肠中 ,用Dot ELISA检出沙门氏菌阳性为 2 2份 ,阳性率为 2 7 3 % (2 2 80 ) ;而常规分离培养鉴定技术检出沙门氏菌阳性为 2 0份 ,阳性率 2 5 0 0 % (2 0 80 ) ,两种方法的阳性符合率为 86 3 6% (P >0 0 5 )。 相似文献
5.
为了解甘肃省兰州市城关区犬布鲁氏菌病的流行情况，从兰州市城关区动物医疗机构和兰州市流浪动物救助站共采集犬血清343份，采用光滑型虎红平板凝集试验、粗糙型虎红平板凝集试验、通用型胶体金检测法和光滑型胶体金检测法进行检测，对4种方法检测出的阳性血清进行通用型快速PCR复检；对光滑型虎红平板和粗糙型虎红平板凝集试验检测出的阳性血清再分别进行光滑型试管凝集试验和粗糙型试管凝集试验复检。结果表明，4种方法共检出34份阳性血清，其中抗体检测法虎红平板凝集试验、试管凝集试验及布鲁氏菌通用型抗体检测试纸条分别检测出阳性血清22份、18份和12份，抗体阳性率分别为6.4%、5.2%和3.5%;抗原检测法通用型快速PCR检出阳性血清25份，抗原阳性率为7.2%。22份虎红平板凝集阳性样本经光滑型试管凝集试验和粗糙型试管凝集试验复检，共检出阳性血清18份，且均为粗糙型；布鲁氏菌光滑性抗体金标快速检测卡检测均为阴性。说明兰州市城关区犬感染的主要是粗糙型布鲁氏菌，快速通用型荧光PCR的阳性检出率最高。 相似文献
6.
Dot-ELISA检测猪胴体中沙门氏菌的研究 总被引：10，自引：0，他引：10
应用Dot-ELISA法对西宁某猪屠宰点 85份猪胴体进行了沙门氏菌的检测 ,同时采用常规分离培养鉴定技术作对照实验。结果在 85份肉样中 ,Dot-ELISA检出沙门氏菌阳性 6 5份 ,阳性率为 76 .4 7% (6 5 /85 ) ;而常规分离培养鉴定技术检出沙门氏菌阳性 6 7份 ,阳性率为 78.82 % (6 7/85 ) ,此两种方法的阳性符合率为 86 .5 7%。经统计分析 ,两种方法差异不显著 (P >0 .0 5 )。 相似文献
7.
目前,调查分析了张家川县2015—2020年家畜牛、羊布鲁氏菌病的流行情况。采用虎红平板凝胶实验方法、试管凝集试验、PCR检测法对2015—2020年全县15个乡镇的牛羊进行了布鲁氏菌病血清学检测,其中牛血清9 925份,羊血清70 992份,采用虎红平板凝集试验和试管凝集试验检测牛羊血清80 917份,采用PCR检测法检测血清7份。通过对2015年至2020年牛、羊布鲁氏菌病阳性检出率的分析,说明张家川县的布鲁氏菌病在逐年的净化之中,流行处于较低水平,但是动物防疫部门的防控压力仍旧很大,需要加大实验室检测力度、落实检疫监管与移动控制、建设专业队伍及人员培训等方面着手,形成工作合力,有效避免从疫病源上传染给人。 相似文献
8.
以硝酸纤维膜为固相载体,利用沙门氏菌多价血清和辣根过氧化物酶(HRP)标记制备的羊抗兔IgG,成功地建立对香肠的Dot-ELISA检查法,同时,采用常规分离培养鉴定技术作对照试验。结果显示,在86份香肠中,用Dot-ELISA检出沙门氏菌阳性为36份,阳性率为41.86%;而常规分离培养鉴定技术检出沙门氏菌阳性为34份,阳性率为39.53%,两种方法的阳性符合率为86.17%,经统计分析,t=0.311 1,p>0.05,两种方法差异不显著。 相似文献
9.
沙门氏菌是一种常见的人畜共患性病原菌，EastAnglia大学的研究表明，沙门氏菌在适宜条件下能迅速繁殖，对人和动物的健康构成严重的威胁。人的大多数沙门氏菌感染直接或间接地与所食的动物性食品有关，且致病因素复杂多变。近年来，人和动物的沙门氏菌感染十分严重。为此，许多学者致力于沙门氏菌检验方法的研究，目前已有细菌的分离培养鉴定技术、常规ELISA法、SPA-协同凝集试验和PCR。在继承了常规ELISA方法优点的基础上，在20世纪80年代初建立和发展了一种新型的免疫酶标记技术，即酶联免疫吸附试验(Dot-ELISA检测法)。应用斑点酶联免疫吸附试验诊断方法快速检测羊胴体中的沙门氏菌与常规分离培养检测比较，不仅简便，快捷，而且安全、可靠。现将此法介绍如下。 相似文献
10.
《青海畜牧兽医杂志》2017,(2)
应用虎红平板和试管凝集试验对3607份来自格尔木地区牛、羊、鼠兔、旱獭、田鼠和小家鼠的血清样品进行布氏杆菌病特异性抗体的检测。结果用虎红平板检出阳性血清66份,血清阳性率为1.83%;用试管凝集试验检出阳性14份,阳性率为0.39%,说明在格尔木地区的野生动物群中存在布氏杆菌病的感染。 相似文献
11.
One jejunal and one caecal lymph node were sampled from each of 50 cows, 40 yearling cattle, 25 sheep, 20 lambs and 45 pigs after slaughter. Salmonella, Clostridium perfringens and Staphylococcus aureus , all organisms which cause food poisoning in man, were sought by direct plating methods. The samples were also enriched and cultured for Salmonella. Organisms were cultured from 208 (58%) of the 360 lymph nodes; aerobic plate counts yielded up to 25,000 organisms per gram of tissue, although from most infected samples less than 1000 organisms per gram were cultured. Salmonella was isolated directly from 5% of samples, with counts up to 1,500 per gram. After enrichment Salmonella was isolated from nodes taken from 15 cows, 2 yearling cattle, one sheep and 8 pigs. Cl. perfringens was isolated from the caecal nodes of 2 yearling cattle and 2 pigs; S. aureus was not isolated from any sample. It was concluded that mesenteric lymph nodes may be a significant reservoir of Salmonella for transfer to meat and meat products. 相似文献
12.
Pavlik I Matlova L Bartl J Svastova P Dvorska L Whitlock R 《Veterinary microbiology》2000,77(3-4):309-324
Faecal (at least 3 months before slaughtering) and organ examinations were carried out in 611 animals (497 dairy, 69 dual-purpose and 44 beef cattle) originating from eight paratuberculosis infected cattle herds. The diagnosis in cattle was established by routine intestinal culture (ileum and the adjacent lymph nodes) after slaughter. In selected 132 animals, post-mortem intensive culture was performed on tissue samples collected from the gastrointestinal tract (duodenum, jejunum, ileum, ileocecal valve, caecum, rectum) and the corresponding lymph nodes, submandibular, retropharyngeal, tracheobronchial, liver and supramammary lymph nodes, kidney, liver and spleen. In 251 (41.1%) of all 611 animals, Mycobacterium avium subspecies paratuberculosis could be isolated from the faeces; in 164 (65.7%) out of 251 shedding animals the infection was detected in the ileum and adjacent lymph nodes. The detection of M. paratuberculosis by routine intestinal culture of faecal culture positive animals varied from 46.0% in animals shedding 1 CFU (colony forming unit), to 94.7% in massive shedders. On the contrary, M. paratuberculosis was detected by routine intestinal culture in 92 (25.5%) of the 360 faecal culture negative animals. Shedding animals had significantly higher (P<0.01) number of organisms in their organs than non-shedding animals. During the intensive tissue cultivation from selected 132 animals, 72 (54.5%) of them were positive. For the negative animals, no significant difference was found between the detection rate in organs examined after slaughter with routine and intensive method. However, in the subgroup of tissue culture positive animals a highly significant difference (P<0.01) was found by intensive examination (83.0%) compared with the routine examination (60.4%). Out of 72 tissue culture positive animals 73.6% of them harboured M. paratuberculosis in the gastrointestinal tract, 16.7% in the gastrointestinal tract and the parenchymatous organs, tracheobronchial and mandibular lymph nodes. The rest of the 9.7% of the infection was detected in the lymph nodes of head and lungs. Our study concerning the distribution of M. paratuberculosis by intensive examinations revealed a minimum effect of breed and production type on localisation of the agent. Thus, the results suggest that in case of an active infection, M. paratuberculosis can be localised in different organs of animals irrespective of their breed or production type. 相似文献
13.
Development of latex agglutination test with recombinant NcSAG1 for the rapid detection of antibodies to Neospora caninum in cattle 总被引：1，自引：0，他引：1
Moraveji M Hosseini A Moghaddar N Namavari MM Eskandari MH 《Veterinary parasitology》2012,189(2-4):211-217
Neospora caninum, an apicomplexan protozoan parasite, is recognized as a major cause of abortion in cattle. Surface antigen 1 of N. caninum (NcSAG1) is an important immunodominant candidate for the development of a diagnostic reagent for neosporosis. The present study describes the development and evaluation of a latex agglutination test (LAT) with recombinant NcSAG1 (rNcSAG1) for the detection of antibodies to N. caninum in cattle. The rNcSAG1 gene was cloned in pET-28a and protein was expressed in Escherichia coli BL21 (DE3). Carboxylated latex particles were coated with rNcSAG1 and the degree of agreement between LAT and a commercial enzyme-linked immunosorbent assay (iscomELISA) was evaluated by using of 164 serum samples. Twenty-two (13.4%) and 23 (14.0%) of samples were positive for antibodies to N. caninum by LAT and ELISA respectively. Eighteen of 23 ELISA-positive samples were positive according to the LAT and a substantial agreement (κ=0.77) was found between the results of LAT and ELISA. The results indicated that the LAT with rNcSAG1 would be a rapid, simple, relatively inexpensive and suitable diagnostic test for detection of specific antibodies in N. caninum infection under field conditions. Improvement in purification of rNcSAG1 can reduce probable false positive reactions and so increase the degree of agreement between the LAT and ELISA. 相似文献
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15.
Detailed postmortem examinations were conducted to evaluate the efficiency of meat inspection procedures and to determine the distribution of lesions in Mycobacterium bovis-infected cattle. The study involved routine inspection at slaughter, collection of tissues for detailed examination in the laboratory, and bacteriological examination to identify M. bovis. Additionally, a 10-year (1992--2001) meat inspection record was analysed to determine tuberculosis trends in the past decade. chi2-Test and simple regression were used to analyse the data. Out of 1350 cattle examined, 1.5% were found with tuberculous lesions. Routine abattoir inspection detected only 55% of cattle with confirmed lesions. Fifty-four per cent of tuberculous lesions were found in the lungs and thoracic lymph nodes, 23% in the lymph nodes of the head, and the remaining 23% in the mesenteric and other lymph nodes of the carcase. M. bovis was additionally isolated from an animal that had no gross lesions of tuberculosis. On average, the annual rate of whole-carcase condemnation due to generalized tuberculosis was 0.024% and it has increased annually by 0.34% over the past decade. The rate of whole-carcase condemnation indicates a high degree of TB transmission and requires immediate attention from both the economic and public health points of view. The lower sensitivity of routine abattoir inspection confirms the importance of improving necropsy procedures. 相似文献
16.
Serological investigation of aborted sheep and pigs for infection by Neospora caninum 总被引：4，自引：0，他引：4
Serology for Neospora caninum was undertaken using direct ELISAs on sera from 660 aborted sheep and 454 breeding sows, which had aborted or were considered infertile. All ovine sera were further tested by indirect fluorescent antibody test (IFAT) for N. caninum, and a latex agglutination test (LAT) for Toxoplasma gondii was performed on 423 of the samples, including all those positive by ELISA. ELISA-positive porcine sera were tested by IFAT and an inhibition ELISA for antibodies to N. caninum and by LAT for T. gondii. Only 3 (0.45%) of the ovine sera were seropositive for N. caninum by both ELISA and IFAT whereas although 40 porcine sera were seropositive by ELISA all were negative by IFAT. The results suggest that environmental exposure to N. caninum occurs rarely in sheep and pigs. 相似文献